P40483 (YIK8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative zinc metalloproteinase YIL108W
EC=3.4.24.-

Gene names

Ordered Locus Names:

YIL108W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	696 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Cofactor	Binds 1 zinc ion By similarity.
Subcellular location	Cytoplasm Ref.4.
Miscellaneous	Present with 195 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the peptidase M10B family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from direct assay Ref.4PubMed 22842922. Source: SGD
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 696	696	Putative zinc metalloproteinase YIL108W		PRO_0000078181
Sites
	Active site	319	1	By similarity
	Metal binding	318	1	Zinc; catalytic By similarity
	Metal binding	322	1	Zinc; catalytic By similarity
	Metal binding	328	1	Zinc; catalytic By similarity
Amino acid modifications
	Modified residue	361	1	Phosphoserine Ref.6
Experimental info
	Sequence conflict	696	1	L → F in AAT92711. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P40483 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 89ED15503933653B	FASTA	696	77,414
	10 20 30 40 50 60 MVGSKDIDLF NLRENEQIVS PCLIVHGKCN KQNGAKTVQV QHPQLPPITY PIHNQFFKAT 70 80 90 100 110 120 VILTPGENKL TFVTDTNTAR TIVCYYTPLT QNPPVHLCLI LAKDSPLQFD SPREQKDREG 130 140 150 160 170 180 GNGLELAIKK LRLGARLMQA YTNEQMLRNS MGNRTFPFVE EFTWDTLFER PAMRNTIKIH 190 200 210 220 230 240 VVRSEKTVKE IQDPDIAQQN SKGKNTGALF GIAMDALKSY GGPFTNNEKP VQAACMFLDT 250 260 270 280 290 300 HWDGKLIRGH AALGGGDDSI KLAIFGSHGL YSWPTCLEQL VPYFTDETRS STSEVANDCN 310 320 330 340 350 360 ECGTYWECLT ITLGAFMHEI GHLLGCPHQE SGVMLRGYTT LNRSFLTKEA YSVRTNSTGA 370 380 390 400 410 420 SPPIFPKEEC TWNRLDTVRF LYHPSFTLPQ DYYDPSFMRP TKLGGYPNIK HSVYPLGNGS 430 440 450 460 470 480 CRILSPTGIY LIEIICDDLA RGHIEYLPVS LGGQGPQREV IVTLDDLRAR LPKNELAKFG 490 500 510 520 530 540 NTFKLKILSV NAPETEFDKF PSLLDVQPLD MSKYGFSKNV QGIKSPLYGR SDGGNAVGVV 550 560 570 580 590 600 AFDVRLVTAV RIYHGYALDG VRFYYKEKPT GTKDAPASKP SVPPRNYFSK ITHSIKNHAS 610 620 630 640 650 660 INEENLKSVL FGHETQNFTD ATLEPGEIII GFNLRCGAWV DAIQIITSHG RMTDMFGNKD 670 680 690 GGGFAELQPP NGQYILGVTG RVGQWVDAFG IIYGAL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-361, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z38125 Genomic DNA. Translation: CAA86272.1. AY692692 Genomic DNA. Translation: AAT92711.1. BK006942 Genomic DNA. Translation: DAA08445.1.
PIR	S48464.
RefSeq	NP_012158.1. NM_001179456.1.
3D structure databases
ProteinModelPortal	P40483.
SMR	P40483. Positions 314-342, 621-695.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5657N.
IntAct	P40483. 4 interactions.
MINT	MINT-479748.
STRING	4932.YIL108W.
Proteomic databases
PaxDb	P40483.
PeptideAtlas	P40483.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIL108W; YIL108W; YIL108W.
GeneID	854698.
KEGG	sce:YIL108W.
Organism-specific databases
CYGD	YIL108w.
SGD	S000001370. YIL108W.
Phylogenomic databases
eggNOG	NOG283285.
HOGENOM	HOG000197526.
KO	K01417.
OMA	YLWQAFT.
OrthoDB	EOG4643M7.
Gene expression databases
Genevestigator	P40483.
GermOnline	YIL108W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	2.100.10.30. 2 hits. 3.40.390.10. 1 hit.
InterPro	IPR001229. Mannose-bd_lectin. IPR024079. MetalloPept_cat_dom. IPR021917. Unchr_Zn-peptidase-like. [Graphical view]
Pfam	PF01419. Jacalin. 1 hit. PF12044. Metallopep. 1 hit. [Graphical view]
SUPFAM	SSF51101. Mannose-bd_lectin. 1 hit.
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977340.

Entry information

Entry name

YIK8_YEAST

Accession

Primary (citable) accession number: P40483
Secondary accession number(s): D6VVH9, Q6B2N8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program