P40479 (SDP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dual-specificity protein phosphatase SDP1
EC=3.1.3.48

Alternative name(s):
Stress-inducible MAPK phosphatase

Gene names

Name:	SDP1
Ordered Locus Names:	YIL113W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	209 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mediates dephosphorylation of MAPK substrates such as SLT2, acquiring enhanced catalytic activity under oxidative conditions. Ref.3 Ref.4
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

Ontologies

Keywords
Molecular function	Hydrolase Protein phosphatase
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	MAPK cascade involved in cell wall biogenesis Inferred from genetic interaction PubMed 11923319. Source: SGD adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion Inferred from Biological aspect of Ancestor. Source: RefGenome inactivation of MAPK activity involved in cell wall biogenesis Inferred from mutant phenotype Ref.3. Source: SGD regulation of pheromone-dependent signal transduction involved in conjugation with cellular fusion Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	cytosol Inferred from Biological aspect of Ancestor. Source: RefGenome nucleus Inferred from direct assay PubMed 11923319. Source: SGD
Molecular_function	MAP kinase tyrosine phosphatase activity Inferred from direct assay PubMed 11923319Ref.4. Source: SGD MAP kinase tyrosine/serine/threonine phosphatase activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 209

209

Dual-specificity protein phosphatase SDP1

PRO_0000094924

Sites

Active site

140

1

Phosphocysteine intermediate

Binding site

111

1

Phosphotyrosine

Amino acid modifications

Disulfide bond

47 ↔ 142

Ref.4

Secondary structure

1

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209

Helix Strand Turn

Details...

Beta strand

58 – 64

7

Turn

65 – 67

3

Beta strand

68 – 73

6

Turn

79 – 81

3

Beta strand

83 – 87

5

Turn

96 – 98

3

Beta strand

102 – 106

5

Helix

115 – 118

4

Helix

119 – 131

13

Beta strand

136 – 140

5

Beta strand

141 – 145

5

Helix

146 – 158

13

Helix

163 – 173

11

Helix

181 – 195

15

Sequences

	Sequence		Length	Mass (Da)
	P40479 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 678371462A890A69	FASTA	209	23,886
	10 20 30 40 50 60 MNIYTSPTRT PNIAPKSGQR PSLPMLATDE RSTDKESPNE DREFVPCSSL DVRRIYPKGP 70 80 90 100 110 120 LLVLPEKIYL YSEPTVKELL PFDVVINVAE EANDLRMQVP AVEYHHYRWE HDSQIALDLP 130 140 150 160 170 180 SLTSIIHAAT TKREKILIHC QCGLSRSATL IIAYIMKYHN LSLRHSYDLL KSRADKINPS 190 200 IGLIFQLMEW EVALNAKTNV QANSYRKVP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"YIL113w encodes a functional dual-specificity protein phosphatase which specifically interacts with and inactivates the Slt2/Mpk1p MAP kinase in S. cerevisiae." Collister M., Didmon M.P., MacIsaac F., Stark M.J., MacDonald N.Q., Keyse S.M. FEBS Lett. 527:186-192(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"Redox-mediated substrate recognition by Sdp1 defines a new group of tyrosine phosphatases." Fox G.C., Shafiq M., Briggs D.C., Knowles P.P., Collister M., Didmon M.J., Makrantoni V., Dickinson R.J., Hanrahan S., Totty N., Stark M.J., Keyse S.M., McDonald N.Q. Nature 447:487-492(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 17-198 OF WILD TYPE AND MUTANT SER-142, FUNCTION, DISULFIDE BOND.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z38125 Genomic DNA. Translation: CAA86267.1.
BK006942 Genomic DNA. Translation: DAA08440.1.

PIR

S48459.

RefSeq

NP_012153.1. NM_001179461.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2J16	X-ray	2.70	A/B	17-198	[»]
2J17	X-ray	2.84	A/B	17-198	[»]

ProteinModelPortal

P40479.

SMR

P40479. Positions 54-196.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1899N.

IntAct

P40479. 5 interactions.

MINT

MINT-395807.

STRING

4932.YIL113W.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YIL113W; YIL113W; YIL113W.

GeneID

854693.

KEGG

sce:YIL113W.

Organism-specific databases

CYGD

YIL113w.

SGD

S000001375. SDP1.

Phylogenomic databases

eggNOG

COG2453.

GeneTree

ENSGT00680000100889.

KO

K01104.

OMA

MEWEVAL.

OrthoDB

EOG45B4R5.

Gene expression databases

Genevestigator

P40479.

GermOnline

YIL113W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]

PANTHER

PTHR10159. PTHR10159. 1 hit.

Pfam

PF00782. DSPc. 1 hit.
[Graphical view]

SMART

SM00195. DSPc. 1 hit.
[Graphical view]

PROSITE

PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P40479.

NextBio

977325.

Entry information

Entry name

SDP1_YEAST

Accession

Primary (citable) accession number: P40479
Secondary accession number(s): D6VVH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program