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P40479 (SDP1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dual-specificity protein phosphatase SDP1

EC=3.1.3.48
Alternative name(s):
Stress-inducible MAPK phosphatase
Gene names
Name:SDP1
Ordered Locus Names:YIL113W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length209 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mediates dephosphorylation of MAPK substrates such as SLT2, acquiring enhanced catalytic activity under oxidative conditions. Ref.3 Ref.5

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sequence similarities

Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 209209Dual-specificity protein phosphatase SDP1
PRO_0000094924

Sites

Active site1401Phosphocysteine intermediate
Binding site1111Phosphotyrosine

Amino acid modifications

Disulfide bond47 ↔ 142 Ref.5

Secondary structure

........................ 209
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P40479 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 678371462A890A69

FASTA20923,886
        10         20         30         40         50         60 
MNIYTSPTRT PNIAPKSGQR PSLPMLATDE RSTDKESPNE DREFVPCSSL DVRRIYPKGP 

        70         80         90        100        110        120 
LLVLPEKIYL YSEPTVKELL PFDVVINVAE EANDLRMQVP AVEYHHYRWE HDSQIALDLP 

       130        140        150        160        170        180 
SLTSIIHAAT TKREKILIHC QCGLSRSATL IIAYIMKYHN LSLRHSYDLL KSRADKINPS 

       190        200 
IGLIFQLMEW EVALNAKTNV QANSYRKVP 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"YIL113w encodes a functional dual-specificity protein phosphatase which specifically interacts with and inactivates the Slt2/Mpk1p MAP kinase in S. cerevisiae."
Collister M., Didmon M.P., MacIsaac F., Stark M.J., MacDonald N.Q., Keyse S.M.
FEBS Lett. 527:186-192(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[5]"Redox-mediated substrate recognition by Sdp1 defines a new group of tyrosine phosphatases."
Fox G.C., Shafiq M., Briggs D.C., Knowles P.P., Collister M., Didmon M.J., Makrantoni V., Dickinson R.J., Hanrahan S., Totty N., Stark M.J., Keyse S.M., McDonald N.Q.
Nature 447:487-492(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 17-198 OF WILD TYPE AND MUTANT SER-142, FUNCTION, DISULFIDE BOND.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z38125 Genomic DNA. Translation: CAA86267.1.
BK006942 Genomic DNA. Translation: DAA08440.1.
PIRS48459.
RefSeqNP_012153.1. NM_001179461.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2J16X-ray2.70A/B17-198[»]
2J17X-ray2.84A/B17-198[»]
ProteinModelPortalP40479.
SMRP40479. Positions 54-196.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34878. 18 interactions.
DIPDIP-1899N.
IntActP40479. 4 interactions.
MINTMINT-395807.
STRING4932.YIL113W.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYIL113W; YIL113W; YIL113W.
GeneID854693.
KEGGsce:YIL113W.

Organism-specific databases

CYGDYIL113w.
SGDS000001375. SDP1.

Phylogenomic databases

eggNOGCOG2453.
GeneTreeENSGT00680000100889.
KOK01104.
OMANCAREIP.
OrthoDBEOG7HF1WG.

Enzyme and pathway databases

BioCycYEAST:G3O-31367-MONOMER.

Gene expression databases

GenevestigatorP40479.

Family and domain databases

Gene3D3.90.190.10. 1 hit.
InterProIPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERPTHR10159. PTHR10159. 1 hit.
PfamPF00782. DSPc. 1 hit.
[Graphical view]
SMARTSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMSSF52799. SSF52799. 1 hit.
PROSITEPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP40479.
NextBio977325.

Entry information

Entry nameSDP1_YEAST
AccessionPrimary (citable) accession number: P40479
Secondary accession number(s): D6VVH4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references