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P40479

- SDP1_YEAST

UniProt

P40479 - SDP1_YEAST

Protein

Dual-specificity protein phosphatase SDP1

Gene

SDP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Mediates dephosphorylation of MAPK substrates such as SLT2, acquiring enhanced catalytic activity under oxidative conditions.2 Publications

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei111 – 1111Phosphotyrosine
    Active sitei140 – 1401Phosphocysteine intermediate

    GO - Molecular functioni

    1. MAP kinase tyrosine phosphatase activity Source: SGD
    2. protein tyrosine/serine/threonine phosphatase activity Source: InterPro

    GO - Biological processi

    1. inactivation of MAPK activity involved in cell wall organization or biogenesis Source: SGD
    2. MAPK cascade involved in cell wall organization or biogenesis Source: SGD
    3. peptidyl-tyrosine dephosphorylation involved in inactivation of protein kinase activity Source: GOC

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31367-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dual-specificity protein phosphatase SDP1 (EC:3.1.3.48)
    Alternative name(s):
    Stress-inducible MAPK phosphatase
    Gene namesi
    Name:SDP1
    Ordered Locus Names:YIL113W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    CYGDiYIL113w.
    SGDiS000001375. SDP1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. nucleus Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 209209Dual-specificity protein phosphatase SDP1PRO_0000094924Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi47 ↔ 1421 Publication

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Gene expression databases

    GenevestigatoriP40479.

    Interactioni

    Protein-protein interaction databases

    BioGridi34878. 18 interactions.
    DIPiDIP-1899N.
    IntActiP40479. 4 interactions.
    MINTiMINT-395807.
    STRINGi4932.YIL113W.

    Structurei

    Secondary structure

    1
    209
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi58 – 647
    Turni65 – 673
    Beta strandi68 – 736
    Turni76 – 816
    Beta strandi83 – 875
    Helixi96 – 983
    Beta strandi103 – 1064
    Helixi112 – 1187
    Helixi119 – 13113
    Beta strandi136 – 1427
    Helixi146 – 15813
    Helixi163 – 17311
    Helixi181 – 19515

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2J16X-ray2.70A/B17-198[»]
    2J17X-ray2.84A/B17-198[»]
    ProteinModelPortaliP40479.
    SMRiP40479. Positions 54-196.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP40479.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG2453.
    GeneTreeiENSGT00680000100889.
    KOiK01104.
    OMAiNCAREIP.
    OrthoDBiEOG7HF1WG.

    Family and domain databases

    Gene3Di3.90.190.10. 1 hit.
    InterProiIPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view]
    PANTHERiPTHR10159. PTHR10159. 1 hit.
    PfamiPF00782. DSPc. 1 hit.
    [Graphical view]
    SMARTiSM00195. DSPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 1 hit.
    PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40479-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNIYTSPTRT PNIAPKSGQR PSLPMLATDE RSTDKESPNE DREFVPCSSL    50
    DVRRIYPKGP LLVLPEKIYL YSEPTVKELL PFDVVINVAE EANDLRMQVP 100
    AVEYHHYRWE HDSQIALDLP SLTSIIHAAT TKREKILIHC QCGLSRSATL 150
    IIAYIMKYHN LSLRHSYDLL KSRADKINPS IGLIFQLMEW EVALNAKTNV 200
    QANSYRKVP 209
    Length:209
    Mass (Da):23,886
    Last modified:February 1, 1995 - v1
    Checksum:i678371462A890A69
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z38125 Genomic DNA. Translation: CAA86267.1.
    BK006942 Genomic DNA. Translation: DAA08440.1.
    PIRiS48459.
    RefSeqiNP_012153.1. NM_001179461.1.

    Genome annotation databases

    EnsemblFungiiYIL113W; YIL113W; YIL113W.
    GeneIDi854693.
    KEGGisce:YIL113W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z38125 Genomic DNA. Translation: CAA86267.1 .
    BK006942 Genomic DNA. Translation: DAA08440.1 .
    PIRi S48459.
    RefSeqi NP_012153.1. NM_001179461.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2J16 X-ray 2.70 A/B 17-198 [» ]
    2J17 X-ray 2.84 A/B 17-198 [» ]
    ProteinModelPortali P40479.
    SMRi P40479. Positions 54-196.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34878. 18 interactions.
    DIPi DIP-1899N.
    IntActi P40479. 4 interactions.
    MINTi MINT-395807.
    STRINGi 4932.YIL113W.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIL113W ; YIL113W ; YIL113W .
    GeneIDi 854693.
    KEGGi sce:YIL113W.

    Organism-specific databases

    CYGDi YIL113w.
    SGDi S000001375. SDP1.

    Phylogenomic databases

    eggNOGi COG2453.
    GeneTreei ENSGT00680000100889.
    KOi K01104.
    OMAi NCAREIP.
    OrthoDBi EOG7HF1WG.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31367-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P40479.
    NextBioi 977325.

    Gene expression databases

    Genevestigatori P40479.

    Family and domain databases

    Gene3Di 3.90.190.10. 1 hit.
    InterProi IPR000340. Dual-sp_phosphatase_cat-dom.
    IPR020422. Dual-sp_phosphatase_subgr_cat.
    IPR024950. DUSP.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view ]
    PANTHERi PTHR10159. PTHR10159. 1 hit.
    Pfami PF00782. DSPc. 1 hit.
    [Graphical view ]
    SMARTi SM00195. DSPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 1 hit.
    PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "YIL113w encodes a functional dual-specificity protein phosphatase which specifically interacts with and inactivates the Slt2/Mpk1p MAP kinase in S. cerevisiae."
      Collister M., Didmon M.P., MacIsaac F., Stark M.J., MacDonald N.Q., Keyse S.M.
      FEBS Lett. 527:186-192(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    5. Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 17-198 OF WILD TYPE AND MUTANT SER-142, FUNCTION, DISULFIDE BOND.

    Entry informationi

    Entry nameiSDP1_YEAST
    AccessioniPrimary (citable) accession number: P40479
    Secondary accession number(s): D6VVH4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3