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Protein

Nucleoporin NUP159

Gene

NUP159

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Active directional transport is assured by both, a Phe-Gly (FG) repeat affinity gradient for these transport factors across the NPC and a transport cofactor concentration gradient across the nuclear envelope (GSP1 and GSP2 GTPases associated predominantly with GTP in the nucleus, with GDP in the cytoplasm). NUP159 plays an important role in several nuclear export pathways including poly(A)+ RNA, pre-ribosome, and protein export.13 Publications

GO - Molecular functioni

  • adenyl-nucleotide exchange factor activity Source: SGD
  • nucleocytoplasmic transporter activity Source: SGD

GO - Biological processi

  • ncRNA export from nucleus Source: SGD
  • NLS-bearing protein import into nucleus Source: SGD
  • nuclear pore distribution Source: SGD
  • poly(A)+ mRNA export from nucleus Source: SGD
  • protein export from nucleus Source: SGD
  • ribosomal large subunit export from nucleus Source: SGD
  • ribosomal small subunit export from nucleus Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31369-MONOMER.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin NUP159
Alternative name(s):
Nuclear pore protein NUP159
Gene namesi
Name:NUP159
Synonyms:NUP158, RAT7
Ordered Locus Names:YIL115C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL115C.
SGDiS000001377. NUP159.

Subcellular locationi

GO - Cellular componenti

  • nuclear membrane Source: UniProtKB-SubCell
  • nuclear pore Source: SGD
  • nuclear pore central transport channel Source: SGD
  • nuclear pore cytoplasmic filaments Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002048491 – 1460Nucleoporin NUP159Add BLAST1460

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei404PhosphoserineCombined sources1
Modified residuei657PhosphoserineCombined sources1
Modified residuei724PhosphoserineCombined sources1
Modified residuei735PhosphoserineCombined sources1
Modified residuei745PhosphoserineCombined sources1
Modified residuei803PhosphothreonineCombined sources1
Modified residuei805PhosphoserineCombined sources1
Modified residuei819PhosphoserineCombined sources1
Modified residuei889PhosphoserineCombined sources1
Modified residuei940PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40477.
PRIDEiP40477.

PTM databases

iPTMnetiP40477.

Interactioni

Subunit structurei

The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. NUP159 is part of the NUP82 subcomplex (NUP82, NSP1, NUP159) interacting with NUP82 through its C-terminal coiled coil. This subcomplex is the base for interactions with NUP116 and GLE2 and with NUP42 and GLE1. Interacts through its FG repeats with karyopherins, such as heterodimeric mRNA transport factor MEX67/MTR2, CRM1 (XPO1), and PSE1 (GSP1-GDP dependent). Interaction with CRM1 (XPO1) is GSP1-GTP dependent and stimulated by RNA1. NUP159 also interacts with GLE1 and the ATP-dependent RNA helicase DBP5.10 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
KAP95Q061423EBI-11747,EBI-9145
NUP82P403686EBI-11747,EBI-12331

Protein-protein interaction databases

BioGridi34876. 92 interactors.
DIPiDIP-2314N.
IntActiP40477. 17 interactors.
MINTiMINT-629459.

Structurei

Secondary structure

11460
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi10 – 15Combined sources6
Beta strandi17 – 25Combined sources9
Beta strandi42 – 45Combined sources4
Turni46 – 49Combined sources4
Beta strandi50 – 55Combined sources6
Beta strandi58 – 63Combined sources6
Helixi64 – 72Combined sources9
Beta strandi73 – 75Combined sources3
Beta strandi81 – 85Combined sources5
Beta strandi89 – 95Combined sources7
Beta strandi98 – 114Combined sources17
Beta strandi118 – 123Combined sources6
Beta strandi128 – 133Combined sources6
Beta strandi135 – 142Combined sources8
Beta strandi145 – 151Combined sources7
Turni152 – 154Combined sources3
Beta strandi157 – 169Combined sources13
Beta strandi171 – 178Combined sources8
Beta strandi183 – 189Combined sources7
Beta strandi192 – 199Combined sources8
Helixi203 – 206Combined sources4
Turni210 – 212Combined sources3
Beta strandi213 – 231Combined sources19
Beta strandi245 – 253Combined sources9
Beta strandi256 – 265Combined sources10
Beta strandi278 – 284Combined sources7
Beta strandi286 – 288Combined sources3
Beta strandi292 – 298Combined sources7
Beta strandi305 – 307Combined sources3
Beta strandi309 – 317Combined sources9
Helixi318 – 320Combined sources3
Turni328 – 330Combined sources3
Beta strandi336 – 342Combined sources7
Beta strandi364 – 369Combined sources6
Beta strandi372 – 380Combined sources9
Beta strandi1118 – 1123Combined sources6
Helixi1436 – 1454Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XIPX-ray2.50A2-387[»]
3PBPX-ray2.60C/F/I/L1425-1460[»]
3RRMX-ray2.88C2-387[»]
3TKNX-ray3.40B/E/H1425-1460[»]
4DS1X-ray1.85B/D1116-1126[»]
ProteinModelPortaliP40477.
SMRiP40477.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40477.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati228 – 231FG 14
Repeati267 – 270PXFG 14
Repeati462 – 470SXFGXPXFG 19
Repeati503 – 511SXFGXPXFG 2; approximate9
Repeati522 – 530SXFGXPXFG 3; approximate9
Repeati532 – 535PXFG 24
Repeati548 – 556SXFGXPXFG 49
Repeati558 – 561PXFG 34
Repeati574 – 582SXFGXPXFG 59
Repeati584 – 587PXFG 44
Repeati600 – 608SXFGXPXFG 69
Repeati610 – 613SXFG 14
Repeati624 – 632SXFGXPXFG 7; approximate9
Repeati642 – 645FG 24
Repeati687 – 690FG 34
Repeati704 – 707FXFG 14
Repeati709 – 712SXFG 24
Repeati728 – 731FXFG 24
Repeati842 – 845PXFG 54
Repeati873 – 876FXFG 34

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 500Interaction with DBP5Add BLAST500
Regioni497 – 701Interactions with CRM1 and GLE1Add BLAST205
Regioni1223 – 1460Interaction with NUP821 PublicationAdd BLAST238

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1279 – 1320Add BLAST42
Coiled coili1383 – 1418Add BLAST36

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi455 – 766Ser-richAdd BLAST312

Domaini

Contains FG repeats. FG repeats are interaction sites for karyopherins (importins, exportins) and form probably an affinity gradient, guiding the transport proteins unidirectionally with their cargo through the NPC. FG repeat regions are highly flexible and lack ordered secondary structure. The overall conservation of FG repeats regarding exact sequence, spacing, and repeat unit length is limited. FG repeat types and their physico-chemical environment change across the NPC from the nucleoplasmic to the cytoplasmic side: SXFG/PXFG repeats are especially abundant in NUPs on the cytoplasmic side.

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

GeneTreeiENSGT00730000113975.
HOGENOMiHOG000113875.
InParanoidiP40477.
KOiK18715.
OMAiFGESAFG.
OrthoDBiEOG092C0CCA.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40477-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLKDEVPT ETSEDFGFKF LGQKQILPSF NEKLPFASLQ NLDISNSKSL
60 70 80 90 100
FVAASGSKAV VGELQLLRDH ITSDSTPLTF KWEKEIPDVI FVCFHGDQVL
110 120 130 140 150
VSTRNALYSL DLEELSEFRT VTSFEKPVFQ LKNVNNTLVI LNSVNDLSAL
160 170 180 190 200
DLRTKSTKQL AQNVTSFDVT NSQLAVLLKD RSFQSFAWRN GEMEKQFEFS
210 220 230 240 250
LPSELEELPV EEYSPLSVTI LSPQDFLAVF GNVISETDDE VSYDQKMYII
260 270 280 290 300
KHIDGSASFQ ETFDITPPFG QIVRFPYMYK VTLSGLIEPD ANVNVLASSC
310 320 330 340 350
SSEVSIWDSK QVIEPSQDSE RAVLPISEET DKDTNPIGVA VDVVTSGTIL
360 370 380 390 400
EPCSGVDTIE RLPLVYILNN EGSLQIVGLF HVAAIKSGHY SINLESLEHE
410 420 430 440 450
KSLSPTSEKI PIAGQEQEEK KKNNESSKAL SENPFTSANT SGFTFLKTQP
460 470 480 490 500
AAANSLQSQS SSTFGAPSFG SSAFKIDLPS VSSTSTGVAS SEQDATDPAS
510 520 530 540 550
AKPVFGKPAF GAIAKEPSTS EYAFGKPSFG APSFGSGKSS VESPASGSAF
560 570 580 590 600
GKPSFGTPSF GSGNSSVEPP ASGSAFGKPS FGTPSFGSGN SSAEPPASGS
610 620 630 640 650
AFGKPSFGTS AFGTASSNET NSGSIFGKAA FGSSSFAPAN NELFGSNFTI
660 670 680 690 700
SKPTVDSPKE VDSTSPFPSS GDQSEDESKS DVDSSSTPFG TKPNTSTKPK
710 720 730 740 750
TNAFDFGSSS FGSGFSKALE SVGSDTTFKF GTQASPFSSQ LGNKSPFSSF
760 770 780 790 800
TKDDTENGSL SKGSTSEIND DNEEHESNGP NVSGNDLTDS TVEQTSSTRL
810 820 830 840 850
PETPSDEDGE VVEEEAQKSP IGKLTETIKK SANIDMAGLK NPVFGNHVKA
860 870 880 890 900
KSESPFSAFA TNITKPSSTT PAFSFGNSTM NKSNTSTVSP MEEADTKETS
910 920 930 940 950
EKGPITLKSV ENPFLPAKEE RTGESSKKDH NDDPKDGYVS GSEISVRTSE
960 970 980 990 1000
SAFDTTANEE IPKSQDVNNH EKSETDPKYS QHAVVDHDNK SKEMNETSKN
1010 1020 1030 1040 1050
NERSGQPNHG VQGDGIALKK DNEKENFDSN MAIKQFEDHQ SSEEDASEKD
1060 1070 1080 1090 1100
SRQSSEVKES DDNMSLNSDR DESISESYDK LEDINTDELP HGGEAFKARE
1110 1120 1130 1140 1150
VSASADFDVQ TSLEDNYAES GIQTDLSESS KENEVQTDAI PVKHNSTQTV
1160 1170 1180 1190 1200
KKEAVDNGLQ TEPVETCNFS VQTFEGDENY LAEQCKPKQL KEYYTSAKVS
1210 1220 1230 1240 1250
NIPFVSQNST LRLIESTFQT VEAEFTVLME NIRNMDTFFT DQSSIPLVKR
1260 1270 1280 1290 1300
TVRSINNLYT WRIPEAEILL NIQNNIKCEQ MQITNANIQD LKEKVTDYVR
1310 1320 1330 1340 1350
KDIAQITEDV ANAKEEYLFL MHFDDASSGY VKDLSTHQFR MQKTLRQKLF
1360 1370 1380 1390 1400
DVSAKINHTE ELLNILKLFT VKNKRLDDNP LVAKLAKESL ARDGLLKEIK
1410 1420 1430 1440 1450
LLREQVSRLQ LEEKGKKASS FDASSSITKD MKGFKVVEVG LAMNTKKQIG
1460
DFFKNLNMAK
Length:1,460
Mass (Da):158,908
Last modified:February 1, 1995 - v1
Checksum:iA33FFA52378F8205
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L40634 Genomic DNA. Translation: AAC41652.1.
Z38125 Genomic DNA. Translation: CAA86265.1.
BK006942 Genomic DNA. Translation: DAA08438.1.
PIRiS48457.
RefSeqiNP_012151.1. NM_001179463.1.

Genome annotation databases

EnsemblFungiiYIL115C; YIL115C; YIL115C.
GeneIDi854691.
KEGGisce:YIL115C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L40634 Genomic DNA. Translation: AAC41652.1.
Z38125 Genomic DNA. Translation: CAA86265.1.
BK006942 Genomic DNA. Translation: DAA08438.1.
PIRiS48457.
RefSeqiNP_012151.1. NM_001179463.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XIPX-ray2.50A2-387[»]
3PBPX-ray2.60C/F/I/L1425-1460[»]
3RRMX-ray2.88C2-387[»]
3TKNX-ray3.40B/E/H1425-1460[»]
4DS1X-ray1.85B/D1116-1126[»]
ProteinModelPortaliP40477.
SMRiP40477.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34876. 92 interactors.
DIPiDIP-2314N.
IntActiP40477. 17 interactors.
MINTiMINT-629459.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

PTM databases

iPTMnetiP40477.

Proteomic databases

MaxQBiP40477.
PRIDEiP40477.

Protocols and materials databases

DNASUi854691.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL115C; YIL115C; YIL115C.
GeneIDi854691.
KEGGisce:YIL115C.

Organism-specific databases

EuPathDBiFungiDB:YIL115C.
SGDiS000001377. NUP159.

Phylogenomic databases

GeneTreeiENSGT00730000113975.
HOGENOMiHOG000113875.
InParanoidiP40477.
KOiK18715.
OMAiFGESAFG.
OrthoDBiEOG092C0CCA.

Enzyme and pathway databases

BioCyciYEAST:G3O-31369-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP40477.
PROiP40477.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNU159_YEAST
AccessioniPrimary (citable) accession number: P40477
Secondary accession number(s): D6VVH2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 30, 2016
This is version 159 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1230 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.