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Protein

NADPH-dependent 1-acyldihydroxyacetone phosphate reductase

Gene

AYR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Can convert acyl and alkyl dihydroxyacetone-phosphate (DHAP) into glycerolipids and ether lipids, respectively. Required for the biosynthesis of phosphatidic acid via the DHAP pathway, where it reduces 1-acyl DHAP to lysophosphatidic acid (LPA). Required for spore germination.1 Publication

Miscellaneous

Present with 3671 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

1-palmitoylglycerol 3-phosphate + NADP+ = palmitoylglycerone phosphate + NADPH.

Enzyme regulationi

Inhibited by divalent cations and N-ethylmaleimide. Activity is reduced under anaerobic growth conditions.1 Publication

Kineticsi

  1. KM=20 µM for NADPH1 Publication
  2. KM=15 µM for hexadecyl dihydroxyacetone-phosphate1 Publication
  1. Vmax=3.8 nmol/min/mg enzyme for hexadecyl dihydroxyacetone-phosphate1 Publication

pH dependencei

Optimum pH is 6.7-7.2.1 Publication

Temperature dependencei

Optimum temperature is 40 degrees Celsius. Thermostable for 10 min up to 45 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei144SubstrateBy similarity1
Active sitei157Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 37NAD or NADPBy similarityAdd BLAST25

GO - Molecular functioni

  • 1-acyl dihydroxyacetone phosphate reductase activity Source: UniProtKB-EC
  • acylglycerone-phosphate reductase activity Source: SGD
  • triglyceride lipase activity Source: SGD

GO - Biological processi

  • phosphatidic acid biosynthetic process Source: SGD
  • triglyceride catabolic process Source: SGD

Keywordsi

Molecular functionOxidoreductase
LigandNAD, NADP

Enzyme and pathway databases

BioCyciMetaCyc:YIL124W-MONOMER
YEAST:YIL124W-MONOMER
SABIO-RKiP40471

Chemistry databases

SwissLipidsiSLP:000000056

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH-dependent 1-acyldihydroxyacetone phosphate reductase (EC:1.1.1.101)
Short name:
ADR
Alternative name(s):
1-acyl DHAP reductase
Acyl/alkyl DHAP reductase
Acylglycerone-phosphate reductase
Gene namesi
Name:AYR1
Ordered Locus Names:YIL124W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL124W
SGDiS000001386 AYR1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum, Lipid droplet

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000545201 – 297NADPH-dependent 1-acyldihydroxyacetone phosphate reductaseAdd BLAST297

Proteomic databases

MaxQBiP40471
PaxDbiP40471
PRIDEiP40471

PTM databases

iPTMnetiP40471

Interactioni

Protein-protein interaction databases

BioGridi34867, 133 interactors
DIPiDIP-4614N
IntActiP40471, 11 interactors
MINTiP40471
STRINGi4932.YIL124W

Structurei

3D structure databases

ProteinModelPortaliP40471
SMRiP40471
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

InParanoidiP40471
KOiK06123
OMAiNYTVPAM
OrthoDBiEOG092C4WZU

Family and domain databases

InterProiView protein in InterPro
IPR036291 NAD(P)-bd_dom_sf
IPR020904 Sc_DH/Rdtase_CS
IPR002347 SDR_fam
PfamiView protein in Pfam
PF00106 adh_short, 1 hit
PRINTSiPR00081 GDHRDH
PR00080 SDRFAMILY
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00061 ADH_SHORT, 1 hit

Sequencei

Sequence statusi: Complete.

P40471-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSELQSQPKK IAVVTGASGG IGYEVTKELA RNGYLVYACA RRLEPMAQLA
60 70 80 90 100
IQFGNDSIKP YKLDISKPEE IVTFSGFLRA NLPDGKLDLL YNNAGQSCTF
110 120 130 140 150
PALDATDAAV EQCFKVNVFG HINMCRELSE FLIKAKGTIV FTGSLAGVVS
160 170 180 190 200
FPFGSIYSAS KAAIHQYARG LHLEMKPFNV RVINAITGGV ATDIADKRPL
210 220 230 240 250
PETSIYNFPE GREAFNSRKT MAKDNKPMPA DAYAKQLVKD ILSTSDPVDV
260 270 280 290
YRGTFANIMR FVMIFVPYWL LEKGLSKKFK LDKVNNALKS KQKNKDD
Length:297
Mass (Da):32,814
Last modified:February 1, 1995 - v1
Checksum:iB614C0E0B1FB0CE0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z46833 Genomic DNA Translation: CAA86868.1
BK006942 Genomic DNA Translation: DAA08429.1
PIRiS49885
RefSeqiNP_012142.3, NM_001179472.3

Genome annotation databases

EnsemblFungiiYIL124W; YIL124W; YIL124W
GeneIDi854682
KEGGisce:YIL124W

Similar proteinsi

Entry informationi

Entry nameiAYR1_YEAST
AccessioniPrimary (citable) accession number: P40471
Secondary accession number(s): D6VVG3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

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