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Protein

DNA repair/transcription protein MET18/MMS19

Gene

MET18

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Key component of the cytosolic iron-sulfur protein assembly (CIA) machinery that mediates the incorporation of iron-sulfur cluster into apoproteins specifically involved in DNA metabolism and genomic integrity. Acts as an adapter between early-acting CIA components and a subset of cellular target iron-sulfur proteins such as RAD3/XPD and DNA2, thereby playing a key role in nucleotide excision repair (NER) and RNA polymerase II (POL II) transcription.5 Publications

Miscellaneous

Present with 3150 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • DNA metabolic process Source: UniProtKB
  • DNA repair Source: UniProtKB-KW
  • iron-sulfur cluster assembly Source: UniProtKB
  • protein maturation by iron-sulfur cluster transfer Source: SGD
  • response to organic substance Source: UniProtKB
  • response to UV Source: UniProtKB
  • small molecule metabolic process Source: Reactome
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Biological processDNA damage, DNA repair, Transcription

Enzyme and pathway databases

BioCyciYEAST:G3O-31379-MONOMER.
ReactomeiR-SCE-2564818. Cytosolic iron-sulfur cluster assembly (yeast).
R-SCE-2564830. Cytosolic iron-sulfur cluster assembly.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair/transcription protein MET18/MMS19
Alternative name(s):
Methyl methanesulfonate-sensitivity protein 19
Gene namesi
Name:MET18
Synonyms:MMS19
Ordered Locus Names:YIL128W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL128W.
SGDiS000001390. MET18.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000964451 – 1032DNA repair/transcription protein MET18/MMS19Add BLAST1032

Proteomic databases

MaxQBiP40469.
PRIDEiP40469.

PTM databases

iPTMnetiP40469.

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi34863. 277 interactors.
DIPiDIP-1438N.
IntActiP40469. 44 interactors.
MINTiMINT-391809.
STRINGi4932.YIL128W.

Structurei

3D structure databases

ProteinModelPortaliP40469.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati40 – 77HEAT 1Add BLAST38
Repeati121 – 160HEAT 2Add BLAST40
Repeati256 – 293HEAT 3Add BLAST38
Repeati463 – 500HEAT 4Add BLAST38
Repeati504 – 542HEAT 5Add BLAST39
Repeati550 – 587HEAT 6Add BLAST38
Repeati614 – 651HEAT 7Add BLAST38
Repeati759 – 796HEAT 8Add BLAST38
Repeati808 – 850HEAT 9Add BLAST43
Repeati863 – 901HEAT 10Add BLAST39
Repeati906 – 943HEAT 11Add BLAST38
Repeati947 – 987HEAT 12Add BLAST41
Repeati992 – 1029HEAT 13Add BLAST38

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi628 – 635Poly-Asn8

Sequence similaritiesi

Belongs to the MET18/MMS19 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00390000015583.
HOGENOMiHOG000113568.
InParanoidiP40469.
KOiK15075.
OMAiPESCWYF.
OrthoDBiEOG092C0T75.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiView protein in InterPro
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR024687. MMS19_C.
PfamiView protein in Pfam
PF12460. MMS19_C. 1 hit.
SUPFAMiSSF48371. SSF48371. 6 hits.

Sequencei

Sequence statusi: Complete.

P40469-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPDELNSAV VTFMANLNID DSKANETAST VTDSIVHRSI KLLEVVVALK
60 70 80 90 100
DYFLSENEVE RKKALTCLTT ILAKTPKDHL SKNECSVIFQ FYQSKLDDQA
110 120 130 140 150
LAKEVLEGFA ALAPMKYVSI NEIAQLLRLL LDNYQQGQHL ASTRLWPFKI
160 170 180 190 200
LRKIFDRFFV NGSSTEQVKR INDLFIETFL HVANGEKDPR NLLLSFALNK
210 220 230 240 250
SITSSLQNVE NFKEDLFDVL FCYFPITFKP PKHDPYKISN QDLKTALRSA
260 270 280 290 300
ITATPLFAED AYSNLLDKLT ASSPVVKNDT LLTLLECVRK FGGSSILENW
310 320 330 340 350
TLLWNALKFE IMQNSEGNEN TLLNPYNKDQ QSDDVGQYTN YDACLKIINL
360 370 380 390 400
MALQLYNFDK VSFEKFFTHV LDELKPNFKY EKDLKQTCQI LSAIGSGNVE
410 420 430 440 450
IFNKVISSTF PLFLINTSEV AKLKLLIMNF SFFVDSYIDL FGRTSKESLG
460 470 480 490 500
TPVPNNKMAE YKDEIIMILS MALTRSSKAE VTIRTLSVIQ FTKMIKMKGF
510 520 530 540 550
LTPEEVSLII QYFTEEILTD NNKNIYYACL EGLKTISEIY EDLVFEISLK
560 570 580 590 600
KLLDLLPDCF EEKIRVNDEE NIHIETILKI ILDFTTSRHI LVKESITFLA
610 620 630 640 650
TKLNRVAKIS KSREYCFLLI STIYSLFNNN NQNENVLNEE DALALKNAIE
660 670 680 690 700
PKLFEIITQE SAIVSDNYNL TLLSNVLFFT NLKIPQAAHQ EELDRYNELF
710 720 730 740 750
ISEGKIRILD TPNVLAISYA KILSALNKNC QFPQKFTVLF GTVQLLKKHA
760 770 780 790 800
PRMTETEKLG YLELLLVLSN KFVSEKDVIG LFDWKDLSVI NLEVMVWLTK
810 820 830 840 850
GLIMQNSLES SEIAKKFIDL LSNEEIGSLV SKLFEVFVMD ISSLKKFKGI
860 870 880 890 900
SWNNNVKILY KQKFFGDIFQ TLVSNYKNTV DMTIKCNYLT ALSLVLKHTP
910 920 930 940 950
SQSVGPFIND LFPLLLQALD MPDPEVRVSA LETLKDTTDK HHTLITEHVS
960 970 980 990 1000
TIVPLLLSLS LPHKYNSVSV RLIALQLLEM ITTVVPLNYC LSYQDDVLSA
1010 1020 1030
LIPVLSDKKR IIRKQCVDTR QVYYELGQIP FE
Length:1,032
Mass (Da):117,883
Last modified:February 1, 1995 - v1
Checksum:iD05DCC488E098814
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti230P → Q in AAB38865 (PubMed:8943333).Curated1
Sequence conflicti329D → G in AAB38865 (PubMed:8943333).Curated1
Sequence conflicti335V → M in AAB38865 (PubMed:8943333).Curated1
Sequence conflicti361V → I in AAB38865 (PubMed:8943333).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti111 – 112AL → SF in strain: SK1. 2
Natural varianti329D → G in strain: SK1. 1 Publication1
Natural varianti335V → M in strain: SK1. 1 Publication1
Natural varianti444T → I in strain: SK1. 1 Publication1
Natural varianti697N → S in strain: SK1. 1 Publication1
Natural varianti814A → S in strain: SK1. 1 Publication1
Natural varianti816K → M in strain: SK1. 1
Natural varianti930A → T in strain: SK1. 1
Natural varianti963H → Q in strain: SK1. 1
Natural varianti969S → C in strain: SK1. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U70559 Genomic DNA. Translation: AAB38865.1.
DQ115392 Genomic DNA. Translation: AAZ22499.1.
Z38059 Genomic DNA. Translation: CAA86150.1.
Z46833 Genomic DNA. Translation: CAA86864.1.
BK006942 Genomic DNA. Translation: DAA08425.1.
PIRiS53571.
RefSeqiNP_012138.1. NM_001179476.1.

Genome annotation databases

EnsemblFungiiYIL128W; YIL128W; YIL128W.
GeneIDi854678.
KEGGisce:YIL128W.

Similar proteinsi

Entry informationi

Entry nameiMET18_YEAST
AccessioniPrimary (citable) accession number: P40469
Secondary accession number(s): D6VVF9, P89106, Q45U11
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 27, 2017
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names