Reviewed, UniProtKB/Swiss-Prot P40468 (TAO3_YEAST)

Names and origin

Protein names

Recommended name:
    Cell morphogenesis protein PAG1
Alternative name(s):
    Protein TAO3

Gene names

Name:	TAO3
Synonyms:	PAG1
Ordered Locus Names:	YIL129C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	2376 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Seems to play a role in cell morphogenesis. Ref.3
Subunit structure	Associates with CBK1.
Miscellaneous	Present with 206 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	To S.pombe mor2.

Ontologies

Keywords
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell budding Ref.3 Inferred from genetic interaction. Source: SGD cell morphogenesis during vegetative growth Inferred from genetic interaction. Source: SGD
Cellular component	cellular bud Inferred from direct assay. Source: SGD incipient cellular bud site Ref.3 Inferred from direct assay. Source: SGD mating projection tip Inferred from direct assay. Source: SGD mitochondrion Inferred from direct assay. Source: SGD
Molecular function	protein binding Ref.3 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q04545	1	EBI-18961,EBI-27891
CBK1	P53894	1	EBI-18961,EBI-4110

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2376	2376	Cell morphogenesis protein PAG1		PRO_0000072430
Amino acid modifications
	Modified residue	141	1	Phosphoserine Ref.8
	Modified residue	300	1	Phosphoserine Ref.8
	Modified residue	985	1	Phosphoserine Ref.7
	Modified residue	2264	1	Phosphothreonine Ref.8 Ref.5
	Modified residue	2267	1	Phosphoserine Ref.8 Ref.5
	Modified residue	2316	1	Phosphoserine Ref.8
	Modified residue	2352	1	Phosphoserine Ref.6
	Modified residue	2355	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P40468-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 08958191BE969C1B	FASTA	2,376	269,858
	10 20 30 40 50 60 MASRFTFPPQ RDQGIGFTFP PTNKAEGSSN NNQISIDIDP SGQDVLEEIN EAPLNTFPLH 70 80 90 100 110 120 QSVTDAPIID IPSPTDMSEG TSLNNQLLLR QQQQQGTGEG QALPPTFVEE QSDQNKISML 130 140 150 160 170 180 LPEQKQQRMQ ESAPPDITAK SVAEDYVTTL RQQMATDWKS PSEYALHILF TKFIRYAENK 190 200 210 220 230 240 LNMCLQQLDM AEPPIVEILG EGVDPSFDEI IKSLGHIAKK KPKPVIDAMM FWRKTKSEAA 250 260 270 280 290 300 NSASEEMEKL LKEYEFEKAH PSQAHFLMNR RLSRSSSNTT SKYKHNNNTN NLPGMKRHVS 310 320 330 340 350 360 SSFNNKVPLI KASSSNNSAT SSPSIANSQL KSLENTIEVA KEEAFLADRK SLISIYILCR 370 380 390 400 410 420 VLNEIVKQAS SNEEEDLSDK LEEIVFTQLK TTDPLSISTS LIKSSNWNSF AELLGSMSEK 430 440 450 460 470 480 KFLSVSDRFI ADLEKIPAYI PPELEPSTHL LILGMRYLKL RNYPLEKFEE SADFMKSLSK 490 500 510 520 530 540 FFAKTENFPV CLAYAEVTNQ LLLPLAGSLT AEVNHPTWVE AMSTLLNTAK RLQADSKYWV 550 560 570 580 590 600 SGFKLTVSIL CASPPDLFSK QWLSLLEANA SKVKSKSLNE RIIFAVGLSR LVWVYLYRCP 610 620 630 640 650 660 ETLNNTTRTL TKLLQLYLNT RKKENWITGD FGLLNPLTDA LISIGFLHPN FLMEQALIPL 670 680 690 700 710 720 IRQSFNGSNL ENINYEKLIL TINTYKGLLV TKERPRFPED DNRLYELNLN NITVNQVQEA 730 740 750 760 770 780 SSINHTEISD YFYKLFLLLD SSIGSEVWSP ENQHQKQSSN AFSPFGFSFS NDNDSSKNKS 790 800 810 820 830 840 LYVILFGTII EAIPCCLSIS RTIPYKSTIE ILSRNAVHSE VIISSSSQNA LRALASKKNP 850 860 870 880 890 900 YTLITWFAKY SFDFDEKTQS SYNMSYLSSK EYNRLLILYV ELLECWLEEF QSSNKEENKK 910 920 930 940 950 960 ETGLDGIRLL PIDAEQEESN ETEKLEWKNT VTVIEEVEGN GLFFLCSHDA KIRRLGIQIL 970 980 990 1000 1010 1020 RIIFKFDEAM MEKTEKLSNG HSRSSSHFAA DRGTRLIDLL NECNTTTLIN PHKATLSAVE 1030 1040 1050 1060 1070 1080 KTRFSRLNSK YKRGLLIKLA ESEYGVDAAL WQRAFPKLLA LVFKTCPMAM ALCRSIVCIR 1090 1100 1110 1120 1130 1140 LVQVHEIILR VANDVDFKLK NVLPETIVNQ WKLYLIAACT SLTSTFDQKL HIPSNIPQHG 1150 1160 1170 1180 1190 1200 RKKSQQIFTV QHQKIKSAKS IFKMVLPLLN AKYIMIRDAI ITGLSSMNIN IFKAYVEAID 1210 1220 1230 1240 1250 1260 VFLVAWKEGS SNNQIRVEMF HILTILSPYL KSDMIFNDEW ILRKLSEFLQ KTKQFLEKDS 1270 1280 1290 1300 1310 1320 VQISYEYQSL RSYFAGLILS YYMAVREHPL IDELFPFQAR ASCFNFLKEW CGYGEYEPIS 1330 1340 1350 1360 1370 1380 EERYAIMIKN TESGRDRTAI TTGIEFQKNR LQMIVLETMV VLCSDPITQT LDDDLELPIV 1390 1400 1410 1420 1430 1440 ISFDTEDLLA WIEALFDSDN TTVKNLGVRA LENLLDKNRE NFKLFRDVAF QCVSHHSHPS 1450 1460 1470 1480 1490 1500 VAVLYYTTLC KSVLKLDNLV LDEDELVSLG LYGLVADKED TRTFAVDLLS AVETKLHNSS 1510 1520 1530 1540 1550 1560 YTKVFKERLA NSSKTVYKST AKEISSIFAE LLSQDLCLRI FSSLVRILDL FPFEIKRDLL 1570 1580 1590 1600 1610 1620 VLMVPWVNKF TLKSLEELDT FMVLNNLFYI TIDLNDSLPN EVEQLWISLG KGNSFQNIHV 1630 1640 1650 1660 1670 1680 SLEYIINSSM NHCNPLFVQY ARDIVLYLAN IPGGIGLLDT LLNNLEPKYM VPLAKHTFNE 1690 1700 1710 1720 1730 1740 PMNNNKYSFL GNIWERLNYN GKRIIFSKAQ LSIIFLVNLL TNLSESVKAK IPLLLHMSIC 1750 1760 1770 1780 1790 1800 LLDHYVPLIH ESACKIASTL IFGLAPSHEK SEETVKLLRN KHALWSYDNL MKKGARSPKT 1810 1820 1830 1840 1850 1860 MDLLIRNIIS IFSDLDEFQV TWQRIALKWA TTCSVRHIAC RSFQIFRSLL TFLDQEMLRD 1870 1880 1890 1900 1910 1920 MLHRLSNTIS DGNVDIQGFA MQILMTLNAI MAELDPTNLI SFPQLFWSIT ACLSSIHEQE 1930 1940 1950 1960 1970 1980 FIEVLSCLSK FISKIDLDSP DTVQCLVAIF PSNWEGRFDG LQQIVMTGLR SANSLEITWK 1990 2000 2010 2020 2030 2040 FLDKLNLLKD SRIIANTESR LLFALIANLP RFLNAMDRKD FTGIQVAADS LIELANAYKQ 2050 2060 2070 2080 2090 2100 PSLSRLIDSL AKNKFRSKKD FMSQVVSFIS RNYFPSYSAQ TLVFLLGLLF NKIGWIRVQT 2110 2120 2130 2140 2150 2160 LEILKYVFPL IDLRRPEFIG VGADLISPLL RLLFTEYEAK ALEVLDCVPN VSGSKMDKDV 2170 2180 2190 2200 2210 2220 LRITMGNKDV KDGDNATTTL FGLPEDSGWS VPMPTMTAAT TRHNVHAVFM TCGTGKSDEV 2230 2240 2250 2260 2270 2280 SAHGSDDMDA VIEFHADGDY ELGRMDTIVE FHADGDYDLG RMDTNDSISV AEEKDASLSH 2290 2300 2310 2320 2330 2340 MWAELDNLDS FFTKDTNVPN ISSKMGMGIP HGRSDSIETT RTDQTFSFES APQLYDKKVS 2350 2360 2370 VILNRSLSRT PSNVSFKTHL ADSFAVKINR NGKPRI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Quantitative trait loci mapped to single-nucleotide resolution in yeast." Deutschbauer A.M., Davis R.W. Nat. Genet. 37:1333-1340(2005) [PubMed: 16273108] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 200060 / W303, ATCC 204508 / S288c, ATCC 204626 / S288c / A364A and Sigma 1278B.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed: 9169870] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Pag1p, a novel protein associated with protein kinase Cbk1p, is required for cell morphogenesis and proliferation in Saccharomyces cerevisiae." Du L.L., Novick P. Mol. Biol. Cell 13:503-514(2002) [PubMed: 11854408] [Abstract] Cited for: FUNCTION, INTERACTION WITH CBK1.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-2264 AND SER-2267, MASS SPECTROMETRY.
[6]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-2352 AND SER-2355, MASS SPECTROMETRY.
[7]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-985, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-141; SER-300; THR-2264; SER-2267 AND SER-2316, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ116812 Genomic DNA. Translation: AAZ23264.1. DQ116814 Genomic DNA. Translation: AAZ23266.1. DQ116817 Genomic DNA. Translation: AAZ23269.1. DQ116824 Genomic DNA. Translation: AAZ23276.1. Z38059 Genomic DNA. Translation: CAA86149.1.
PIR	S48405.
RefSeq	NP_012137.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5950N.
IntAct	P40468. 19 interactions.
STRING	P40468.
Proteomic databases
PRIDE	P40468.
Genome annotation databases
Ensembl	YIL129C; YIL129C; YIL129C; Saccharomyces cerevisiae. [Genome view]
GeneID	854677.
KEGG	sce:YIL129C.
NMPDR	fig|4932.3.peg.1661.
Organism-specific databases
CYGD	YIL129c.
SGD	S000001391. TAO3.
Phylogenomic databases
HOGENOM	P40468.
OMA	IAYENDE
OrthoDB	EOG9283HZ
Gene expression databases
ArrayExpress	P40468.
Genevestigator	P40468.
GermOnline	YIL129C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR016024. ARM-type_fold. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	977277.

Entry information

Entry name

TAO3_YEAST

Accession

Primary (citable) accession number: P40468
Secondary accession number(s): Q45T93

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 24, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)