Kinetochore protein NDC80 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P40460 (NDC80_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 104. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Kinetochore protein NDC80

Alternative name(s):
Protein TID3

Gene names

Name:	TID3
Synonyms:	HEC1, NDC80
Ordered Locus Names:	YIL144W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	691 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity. Ref.4 Ref.5 Ref.7 Ref.10
Subunit structure	Component of the NDC80 complex, which consists of TID3/NDC80, NUF2, SPC24 and SPC25. The NDC80 complex is formed by two subcomplexes, TID3/NDC80-NUF2 and SPC24-SPC25, which are joined end-to-end through their coiled-coil domains. It has a rod-like structure with a length of 570 Angstroms and globular domains at either end. The TID3/NDC80-NUF2 globular domains are probably directed to microtubules, the SPC24-SPC25 globular domains to the centromere. TID3 probably interacts with SMC1 and SMC2. Also interacts with KIN3. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.12
Subcellular location	Nucleus. Chromosome › centromere › kinetochore. Note: Associated with kinetochores. Ref.3 Ref.4 Ref.5 Ref.6 Ref.7
Miscellaneous	Present with 1160 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the NDC80/HEC1 family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Centromere Chromosome Kinetochore Nucleus
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW chromosome segregation Inferred from mutant phenotype Ref.7. Source: SGD microtubule nucleation Inferred from physical interaction PubMed 9153752. Source: SGD mitosis Inferred from electronic annotation. Source: UniProtKB-KW protein localization to kinetochore Inferred from mutant phenotype PubMed 19893618. Source: SGD
Cellular_component	Ndc80 complex Inferred from direct assay Ref.6 Ref.7. Source: SGD condensed nuclear chromosome kinetochore Inferred from direct assay PubMed 11511347. Source: SGD
Molecular_function	structural constituent of cytoskeleton Inferred from physical interaction Ref.4. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct
itself		4	EBI-25247,EBI-25247
DAM1	P53267	4	EBI-25247,EBI-23268
NUF2	P33895	16	EBI-25247,EBI-12377
SPC105	P53148	2	EBI-25247,EBI-23870
SPC24	Q04477	17	EBI-25247,EBI-27228
SPC25	P40014	13	EBI-25247,EBI-22458

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 691

691

Kinetochore protein NDC80

PRO_0000202958

Regions

Coiled coil

376 – 446

Potential

Coiled coil

522 – 686

165

Potential

Amino acid modifications

Modified residue

248

Phosphothreonine Ref.13

Experimental info

Mutagenesis

201

S → A: Loss of function. Ref.8

Sequences

Sequence

Length

Mass (Da)

Tools

P40460 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 7DBC492227A80093
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FASTA

691

80,487

        10         20         30         40         50         60 
MQSSTSTDQH VLHHMDPHRF TSQIPTATSS QLRRRNSTNQ GLTDMINKSI ARNTISGTGI 

        70         80         90        100        110        120 
PTGGINKNKR TRSTVAGGTN GTALALNDKS NSRNSVSRLS INQLGSLQQH LSNRDPRPLR 

       130        140        150        160        170        180 
DKNFQSAIQE EIYDYLKKNK FDIETNHPIS IKFLKQPTQK GFIIIFKWLY LRLDPGYGFT 

       190        200        210        220        230        240 
KSIENEIYQI LKNLRYPFLE SINKSQISAV GGSNWHKFLG MLHWMVRTNI KLDMCLNKVD 

       250        260        270        280        290        300 
RSLINQNTQE ITILSQPLKT LDEQDQRQER YELMVEKLLI DYFTESYKSF LKLEDNYEPS 

       310        320        330        340        350        360 
MQELKLGFEK FVHIINTDIA NLQTQNDNLY EKYQEVMKIS QKIKTTREKW KALKSDSNKY 

       370        380        390        400        410        420 
ENYVNAMKQK SQEWPGKLEK MKSECELKEE EIKALQSNIS ELHKILRKKG ISTEQFELQN 

       430        440        450        460        470        480 
QEREKLTREL DKINIQSDKL TSSIKSRKLE AEGIFKSLLD TLRQYDSSIQ NLTRSRSQLG 

       490        500        510        520        530        540 
HNVNDSSLKI NISENLLDRD FHEGISYEQL FPKGSGINES IKKSILKLND EIQERIKTIE 

       550        560        570        580        590        600 
KDNITLEKDI KNLKHDINEK TQINEKLELE LSEANSKFEL SKQENERLLV AQRIEIEKME 

       610        620        630        640        650        660 
KKINDSNLLM KTKISDAEEL VTSTELKLEE LKVDLNRKRY KLHQQVIHVI DITSKFKINI 

       670        680        690 
QSSLENSENE LGNVIEELRN LEFETEHNVT N

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Components of the yeast spindle and spindle pole body." Rout M.P., Kilmartin J.V. J. Cell Biol. 111:1913-1927(1990) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[4]	"Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry." Wigge P.A., Jensen O.N., Holmes S., Soues S., Mann M., Kilmartin J.V. J. Cell Biol. 141:967-977(1998) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, SUBCELLULAR LOCATION.
[5]	"Hec1p, an evolutionarily conserved coiled-coil protein, modulates chromosome segregation through interaction with SMC proteins." Zheng L., Chen Y., Lee W.-H. Mol. Cell. Biol. 19:5417-5428(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SMC1 AND SMC2.
[6]	"The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control." Janke C., Ortiz J., Lechner J., Shevchenko A., Shevchenko A., Magiera M.M., Schramm C., Schiebel E. EMBO J. 20:777-791(2001) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SPC24 AND SPC25.
[7]	"The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation." Wigge P.A., Kilmartin J.V. J. Cell Biol. 152:349-360(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE NDC80 COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION IN THE NDC80 COMPLEX.
[8]	"Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation." Chen Y., Riley D.J., Zheng L., Chen P.-L., Lee W.-H. J. Biol. Chem. 277:49408-49416(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH KIN3, MUTAGENESIS OF SER-201.
[9]	"Spc24 interacts with Mps2 and is required for chromosome segregation, but is not implicated in spindle pole body duplication." Le Masson I., Saveanu C., Chevalier A., Namane A., Gobin R., Fromont-Racine M., Jacquier A., Mann C. Mol. Microbiol. 43:1431-1443(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SPC24 AND SPC25.
[10]	"The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity." McCleland M.L., Gardner R.D., Kallio M.J., Daum J.R., Gorbsky G.J., Burke D.J., Stukenberg P.T. Genes Dev. 17:101-114(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE NDC80 COMPLEX.
[11]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[12]	"The fission yeast kinetochore component Spc7 associates with the EB1 family member Mal3 and is required for kinetochore-spindle association." Kerres A., Vietmeier-Decker C., Ortiz J., Karig I., Beuter C., Hegemann J., Lechner J., Fleig U. Mol. Biol. Cell 15:5255-5267(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE NDC80 COMPLEX.
[13]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-248, MASS SPECTROMETRY.
[14]	"Molecular organization of the Ndc80 complex, an essential kinetochore component." Wei R.R., Sorger P.K., Harrison S.C. Proc. Natl. Acad. Sci. U.S.A. 102:5363-5367(2005) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF THE NDC80 COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z38059 Genomic DNA. Translation: CAA86134.1. BK006942 Genomic DNA. Translation: DAA08409.1.
PIR	S48390.
RefSeq	NP_012122.3. NM_001179492.3.
3D structure databases
ProteinModelPortal	P40460.
SMR	P40460. Positions 114-362.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-818N.
IntAct	P40460. 30 interactions.
MINT	MINT-408680.
STRING	4932.YIL144W.
Proteomic databases
PaxDb	P40460.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIL144W; YIL144W; YIL144W.
GeneID	854662.
KEGG	sce:YIL138C. sce:YIL144W.
Organism-specific databases
CYGD	YIL144w.
SGD	S000001406. TID3.
Phylogenomic databases
eggNOG	COG5185.
GeneTree	ENSGT00390000018386.
HOGENOM	HOG000113749.
KO	K11547.
OMA	PTQKDFN.
OrthoDB	EOG43XZBQ.
Gene expression databases
Genevestigator	P40460.
GermOnline	YIL144W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005550. Kinetochore_Ndc80. [Graphical view]
PANTHER	PTHR10643. PTHR10643. 1 hit.
Pfam	PF03801. Ndc80_HEC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977235.

Entry information

Entry name

NDC80_YEAST

Accession

Primary (citable) accession number: P40460
Secondary accession number(s): D6VVE3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 104 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents