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P40460 (NDC80_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kinetochore protein NDC80
Alternative name(s):
Protein TID3
Gene names
Name:TID3
Synonyms:HEC1, NDC80
Ordered Locus Names:YIL144W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length691 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity. Ref.4 Ref.5 Ref.7 Ref.10

Subunit structure

Component of the NDC80 complex, which consists of TID3/NDC80, NUF2, SPC24 and SPC25. The NDC80 complex is formed by two subcomplexes, TID3/NDC80-NUF2 and SPC24-SPC25, which are joined end-to-end through their coiled-coil domains. It has a rod-like structure with a length of 570 Angstroms and globular domains at either end. The TID3/NDC80-NUF2 globular domains are probably directed to microtubules, the SPC24-SPC25 globular domains to the centromere. TID3 probably interacts with SMC1 and SMC2. Also interacts with KIN3. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.12

Subcellular location

Nucleus. Chromosomecentromerekinetochore. Note: Associated with kinetochores. Ref.3 Ref.4 Ref.5 Ref.6 Ref.7

Miscellaneous

Present with 1160 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the NDC80/HEC1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 691691Kinetochore protein NDC80
PRO_0000202958

Regions

Coiled coil376 – 44671 Potential
Coiled coil522 – 686165 Potential

Amino acid modifications

Modified residue381Phosphothreonine Ref.14
Modified residue2481Phosphothreonine Ref.13

Experimental info

Mutagenesis2011S → A: Loss of function. Ref.8

Sequences

Sequence LengthMass (Da)Tools
P40460 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 7DBC492227A80093

FASTA69180,487
        10         20         30         40         50         60 
MQSSTSTDQH VLHHMDPHRF TSQIPTATSS QLRRRNSTNQ GLTDMINKSI ARNTISGTGI 

        70         80         90        100        110        120 
PTGGINKNKR TRSTVAGGTN GTALALNDKS NSRNSVSRLS INQLGSLQQH LSNRDPRPLR 

       130        140        150        160        170        180 
DKNFQSAIQE EIYDYLKKNK FDIETNHPIS IKFLKQPTQK GFIIIFKWLY LRLDPGYGFT 

       190        200        210        220        230        240 
KSIENEIYQI LKNLRYPFLE SINKSQISAV GGSNWHKFLG MLHWMVRTNI KLDMCLNKVD 

       250        260        270        280        290        300 
RSLINQNTQE ITILSQPLKT LDEQDQRQER YELMVEKLLI DYFTESYKSF LKLEDNYEPS 

       310        320        330        340        350        360 
MQELKLGFEK FVHIINTDIA NLQTQNDNLY EKYQEVMKIS QKIKTTREKW KALKSDSNKY 

       370        380        390        400        410        420 
ENYVNAMKQK SQEWPGKLEK MKSECELKEE EIKALQSNIS ELHKILRKKG ISTEQFELQN 

       430        440        450        460        470        480 
QEREKLTREL DKINIQSDKL TSSIKSRKLE AEGIFKSLLD TLRQYDSSIQ NLTRSRSQLG 

       490        500        510        520        530        540 
HNVNDSSLKI NISENLLDRD FHEGISYEQL FPKGSGINES IKKSILKLND EIQERIKTIE 

       550        560        570        580        590        600 
KDNITLEKDI KNLKHDINEK TQINEKLELE LSEANSKFEL SKQENERLLV AQRIEIEKME 

       610        620        630        640        650        660 
KKINDSNLLM KTKISDAEEL VTSTELKLEE LKVDLNRKRY KLHQQVIHVI DITSKFKINI 

       670        680        690 
QSSLENSENE LGNVIEELRN LEFETEHNVT N 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Components of the yeast spindle and spindle pole body."
Rout M.P., Kilmartin J.V.
J. Cell Biol. 111:1913-1927(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[4]"Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry."
Wigge P.A., Jensen O.N., Holmes S., Soues S., Mann M., Kilmartin J.V.
J. Cell Biol. 141:967-977(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, SUBCELLULAR LOCATION.
[5]"Hec1p, an evolutionarily conserved coiled-coil protein, modulates chromosome segregation through interaction with SMC proteins."
Zheng L., Chen Y., Lee W.-H.
Mol. Cell. Biol. 19:5417-5428(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SMC1 AND SMC2.
[6]"The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control."
Janke C., Ortiz J., Lechner J., Shevchenko A., Shevchenko A., Magiera M.M., Schramm C., Schiebel E.
EMBO J. 20:777-791(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SPC24 AND SPC25.
[7]"The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation."
Wigge P.A., Kilmartin J.V.
J. Cell Biol. 152:349-360(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE NDC80 COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION IN THE NDC80 COMPLEX.
[8]"Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation."
Chen Y., Riley D.J., Zheng L., Chen P.-L., Lee W.-H.
J. Biol. Chem. 277:49408-49416(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH KIN3, MUTAGENESIS OF SER-201.
[9]"Spc24 interacts with Mps2 and is required for chromosome segregation, but is not implicated in spindle pole body duplication."
Le Masson I., Saveanu C., Chevalier A., Namane A., Gobin R., Fromont-Racine M., Jacquier A., Mann C.
Mol. Microbiol. 43:1431-1443(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SPC24 AND SPC25.
[10]"The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity."
McCleland M.L., Gardner R.D., Kallio M.J., Daum J.R., Gorbsky G.J., Burke D.J., Stukenberg P.T.
Genes Dev. 17:101-114(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE NDC80 COMPLEX.
[11]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[12]"The fission yeast kinetochore component Spc7 associates with the EB1 family member Mal3 and is required for kinetochore-spindle association."
Kerres A., Vietmeier-Decker C., Ortiz J., Karig I., Beuter C., Hegemann J., Lechner J., Fleig U.
Mol. Biol. Cell 15:5255-5267(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE NDC80 COMPLEX.
[13]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-248, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-38, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[15]"Molecular organization of the Ndc80 complex, an essential kinetochore component."
Wei R.R., Sorger P.K., Harrison S.C.
Proc. Natl. Acad. Sci. U.S.A. 102:5363-5367(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING OF THE NDC80 COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z38059 Genomic DNA. Translation: CAA86134.1.
BK006942 Genomic DNA. Translation: DAA08409.1.
PIRS48390.
RefSeqNP_012122.3. NM_001179492.3.

3D structure databases

ProteinModelPortalP40460.
SMRP40460. Positions 114-362.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34848. 93 interactions.
DIPDIP-818N.
IntActP40460. 39 interactions.
MINTMINT-408680.
STRING4932.YIL144W.

Proteomic databases

MaxQBP40460.
PaxDbP40460.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYIL144W; YIL144W; YIL144W.
GeneID854662.
KEGGsce:YIL144W.

Organism-specific databases

CYGDYIL144w.
SGDS000001406. TID3.

Phylogenomic databases

eggNOGCOG5185.
GeneTreeENSGT00390000018386.
HOGENOMHOG000113749.
KOK11547.
OMAREYEECM.
OrthoDBEOG7034SB.

Enzyme and pathway databases

BioCycYEAST:G3O-31394-MONOMER.

Gene expression databases

GenevestigatorP40460.

Family and domain databases

InterProIPR005550. Kinetochore_Ndc80.
[Graphical view]
PANTHERPTHR10643. PTHR10643. 1 hit.
PfamPF03801. Ndc80_HEC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977235.
PROP40460.

Entry information

Entry nameNDC80_YEAST
AccessionPrimary (citable) accession number: P40460
Secondary accession number(s): D6VVE3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families