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Protein

Kinetochore protein NDC80

Gene

NDC80

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.4 Publications

Miscellaneous

Present with 1160 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • structural constituent of cytoskeleton Source: SGD

GO - Biological processi

  • attachment of mitotic spindle microtubules to kinetochore Source: GO_Central
  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: SGD
  • microtubule nucleation Source: SGD
  • protein localization to kinetochore Source: SGD

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-31394-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore protein NDC801 Publication
Alternative name(s):
80 kDa spindle component protein1 Publication
Nuclear division cycle protein 801 Publication
Two-hybrid interaction with DMC1 protein 31 Publication
Gene namesi
Name:NDC801 Publication
Synonyms:HEC11 Publication, TID31 Publication
Ordered Locus Names:YIL144WImported
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL144W.
SGDiS000001406. NDC80.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi201S → A: Loss of function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002029581 – 691Kinetochore protein NDC80Add BLAST691

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei38PhosphothreonineCombined sources1
Modified residuei248PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40460.
PRIDEiP40460.

PTM databases

iPTMnetiP40460.

Interactioni

Subunit structurei

Component of the NDC80 complex, which consists of NDC80, NUF2, SPC24 and SPC25. The NDC80 complex is formed by two subcomplexes, NDC80-NUF2 and SPC24-SPC25, which are joined end-to-end through their coiled-coil domains. It has a rod-like structure with a length of 570 Angstroms and globular domains at either end. The NDC80-NUF2 globular domains are probably directed to microtubules, the SPC24-SPC25 globular domains to the centromere. NDC80 probably interacts with SMC1 and SMC2. Also interacts with KIN3. Interacts with DMC1.7 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi34848. 274 interactors.
DIPiDIP-818N.
ELMiP40460.
IntActiP40460. 42 interactors.
MINTiMINT-408680.
STRINGi4932.YIL144W.

Structurei

Secondary structure

1691
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi122 – 138Combined sources17
Helixi141 – 144Combined sources4
Helixi159 – 173Combined sources15
Helixi184 – 194Combined sources11
Helixi199 – 201Combined sources3
Helixi204 – 207Combined sources4
Helixi215 – 254Combined sources40
Turni261 – 263Combined sources3
Helixi267 – 292Combined sources26
Helixi298 – 318Combined sources21
Helixi621 – 679Combined sources59

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5TCSX-ray2.83A114-689[»]
5TD8X-ray7.53A114-691[»]
ProteinModelPortaliP40460.
SMRiP40460.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili376 – 446Sequence analysisAdd BLAST71
Coiled coili522 – 686Sequence analysisAdd BLAST165

Sequence similaritiesi

Belongs to the NDC80/HEC1 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00390000018386.
HOGENOMiHOG000113749.
InParanoidiP40460.
KOiK11547.
OMAiKSPTQKD.
OrthoDBiEOG092C1AHP.

Family and domain databases

InterProiView protein in InterPro
IPR005550. Kinetochore_Ndc80.
PfamiView protein in Pfam
PF03801. Ndc80_HEC. 1 hit.

Sequencei

Sequence statusi: Complete.

P40460-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSSTSTDQH VLHHMDPHRF TSQIPTATSS QLRRRNSTNQ GLTDMINKSI
60 70 80 90 100
ARNTISGTGI PTGGINKNKR TRSTVAGGTN GTALALNDKS NSRNSVSRLS
110 120 130 140 150
INQLGSLQQH LSNRDPRPLR DKNFQSAIQE EIYDYLKKNK FDIETNHPIS
160 170 180 190 200
IKFLKQPTQK GFIIIFKWLY LRLDPGYGFT KSIENEIYQI LKNLRYPFLE
210 220 230 240 250
SINKSQISAV GGSNWHKFLG MLHWMVRTNI KLDMCLNKVD RSLINQNTQE
260 270 280 290 300
ITILSQPLKT LDEQDQRQER YELMVEKLLI DYFTESYKSF LKLEDNYEPS
310 320 330 340 350
MQELKLGFEK FVHIINTDIA NLQTQNDNLY EKYQEVMKIS QKIKTTREKW
360 370 380 390 400
KALKSDSNKY ENYVNAMKQK SQEWPGKLEK MKSECELKEE EIKALQSNIS
410 420 430 440 450
ELHKILRKKG ISTEQFELQN QEREKLTREL DKINIQSDKL TSSIKSRKLE
460 470 480 490 500
AEGIFKSLLD TLRQYDSSIQ NLTRSRSQLG HNVNDSSLKI NISENLLDRD
510 520 530 540 550
FHEGISYEQL FPKGSGINES IKKSILKLND EIQERIKTIE KDNITLEKDI
560 570 580 590 600
KNLKHDINEK TQINEKLELE LSEANSKFEL SKQENERLLV AQRIEIEKME
610 620 630 640 650
KKINDSNLLM KTKISDAEEL VTSTELKLEE LKVDLNRKRY KLHQQVIHVI
660 670 680 690
DITSKFKINI QSSLENSENE LGNVIEELRN LEFETEHNVT N
Length:691
Mass (Da):80,487
Last modified:February 1, 1995 - v1
Checksum:i7DBC492227A80093
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38059 Genomic DNA. Translation: CAA86134.1.
BK006942 Genomic DNA. Translation: DAA08409.1.
PIRiS48390.
RefSeqiNP_012122.3. NM_001179492.3.

Genome annotation databases

EnsemblFungiiYIL144W; YIL144W; YIL144W.
GeneIDi854662.
KEGGisce:YIL144W.

Similar proteinsi

Entry informationi

Entry nameiNDC80_YEAST
AccessioniPrimary (citable) accession number: P40460
Secondary accession number(s): D6VVE3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 25, 2017
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names