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P40460

- NDC80_YEAST

UniProt

P40460 - NDC80_YEAST

Protein

Kinetochore protein NDC80

Gene

TID3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.4 Publications

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. protein binding Source: IntAct
    3. structural constituent of cytoskeleton Source: SGD

    GO - Biological processi

    1. chromosome segregation Source: SGD
    2. microtubule nucleation Source: SGD
    3. mitotic nuclear division Source: UniProtKB-KW
    4. protein localization to kinetochore Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31394-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kinetochore protein NDC80
    Alternative name(s):
    Protein TID3
    Gene namesi
    Name:TID3
    Synonyms:HEC1, NDC80
    Ordered Locus Names:YIL144W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    CYGDiYIL144w.
    SGDiS000001406. TID3.

    Subcellular locationi

    Nucleus. Chromosomecentromerekinetochore
    Note: Associated with kinetochores.

    GO - Cellular componenti

    1. condensed nuclear chromosome, centromeric region Source: SGD
    2. condensed nuclear chromosome kinetochore Source: SGD
    3. Ndc80 complex Source: SGD

    Keywords - Cellular componenti

    Centromere, Chromosome, Kinetochore, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi201 – 2011S → A: Loss of function. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 691691Kinetochore protein NDC80PRO_0000202958Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei38 – 381Phosphothreonine1 Publication
    Modified residuei248 – 2481Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP40460.
    PaxDbiP40460.

    Expressioni

    Gene expression databases

    GenevestigatoriP40460.

    Interactioni

    Subunit structurei

    Component of the NDC80 complex, which consists of TID3/NDC80, NUF2, SPC24 and SPC25. The NDC80 complex is formed by two subcomplexes, TID3/NDC80-NUF2 and SPC24-SPC25, which are joined end-to-end through their coiled-coil domains. It has a rod-like structure with a length of 570 Angstroms and globular domains at either end. The TID3/NDC80-NUF2 globular domains are probably directed to microtubules, the SPC24-SPC25 globular domains to the centromere. TID3 probably interacts with SMC1 and SMC2. Also interacts with KIN3.6 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself4EBI-25247,EBI-25247
    DAM1P532674EBI-25247,EBI-23268
    MPS1P541994EBI-25247,EBI-11224
    NUF2P3389516EBI-25247,EBI-12377
    SPC105P531482EBI-25247,EBI-23870
    SPC24Q0447719EBI-25247,EBI-27228
    SPC25P4001413EBI-25247,EBI-22458

    Protein-protein interaction databases

    BioGridi34848. 93 interactions.
    DIPiDIP-818N.
    IntActiP40460. 39 interactions.
    MINTiMINT-408680.
    STRINGi4932.YIL144W.

    Structurei

    3D structure databases

    ProteinModelPortaliP40460.
    SMRiP40460. Positions 114-362.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili376 – 44671Sequence AnalysisAdd
    BLAST
    Coiled coili522 – 686165Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the NDC80/HEC1 family.Curated

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5185.
    GeneTreeiENSGT00390000018386.
    HOGENOMiHOG000113749.
    KOiK11547.
    OMAiREYEECM.
    OrthoDBiEOG7034SB.

    Family and domain databases

    InterProiIPR005550. Kinetochore_Ndc80.
    [Graphical view]
    PANTHERiPTHR10643. PTHR10643. 1 hit.
    PfamiPF03801. Ndc80_HEC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40460-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQSSTSTDQH VLHHMDPHRF TSQIPTATSS QLRRRNSTNQ GLTDMINKSI    50
    ARNTISGTGI PTGGINKNKR TRSTVAGGTN GTALALNDKS NSRNSVSRLS 100
    INQLGSLQQH LSNRDPRPLR DKNFQSAIQE EIYDYLKKNK FDIETNHPIS 150
    IKFLKQPTQK GFIIIFKWLY LRLDPGYGFT KSIENEIYQI LKNLRYPFLE 200
    SINKSQISAV GGSNWHKFLG MLHWMVRTNI KLDMCLNKVD RSLINQNTQE 250
    ITILSQPLKT LDEQDQRQER YELMVEKLLI DYFTESYKSF LKLEDNYEPS 300
    MQELKLGFEK FVHIINTDIA NLQTQNDNLY EKYQEVMKIS QKIKTTREKW 350
    KALKSDSNKY ENYVNAMKQK SQEWPGKLEK MKSECELKEE EIKALQSNIS 400
    ELHKILRKKG ISTEQFELQN QEREKLTREL DKINIQSDKL TSSIKSRKLE 450
    AEGIFKSLLD TLRQYDSSIQ NLTRSRSQLG HNVNDSSLKI NISENLLDRD 500
    FHEGISYEQL FPKGSGINES IKKSILKLND EIQERIKTIE KDNITLEKDI 550
    KNLKHDINEK TQINEKLELE LSEANSKFEL SKQENERLLV AQRIEIEKME 600
    KKINDSNLLM KTKISDAEEL VTSTELKLEE LKVDLNRKRY KLHQQVIHVI 650
    DITSKFKINI QSSLENSENE LGNVIEELRN LEFETEHNVT N 691
    Length:691
    Mass (Da):80,487
    Last modified:February 1, 1995 - v1
    Checksum:i7DBC492227A80093
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z38059 Genomic DNA. Translation: CAA86134.1.
    BK006942 Genomic DNA. Translation: DAA08409.1.
    PIRiS48390.
    RefSeqiNP_012122.3. NM_001179492.3.

    Genome annotation databases

    EnsemblFungiiYIL144W; YIL144W; YIL144W.
    GeneIDi854662.
    KEGGisce:YIL144W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z38059 Genomic DNA. Translation: CAA86134.1 .
    BK006942 Genomic DNA. Translation: DAA08409.1 .
    PIRi S48390.
    RefSeqi NP_012122.3. NM_001179492.3.

    3D structure databases

    ProteinModelPortali P40460.
    SMRi P40460. Positions 114-362.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34848. 93 interactions.
    DIPi DIP-818N.
    IntActi P40460. 39 interactions.
    MINTi MINT-408680.
    STRINGi 4932.YIL144W.

    Proteomic databases

    MaxQBi P40460.
    PaxDbi P40460.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIL144W ; YIL144W ; YIL144W .
    GeneIDi 854662.
    KEGGi sce:YIL144W.

    Organism-specific databases

    CYGDi YIL144w.
    SGDi S000001406. TID3.

    Phylogenomic databases

    eggNOGi COG5185.
    GeneTreei ENSGT00390000018386.
    HOGENOMi HOG000113749.
    KOi K11547.
    OMAi REYEECM.
    OrthoDBi EOG7034SB.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31394-MONOMER.

    Miscellaneous databases

    NextBioi 977235.
    PROi P40460.

    Gene expression databases

    Genevestigatori P40460.

    Family and domain databases

    InterProi IPR005550. Kinetochore_Ndc80.
    [Graphical view ]
    PANTHERi PTHR10643. PTHR10643. 1 hit.
    Pfami PF03801. Ndc80_HEC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Components of the yeast spindle and spindle pole body."
      Rout M.P., Kilmartin J.V.
      J. Cell Biol. 111:1913-1927(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
    4. "Analysis of the Saccharomyces spindle pole by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry."
      Wigge P.A., Jensen O.N., Holmes S., Soues S., Mann M., Kilmartin J.V.
      J. Cell Biol. 141:967-977(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, SUBCELLULAR LOCATION.
    5. "Hec1p, an evolutionarily conserved coiled-coil protein, modulates chromosome segregation through interaction with SMC proteins."
      Zheng L., Chen Y., Lee W.-H.
      Mol. Cell. Biol. 19:5417-5428(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH SMC1 AND SMC2.
    6. "The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control."
      Janke C., Ortiz J., Lechner J., Shevchenko A., Shevchenko A., Magiera M.M., Schramm C., Schiebel E.
      EMBO J. 20:777-791(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INTERACTION WITH SPC24 AND SPC25.
    7. "The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation."
      Wigge P.A., Kilmartin J.V.
      J. Cell Biol. 152:349-360(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE NDC80 COMPLEX, SUBCELLULAR LOCATION, IDENTIFICATION IN THE NDC80 COMPLEX.
    8. "Phosphorylation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation."
      Chen Y., Riley D.J., Zheng L., Chen P.-L., Lee W.-H.
      J. Biol. Chem. 277:49408-49416(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH KIN3, MUTAGENESIS OF SER-201.
    9. "Spc24 interacts with Mps2 and is required for chromosome segregation, but is not implicated in spindle pole body duplication."
      Le Masson I., Saveanu C., Chevalier A., Namane A., Gobin R., Fromont-Racine M., Jacquier A., Mann C.
      Mol. Microbiol. 43:1431-1443(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SPC24 AND SPC25.
    10. "The highly conserved Ndc80 complex is required for kinetochore assembly, chromosome congression, and spindle checkpoint activity."
      McCleland M.L., Gardner R.D., Kallio M.J., Daum J.R., Gorbsky G.J., Burke D.J., Stukenberg P.T.
      Genes Dev. 17:101-114(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE NDC80 COMPLEX.
    11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    12. "The fission yeast kinetochore component Spc7 associates with the EB1 family member Mal3 and is required for kinetochore-spindle association."
      Kerres A., Vietmeier-Decker C., Ortiz J., Karig I., Beuter C., Hegemann J., Lechner J., Fleig U.
      Mol. Biol. Cell 15:5255-5267(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE NDC80 COMPLEX.
    13. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-248, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    14. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-38, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    15. "Molecular organization of the Ndc80 complex, an essential kinetochore component."
      Wei R.R., Sorger P.K., Harrison S.C.
      Proc. Natl. Acad. Sci. U.S.A. 102:5363-5367(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF THE NDC80 COMPLEX.

    Entry informationi

    Entry nameiNDC80_YEAST
    AccessioniPrimary (citable) accession number: P40460
    Secondary accession number(s): D6VVE3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1160 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3