P40459 (PANC_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pantoate--beta-alanine ligase
EC=6.3.2.1

Alternative name(s):
Pantoate-activating enzyme
Pantothenate synthetase

Gene names

Name:	PAN6
Ordered Locus Names:	YIL145C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for pantothenic acid biosynthesis. Ref.3
Catalytic activity	ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate.
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1.
Subcellular location	Cytoplasm. Nucleus Ref.5.
Miscellaneous	Present with 2400 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the pantothenate synthetase family.
Sequence caution	The sequence CAA86133.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pantothenate biosynthetic process Inferred from mutant phenotype Ref.3. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay Ref.5. Source: SGD nucleus Inferred from direct assay Ref.5. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pantoate-beta-alanine ligase activity Inferred from direct assay PubMed 10417331. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Pantoate--beta-alanine ligase		PRO_0000128298

Sequences

	Sequence		Length	Mass (Da)
	P40459 [UniParc]. Last modified December 20, 2005. Version 2. Checksum: 9787B366795B5D81	FASTA	309	35,032
	10 20 30 40 50 60 MKIFHTVEEV VQWRTQELRE TRFRETIGFV PTMGCLHSGH ASLISQSVKE NTYTVVSIFV 70 80 90 100 110 120 NPSQFAPTED LDNYPRTLPD DIKLLESLKV DVLFAPNAHV MYPQGIPLDI EEQKGPFVSV 130 140 150 160 170 180 LGLSEKLEGK TRPNFFRGVA TVVTKLFNIV MADVAYFGQK DIQQFIVLQC MVDELFVNTR 190 200 210 220 230 240 LQMMPIVRNN NGLALSSRNK YLCPESLKIS ENLYRGLKAA ENAIRRLAPG GRLSRSEIID 250 260 270 280 290 300 TVTQIWAPYV DSHDFKIDYV SLADFKTLDE LSDVENTSEQ QPIVISCAVY VTDREKPDTV VRLIDNIVI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Saccharomyces cerevisiae is capable of de novo pantothenic acid biosynthesis involving a novel pathway of beta-alanine production from spermine." White W.H., Gunyuzlu P.L., Toyn J.H. J. Biol. Chem. 276:10794-10800(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z38059 Genomic DNA. Translation: CAA86133.1. Different initiation. BK006942 Genomic DNA. Translation: DAA08408.1.
PIR	S48389.
RefSeq	NP_012121.2. NM_001179493.1.
3D structure databases
ProteinModelPortal	P40459.
SMR	P40459. Positions 1-309.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4613N.
IntAct	P40459. 2 interactions.
MINT	MINT-560279.
STRING	4932.YIL145C.
Proteomic databases
PaxDb	P40459.
PeptideAtlas	P40459.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIL145C; YIL145C; YIL145C.
GeneID	854661.
KEGG	sce:YIL145C.
Organism-specific databases
CYGD	YIL145c.
SGD	S000001407. PAN6.
Phylogenomic databases
eggNOG	COG0414.
HOGENOM	HOG000175516.
KO	K01918.
OMA	SQFAPHE.
OrthoDB	EOG43JGDR.
Enzyme and pathway databases
UniPathway	UPA00028; UER00005.
Gene expression databases
Genevestigator	P40459.
GermOnline	YIL145C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
InterPro	IPR003721. Pantoate_ligase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR21299:SF1. PTHR21299:SF1. 1 hit.
Pfam	PF02569. Pantoate_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00018. panC. 1 hit.
ProtoNet	Search...
Other
NextBio	977232.

Entry information

Entry name

PANC_YEAST

Accession

Primary (citable) accession number: P40459
Secondary accession number(s): D6VVE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	December 20, 2005
Last modified:	April 3, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program