true1995-02-012023-11-08150ATG32_YEASTThe nucleotide sequence of Saccharomyces cerevisiae chromosome IX.Churcher C.M.Bowman S.Badcock K.Bankier A.T.Brown D.Chillingworth T.Connor R.Devlin K.Gentles S.Hamlin N.Harris D.E.Horsnell T.Hunt S.Jagels K.Jones M.Lye G.Moule S.Odell C.Pearson D.Rajandream M.A.Rice P.Rowley N.Skelton J.Smith V.Walsh S.V.Whitehead S.Barrell B.G.1997Nature38784-87NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]ATCC 204508 / S288cThe reference genome sequence of Saccharomyces cerevisiae: Then and now.Engel S.R.Dietrich F.S.Fisk D.G.Binkley G.Balakrishnan R.Costanzo M.C.Dwight S.S.Hitz B.C.Karra K.Nash R.S.Weng S.Wong E.D.Lloyd P.Skrzypek M.S.Miyasato S.R.Simison M.Cherry J.M.doi:10.1534/g3.113.0089952014G3 (Bethesda)4389-398GENOME REANNOTATIONApproaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.Hu Y.Rolfs A.Bhullar B.Murthy T.V.S.Zhu C.Berger M.F.Camargo A.A.Kelley F.McCarron S.Jepson D.Richardson A.Raphael J.Moreira D.Taycher E.Zuo D.Mohr S.Kane M.F.Williamson J.Simpson A.J.G.Bulyk M.L.Harlow E.Marsischky G.Kolodner R.D.LaBaer J.doi:10.1101/gr.60376072007Genome Res.17536-543NUCLEOTIDE SEQUENCE [GENOMIC DNA]Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae.Lussier M.White A.-M.Sheraton J.di Paolo T.Treadwell J.Southard S.B.Horenstein C.I.Chen-Weiner J.Ram A.F.J.Kapteyn J.C.Roemer T.W.Vo D.H.Bondoc D.C.Hall J.Zhong W.-W.Sdicu A.-M.Davies J.Klis F.M.Robbins P.W.Bussey H.doi:10.1093/genetics/147.2.4351997Genetics147435-450IDENTIFICATIONA landmark protein essential for mitophagy: Atg32 recruits the autophagic machinery to mitochondria.Okamoto K.Kondo-Okamoto N.Ohsumi Y.doi:10.4161/auto.5.8.98302009Autophagy51203-1205FUNCTIONMitochondria-anchored receptor Atg32 mediates degradation of mitochondria via selective autophagy.Okamoto K.Kondo-Okamoto N.Ohsumi Y.doi:10.1016/j.devcel.2009.06.0132009Dev. Cell1787-97SUBCELLULAR LOCATIONFUNCTIONINTERACTION WITH ATG8 AND ATG11Atg32 is a mitochondrial protein that confers selectivity during mitophagy.Kanki T.Wang K.Cao Y.Baba M.Klionsky D.J.doi:10.1016/j.devcel.2009.06.0142009Dev. Cell1798-109SUBCELLULAR LOCATIONFUNCTIONINTERACTION WITH ATG11A genomic screen for yeast mutants defective in selective mitochondria autophagy.Kanki T.Wang K.Baba M.Bartholomew C.R.Lynch-Day M.A.Du Z.Geng J.Mao K.Yang Z.Yen W.L.Klionsky D.J.doi:10.1091/mbc.e09-03-02252009Mol. Biol. Cell204730-4738FUNCTIONTwo MAPK-signaling pathways are required for mitophagy in Saccharomyces cerevisiae.Mao K.Wang K.Zhao M.Xu T.Klionsky D.J.doi:10.1083/jcb.2011020922011J. Cell Biol.193755-767FUNCTIONSUBCELLULAR LOCATIONPhosphorylation of Serine 114 on Atg32 mediates mitophagy.Aoki Y.Kanki T.Hirota Y.Kurihara Y.Saigusa T.Uchiumi T.Kang D.doi:10.1091/mbc.e11-02-01452011Mol. Biol. Cell223206-3217INTERACTION WITH ATG8 AND ATG11SUBCELLULAR LOCATIONPHOSPHORYLATION AT SER-114 AND SER-119MUTAGENESIS OF SER-114 AND SER-119SNCA (alpha-synuclein)-induced toxicity in yeast cells is dependent on sirtuin 2 (Sir2)-mediated mitophagy.Sampaio-Marques B.Felgueiras C.Silva A.Rodrigues M.Tenreiro S.Franssens V.Reichert A.S.Outeiro T.F.Winderickx J.Ludovico P.doi:10.4161/auto.212752012Autophagy81494-1509FUNCTIONPex3-anchored Atg36 tags peroxisomes for degradation in Saccharomyces cerevisiae.Motley A.M.Nuttall J.M.Hettema E.H.doi:10.1038/emboj.2012.1512012EMBO J.312852-2868FUNCTIONMitophagy plays an essential role in reducing mitochondrial production of reactive oxygen species and mutation of mitochondrial DNA by maintaining mitochondrial quantity and quality in yeast.Kurihara Y.Kanki T.Aoki Y.Hirota Y.Saigusa T.Uchiumi T.Kang D.doi:10.1074/jbc.m111.2801562012J. Biol. Chem.2873265-3272FUNCTIONAutophagy-related protein 32 acts as autophagic degron and directly initiates mitophagy.Kondo-Okamoto N.Noda N.N.Suzuki S.W.Nakatogawa H.Takahashi I.Matsunami M.Hashimoto A.Inagaki F.Ohsumi Y.Okamoto K.doi:10.1074/jbc.m111.2999172012J. Biol. Chem.28710631-10638X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 85-90FUNCTIONINTERACTION WITH ATG8 AND ATG113.00B=85-90A=200-3412486ATG32Eukaryota0 hits in 10 CRISPR screensProteinpsREC_Atg32Autophagy-related protein 32Extracellular mutant protein 37ATG32ECM17YIL146CMitophagy-specific receptor that recruits the autophagic machinery to mitochondria and regulates selective degradation of mitochondria. Mitophagy contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Recruits ATG11 to the surface of mitochondria. Promotes also autophagy-dependent peroxisome degradation.interacts with ATG8 and ATG11.Is recruited to the preautophagosomal structure during mitophagy and imported into the vacuole along with mitochondria during starvation.Phosphorylation of Ser-114 and Ser-119 are critically important for mitophagy and for the ATG11-ATG32 interaction. Phosphorylation depends on both HOG1 and PBS2.Belongs to the ATG32 family.Autophagy-related protein 32589691529Helical390414Disordered41Disordered345381Polar residues833Basic and acidic residues348366Basic residues367Phosphoserine114Phosphoserine119Abolishes mitophagy and impairs interaction with ATG11.ANGYDecreases mitophagy and impairs interaction with ATG11.A208210219225226229230232235238247251255261265277280283285288293299301304305307308310321323324327329338false2false2ATG11CKA11995-02-01158969b471806b6501110e73c68cbfe7e49a97MVLEYQQREGKGSSSKSMPPDSSSTTIHTCSEAQTGEDKGLLDPHLSVLELLSKTGHSPSPMGQNLVTSIDISGNHNVNDSISGSWQAIQPLDLGASFIPERCSSQTTNGSILSSSDTSEEEQELLQAPAADIINIIKQGQEGANVVSPSHPFKQLQKIISLPLPGKEKTPFNEQDDDGDEDEAFEEDSVTITKSLTSSTNSFVMPKLSLTQKNPVFRLLILGRTGSSFYQSIPKEYQSLFELPKYHDSATFPQYTGIVIIFQELREMVSLLNRIVQYSQGKPVIPICQPGQVIQVKNVLKSFLRNKLVKLLFPPVVVTNKRDLKKMFQRLQDLSLEYGEDVNEEDNDDEAIHTKSRSYCRNKKAENSKKKSPKSNKKPKRKKQKFFTSWFTWGISITIGISFGCCVTYFVTAAYEHQTVKSLSLRPSILASLLSLDSSSDTINTPATASPSSTEQFLWFDKGTLQINFHSDGFIMKSLTIIKETWGKMNTFVLHALSKPLKFLENLNKSSEFSIDESNRILALGYILLtruetruetruetruetruetruetruetruetruetruetruetruetruetruetrue