Autophagy-related protein 32 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P40458 (ATG32_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 88. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Autophagy-related protein 32

Alternative name(s):
Extracellular mutant protein 37

Gene names

Name:	ATG32
Synonyms:	ECM17
Ordered Locus Names:	YIL146C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	529 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Mitophagy-specific receptor that recruits the autophagic machinery to mitochondria and regulates selective degradation of mitochondria. Mitophagy contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Recruits ATG11 to the surface of mitochondria. Promotes also autophagy-dependent peroxisome degradation. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.11 Ref.12 Ref.13 Ref.14
Subunit structure	interacts with ATG8 and ATG11. Ref.6 Ref.7 Ref.10 Ref.14
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein. Vacuole membrane; Single-pass membrane protein. Preautophagosomal structure membrane; Single-pass membrane protein. Note: Is recruited to the preautophagosomal structure during mitophagy and imported into the vacuole along with mitochondria during starvation. Ref.6 Ref.7 Ref.9 Ref.10
Post-translational modification	Phosphorylation of Ser-114 and Ser-119 are critically important for mitophagy and for the ATG11-ATG32 interaction. Phosphorylation depends on both HOG1 and PBS2.
Sequence similarities	Belongs to the ATG32 family.

Ontologies

Keywords
Biological process	Autophagy
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane Vacuole
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrion degradation Inferred from mutant phenotype Ref.6 Ref.7. Source: SGD
Cellular_component	integral to mitochondrial outer membrane Inferred from direct assay Ref.6 Ref.7. Source: SGD vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 529

529

Autophagy-related protein 32

PRO_0000086920

Regions

Transmembrane

390 – 414

Helical; Potential

Amino acid modifications

Modified residue

114

Phosphoserine Ref.10

Modified residue

119

Phosphoserine Ref.10

Experimental info

Mutagenesis

114

S → A, N, G or Y: Abolishes mitophagy and impairs interaction with ATG11. Ref.10

Mutagenesis

119

S → A: Decreases mitophagy and impairs interaction with ATG11. Ref.10

Sequences

Sequence

Length

Mass (Da)

Tools

P40458 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: DB6A3DAFD50FB619
Show »

FASTA

529

58,969

        10         20         30         40         50         60 
MVLEYQQREG KGSSSKSMPP DSSSTTIHTC SEAQTGEDKG LLDPHLSVLE LLSKTGHSPS 

        70         80         90        100        110        120 
PMGQNLVTSI DISGNHNVND SISGSWQAIQ PLDLGASFIP ERCSSQTTNG SILSSSDTSE 

       130        140        150        160        170        180 
EEQELLQAPA ADIINIIKQG QEGANVVSPS HPFKQLQKII SLPLPGKEKT PFNEQDDDGD 

       190        200        210        220        230        240 
EDEAFEEDSV TITKSLTSST NSFVMPKLSL TQKNPVFRLL ILGRTGSSFY QSIPKEYQSL 

       250        260        270        280        290        300 
FELPKYHDSA TFPQYTGIVI IFQELREMVS LLNRIVQYSQ GKPVIPICQP GQVIQVKNVL 

       310        320        330        340        350        360 
KSFLRNKLVK LLFPPVVVTN KRDLKKMFQR LQDLSLEYGE DVNEEDNDDE AIHTKSRSYC 

       370        380        390        400        410        420 
RNKKAENSKK KSPKSNKKPK RKKQKFFTSW FTWGISITIG ISFGCCVTYF VTAAYEHQTV 

       430        440        450        460        470        480 
KSLSLRPSIL ASLLSLDSSS DTINTPATAS PSSTEQFLWF DKGTLQINFH SDGFIMKSLT 

       490        500        510        520 
IIKETWGKMN TFVLHALSKP LKFLENLNKS SEFSIDESNR ILALGYILL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae." Lussier M., White A.-M., Sheraton J., di Paolo T., Treadwell J., Southard S.B., Horenstein C.I., Chen-Weiner J., Ram A.F.J., Kapteyn J.C., Roemer T.W., Vo D.H., Bondoc D.C., Hall J., Zhong W.-W., Sdicu A.-M., Davies J., Klis F.M., Robbins P.W., Bussey H. Genetics 147:435-450(1997) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[5]	"A landmark protein essential for mitophagy: Atg32 recruits the autophagic machinery to mitochondria." Okamoto K., Kondo-Okamoto N., Ohsumi Y. Autophagy 5:1203-1205(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Mitochondria-anchored receptor Atg32 mediates degradation of mitochondria via selective autophagy." Okamoto K., Kondo-Okamoto N., Ohsumi Y. Dev. Cell 17:87-97(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH ATG8 AND ATG11.
[7]	"Atg32 is a mitochondrial protein that confers selectivity during mitophagy." Kanki T., Wang K., Cao Y., Baba M., Klionsky D.J. Dev. Cell 17:98-109(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH ATG11.
[8]	"A genomic screen for yeast mutants defective in selective mitochondria autophagy." Kanki T., Wang K., Baba M., Bartholomew C.R., Lynch-Day M.A., Du Z., Geng J., Mao K., Yang Z., Yen W.L., Klionsky D.J. Mol. Biol. Cell 20:4730-4738(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"Two MAPK-signaling pathways are required for mitophagy in Saccharomyces cerevisiae." Mao K., Wang K., Zhao M., Xu T., Klionsky D.J. J. Cell Biol. 193:755-767(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[10]	"Phosphorylation of Serine 114 on Atg32 mediates mitophagy." Aoki Y., Kanki T., Hirota Y., Kurihara Y., Saigusa T., Uchiumi T., Kang D. Mol. Biol. Cell 22:3206-3217(2011) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ATG8 AND ATG11, SUBCELLULAR LOCATION, PHOSPHORYLATION AT SER-114 AND SER-119, MUTAGENESIS OF SER-114 AND SER-119.
[11]	"SNCA (alpha-synuclein)-induced toxicity in yeast cells is dependent on sirtuin 2 (Sir2)-mediated mitophagy." Sampaio-Marques B., Felgueiras C., Silva A., Rodrigues M., Tenreiro S., Franssens V., Reichert A.S., Outeiro T.F., Winderickx J., Ludovico P. Autophagy 8:1494-1509(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[12]	"Pex3-anchored Atg36 tags peroxisomes for degradation in Saccharomyces cerevisiae." Motley A.M., Nuttall J.M., Hettema E.H. EMBO J. 31:2852-2868(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[13]	"Mitophagy plays an essential role in reducing mitochondrial production of reactive oxygen species and mutation of mitochondrial DNA by maintaining mitochondrial quantity and quality in yeast." Kurihara Y., Kanki T., Aoki Y., Hirota Y., Saigusa T., Uchiumi T., Kang D. J. Biol. Chem. 287:3265-3272(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[14]	"Autophagy-related protein 32 acts as autophagic degron and directly initiates mitophagy." Kondo-Okamoto N., Noda N.N., Suzuki S.W., Nakatogawa H., Takahashi I., Matsunami M., Hashimoto A., Inagaki F., Ohsumi Y., Okamoto K. J. Biol. Chem. 287:10631-10638(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 85-90, FUNCTION, INTERACTION WITH ATG8 AND ATG11.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z38059 Genomic DNA. Translation: CAA86132.1.
AY692933 Genomic DNA. Translation: AAT92952.1.
BK006942 Genomic DNA. Translation: DAA08407.1.

PIR

S48388.

RefSeq

NP_012120.1. NM_001179494.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3VXW	X-ray	3.00	B	85-90	[»]

ProteinModelPortal

P40458.

ModBase

Search...

Protein-protein interaction databases

IntAct

P40458. 2 interactions.

MINT

MINT-2781265.

STRING

4932.YIL146C.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YIL146C; YIL146C; YIL146C.

GeneID

854660.

KEGG

sce:YIL146C.

Organism-specific databases

CYGD

YIL146c.

SGD

S000001408. ATG32.

Phylogenomic databases

eggNOG

NOG44523.

OMA

NDINTHT.

OrthoDB

EOG476P8S.

Gene expression databases

Genevestigator

P40458.

GermOnline

YIL146C. Saccharomyces cerevisiae.

Family and domain databases

ProtoNet

Search...

Other

NextBio

977229.

Entry information

Entry name

ATG32_YEAST

Accession

Primary (citable) accession number: P40458
Secondary accession number(s): D6VVE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents