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P40458

- ATG32_YEAST

UniProt

P40458 - ATG32_YEAST

Protein

Autophagy-related protein 32

Gene

ATG32

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Mitophagy-specific receptor that recruits the autophagic machinery to mitochondria and regulates selective degradation of mitochondria. Mitophagy contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Recruits ATG11 to the surface of mitochondria. Promotes also autophagy-dependent peroxisome degradation.9 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. mitochondrion degradation Source: SGD

    Keywords - Biological processi

    Autophagy

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31395-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Autophagy-related protein 32
    Alternative name(s):
    Extracellular mutant protein 37
    Gene namesi
    Name:ATG32
    Synonyms:ECM17
    Ordered Locus Names:YIL146C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    CYGDiYIL146c.
    SGDiS000001408. ATG32.

    Subcellular locationi

    Mitochondrion outer membrane; Single-pass membrane protein. Vacuole membrane; Single-pass membrane protein. Preautophagosomal structure membrane; Single-pass membrane protein
    Note: Is recruited to the preautophagosomal structure during mitophagy and imported into the vacuole along with mitochondria during starvation.

    GO - Cellular componenti

    1. integral component of mitochondrial outer membrane Source: SGD
    2. pre-autophagosomal structure membrane Source: UniProtKB-SubCell
    3. vacuolar membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion outer membrane, Vacuole

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi114 – 1141S → A, N, G or Y: Abolishes mitophagy and impairs interaction with ATG11. 1 Publication
    Mutagenesisi119 – 1191S → A: Decreases mitophagy and impairs interaction with ATG11. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 529529Autophagy-related protein 32PRO_0000086920Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei114 – 1141Phosphoserine1 Publication
    Modified residuei119 – 1191Phosphoserine1 Publication

    Post-translational modificationi

    Phosphorylation of Ser-114 and Ser-119 are critically important for mitophagy and for the ATG11-ATG32 interaction. Phosphorylation depends on both HOG1 and PBS2.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP40458.

    Expressioni

    Gene expression databases

    GenevestigatoriP40458.

    Interactioni

    Subunit structurei

    interacts with ATG8 and ATG11.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ATG11Q125272EBI-25256,EBI-31977
    CKA1P157902EBI-25256,EBI-9533

    Protein-protein interaction databases

    BioGridi34846. 52 interactions.
    IntActiP40458. 6 interactions.
    MINTiMINT-2781265.
    STRINGi4932.YIL146C.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3VXWX-ray3.00B85-90[»]
    ProteinModelPortaliP40458.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei390 – 41425HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ATG32 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG44523.
    KOiK17984.
    OrthoDBiEOG7SN8QQ.

    Sequencei

    Sequence statusi: Complete.

    P40458-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVLEYQQREG KGSSSKSMPP DSSSTTIHTC SEAQTGEDKG LLDPHLSVLE    50
    LLSKTGHSPS PMGQNLVTSI DISGNHNVND SISGSWQAIQ PLDLGASFIP 100
    ERCSSQTTNG SILSSSDTSE EEQELLQAPA ADIINIIKQG QEGANVVSPS 150
    HPFKQLQKII SLPLPGKEKT PFNEQDDDGD EDEAFEEDSV TITKSLTSST 200
    NSFVMPKLSL TQKNPVFRLL ILGRTGSSFY QSIPKEYQSL FELPKYHDSA 250
    TFPQYTGIVI IFQELREMVS LLNRIVQYSQ GKPVIPICQP GQVIQVKNVL 300
    KSFLRNKLVK LLFPPVVVTN KRDLKKMFQR LQDLSLEYGE DVNEEDNDDE 350
    AIHTKSRSYC RNKKAENSKK KSPKSNKKPK RKKQKFFTSW FTWGISITIG 400
    ISFGCCVTYF VTAAYEHQTV KSLSLRPSIL ASLLSLDSSS DTINTPATAS 450
    PSSTEQFLWF DKGTLQINFH SDGFIMKSLT IIKETWGKMN TFVLHALSKP 500
    LKFLENLNKS SEFSIDESNR ILALGYILL 529
    Length:529
    Mass (Da):58,969
    Last modified:February 1, 1995 - v1
    Checksum:iDB6A3DAFD50FB619
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z38059 Genomic DNA. Translation: CAA86132.1.
    AY692933 Genomic DNA. Translation: AAT92952.1.
    BK006942 Genomic DNA. Translation: DAA08407.1.
    PIRiS48388.
    RefSeqiNP_012120.1. NM_001179494.1.

    Genome annotation databases

    EnsemblFungiiYIL146C; YIL146C; YIL146C.
    GeneIDi854660.
    KEGGisce:YIL146C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z38059 Genomic DNA. Translation: CAA86132.1 .
    AY692933 Genomic DNA. Translation: AAT92952.1 .
    BK006942 Genomic DNA. Translation: DAA08407.1 .
    PIRi S48388.
    RefSeqi NP_012120.1. NM_001179494.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3VXW X-ray 3.00 B 85-90 [» ]
    ProteinModelPortali P40458.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34846. 52 interactions.
    IntActi P40458. 6 interactions.
    MINTi MINT-2781265.
    STRINGi 4932.YIL146C.

    Proteomic databases

    MaxQBi P40458.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIL146C ; YIL146C ; YIL146C .
    GeneIDi 854660.
    KEGGi sce:YIL146C.

    Organism-specific databases

    CYGDi YIL146c.
    SGDi S000001408. ATG32.

    Phylogenomic databases

    eggNOGi NOG44523.
    KOi K17984.
    OrthoDBi EOG7SN8QQ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31395-MONOMER.

    Miscellaneous databases

    NextBioi 977229.

    Gene expression databases

    Genevestigatori P40458.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. "Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae."
      Lussier M., White A.-M., Sheraton J., di Paolo T., Treadwell J., Southard S.B., Horenstein C.I., Chen-Weiner J., Ram A.F.J., Kapteyn J.C., Roemer T.W., Vo D.H., Bondoc D.C., Hall J., Zhong W.-W., Sdicu A.-M., Davies J., Klis F.M., Robbins P.W., Bussey H.
      Genetics 147:435-450(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    5. "A landmark protein essential for mitophagy: Atg32 recruits the autophagic machinery to mitochondria."
      Okamoto K., Kondo-Okamoto N., Ohsumi Y.
      Autophagy 5:1203-1205(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Mitochondria-anchored receptor Atg32 mediates degradation of mitochondria via selective autophagy."
      Okamoto K., Kondo-Okamoto N., Ohsumi Y.
      Dev. Cell 17:87-97(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH ATG8 AND ATG11.
    7. "Atg32 is a mitochondrial protein that confers selectivity during mitophagy."
      Kanki T., Wang K., Cao Y., Baba M., Klionsky D.J.
      Dev. Cell 17:98-109(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH ATG11.
    8. "A genomic screen for yeast mutants defective in selective mitochondria autophagy."
      Kanki T., Wang K., Baba M., Bartholomew C.R., Lynch-Day M.A., Du Z., Geng J., Mao K., Yang Z., Yen W.L., Klionsky D.J.
      Mol. Biol. Cell 20:4730-4738(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "Two MAPK-signaling pathways are required for mitophagy in Saccharomyces cerevisiae."
      Mao K., Wang K., Zhao M., Xu T., Klionsky D.J.
      J. Cell Biol. 193:755-767(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    10. "Phosphorylation of Serine 114 on Atg32 mediates mitophagy."
      Aoki Y., Kanki T., Hirota Y., Kurihara Y., Saigusa T., Uchiumi T., Kang D.
      Mol. Biol. Cell 22:3206-3217(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ATG8 AND ATG11, SUBCELLULAR LOCATION, PHOSPHORYLATION AT SER-114 AND SER-119, MUTAGENESIS OF SER-114 AND SER-119.
    11. "SNCA (alpha-synuclein)-induced toxicity in yeast cells is dependent on sirtuin 2 (Sir2)-mediated mitophagy."
      Sampaio-Marques B., Felgueiras C., Silva A., Rodrigues M., Tenreiro S., Franssens V., Reichert A.S., Outeiro T.F., Winderickx J., Ludovico P.
      Autophagy 8:1494-1509(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "Pex3-anchored Atg36 tags peroxisomes for degradation in Saccharomyces cerevisiae."
      Motley A.M., Nuttall J.M., Hettema E.H.
      EMBO J. 31:2852-2868(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    13. "Mitophagy plays an essential role in reducing mitochondrial production of reactive oxygen species and mutation of mitochondrial DNA by maintaining mitochondrial quantity and quality in yeast."
      Kurihara Y., Kanki T., Aoki Y., Hirota Y., Saigusa T., Uchiumi T., Kang D.
      J. Biol. Chem. 287:3265-3272(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    14. "Autophagy-related protein 32 acts as autophagic degron and directly initiates mitophagy."
      Kondo-Okamoto N., Noda N.N., Suzuki S.W., Nakatogawa H., Takahashi I., Matsunami M., Hashimoto A., Inagaki F., Ohsumi Y., Okamoto K.
      J. Biol. Chem. 287:10631-10638(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 85-90, FUNCTION, INTERACTION WITH ATG8 AND ATG11.

    Entry informationi

    Entry nameiATG32_YEAST
    AccessioniPrimary (citable) accession number: P40458
    Secondary accession number(s): D6VVE1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3