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P40458 (ATG32_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Autophagy-related protein 32
Alternative name(s):
Extracellular mutant protein 37
Gene names
Name:ATG32
Synonyms:ECM17
Ordered Locus Names:YIL146C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length529 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Mitophagy-specific receptor that recruits the autophagic machinery to mitochondria and regulates selective degradation of mitochondria. Mitophagy contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Recruits ATG11 to the surface of mitochondria. Promotes also autophagy-dependent peroxisome degradation. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.11 Ref.12 Ref.13 Ref.14

Subunit structure

interacts with ATG8 and ATG11. Ref.6 Ref.7 Ref.10 Ref.14

Subcellular location

Mitochondrion outer membrane; Single-pass membrane protein. Vacuole membrane; Single-pass membrane protein. Preautophagosomal structure membrane; Single-pass membrane protein. Note: Is recruited to the preautophagosomal structure during mitophagy and imported into the vacuole along with mitochondria during starvation. Ref.6 Ref.7 Ref.9 Ref.10

Post-translational modification

Phosphorylation of Ser-114 and Ser-119 are critically important for mitophagy and for the ATG11-ATG32 interaction. Phosphorylation depends on both HOG1 and PBS2.

Sequence similarities

Belongs to the ATG32 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ATG11Q125272EBI-25256,EBI-31977
CKA1P157902EBI-25256,EBI-9533

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 529529Autophagy-related protein 32
PRO_0000086920

Regions

Transmembrane390 – 41425Helical; Potential

Amino acid modifications

Modified residue1141Phosphoserine Ref.10
Modified residue1191Phosphoserine Ref.10

Experimental info

Mutagenesis1141S → A, N, G or Y: Abolishes mitophagy and impairs interaction with ATG11. Ref.10
Mutagenesis1191S → A: Decreases mitophagy and impairs interaction with ATG11. Ref.10

Sequences

Sequence LengthMass (Da)Tools
P40458 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: DB6A3DAFD50FB619

FASTA52958,969
        10         20         30         40         50         60 
MVLEYQQREG KGSSSKSMPP DSSSTTIHTC SEAQTGEDKG LLDPHLSVLE LLSKTGHSPS 

        70         80         90        100        110        120 
PMGQNLVTSI DISGNHNVND SISGSWQAIQ PLDLGASFIP ERCSSQTTNG SILSSSDTSE 

       130        140        150        160        170        180 
EEQELLQAPA ADIINIIKQG QEGANVVSPS HPFKQLQKII SLPLPGKEKT PFNEQDDDGD 

       190        200        210        220        230        240 
EDEAFEEDSV TITKSLTSST NSFVMPKLSL TQKNPVFRLL ILGRTGSSFY QSIPKEYQSL 

       250        260        270        280        290        300 
FELPKYHDSA TFPQYTGIVI IFQELREMVS LLNRIVQYSQ GKPVIPICQP GQVIQVKNVL 

       310        320        330        340        350        360 
KSFLRNKLVK LLFPPVVVTN KRDLKKMFQR LQDLSLEYGE DVNEEDNDDE AIHTKSRSYC 

       370        380        390        400        410        420 
RNKKAENSKK KSPKSNKKPK RKKQKFFTSW FTWGISITIG ISFGCCVTYF VTAAYEHQTV 

       430        440        450        460        470        480 
KSLSLRPSIL ASLLSLDSSS DTINTPATAS PSSTEQFLWF DKGTLQINFH SDGFIMKSLT 

       490        500        510        520 
IIKETWGKMN TFVLHALSKP LKFLENLNKS SEFSIDESNR ILALGYILL 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae."
Lussier M., White A.-M., Sheraton J., di Paolo T., Treadwell J., Southard S.B., Horenstein C.I., Chen-Weiner J., Ram A.F.J., Kapteyn J.C., Roemer T.W., Vo D.H., Bondoc D.C., Hall J., Zhong W.-W., Sdicu A.-M., Davies J., Klis F.M., Robbins P.W., Bussey H.
Genetics 147:435-450(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
[5]"A landmark protein essential for mitophagy: Atg32 recruits the autophagic machinery to mitochondria."
Okamoto K., Kondo-Okamoto N., Ohsumi Y.
Autophagy 5:1203-1205(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Mitochondria-anchored receptor Atg32 mediates degradation of mitochondria via selective autophagy."
Okamoto K., Kondo-Okamoto N., Ohsumi Y.
Dev. Cell 17:87-97(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH ATG8 AND ATG11.
[7]"Atg32 is a mitochondrial protein that confers selectivity during mitophagy."
Kanki T., Wang K., Cao Y., Baba M., Klionsky D.J.
Dev. Cell 17:98-109(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, FUNCTION, INTERACTION WITH ATG11.
[8]"A genomic screen for yeast mutants defective in selective mitochondria autophagy."
Kanki T., Wang K., Baba M., Bartholomew C.R., Lynch-Day M.A., Du Z., Geng J., Mao K., Yang Z., Yen W.L., Klionsky D.J.
Mol. Biol. Cell 20:4730-4738(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Two MAPK-signaling pathways are required for mitophagy in Saccharomyces cerevisiae."
Mao K., Wang K., Zhao M., Xu T., Klionsky D.J.
J. Cell Biol. 193:755-767(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[10]"Phosphorylation of Serine 114 on Atg32 mediates mitophagy."
Aoki Y., Kanki T., Hirota Y., Kurihara Y., Saigusa T., Uchiumi T., Kang D.
Mol. Biol. Cell 22:3206-3217(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ATG8 AND ATG11, SUBCELLULAR LOCATION, PHOSPHORYLATION AT SER-114 AND SER-119, MUTAGENESIS OF SER-114 AND SER-119.
[11]"SNCA (alpha-synuclein)-induced toxicity in yeast cells is dependent on sirtuin 2 (Sir2)-mediated mitophagy."
Sampaio-Marques B., Felgueiras C., Silva A., Rodrigues M., Tenreiro S., Franssens V., Reichert A.S., Outeiro T.F., Winderickx J., Ludovico P.
Autophagy 8:1494-1509(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"Pex3-anchored Atg36 tags peroxisomes for degradation in Saccharomyces cerevisiae."
Motley A.M., Nuttall J.M., Hettema E.H.
EMBO J. 31:2852-2868(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[13]"Mitophagy plays an essential role in reducing mitochondrial production of reactive oxygen species and mutation of mitochondrial DNA by maintaining mitochondrial quantity and quality in yeast."
Kurihara Y., Kanki T., Aoki Y., Hirota Y., Saigusa T., Uchiumi T., Kang D.
J. Biol. Chem. 287:3265-3272(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[14]"Autophagy-related protein 32 acts as autophagic degron and directly initiates mitophagy."
Kondo-Okamoto N., Noda N.N., Suzuki S.W., Nakatogawa H., Takahashi I., Matsunami M., Hashimoto A., Inagaki F., Ohsumi Y., Okamoto K.
J. Biol. Chem. 287:10631-10638(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 85-90, FUNCTION, INTERACTION WITH ATG8 AND ATG11.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z38059 Genomic DNA. Translation: CAA86132.1.
AY692933 Genomic DNA. Translation: AAT92952.1.
BK006942 Genomic DNA. Translation: DAA08407.1.
PIRS48388.
RefSeqNP_012120.1. NM_001179494.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3VXWX-ray3.00B85-90[»]
ProteinModelPortalP40458.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34846. 52 interactions.
IntActP40458. 6 interactions.
MINTMINT-2781265.
STRING4932.YIL146C.

Proteomic databases

MaxQBP40458.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYIL146C; YIL146C; YIL146C.
GeneID854660.
KEGGsce:YIL146C.

Organism-specific databases

CYGDYIL146c.
SGDS000001408. ATG32.

Phylogenomic databases

eggNOGNOG44523.
KOK17984.
OrthoDBEOG7SN8QQ.

Enzyme and pathway databases

BioCycYEAST:G3O-31395-MONOMER.

Gene expression databases

GenevestigatorP40458.

Family and domain databases

ProtoNetSearch...

Other

NextBio977229.

Entry information

Entry nameATG32_YEAST
AccessionPrimary (citable) accession number: P40458
Secondary accession number(s): D6VVE1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references