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P40457

- MLP2_YEAST

UniProt

P40457 - MLP2_YEAST

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Protein

Protein MLP2

Gene

MLP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved together with the closely related MLP1 in the structural and functional organization of perinuclear chromatin. MLP1/MLP2 associate with the nuclear pore complex and form filamentous structures along the nuclear periphery. According to PubMed:11862215 some telomeres are tethered to the nuclear periphery through MLP1/MLP2, probably mediated by YKU70/YKU80 (HDF1/HDF2) heterodimer and show perinuclear location dependent silencing. According to PubMed:12490156 MLP1 and MLP2 are involved in telomere length regulation but not silencing or telomere anchoring. MLP2 plays a role in the incorporation of components into the spindle pole body. MLP2 is also involved in double-strand break repair, probably also mediated by the YKU70/YKU80 (HDF1/HDF2) heterodimer.7 Publications

GO - Molecular functioni

  1. ribonucleoprotein complex binding Source: SGD

GO - Biological processi

  1. negative regulation of protein import into nucleus during spindle assembly checkpoint Source: SGD
  2. poly(A)+ mRNA export from nucleus Source: SGD
  3. posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery Source: SGD
  4. protein import into nucleus Source: InterPro
  5. regulation of transcription, DNA-templated Source: SGD
  6. spindle pole body organization Source: SGD
  7. telomere tethering at nuclear periphery Source: SGD
  8. transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-31398-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein MLP2
Alternative name(s):
Myosin-like protein 2
Gene namesi
Name:MLP2
Ordered Locus Names:YIL149C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IX

Organism-specific databases

CYGDiYIL149c.
SGDiS000001411. MLP2.

Subcellular locationi

Nucleus. Cytoplasmcytoskeletonmicrotubule organizing centerspindle pole body
Note: Nuclear periphery, excluded from nuclear envelope adjacent to nucleolus.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nuclear envelope Source: SGD
  3. nuclear pore nuclear basket Source: SGD
  4. nucleoplasm Source: SGD
  5. spindle pole body Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 16791679Protein MLP2PRO_0000096502Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1512 – 15121Phosphoserine1 Publication
Modified residuei1670 – 16701Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40457.
PaxDbiP40457.
PRIDEiP40457.

Expressioni

Gene expression databases

GenevestigatoriP40457.

Interactioni

Subunit structurei

Component of the spindle pole body core in which it interacts directly with SPC110, SPC42 and SPC29. Interacts with NUP60 and NIC96, which tether it to the nuclear pore complex. Also interacts with YKU70 (HDF1) and MLP1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MLP1Q024552EBI-25261,EBI-11009
SPC110P323803EBI-25261,EBI-12369
SPC29P334192EBI-25261,EBI-12041
SPC42P360943EBI-25261,EBI-17777

Protein-protein interaction databases

BioGridi34843. 92 interactions.
DIPiDIP-2388N.
IntActiP40457. 13 interactions.
MINTiMINT-707166.
STRINGi4932.YIL149C.

Structurei

3D structure databases

ProteinModelPortaliP40457.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili32 – 176145Sequence AnalysisAdd
BLAST
Coiled coili233 – 466234Sequence AnalysisAdd
BLAST
Coiled coili516 – 1064549Sequence AnalysisAdd
BLAST
Coiled coili1099 – 1491393Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi417 – 43317Bipartite nuclear localization signal 1Add
BLAST
Motifi639 – 65517Bipartite nuclear localization signal 2Add
BLAST
Motifi1433 – 144917Bipartite nuclear localization signal 3Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi467 – 4704Poly-Leu
Compositional biasi1393 – 1508116Glu-richAdd
BLAST

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG12793.
GeneTreeiENSGT00730000111014.
InParanoidiP40457.
KOiK09291.
OMAiMETRINI.
OrthoDBiEOG7BP89V.

Family and domain databases

InterProiIPR012929. TPR_MLP1_2.
[Graphical view]
PfamiPF07926. TPR_MLP1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40457-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEDKISEFLN VPFESLQGVT YPVLRKLYKK IAKFERSEEE VTKLNVLVDE
60 70 80 90 100
IKSQYYSRIS KLKQLLDESS EQKNTAKEEL NGLKDQLNEE RSRYRREIDA
110 120 130 140 150
LKKQLHVSHE AMREVNDEKR VKEEYDIWQS RDQGNDSLND DLNKENKLLR
160 170 180 190 200
RKLMEMENIL QRCKSNAISL QLKYDTSVQE KELMLQSKKL IEEKLSSFSK
210 220 230 240 250
KTLTEEVTKS SHVENLEEKL YQMQSNYESV FTYNKFLLNQ NKQLSQSVEE
260 270 280 290 300
KVLEMKNLKD TASVEKAEFS KEMTLQKNMN DLLRSQLTSL EKDCSLRAIE
310 320 330 340 350
KNDDNSCRNP EHTDVIDELI DTKLRLEKSK NECQRLQNIV MDCTKEEEAT
360 370 380 390 400
MTTSAVSPTV GKLFSDIKVL KRQLIKERNQ KFQLQNQLED FILELEHKTP
410 420 430 440 450
ELISFKERTK SLEHELKRST ELLETVSLTK RKQEREITSL RQKINGCEAN
460 470 480 490 500
IHSLVKQRLD LARQVKLLLL NTSAIQETAS PLSQDELISL RKILESSNIV
510 520 530 540 550
NENDSQAIIT ERLVEFSNVN ELQEKNVELL NCIRILADKL ENYEGKQDKT
560 570 580 590 600
LQKVENQTIK EAKDAIIELE NINAKMETRI NILLRERDSY KLLASTEENK
610 620 630 640 650
ANTNSVTSME AAREKKIREL EAELSSTKVE NSAIIQNLRK ELLIYKKSQC
660 670 680 690 700
KKKTTLEDFE NFKGLAKEKE RMLEEAIDHL KAELEKQKSW VPSYIHVEKE
710 720 730 740 750
RASTELSQSR IKIKSLEYEI SKLKKETASF IPTKESLTRD FEQCCKEKKE
760 770 780 790 800
LQMRLKESEI SHNENKMDFS SKEGQYKAKI KELENNLERL RSDLQSKIQE
810 820 830 840 850
IESIRSCKDS QLKWAQNTID DTEMKMKSLL TELSNKETTI EKLSSEIENL
860 870 880 890 900
DKELRKTKFQ YKFLDQNSDA STLEPTLRKE LEQIQVQLKD ANSQIQAYEE
910 920 930 940 950
IISSNENALI ELKNELAKTK ENYDAKIELE KKEKWAREED LSRLRGELGE
960 970 980 990 1000
IRALQPKLKE GALHFVQQSE KLRNEVERIQ KMIEKIEKMS TIVQLCKKKE
1010 1020 1030 1040 1050
MSQYQSTMKE NKDLSELVIR LEKDAADCQA ELTKTKSSLY SAQDLLDKHE
1060 1070 1080 1090 1100
RKWMEEKADY ERELISNIEQ TESLRVENSV LIEKVDDTAA NNGDKDHLKL
1110 1120 1130 1140 1150
VSLFSNLRHE RNSLETKLTT CKRELAFVKQ KNDSLEKTIN DLQRTQTLSE
1160 1170 1180 1190 1200
KEYQCSAVII DEFKDITKEV TQVNILKENN AILQKSLKNV TEKNREIYKQ
1210 1220 1230 1240 1250
LNDRQEEISR LQRDLIQTKE QVSINSNKIL VYESEMEQCK QRYQDLSQQQ
1260 1270 1280 1290 1300
KDAQKKDIEK LTNEISDLKG KLSSAENANA DLENKFNRLK KQAHEKLDAS
1310 1320 1330 1340 1350
KKQQAALTNE LNELKAIKDK LEQDLHFENA KVIDLDTKLK AHELQSEDVS
1360 1370 1380 1390 1400
RDHEKDTYRT LMEEIESLKK ELQIFKTANS SSDAFEKLKV NMEKEKDRII
1410 1420 1430 1440 1450
DERTKEFEKK LQETLNKSTS SEAEYSKDIE TLKKEWLKEY EDETLRRIKE
1460 1470 1480 1490 1500
AEENLKKRIR LPSEERIQKI ISKRKEELEE EFRKKLKENA GSLTFLDNKG
1510 1520 1530 1540 1550
SGEDAEEELW NSPSKGNSER PSAVAGFINQ KNLKPQEQLK NVKNDVSFND
1560 1570 1580 1590 1600
SQSMVTNKEN NIVDSSAAGN KAIPTFSFGK PFFSSNTSSL QSFQNPFTAS
1610 1620 1630 1640 1650
QSNINTNAPL RTLNIQPEVA VKAAINFSNV TDLTNNSTDG AKITEIGSTS
1660 1670
KRPIESGTSS DPDTKKVKES PANDQASNE
Length:1,679
Mass (Da):195,141
Last modified:February 1, 1995 - v1
Checksum:i298950CC52202D8F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z38059 Genomic DNA. Translation: CAA86129.1.
BK006942 Genomic DNA. Translation: DAA08404.1.
PIRiS48385.
RefSeqiNP_012117.3. NM_001179497.3.

Genome annotation databases

EnsemblFungiiYIL149C; YIL149C; YIL149C.
GeneIDi854657.
KEGGisce:YIL149C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z38059 Genomic DNA. Translation: CAA86129.1 .
BK006942 Genomic DNA. Translation: DAA08404.1 .
PIRi S48385.
RefSeqi NP_012117.3. NM_001179497.3.

3D structure databases

ProteinModelPortali P40457.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34843. 92 interactions.
DIPi DIP-2388N.
IntActi P40457. 13 interactions.
MINTi MINT-707166.
STRINGi 4932.YIL149C.

Proteomic databases

MaxQBi P40457.
PaxDbi P40457.
PRIDEi P40457.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YIL149C ; YIL149C ; YIL149C .
GeneIDi 854657.
KEGGi sce:YIL149C.

Organism-specific databases

CYGDi YIL149c.
SGDi S000001411. MLP2.

Phylogenomic databases

eggNOGi NOG12793.
GeneTreei ENSGT00730000111014.
InParanoidi P40457.
KOi K09291.
OMAi METRINI.
OrthoDBi EOG7BP89V.

Enzyme and pathway databases

BioCyci YEAST:G3O-31398-MONOMER.

Miscellaneous databases

NextBioi 977220.
PROi P40457.

Gene expression databases

Genevestigatori P40457.

Family and domain databases

InterProi IPR012929. TPR_MLP1_2.
[Graphical view ]
Pfami PF07926. TPR_MLP1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Proteins connecting the nuclear pore complex with the nuclear interior."
    Strambio-de-Castillia C., Blobel G., Rout M.P.
    J. Cell Biol. 144:839-855(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, FORMATION OF CHROMATIN EXCLUDING FILAMENTOUS STRUCTURES WITH MLP1.
  4. "Mlp2p, a component of nuclear pore attached intranuclear filaments, associates with nic96p."
    Kosova B., Pante N., Rollenhagen C., Podtelejnikov A., Mann M., Aebi U., Hurt E.C.
    J. Biol. Chem. 275:343-350(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH NIC96.
  5. "Nuclear pore complexes in the organization of silent telomeric chromatin."
    Galy V., Olivo-Marin J.-C., Scherthan H., Doye V., Rascalou N., Nehrbass U.
    Nature 403:108-112(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PERINUCLEAR TELOMERE CLUSTERING, DOUBLE-STRAND BREAK REPAIR, INTERACTION WITH YKU70 (HDF1).
  6. "Nuclear architecture and spatial positioning help establish transcriptional states of telomeres in yeast."
    Feuerbach F., Galy V., Trelles-Sticken E., Fromont-Racine M., Jacquier A., Gilson E., Olivo-Marin J.-C., Scherthan H., Nehrbass U.
    Nat. Cell Biol. 4:214-221(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PERINUCLEAR-DEPENDENT SILENCING, INTERACTION WITH NUP60.
  7. "Myosin-like proteins 1 and 2 are not required for silencing or telomere anchoring, but act in the Tel1 pathway of telomere length control."
    Hediger F., Dubrana K., Gasser S.M.
    J. Struct. Biol. 140:79-91(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TELOMERE LENGTH REGULATION.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Nuclear retention of unspliced mRNAs in yeast is mediated by perinuclear Mlp1."
    Galy V., Gadal O., Fromont-Racine M., Romano A., Jacquier A., Nehrbass U.
    Cell 116:63-73(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  10. "The nuclear pore complex-associated protein, Mlp2p, binds to the yeast spindle pole body and promotes its efficient assembly."
    Niepel M., Strambio-de-Castillia C., Fasolo J., Chait B.T., Rout M.P.
    J. Cell Biol. 170:225-235(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE SPINDLE POLE BODY CORE COMPLEX, INTERACTION WITH MLP1; SPC110; SPC42 AND SPC29, SUBCELLULAR LOCATION, FUNCTION.
  11. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1512, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1670, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMLP2_YEAST
AccessioniPrimary (citable) accession number: P40457
Secondary accession number(s): D6VVD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2770 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3