Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P40457

- MLP2_YEAST

UniProt

P40457 - MLP2_YEAST

Protein

Protein MLP2

Gene

MLP2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved together with the closely related MLP1 in the structural and functional organization of perinuclear chromatin. MLP1/MLP2 associate with the nuclear pore complex and form filamentous structures along the nuclear periphery. According to PubMed:11862215 some telomeres are tethered to the nuclear periphery through MLP1/MLP2, probably mediated by YKU70/YKU80 (HDF1/HDF2) heterodimer and show perinuclear location dependent silencing. According to PubMed:12490156 MLP1 and MLP2 are involved in telomere length regulation but not silencing or telomere anchoring. MLP2 plays a role in the incorporation of components into the spindle pole body. MLP2 is also involved in double-strand break repair, probably also mediated by the YKU70/YKU80 (HDF1/HDF2) heterodimer.7 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. ribonucleoprotein complex binding Source: SGD

    GO - Biological processi

    1. negative regulation of protein import into nucleus during spindle assembly checkpoint Source: SGD
    2. poly(A)+ mRNA export from nucleus Source: SGD
    3. posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery Source: SGD
    4. protein import into nucleus Source: InterPro
    5. regulation of transcription, DNA-templated Source: SGD
    6. spindle pole body organization Source: SGD
    7. telomere tethering at nuclear periphery Source: SGD
    8. transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31398-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein MLP2
    Alternative name(s):
    Myosin-like protein 2
    Gene namesi
    Name:MLP2
    Ordered Locus Names:YIL149C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    CYGDiYIL149c.
    SGDiS000001411. MLP2.

    Subcellular locationi

    Nucleus. Cytoplasmcytoskeletonmicrotubule organizing centerspindle pole body
    Note: Nuclear periphery, excluded from nuclear envelope adjacent to nucleolus.

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-KW
    2. nuclear envelope Source: SGD
    3. nuclear pore nuclear basket Source: SGD
    4. nucleoplasm Source: SGD
    5. spindle pole body Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 16791679Protein MLP2PRO_0000096502Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1512 – 15121Phosphoserine1 Publication
    Modified residuei1670 – 16701Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP40457.
    PaxDbiP40457.
    PRIDEiP40457.

    Expressioni

    Gene expression databases

    GenevestigatoriP40457.

    Interactioni

    Subunit structurei

    Component of the spindle pole body core in which it interacts directly with SPC110, SPC42 and SPC29. Interacts with NUP60 and NIC96, which tether it to the nuclear pore complex. Also interacts with YKU70 (HDF1) and MLP1.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MLP1Q024552EBI-25261,EBI-11009
    SPC110P323803EBI-25261,EBI-12369
    SPC29P334192EBI-25261,EBI-12041
    SPC42P360943EBI-25261,EBI-17777

    Protein-protein interaction databases

    BioGridi34843. 91 interactions.
    DIPiDIP-2388N.
    IntActiP40457. 13 interactions.
    MINTiMINT-707166.
    STRINGi4932.YIL149C.

    Structurei

    3D structure databases

    ProteinModelPortaliP40457.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili32 – 176145Sequence AnalysisAdd
    BLAST
    Coiled coili233 – 466234Sequence AnalysisAdd
    BLAST
    Coiled coili516 – 1064549Sequence AnalysisAdd
    BLAST
    Coiled coili1099 – 1491393Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi417 – 43317Bipartite nuclear localization signal 1Add
    BLAST
    Motifi639 – 65517Bipartite nuclear localization signal 2Add
    BLAST
    Motifi1433 – 144917Bipartite nuclear localization signal 3Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi467 – 4704Poly-Leu
    Compositional biasi1393 – 1508116Glu-richAdd
    BLAST

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG12793.
    GeneTreeiENSGT00730000111014.
    KOiK09291.
    OMAiMETRINI.
    OrthoDBiEOG7BP89V.

    Family and domain databases

    InterProiIPR012929. TPR_MLP1_2.
    [Graphical view]
    PfamiPF07926. TPR_MLP1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40457-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEDKISEFLN VPFESLQGVT YPVLRKLYKK IAKFERSEEE VTKLNVLVDE     50
    IKSQYYSRIS KLKQLLDESS EQKNTAKEEL NGLKDQLNEE RSRYRREIDA 100
    LKKQLHVSHE AMREVNDEKR VKEEYDIWQS RDQGNDSLND DLNKENKLLR 150
    RKLMEMENIL QRCKSNAISL QLKYDTSVQE KELMLQSKKL IEEKLSSFSK 200
    KTLTEEVTKS SHVENLEEKL YQMQSNYESV FTYNKFLLNQ NKQLSQSVEE 250
    KVLEMKNLKD TASVEKAEFS KEMTLQKNMN DLLRSQLTSL EKDCSLRAIE 300
    KNDDNSCRNP EHTDVIDELI DTKLRLEKSK NECQRLQNIV MDCTKEEEAT 350
    MTTSAVSPTV GKLFSDIKVL KRQLIKERNQ KFQLQNQLED FILELEHKTP 400
    ELISFKERTK SLEHELKRST ELLETVSLTK RKQEREITSL RQKINGCEAN 450
    IHSLVKQRLD LARQVKLLLL NTSAIQETAS PLSQDELISL RKILESSNIV 500
    NENDSQAIIT ERLVEFSNVN ELQEKNVELL NCIRILADKL ENYEGKQDKT 550
    LQKVENQTIK EAKDAIIELE NINAKMETRI NILLRERDSY KLLASTEENK 600
    ANTNSVTSME AAREKKIREL EAELSSTKVE NSAIIQNLRK ELLIYKKSQC 650
    KKKTTLEDFE NFKGLAKEKE RMLEEAIDHL KAELEKQKSW VPSYIHVEKE 700
    RASTELSQSR IKIKSLEYEI SKLKKETASF IPTKESLTRD FEQCCKEKKE 750
    LQMRLKESEI SHNENKMDFS SKEGQYKAKI KELENNLERL RSDLQSKIQE 800
    IESIRSCKDS QLKWAQNTID DTEMKMKSLL TELSNKETTI EKLSSEIENL 850
    DKELRKTKFQ YKFLDQNSDA STLEPTLRKE LEQIQVQLKD ANSQIQAYEE 900
    IISSNENALI ELKNELAKTK ENYDAKIELE KKEKWAREED LSRLRGELGE 950
    IRALQPKLKE GALHFVQQSE KLRNEVERIQ KMIEKIEKMS TIVQLCKKKE 1000
    MSQYQSTMKE NKDLSELVIR LEKDAADCQA ELTKTKSSLY SAQDLLDKHE 1050
    RKWMEEKADY ERELISNIEQ TESLRVENSV LIEKVDDTAA NNGDKDHLKL 1100
    VSLFSNLRHE RNSLETKLTT CKRELAFVKQ KNDSLEKTIN DLQRTQTLSE 1150
    KEYQCSAVII DEFKDITKEV TQVNILKENN AILQKSLKNV TEKNREIYKQ 1200
    LNDRQEEISR LQRDLIQTKE QVSINSNKIL VYESEMEQCK QRYQDLSQQQ 1250
    KDAQKKDIEK LTNEISDLKG KLSSAENANA DLENKFNRLK KQAHEKLDAS 1300
    KKQQAALTNE LNELKAIKDK LEQDLHFENA KVIDLDTKLK AHELQSEDVS 1350
    RDHEKDTYRT LMEEIESLKK ELQIFKTANS SSDAFEKLKV NMEKEKDRII 1400
    DERTKEFEKK LQETLNKSTS SEAEYSKDIE TLKKEWLKEY EDETLRRIKE 1450
    AEENLKKRIR LPSEERIQKI ISKRKEELEE EFRKKLKENA GSLTFLDNKG 1500
    SGEDAEEELW NSPSKGNSER PSAVAGFINQ KNLKPQEQLK NVKNDVSFND 1550
    SQSMVTNKEN NIVDSSAAGN KAIPTFSFGK PFFSSNTSSL QSFQNPFTAS 1600
    QSNINTNAPL RTLNIQPEVA VKAAINFSNV TDLTNNSTDG AKITEIGSTS 1650
    KRPIESGTSS DPDTKKVKES PANDQASNE 1679
    Length:1,679
    Mass (Da):195,141
    Last modified:February 1, 1995 - v1
    Checksum:i298950CC52202D8F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z38059 Genomic DNA. Translation: CAA86129.1.
    BK006942 Genomic DNA. Translation: DAA08404.1.
    PIRiS48385.
    RefSeqiNP_012117.3. NM_001179497.3.

    Genome annotation databases

    EnsemblFungiiYIL149C; YIL149C; YIL149C.
    GeneIDi854657.
    KEGGisce:YIL149C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z38059 Genomic DNA. Translation: CAA86129.1 .
    BK006942 Genomic DNA. Translation: DAA08404.1 .
    PIRi S48385.
    RefSeqi NP_012117.3. NM_001179497.3.

    3D structure databases

    ProteinModelPortali P40457.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34843. 91 interactions.
    DIPi DIP-2388N.
    IntActi P40457. 13 interactions.
    MINTi MINT-707166.
    STRINGi 4932.YIL149C.

    Proteomic databases

    MaxQBi P40457.
    PaxDbi P40457.
    PRIDEi P40457.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIL149C ; YIL149C ; YIL149C .
    GeneIDi 854657.
    KEGGi sce:YIL149C.

    Organism-specific databases

    CYGDi YIL149c.
    SGDi S000001411. MLP2.

    Phylogenomic databases

    eggNOGi NOG12793.
    GeneTreei ENSGT00730000111014.
    KOi K09291.
    OMAi METRINI.
    OrthoDBi EOG7BP89V.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31398-MONOMER.

    Miscellaneous databases

    NextBioi 977220.
    PROi P40457.

    Gene expression databases

    Genevestigatori P40457.

    Family and domain databases

    InterProi IPR012929. TPR_MLP1_2.
    [Graphical view ]
    Pfami PF07926. TPR_MLP1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Proteins connecting the nuclear pore complex with the nuclear interior."
      Strambio-de-Castillia C., Blobel G., Rout M.P.
      J. Cell Biol. 144:839-855(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, FORMATION OF CHROMATIN EXCLUDING FILAMENTOUS STRUCTURES WITH MLP1.
    4. "Mlp2p, a component of nuclear pore attached intranuclear filaments, associates with nic96p."
      Kosova B., Pante N., Rollenhagen C., Podtelejnikov A., Mann M., Aebi U., Hurt E.C.
      J. Biol. Chem. 275:343-350(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH NIC96.
    5. "Nuclear pore complexes in the organization of silent telomeric chromatin."
      Galy V., Olivo-Marin J.-C., Scherthan H., Doye V., Rascalou N., Nehrbass U.
      Nature 403:108-112(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PERINUCLEAR TELOMERE CLUSTERING, DOUBLE-STRAND BREAK REPAIR, INTERACTION WITH YKU70 (HDF1).
    6. "Nuclear architecture and spatial positioning help establish transcriptional states of telomeres in yeast."
      Feuerbach F., Galy V., Trelles-Sticken E., Fromont-Racine M., Jacquier A., Gilson E., Olivo-Marin J.-C., Scherthan H., Nehrbass U.
      Nat. Cell Biol. 4:214-221(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PERINUCLEAR-DEPENDENT SILENCING, INTERACTION WITH NUP60.
    7. "Myosin-like proteins 1 and 2 are not required for silencing or telomere anchoring, but act in the Tel1 pathway of telomere length control."
      Hediger F., Dubrana K., Gasser S.M.
      J. Struct. Biol. 140:79-91(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TELOMERE LENGTH REGULATION.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Nuclear retention of unspliced mRNAs in yeast is mediated by perinuclear Mlp1."
      Galy V., Gadal O., Fromont-Racine M., Romano A., Jacquier A., Nehrbass U.
      Cell 116:63-73(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    10. "The nuclear pore complex-associated protein, Mlp2p, binds to the yeast spindle pole body and promotes its efficient assembly."
      Niepel M., Strambio-de-Castillia C., Fasolo J., Chait B.T., Rout M.P.
      J. Cell Biol. 170:225-235(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE SPINDLE POLE BODY CORE COMPLEX, INTERACTION WITH MLP1; SPC110; SPC42 AND SPC29, SUBCELLULAR LOCATION, FUNCTION.
    11. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1512, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1670, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMLP2_YEAST
    AccessioniPrimary (citable) accession number: P40457
    Secondary accession number(s): D6VVD8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2770 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3