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Protein

Ubiquitin carboxyl-terminal hydrolase 7

Gene

UBP7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the sorting of ubiquitinated cargo proteins at the multivesicular body (MVB).1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei618 – 6181NucleophilePROSITE-ProRule annotation
Active sitei1014 – 10141Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  • ubiquitin-specific protease activity Source: SGD

GO - Biological processi

  • protein deubiquitination Source: SGD
  • ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-31404-MONOMER.

Protein family/group databases

MEROPSiC19.101.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 7 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 7
Ubiquitin thioesterase 7
Ubiquitin-specific-processing protease 7
Gene namesi
Name:UBP7
Ordered Locus Names:YIL156W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome IX

Organism-specific databases

CYGDiYIL156w.
EuPathDBiFungiDB:YIL156W.
SGDiS000001418. UBP7.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10711071Ubiquitin carboxyl-terminal hydrolase 7PRO_0000080592Add
BLAST

Proteomic databases

MaxQBiP40453.
PaxDbiP40453.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
BZZ1P388223EBI-19857,EBI-3889
HOF1Q050802EBI-19857,EBI-5412
HSE1P387534EBI-19857,EBI-1382
LSB3P436032EBI-19857,EBI-22980
MYO3P360063EBI-19857,EBI-11670
MYO5Q044394EBI-19857,EBI-11687
PEX13P806672EBI-19857,EBI-13206

Protein-protein interaction databases

BioGridi34836. 53 interactions.
DIPiDIP-6272N.
IntActiP40453. 13 interactions.
MINTiMINT-598347.

Structurei

3D structure databases

ProteinModelPortaliP40453.
SMRiP40453. Positions 593-884, 943-1071.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini609 – 1069461USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiCOG5533.
GeneTreeiENSGT00670000099202.
HOGENOMiHOG000142088.
InParanoidiP40453.
KOiK11871.
OMAiGENAWDC.
OrthoDBiEOG7R2BSX.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR001763. Rhodanese-like_dom.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00581. Rhodanese. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40453-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLDDDKGTAM HPHITPFTPE YSNELLRRVQ DLYHEDIKHY YPQLKLEKLL
60 70 80 90 100
DLLEHTEYLF ELYLDSIHHD RPNDALTAFI IGCYYVFLII PQSLQFQTRN
110 120 130 140 150
KSYSIYTDLK KMYENEMNMT NVVLMVKKEI GVVLDESVKH GAGIEHRITK
160 170 180 190 200
KRAFSVPADD LSGQVASLSL DTAAPQDHGL KGTFTEDDAE QSSPVWTAPN
210 220 230 240 250
LEPNDQLKLA LLPEVIPTPA FREPERKTSV PVRPSVLLED VPSIYHEDDT
260 270 280 290 300
SFASLNPPFR EITADRSVTH RKDSYHSVYM VDSGNLKEDN DDLFNVENDG
310 320 330 340 350
FIQSLDILQK QSIITAPELF SILSNRVERE KVLLIDLRIP QRSAINHIVA
360 370 380 390 400
PNLVNVDPNL LWDKQTNTPI YKDDILEHLL KENENFINRN KFDYIVYYTD
410 420 430 440 450
VKTFMTINFD YAFIFFYLML TSQKTPLTTV PTTLLGGYEK WKKTLHSYAQ
460 470 480 490 500
EYHISIEDYL YRPYSQKARL QQEQQQQQQQ PDSQDSFSAK ESSTKVPEPP
510 520 530 540 550
SWKPPDLPIR LRKRPPPPPP VSMPTTPEIP PPLPPKIMVH SQVSSISRKP
560 570 580 590 600
PIPAKQHVKK EQLNSNEIIQ RKRQHQHQHY DQQILQPQRA YNIPTIERSP
610 620 630 640 650
NVYVSLSITG LRNLGNTCYI NSMIQCLFAA KTFRTLFISS KYKSYLQPIR
660 670 680 690 700
SNGSHYSPKL SNSLSMLFNK MYLNGGCSVV PTGFLKVINQ LRPDLKIPDD
710 720 730 740 750
QQDTQEFLMI LLDRLHDELS DQQHVANDYP NLLLYNADAL KVSNNEYKHW
760 770 780 790 800
FDKNVIGNGI SPIDDIFQGQ MENSLQCKRC GYTTFNYSTF YVLSLAIPRR
810 820 830 840 850
SMKLSKLGRS TEKRVKLEDC INMFTSDEVL SGENAWDCPR CGPTASVSTS
860 870 880 890 900
VSALENEPSI VKSKKKKSRF FTLHTGTKRR HLDFFGDGIT EGHNSNNNNT
910 920 930 940 950
TIFERERSRS PFRMLGGSGK RSSSSTPFST GGNDSNNSSD YKNKKLTTVK
960 970 980 990 1000
TINFVTLPKI LVIHLSRFYY DLTKKNNTVV TYPLILNIIL KNNDTMKYKL
1010 1020 1030 1040 1050
FGVVNHTGTL ISGHYTSLVN KDLEHNVNIG RSKWYYFDDE VVKADRKHGS
1060 1070
DKNLKISSSD VYVLFYERVY D
Length:1,071
Mass (Da):123,134
Last modified:February 1, 1995 - v1
Checksum:i82683A01063CEC74
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38059 Genomic DNA. Translation: CAA86122.1.
BK006942 Genomic DNA. Translation: DAA08397.1.
PIRiS48378.
RefSeqiNP_012110.1. NM_001179504.1.

Genome annotation databases

EnsemblFungiiYIL156W; YIL156W; YIL156W.
GeneIDi854650.
KEGGisce:YIL156W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38059 Genomic DNA. Translation: CAA86122.1.
BK006942 Genomic DNA. Translation: DAA08397.1.
PIRiS48378.
RefSeqiNP_012110.1. NM_001179504.1.

3D structure databases

ProteinModelPortaliP40453.
SMRiP40453. Positions 593-884, 943-1071.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34836. 53 interactions.
DIPiDIP-6272N.
IntActiP40453. 13 interactions.
MINTiMINT-598347.

Protein family/group databases

MEROPSiC19.101.

Proteomic databases

MaxQBiP40453.
PaxDbiP40453.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL156W; YIL156W; YIL156W.
GeneIDi854650.
KEGGisce:YIL156W.

Organism-specific databases

CYGDiYIL156w.
EuPathDBiFungiDB:YIL156W.
SGDiS000001418. UBP7.

Phylogenomic databases

eggNOGiCOG5533.
GeneTreeiENSGT00670000099202.
HOGENOMiHOG000142088.
InParanoidiP40453.
KOiK11871.
OMAiGENAWDC.
OrthoDBiEOG7R2BSX.

Enzyme and pathway databases

BioCyciYEAST:G3O-31404-MONOMER.

Miscellaneous databases

NextBioi977199.
PROiP40453.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR001763. Rhodanese-like_dom.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00581. Rhodanese. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Hse1, a component of the yeast Hrs-STAM ubiquitin-sorting complex, associates with ubiquitin peptidases and a ligase to control sorting efficiency into multivesicular bodies."
    Ren J., Kee Y., Huibregtse J.M., Piper R.C.
    Mol. Biol. Cell 18:324-335(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH HSE1.
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiUBP7_YEAST
AccessioniPrimary (citable) accession number: P40453
Secondary accession number(s): D6VVD1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 22, 2015
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 606 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.