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P40453 (UBP7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 7

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 7
Ubiquitin thioesterase 7
Ubiquitin-specific-processing protease 7
Gene names
Name:UBP7
Ordered Locus Names:YIL156W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1071 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the sorting of ubiquitinated cargo proteins at the multivesicular body (MVB). Ref.5

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subcellular location

Cytoplasm Ref.3.

Miscellaneous

Present with 606 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 USP domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10711071Ubiquitin carboxyl-terminal hydrolase 7
PRO_0000080592

Regions

Domain609 – 1069461USP

Sites

Active site6181Nucleophile By similarity
Active site10141Proton acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
P40453 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 82683A01063CEC74

FASTA1,071123,134
        10         20         30         40         50         60 
MLDDDKGTAM HPHITPFTPE YSNELLRRVQ DLYHEDIKHY YPQLKLEKLL DLLEHTEYLF 

        70         80         90        100        110        120 
ELYLDSIHHD RPNDALTAFI IGCYYVFLII PQSLQFQTRN KSYSIYTDLK KMYENEMNMT 

       130        140        150        160        170        180 
NVVLMVKKEI GVVLDESVKH GAGIEHRITK KRAFSVPADD LSGQVASLSL DTAAPQDHGL 

       190        200        210        220        230        240 
KGTFTEDDAE QSSPVWTAPN LEPNDQLKLA LLPEVIPTPA FREPERKTSV PVRPSVLLED 

       250        260        270        280        290        300 
VPSIYHEDDT SFASLNPPFR EITADRSVTH RKDSYHSVYM VDSGNLKEDN DDLFNVENDG 

       310        320        330        340        350        360 
FIQSLDILQK QSIITAPELF SILSNRVERE KVLLIDLRIP QRSAINHIVA PNLVNVDPNL 

       370        380        390        400        410        420 
LWDKQTNTPI YKDDILEHLL KENENFINRN KFDYIVYYTD VKTFMTINFD YAFIFFYLML 

       430        440        450        460        470        480 
TSQKTPLTTV PTTLLGGYEK WKKTLHSYAQ EYHISIEDYL YRPYSQKARL QQEQQQQQQQ 

       490        500        510        520        530        540 
PDSQDSFSAK ESSTKVPEPP SWKPPDLPIR LRKRPPPPPP VSMPTTPEIP PPLPPKIMVH 

       550        560        570        580        590        600 
SQVSSISRKP PIPAKQHVKK EQLNSNEIIQ RKRQHQHQHY DQQILQPQRA YNIPTIERSP 

       610        620        630        640        650        660 
NVYVSLSITG LRNLGNTCYI NSMIQCLFAA KTFRTLFISS KYKSYLQPIR SNGSHYSPKL 

       670        680        690        700        710        720 
SNSLSMLFNK MYLNGGCSVV PTGFLKVINQ LRPDLKIPDD QQDTQEFLMI LLDRLHDELS 

       730        740        750        760        770        780 
DQQHVANDYP NLLLYNADAL KVSNNEYKHW FDKNVIGNGI SPIDDIFQGQ MENSLQCKRC 

       790        800        810        820        830        840 
GYTTFNYSTF YVLSLAIPRR SMKLSKLGRS TEKRVKLEDC INMFTSDEVL SGENAWDCPR 

       850        860        870        880        890        900 
CGPTASVSTS VSALENEPSI VKSKKKKSRF FTLHTGTKRR HLDFFGDGIT EGHNSNNNNT 

       910        920        930        940        950        960 
TIFERERSRS PFRMLGGSGK RSSSSTPFST GGNDSNNSSD YKNKKLTTVK TINFVTLPKI 

       970        980        990       1000       1010       1020 
LVIHLSRFYY DLTKKNNTVV TYPLILNIIL KNNDTMKYKL FGVVNHTGTL ISGHYTSLVN 

      1030       1040       1050       1060       1070 
KDLEHNVNIG RSKWYYFDDE VVKADRKHGS DKNLKISSSD VYVLFYERVY D 

« Hide

References

« Hide 'large scale' references
[1]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX."
Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. expand/collapse author list , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"Hse1, a component of the yeast Hrs-STAM ubiquitin-sorting complex, associates with ubiquitin peptidases and a ligase to control sorting efficiency into multivesicular bodies."
Ren J., Kee Y., Huibregtse J.M., Piper R.C.
Mol. Biol. Cell 18:324-335(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH HSE1.
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z38059 Genomic DNA. Translation: CAA86122.1.
BK006942 Genomic DNA. Translation: DAA08397.1.
PIRS48378.
RefSeqNP_012110.1. NM_001179504.1.

3D structure databases

ProteinModelPortalP40453.
SMRP40453. Positions 593-884, 943-1071.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34836. 51 interactions.
DIPDIP-6272N.
IntActP40453. 13 interactions.
MINTMINT-598347.
STRING4932.YIL156W.

Protein family/group databases

MEROPSC19.101.

Proteomic databases

MaxQBP40453.
PaxDbP40453.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYIL156W; YIL156W; YIL156W.
GeneID854650.
KEGGsce:YIL156W.

Organism-specific databases

CYGDYIL156w.
SGDS000001418. UBP7.

Phylogenomic databases

eggNOGCOG5533.
GeneTreeENSGT00670000099202.
HOGENOMHOG000142088.
KOK11871.
OMAGENAWDC.
OrthoDBEOG7R2BSX.

Enzyme and pathway databases

BioCycYEAST:G3O-31404-MONOMER.

Gene expression databases

GenevestigatorP40453.

Family and domain databases

InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR001763. Rhodanese-like_dom.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF00581. Rhodanese. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMSSF52821. SSF52821. 1 hit.
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977199.

Entry information

Entry nameUBP7_YEAST
AccessionPrimary (citable) accession number: P40453
Secondary accession number(s): D6VVD1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IX

Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries