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P40453

- UBP7_YEAST

UniProt

P40453 - UBP7_YEAST

Protein

Ubiquitin carboxyl-terminal hydrolase 7

Gene

UBP7

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Involved in the sorting of ubiquitinated cargo proteins at the multivesicular body (MVB).1 Publication

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei618 – 6181NucleophilePROSITE-ProRule annotation
    Active sitei1014 – 10141Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. ubiquitin-specific protease activity Source: SGD

    GO - Biological processi

    1. protein deubiquitination Source: SGD
    2. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31404-MONOMER.

    Protein family/group databases

    MEROPSiC19.101.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 7 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 7
    Ubiquitin thioesterase 7
    Ubiquitin-specific-processing protease 7
    Gene namesi
    Name:UBP7
    Ordered Locus Names:YIL156W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    CYGDiYIL156w.
    SGDiS000001418. UBP7.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10711071Ubiquitin carboxyl-terminal hydrolase 7PRO_0000080592Add
    BLAST

    Proteomic databases

    MaxQBiP40453.
    PaxDbiP40453.

    Expressioni

    Gene expression databases

    GenevestigatoriP40453.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BZZ1P388223EBI-19857,EBI-3889
    HOF1Q050802EBI-19857,EBI-5412
    HSE1P387534EBI-19857,EBI-1382
    LSB3P436032EBI-19857,EBI-22980
    MYO3P360063EBI-19857,EBI-11670
    MYO5Q044394EBI-19857,EBI-11687
    PEX13P806672EBI-19857,EBI-13206

    Protein-protein interaction databases

    BioGridi34836. 51 interactions.
    DIPiDIP-6272N.
    IntActiP40453. 13 interactions.
    MINTiMINT-598347.
    STRINGi4932.YIL156W.

    Structurei

    3D structure databases

    ProteinModelPortaliP40453.
    SMRiP40453. Positions 593-884, 943-1071.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini609 – 1069461USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiCOG5533.
    GeneTreeiENSGT00670000099202.
    HOGENOMiHOG000142088.
    KOiK11871.
    OMAiGENAWDC.
    OrthoDBiEOG7R2BSX.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR001763. Rhodanese-like_dom.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00581. Rhodanese. 1 hit.
    PF00443. UCH. 1 hit.
    [Graphical view]
    SMARTiSM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52821. SSF52821. 1 hit.
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40453-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLDDDKGTAM HPHITPFTPE YSNELLRRVQ DLYHEDIKHY YPQLKLEKLL     50
    DLLEHTEYLF ELYLDSIHHD RPNDALTAFI IGCYYVFLII PQSLQFQTRN 100
    KSYSIYTDLK KMYENEMNMT NVVLMVKKEI GVVLDESVKH GAGIEHRITK 150
    KRAFSVPADD LSGQVASLSL DTAAPQDHGL KGTFTEDDAE QSSPVWTAPN 200
    LEPNDQLKLA LLPEVIPTPA FREPERKTSV PVRPSVLLED VPSIYHEDDT 250
    SFASLNPPFR EITADRSVTH RKDSYHSVYM VDSGNLKEDN DDLFNVENDG 300
    FIQSLDILQK QSIITAPELF SILSNRVERE KVLLIDLRIP QRSAINHIVA 350
    PNLVNVDPNL LWDKQTNTPI YKDDILEHLL KENENFINRN KFDYIVYYTD 400
    VKTFMTINFD YAFIFFYLML TSQKTPLTTV PTTLLGGYEK WKKTLHSYAQ 450
    EYHISIEDYL YRPYSQKARL QQEQQQQQQQ PDSQDSFSAK ESSTKVPEPP 500
    SWKPPDLPIR LRKRPPPPPP VSMPTTPEIP PPLPPKIMVH SQVSSISRKP 550
    PIPAKQHVKK EQLNSNEIIQ RKRQHQHQHY DQQILQPQRA YNIPTIERSP 600
    NVYVSLSITG LRNLGNTCYI NSMIQCLFAA KTFRTLFISS KYKSYLQPIR 650
    SNGSHYSPKL SNSLSMLFNK MYLNGGCSVV PTGFLKVINQ LRPDLKIPDD 700
    QQDTQEFLMI LLDRLHDELS DQQHVANDYP NLLLYNADAL KVSNNEYKHW 750
    FDKNVIGNGI SPIDDIFQGQ MENSLQCKRC GYTTFNYSTF YVLSLAIPRR 800
    SMKLSKLGRS TEKRVKLEDC INMFTSDEVL SGENAWDCPR CGPTASVSTS 850
    VSALENEPSI VKSKKKKSRF FTLHTGTKRR HLDFFGDGIT EGHNSNNNNT 900
    TIFERERSRS PFRMLGGSGK RSSSSTPFST GGNDSNNSSD YKNKKLTTVK 950
    TINFVTLPKI LVIHLSRFYY DLTKKNNTVV TYPLILNIIL KNNDTMKYKL 1000
    FGVVNHTGTL ISGHYTSLVN KDLEHNVNIG RSKWYYFDDE VVKADRKHGS 1050
    DKNLKISSSD VYVLFYERVY D 1071
    Length:1,071
    Mass (Da):123,134
    Last modified:February 1, 1995 - v1
    Checksum:i82683A01063CEC74
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z38059 Genomic DNA. Translation: CAA86122.1.
    BK006942 Genomic DNA. Translation: DAA08397.1.
    PIRiS48378.
    RefSeqiNP_012110.1. NM_001179504.1.

    Genome annotation databases

    EnsemblFungiiYIL156W; YIL156W; YIL156W.
    GeneIDi854650.
    KEGGisce:YIL156W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z38059 Genomic DNA. Translation: CAA86122.1 .
    BK006942 Genomic DNA. Translation: DAA08397.1 .
    PIRi S48378.
    RefSeqi NP_012110.1. NM_001179504.1.

    3D structure databases

    ProteinModelPortali P40453.
    SMRi P40453. Positions 593-884, 943-1071.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34836. 51 interactions.
    DIPi DIP-6272N.
    IntActi P40453. 13 interactions.
    MINTi MINT-598347.
    STRINGi 4932.YIL156W.

    Protein family/group databases

    MEROPSi C19.101.

    Proteomic databases

    MaxQBi P40453.
    PaxDbi P40453.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIL156W ; YIL156W ; YIL156W .
    GeneIDi 854650.
    KEGGi sce:YIL156W.

    Organism-specific databases

    CYGDi YIL156w.
    SGDi S000001418. UBP7.

    Phylogenomic databases

    eggNOGi COG5533.
    GeneTreei ENSGT00670000099202.
    HOGENOMi HOG000142088.
    KOi K11871.
    OMAi GENAWDC.
    OrthoDBi EOG7R2BSX.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31404-MONOMER.

    Miscellaneous databases

    NextBioi 977199.

    Gene expression databases

    Genevestigatori P40453.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR001763. Rhodanese-like_dom.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00581. Rhodanese. 1 hit.
    PF00443. UCH. 1 hit.
    [Graphical view ]
    SMARTi SM00450. RHOD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52821. SSF52821. 1 hit.
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "Hse1, a component of the yeast Hrs-STAM ubiquitin-sorting complex, associates with ubiquitin peptidases and a ligase to control sorting efficiency into multivesicular bodies."
      Ren J., Kee Y., Huibregtse J.M., Piper R.C.
      Mol. Biol. Cell 18:324-335(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH HSE1.
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiUBP7_YEAST
    AccessioniPrimary (citable) accession number: P40453
    Secondary accession number(s): D6VVD1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 606 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3