P40452 (COA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome c oxidase assembly protein 1

Gene names

Name:	COA1
Synonyms:	FMP35
Ordered Locus Names:	YIL157C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	197 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for efficient assembly of cytochrome c oxidase in the mitochondrial inner membrane. Involved in a step coupling MSS51-dependent cotranslational insertion of COX1 to the addition of its heme A and copper B cofactors. Ref.6 Ref.7
Subunit structure	Interacts with COX1, COX14, MSS51 and SHY1. Ref.6 Ref.7
Subcellular location	Mitochondrion inner membrane; Single-pass membrane protein Potential Ref.3 Ref.5 Ref.6 Ref.7.
Miscellaneous	Present with 1680 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the COA1 family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial respiratory chain complex IV assembly Inferred from mutant phenotype Ref.7Ref.6. Source: SGD
Cellular_component	integral to mitochondrial inner membrane Inferred from direct assay Ref.7. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 197	197	Cytochrome c oxidase assembly protein 1		PRO_0000202954
Regions
	Topological domain	1 – 73	73	Mitochondrial matrix Potential
	Transmembrane	74 – 94	21	Helical; Potential
	Topological domain	95 – 197	103	Mitochondrial intermembrane Potential

Sequences

	Sequence		Length	Mass (Da)
	P40452 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 2CE6635E5D043C37	FASTA	197	22,044
	10 20 30 40 50 60 MMLRLVTKGL PKVTPSAAKA VLVRGSLLHS FSTSARFNNS VAEDEAKIVL KDKNRPLRID 70 80 90 100 110 120 RELPDPTTER RKRIAGFLLF SVAIGSALSL IFNYEKTESP IISNTLYYIR RSPATKNILG 130 140 150 160 170 180 ESIEFDGIIP WVYGELNSVK GRINITFYIK GDKNVTGTVR LVADRNTHDE EFLIHEWSVT 190 AAGQKIDLLA ENTKTPI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome IX." Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D., Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E., Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C. , Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:84-87(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
[6]	"Coa1 links the Mss51 post-translational function to Cox1 cofactor insertion in cytochrome c oxidase assembly." Pierrel F., Bestwick M.L., Cobine P.A., Khalimonchuk O., Cricco J.A., Winge D.R. EMBO J. 26:4335-4346(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH COX1; COX14; MSS51 AND SHY1.
[7]	"Shy1 couples Cox1 translational regulation to cytochrome c oxidase assembly." Mick D.U., Wagner K., van der Laan M., Frazier A.E., Perschil I., Pawlas M., Meyer H.E., Warscheid B., Rehling P. EMBO J. 26:4347-4358(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, INTERACTION WITH MSS51 AND SHY1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z38059 Genomic DNA. Translation: CAA86121.1. BK006942 Genomic DNA. Translation: DAA08395.1.
PIR	S48377.
RefSeq	NP_012109.1. NM_001179505.1.
3D structure databases
ProteinModelPortal	P40452.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4708N.
MINT	MINT-539339.
STRING	4932.YIL157C.
Proteomic databases
PaxDb	P40452.
PeptideAtlas	P40452.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YIL157C; YIL157C; YIL157C.
GeneID	854649.
KEGG	sce:YIL157C.
Organism-specific databases
CYGD	YIL157c.
SGD	S000001419. YIL157C.
Phylogenomic databases
eggNOG	NOG71901.
HOGENOM	HOG000187652.
OMA	PWISGEM.
OrthoDB	EOG47M57V.
Gene expression databases
Genevestigator	P40452.
GermOnline	YIL157C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR014807. Cyt_oxidase_assembly-1. [Graphical view]
Pfam	PF08695. Coa1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977196.

Entry information

Entry name

COA1_YEAST

Accession

Primary (citable) accession number: P40452
Secondary accession number(s): D6VVC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program