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P40450

- BNR1_YEAST

UniProt

P40450 - BNR1_YEAST

Protein

BNI1-related protein 1

Gene

BNR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    May organize microtubules by mediating spindle positioning and movement in the budding process. Potential target of the RHO family members By similarity.By similarity

    GO - Molecular functioni

    1. profilin binding Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. actin filament bundle assembly Source: SGD
    2. barbed-end actin filament capping Source: SGD
    3. formin-nucleated actin cable assembly Source: SGD
    4. positive regulation of actin cytoskeleton reorganization Source: SGD

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31407-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    BNI1-related protein 1
    Gene namesi
    Name:BNR1
    Ordered Locus Names:YIL159W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IX

    Organism-specific databases

    CYGDiYIL159w.
    SGDiS000001421. BNR1.

    Subcellular locationi

    GO - Cellular componenti

    1. cellular bud neck Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13751375BNI1-related protein 1PRO_0000194900Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei621 – 6211Phosphoserine1 Publication
    Modified residuei751 – 7511Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP40450.
    PaxDbiP40450.

    Expressioni

    Gene expression databases

    GenevestigatoriP40450.

    Interactioni

    Subunit structurei

    Interacts with profilin at the FH1 domain.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    FUS1P117105EBI-3711,EBI-7179
    HOF1Q050805EBI-3711,EBI-5412
    MYO3P360066EBI-3711,EBI-11670
    MYO5Q044397EBI-3711,EBI-11687

    Protein-protein interaction databases

    BioGridi34833. 112 interactions.
    DIPiDIP-817N.
    IntActiP40450. 18 interactions.
    MINTiMINT-606495.
    STRINGi4932.YIL159W.

    Structurei

    3D structure databases

    ProteinModelPortaliP40450.
    SMRiP40450. Positions 870-1281.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini94 – 490397GBD/FH3PROSITE-ProRule annotationAdd
    BLAST
    Domaini659 – 851193FH1Add
    BLAST
    Domaini868 – 1290423FH2PROSITE-ProRule annotationAdd
    BLAST
    Domaini1302 – 133635DADPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili520 – 60182Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi659 – 839181Pro-richAdd
    BLAST
    Compositional biasi1332 – 13354Arg/Lys-rich (basic)

    Domaini

    The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments By similarity.By similarity

    Sequence similaritiesi

    Belongs to the formin homology family. BNI1 subfamily.Curated
    Contains 1 DAD (diaphanous autoregulatory) domain.PROSITE-ProRule annotation
    Contains 1 FH2 (formin homology 2) domain.PROSITE-ProRule annotation
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG281286.
    GeneTreeiENSGT00710000107854.
    HOGENOMiHOG000095249.
    KOiK17971.
    OMAiCAKENIE.
    OrthoDBiEOG7G4QPH.

    Family and domain databases

    InterProiIPR016024. ARM-type_fold.
    IPR014767. Diaphanous_autoregulatory.
    IPR015425. FH2_Formin.
    IPR014768. GTPase-bd/formin_homology_3.
    [Graphical view]
    PfamiPF02181. FH2. 1 hit.
    [Graphical view]
    SMARTiSM00498. FH2. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.
    PROSITEiPS51231. DAD. 1 hit.
    PS51444. FH2. 1 hit.
    PS51232. GBD_FH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40450-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDSSPNKKTY RYPRRSLSLH ARDRVSEARK LEELNLNDGL VAAGLQLVGV     50
    ALEKQGTGSH IYMKQKNFSA NDVSSSPMVS EEVNGSEMDF NPKCMPQDAS 100
    LVERMFDELL KDGTFFWGAA YKNLQNISLR RKWLLICKIR SSNHWGKKKV 150
    TSSTTYSTHL ATNELAENAH FLDGLVRNLS TGGMKLSKAL YKLEKFLRKQ 200
    SFLQLFLKDE IYLTTLIEKT LPLISKELQF VYLRCFKILM NNPLARIRAL 250
    HSEPLIRWFT ELLTDQNSNL KCQLLSMELL LLLTYVEGST GCELIWDQLS 300
    ILFTDWLEWF DKILADDIAI HSSLYLNWNQ LKIDYSTTFL LLINSILQGF 350
    NNKTALEILN FLKKNNIHNT ITFLELAYKD DPNSVVIMEQ IKQFKSKESA 400
    IFDSMIKTTN DTNSLHPTKD IARIESEPLC LENCLLLKAK DSPVEAPINE 450
    IIQSLWKILD SQKPYSESIK LLKLINSLLF YLIDSFQVST NPSFDETLES 500
    AENVDYVFQD SVNKLLDSLQ SDEIARRAVT EIDDLNAKIS HLNEKLNLVE 550
    NHDKDHLIAK LDESESLISL KTKEIENLKL QLKATKKRLD QITTHQRLYD 600
    QPPSLASSNL SIAGSIIKNN SHGNIIFQNL AKKQQQQQKI SLPKRSTSLL 650
    KSKRVTSLSS YLTDANNENE SQNESEDKSK DSLFQRSTST INFNIPSMKN 700
    ITNMQNVSLN SILSELEFSN SLGTQPNYQS SPVLSSVSSS PKLFPRLSSD 750
    SLDNGIQLVP EVVKLPQLPP PPPPPPPPPL PQSLLTEAEA KPDGVSCIAA 800
    PAPPPLPDLF KTKTCGAVPP PPPPPPLPES LSMNKGPSNH DLVTPPAPPL 850
    PNGLLSSSSV SINPTTTDLK PPPTEKRLKQ IHWDKVEDIK DTLWEDTFQR 900
    QETIKELQTD GIFSQIEDIF KMKSPTKIAN KRNAESSIAL SSNNGKSSNE 950
    LKKISFLSRD LAQQFGINLH MFSQLSDMEF VMKVLNCDND IVQNVNILKF 1000
    FCKEELVNIP KSMLNKYEPY SQGKDGKAVS DLQRADRIFL ELCINLRFYW 1050
    NARSKSLLTL STYERDYYDL IFKLQKIDDA ISHLNRSPKF KSLMFIITEI 1100
    GNHMNKRIVK GIKLKSLTKL AFVRSSIDQN VSFLHFIEKV IRIKYPDIYG 1150
    FVDDLKNIED LGKISLEHVE SECHEFHKKI EDLVTQFQVG KLSKEENLDP 1200
    RDQIIKKVKF KINRAKTKSE LLIGQCKLTL IDLNKLMKYY GEDPKDKESK 1250
    NEFFQPFIEF LAMFKKCAKE NIEKEEMERV YEQRKSLLDM RTSSNKKSNG 1300
    SDENDGEKVN RDAVDLLISK LREVKKDPEP LRRRKSTKLN EIAINVHEGD 1350
    VKTRKDEDHV LLERTHAMLN DIQNI 1375
    Length:1,375
    Mass (Da):156,852
    Last modified:February 1, 1995 - v1
    Checksum:iB16B04A33C9D2F08
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z38059 Genomic DNA. Translation: CAA86119.1.
    BK006942 Genomic DNA. Translation: DAA08393.1.
    PIRiS48375.
    RefSeqiNP_012107.1. NM_001179507.1.

    Genome annotation databases

    EnsemblFungiiYIL159W; YIL159W; YIL159W.
    GeneIDi854647.
    KEGGisce:YIL159W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z38059 Genomic DNA. Translation: CAA86119.1 .
    BK006942 Genomic DNA. Translation: DAA08393.1 .
    PIRi S48375.
    RefSeqi NP_012107.1. NM_001179507.1.

    3D structure databases

    ProteinModelPortali P40450.
    SMRi P40450. Positions 870-1281.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 34833. 112 interactions.
    DIPi DIP-817N.
    IntActi P40450. 18 interactions.
    MINTi MINT-606495.
    STRINGi 4932.YIL159W.

    Proteomic databases

    MaxQBi P40450.
    PaxDbi P40450.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YIL159W ; YIL159W ; YIL159W .
    GeneIDi 854647.
    KEGGi sce:YIL159W.

    Organism-specific databases

    CYGDi YIL159w.
    SGDi S000001421. BNR1.

    Phylogenomic databases

    eggNOGi NOG281286.
    GeneTreei ENSGT00710000107854.
    HOGENOMi HOG000095249.
    KOi K17971.
    OMAi CAKENIE.
    OrthoDBi EOG7G4QPH.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31407-MONOMER.

    Miscellaneous databases

    NextBioi 977190.

    Gene expression databases

    Genevestigatori P40450.

    Family and domain databases

    InterProi IPR016024. ARM-type_fold.
    IPR014767. Diaphanous_autoregulatory.
    IPR015425. FH2_Formin.
    IPR014768. GTPase-bd/formin_homology_3.
    [Graphical view ]
    Pfami PF02181. FH2. 1 hit.
    [Graphical view ]
    SMARTi SM00498. FH2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    PROSITEi PS51231. DAD. 1 hit.
    PS51444. FH2. 1 hit.
    PS51232. GBD_FH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Bni1p and Bnr1p: downstream targets of the Rho family small G-proteins which interact with profilin and regulate actin cytoskeleton in Saccharomyces cerevisiae."
      Imamura H., Tanaka K., Hihara T., Umikawa M., Kamei T., Takahashi K., Sasaki T., Takai Y.
      EMBO J. 16:2745-2755(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-621 AND SER-751, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiBNR1_YEAST
    AccessioniPrimary (citable) accession number: P40450
    Secondary accession number(s): D6VVC7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 259 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IX
      Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

    External Data

    Dasty 3