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P40450

- BNR1_YEAST

UniProt

P40450 - BNR1_YEAST

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Protein

BNI1-related protein 1

Gene

BNR1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May organize microtubules by mediating spindle positioning and movement in the budding process. Potential target of the RHO family members (By similarity).By similarity

GO - Molecular functioni

  1. profilin binding Source: SGD

GO - Biological processi

  1. actin filament bundle assembly Source: SGD
  2. barbed-end actin filament capping Source: SGD
  3. formin-nucleated actin cable assembly Source: SGD
  4. positive regulation of actin cytoskeleton reorganization Source: SGD
Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-31407-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
BNI1-related protein 1
Gene namesi
Name:BNR1
Ordered Locus Names:YIL159W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IX

Organism-specific databases

CYGDiYIL159w.
SGDiS000001421. BNR1.

Subcellular locationi

GO - Cellular componenti

  1. cellular bud neck Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13751375BNI1-related protein 1PRO_0000194900Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei621 – 6211Phosphoserine1 Publication
Modified residuei751 – 7511Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40450.
PaxDbiP40450.

Expressioni

Gene expression databases

GenevestigatoriP40450.

Interactioni

Subunit structurei

Interacts with profilin at the FH1 domain.

Binary interactionsi

WithEntry#Exp.IntActNotes
FUS1P117105EBI-3711,EBI-7179
HOF1Q050805EBI-3711,EBI-5412
MYO3P360066EBI-3711,EBI-11670
MYO5Q044397EBI-3711,EBI-11687

Protein-protein interaction databases

BioGridi34833. 112 interactions.
DIPiDIP-817N.
IntActiP40450. 18 interactions.
MINTiMINT-606495.
STRINGi4932.YIL159W.

Structurei

3D structure databases

ProteinModelPortaliP40450.
SMRiP40450. Positions 870-1281.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini94 – 490397GBD/FH3PROSITE-ProRule annotationAdd
BLAST
Domaini659 – 851193FH1Add
BLAST
Domaini868 – 1290423FH2PROSITE-ProRule annotationAdd
BLAST
Domaini1302 – 133635DADPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili520 – 60182Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi659 – 839181Pro-richAdd
BLAST
Compositional biasi1332 – 13354Arg/Lys-rich (basic)

Domaini

The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments (By similarity).By similarity

Sequence similaritiesi

Belongs to the formin homology family. BNI1 subfamily.Curated
Contains 1 DAD (diaphanous autoregulatory) domain.PROSITE-ProRule annotation
Contains 1 FH2 (formin homology 2) domain.PROSITE-ProRule annotation
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG281286.
GeneTreeiENSGT00710000107854.
HOGENOMiHOG000095249.
InParanoidiP40450.
KOiK17971.
OMAiCAKENIE.
OrthoDBiEOG7G4QPH.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR014767. Diaphanous_autoregulatory.
IPR015425. FH2_Formin.
IPR014768. GTPase-bd/formin_homology_3.
[Graphical view]
PfamiPF02181. FH2. 1 hit.
[Graphical view]
SMARTiSM00498. FH2. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS51231. DAD. 1 hit.
PS51444. FH2. 1 hit.
PS51232. GBD_FH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40450 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDSSPNKKTY RYPRRSLSLH ARDRVSEARK LEELNLNDGL VAAGLQLVGV
60 70 80 90 100
ALEKQGTGSH IYMKQKNFSA NDVSSSPMVS EEVNGSEMDF NPKCMPQDAS
110 120 130 140 150
LVERMFDELL KDGTFFWGAA YKNLQNISLR RKWLLICKIR SSNHWGKKKV
160 170 180 190 200
TSSTTYSTHL ATNELAENAH FLDGLVRNLS TGGMKLSKAL YKLEKFLRKQ
210 220 230 240 250
SFLQLFLKDE IYLTTLIEKT LPLISKELQF VYLRCFKILM NNPLARIRAL
260 270 280 290 300
HSEPLIRWFT ELLTDQNSNL KCQLLSMELL LLLTYVEGST GCELIWDQLS
310 320 330 340 350
ILFTDWLEWF DKILADDIAI HSSLYLNWNQ LKIDYSTTFL LLINSILQGF
360 370 380 390 400
NNKTALEILN FLKKNNIHNT ITFLELAYKD DPNSVVIMEQ IKQFKSKESA
410 420 430 440 450
IFDSMIKTTN DTNSLHPTKD IARIESEPLC LENCLLLKAK DSPVEAPINE
460 470 480 490 500
IIQSLWKILD SQKPYSESIK LLKLINSLLF YLIDSFQVST NPSFDETLES
510 520 530 540 550
AENVDYVFQD SVNKLLDSLQ SDEIARRAVT EIDDLNAKIS HLNEKLNLVE
560 570 580 590 600
NHDKDHLIAK LDESESLISL KTKEIENLKL QLKATKKRLD QITTHQRLYD
610 620 630 640 650
QPPSLASSNL SIAGSIIKNN SHGNIIFQNL AKKQQQQQKI SLPKRSTSLL
660 670 680 690 700
KSKRVTSLSS YLTDANNENE SQNESEDKSK DSLFQRSTST INFNIPSMKN
710 720 730 740 750
ITNMQNVSLN SILSELEFSN SLGTQPNYQS SPVLSSVSSS PKLFPRLSSD
760 770 780 790 800
SLDNGIQLVP EVVKLPQLPP PPPPPPPPPL PQSLLTEAEA KPDGVSCIAA
810 820 830 840 850
PAPPPLPDLF KTKTCGAVPP PPPPPPLPES LSMNKGPSNH DLVTPPAPPL
860 870 880 890 900
PNGLLSSSSV SINPTTTDLK PPPTEKRLKQ IHWDKVEDIK DTLWEDTFQR
910 920 930 940 950
QETIKELQTD GIFSQIEDIF KMKSPTKIAN KRNAESSIAL SSNNGKSSNE
960 970 980 990 1000
LKKISFLSRD LAQQFGINLH MFSQLSDMEF VMKVLNCDND IVQNVNILKF
1010 1020 1030 1040 1050
FCKEELVNIP KSMLNKYEPY SQGKDGKAVS DLQRADRIFL ELCINLRFYW
1060 1070 1080 1090 1100
NARSKSLLTL STYERDYYDL IFKLQKIDDA ISHLNRSPKF KSLMFIITEI
1110 1120 1130 1140 1150
GNHMNKRIVK GIKLKSLTKL AFVRSSIDQN VSFLHFIEKV IRIKYPDIYG
1160 1170 1180 1190 1200
FVDDLKNIED LGKISLEHVE SECHEFHKKI EDLVTQFQVG KLSKEENLDP
1210 1220 1230 1240 1250
RDQIIKKVKF KINRAKTKSE LLIGQCKLTL IDLNKLMKYY GEDPKDKESK
1260 1270 1280 1290 1300
NEFFQPFIEF LAMFKKCAKE NIEKEEMERV YEQRKSLLDM RTSSNKKSNG
1310 1320 1330 1340 1350
SDENDGEKVN RDAVDLLISK LREVKKDPEP LRRRKSTKLN EIAINVHEGD
1360 1370
VKTRKDEDHV LLERTHAMLN DIQNI
Length:1,375
Mass (Da):156,852
Last modified:February 1, 1995 - v1
Checksum:iB16B04A33C9D2F08
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z38059 Genomic DNA. Translation: CAA86119.1.
BK006942 Genomic DNA. Translation: DAA08393.1.
PIRiS48375.
RefSeqiNP_012107.1. NM_001179507.1.

Genome annotation databases

EnsemblFungiiYIL159W; YIL159W; YIL159W.
GeneIDi854647.
KEGGisce:YIL159W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z38059 Genomic DNA. Translation: CAA86119.1 .
BK006942 Genomic DNA. Translation: DAA08393.1 .
PIRi S48375.
RefSeqi NP_012107.1. NM_001179507.1.

3D structure databases

ProteinModelPortali P40450.
SMRi P40450. Positions 870-1281.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34833. 112 interactions.
DIPi DIP-817N.
IntActi P40450. 18 interactions.
MINTi MINT-606495.
STRINGi 4932.YIL159W.

Proteomic databases

MaxQBi P40450.
PaxDbi P40450.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YIL159W ; YIL159W ; YIL159W .
GeneIDi 854647.
KEGGi sce:YIL159W.

Organism-specific databases

CYGDi YIL159w.
SGDi S000001421. BNR1.

Phylogenomic databases

eggNOGi NOG281286.
GeneTreei ENSGT00710000107854.
HOGENOMi HOG000095249.
InParanoidi P40450.
KOi K17971.
OMAi CAKENIE.
OrthoDBi EOG7G4QPH.

Enzyme and pathway databases

BioCyci YEAST:G3O-31407-MONOMER.

Miscellaneous databases

NextBioi 977190.

Gene expression databases

Genevestigatori P40450.

Family and domain databases

InterProi IPR016024. ARM-type_fold.
IPR014767. Diaphanous_autoregulatory.
IPR015425. FH2_Formin.
IPR014768. GTPase-bd/formin_homology_3.
[Graphical view ]
Pfami PF02181. FH2. 1 hit.
[Graphical view ]
SMARTi SM00498. FH2. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
PROSITEi PS51231. DAD. 1 hit.
PS51444. FH2. 1 hit.
PS51232. GBD_FH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Bni1p and Bnr1p: downstream targets of the Rho family small G-proteins which interact with profilin and regulate actin cytoskeleton in Saccharomyces cerevisiae."
    Imamura H., Tanaka K., Hihara T., Umikawa M., Kamei T., Takahashi K., Sasaki T., Takai Y.
    EMBO J. 16:2745-2755(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-621 AND SER-751, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBNR1_YEAST
AccessioniPrimary (citable) accession number: P40450
Secondary accession number(s): D6VVC7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 259 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

External Data

Dasty 3