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Protein

6-phosphofructo-2-kinase 1

Gene

PFK26

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Synthesis of fructose 2,6-bisphosphate.

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.

Enzyme regulationi

Phosphorylation results in the activation of the kinase activity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei277 – 2771Sequence analysis
Active sitei309 – 3091Sequence analysis
Binding sitei343 – 3431Fructose-6-phosphateBy similarity
Active sitei404 – 4041Phosphoserine intermediateBy similarity
Active sitei497 – 4971Sequence analysis
Active sitei565 – 5651Proton donorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi190 – 1978ATPSequence analysis

GO - Molecular functioni

  • 6-phosphofructo-2-kinase activity Source: SGD
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

  • fructose 2,6-bisphosphate metabolic process Source: SGD
  • fructose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YIL107C-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
6-phosphofructo-2-kinase 1 (EC:2.7.1.105)
Short name:
6PF-2-K 1
Alternative name(s):
Phosphofructokinase 2 I
Gene namesi
Name:PFK26
Ordered Locus Names:YIL107C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IX

Organism-specific databases

EuPathDBiFungiDB:YIL107C.
SGDiS000001369. PFK26.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8278276-phosphofructo-2-kinase 1PRO_0000179976Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei92 – 921PhosphoserineCombined sources
Modified residuei157 – 1571PhosphothreonineCombined sources
Modified residuei644 – 6441PhosphoserineCombined sources
Modified residuei652 – 6521PhosphoserineCombined sources
Modified residuei659 – 6591PhosphoserineCombined sources
Modified residuei667 – 6671PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40433.

PTM databases

iPTMnetiP40433.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SSB1P114842EBI-1956,EBI-8627

Protein-protein interaction databases

BioGridi34884. 51 interactions.
IntActiP40433. 271 interactions.
MINTiMINT-2783505.

Structurei

3D structure databases

ProteinModelPortaliP40433.
SMRiP40433. Positions 136-637.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

GeneTreeiENSGT00550000075979.
HOGENOMiHOG000251753.
InParanoidiP40433.
KOiK00900.
OMAiKPYGITW.
OrthoDBiEOG092C2SJU.

Family and domain databases

CDDicd07067. HP_PGM_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003094. 6Pfruct_kin.
IPR013079. 6Phosfructo_kin.
IPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10606. PTHR10606. 1 hit.
PfamiPF01591. 6PF2K. 1 hit.
PF00300. His_Phos_1. 1 hit.
[Graphical view]
PRINTSiPR00991. 6PFRUCTKNASE.
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF53254. SSF53254. 1 hit.

Sequencei

Sequence statusi: Complete.

P40433-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKPVDFSET SPVPPDIDLA PTQSPHHVAP SQDSSYDLLS RSSDDKIDAE
60 70 80 90 100
KGPHDELSKH LPLFQKRPLS DTPISSNWNS PGITEENTPS DSPENSATNL
110 120 130 140 150
KSLHRLHIND ETQLKNAKIP TNDTTDYMPP SDGANEVTRI DLKDIKSPTR
160 170 180 190 200
HHKRRPTTID VPGLTKSKTS PDGLISKEDS GSKLVIVMVG LPATGKSFIT
210 220 230 240 250
NKLSRFLNYS LYYCKVFNVG NTRRKFAKEH GLKDQDSKFF EPKNADSTRL
260 270 280 290 300
RDKWAMDTLD ELLDYLLEGS GSVGIFDATN TSRERRKNVL ARIRKRSPHL
310 320 330 340 350
KVLFLESVCS DHALVQKNIR LKLFGPDYKG KDPESSLKDF KSRLANYLKA
360 370 380 390 400
YEPIEDDENL QYIKMIDVGK KVIAYNIQGF LASQTVYYLL NFNLADRQIW
410 420 430 440 450
ITRSGESEDN VSGRIGGNSH LTPRGLRFAK SLPKFIARQR EIFYQNLMQQ
460 470 480 490 500
KKNNENTDGN IYNDFFVWTS MRARTIGTAQ YFNEDDYPIK QMKMLDELSA
510 520 530 540 550
GDYDGMTYPE IKNNFPEEFE KRQKDKLRYR YPGIGGESYM DVINRLRPVI
560 570 580 590 600
TELERIEDNV LIITHRVVAR ALLGYFMNLS MGIIANLDVP LHCVYCLEPK
610 620 630 640 650
PYGITWSLWE YDEASDSFSK VPQTDLNTTR VKEVGLVYNE RRYSVIPTAP
660 670 680 690 700
PSARSSFASD FLSRKRSNPT SASSSQSELS EQPKNSVSAQ TGSNNTTLIG
710 720 730 740 750
SNFNIKNENG DSRIPLSAPL MATNTSNNIL DGGGTSISIH RPRVVPNQNN
760 770 780 790 800
VNPLLANNNK AASNVPNVKK SAATPRQIFE IDKVDEKLSM LKNKSFLLHG
810 820
KDYPNNADNN DNEDIRAKTM NRSQSHV
Length:827
Mass (Da):93,417
Last modified:February 1, 1995 - v1
Checksum:i9FA7BCBA08C9F0E3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti382 – 3821A → R in AAA34858 (PubMed:1657152).Curated
Sequence conflicti477 – 4771G → E AA sequence (PubMed:1851090).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38125 Genomic DNA. Translation: CAA86273.1.
M80801 Genomic DNA. Translation: AAA34858.1.
AY692819 Genomic DNA. Translation: AAT92838.1.
BK006942 Genomic DNA. Translation: DAA08446.1.
PIRiS48465.
RefSeqiNP_012159.1. NM_001179455.1.

Genome annotation databases

EnsemblFungiiYIL107C; YIL107C; YIL107C.
GeneIDi854699.
KEGGisce:YIL107C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z38125 Genomic DNA. Translation: CAA86273.1.
M80801 Genomic DNA. Translation: AAA34858.1.
AY692819 Genomic DNA. Translation: AAT92838.1.
BK006942 Genomic DNA. Translation: DAA08446.1.
PIRiS48465.
RefSeqiNP_012159.1. NM_001179455.1.

3D structure databases

ProteinModelPortaliP40433.
SMRiP40433. Positions 136-637.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34884. 51 interactions.
IntActiP40433. 271 interactions.
MINTiMINT-2783505.

PTM databases

iPTMnetiP40433.

Proteomic databases

MaxQBiP40433.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYIL107C; YIL107C; YIL107C.
GeneIDi854699.
KEGGisce:YIL107C.

Organism-specific databases

EuPathDBiFungiDB:YIL107C.
SGDiS000001369. PFK26.

Phylogenomic databases

GeneTreeiENSGT00550000075979.
HOGENOMiHOG000251753.
InParanoidiP40433.
KOiK00900.
OMAiKPYGITW.
OrthoDBiEOG092C2SJU.

Enzyme and pathway databases

BioCyciYEAST:YIL107C-MONOMER.

Miscellaneous databases

PROiP40433.

Family and domain databases

CDDicd07067. HP_PGM_like. 1 hit.
Gene3Di3.40.50.1240. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003094. 6Pfruct_kin.
IPR013079. 6Phosfructo_kin.
IPR013078. His_Pase_superF_clade-1.
IPR029033. His_PPase_superfam.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10606. PTHR10606. 1 hit.
PfamiPF01591. 6PF2K. 1 hit.
PF00300. His_Phos_1. 1 hit.
[Graphical view]
PRINTSiPR00991. 6PFRUCTKNASE.
SMARTiSM00855. PGAM. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF53254. SSF53254. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry namei6P21_YEAST
AccessioniPrimary (citable) accession number: P40433
Secondary accession number(s): D6VVI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 7, 2016
This is version 153 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1710 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome IX
    Yeast (Saccharomyces cerevisiae) chromosome IX: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.