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Protein

Pre-B-cell leukemia transcription factor 2

Gene

PBX2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator that binds the sequence 5'-ATCAATCAA-3'. Activates transcription of PF4 in complex with MEIS1.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi244 – 30663Homeobox; TALE-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-B-cell leukemia transcription factor 2
Alternative name(s):
Homeobox protein PBX2
Protein G17
Gene namesi
Name:PBX2
Synonyms:G17
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componentsi: Chromosome 6, Unplaced

Organism-specific databases

HGNCiHGNC:8633. PBX2.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: UniProtKB
  • transcription factor complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA32971.

Polymorphism and mutation databases

BioMutaiPBX2.
DMDMi1352729.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 430430Pre-B-cell leukemia transcription factor 2PRO_0000049237Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei136 – 1361Phosphoserine1 Publication
Modified residuei151 – 1511Phosphoserine3 Publications
Modified residuei330 – 3301Phosphoserine2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40425.
PaxDbiP40425.
PRIDEiP40425.

PTM databases

PhosphoSiteiP40425.

Expressioni

Tissue specificityi

Ubiquitously expressed.

Gene expression databases

BgeeiP40425.
CleanExiHS_PBX2.
ExpressionAtlasiP40425. baseline and differential.
GenevisibleiP40425. HS.

Organism-specific databases

HPAiHPA061478.

Interactioni

Subunit structurei

Forms heterodimers with MEIS1 and heterotrimers with MEIS1 and HOXA9. Interacts with PBXIP1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CARD9Q9H2573EBI-348489,EBI-751319
HOXA1P496393EBI-348489,EBI-740785

Protein-protein interaction databases

BioGridi111122. 16 interactions.
IntActiP40425. 22 interactions.
MINTiMINT-1032522.
STRINGi9606.ENSP00000364190.

Structurei

3D structure databases

ProteinModelPortaliP40425.
SMRiP40425. Positions 244-316.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi137 – 1459Poly-Ala

Sequence similaritiesi

Belongs to the TALE/PBX homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG248144.
GeneTreeiENSGT00390000016426.
HOVERGENiHBG000122.
InParanoidiP40425.
KOiK15609.
OMAiIRANGGW.
OrthoDBiEOG75XGM1.
PhylomeDBiP40425.
TreeFamiTF314340.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR005542. PBX.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF03792. PBC. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40425-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDERLLGPPP PGGGRGGLGL VSGEPGGPGE PPGGGDPGGG SGGVPGGRGK
60 70 80 90 100
QDIGDILQQI MTITDQSLDE AQAKKHALNC HRMKPALFSV LCEIKEKTGL
110 120 130 140 150
SIRSSQEEEP VDPQLMRLDN MLLAEGVAGP EKGGGSAAAA AAAAASGGGV
160 170 180 190 200
SPDNSIEHSD YRSKLAQIRH IYHSELEKYE QACNEFTTHV MNLLREQSRT
210 220 230 240 250
RPVAPKEMER MVSIIHRKFS AIQMQLKQST CEAVMILRSR FLDARRKRRN
260 270 280 290 300
FSKQATEVLN EYFYSHLSNP YPSEEAKEEL AKKCGITVSQ VSNWFGNKRI
310 320 330 340 350
RYKKNIGKFQ EEANIYAVKT AVSVTQGGHS RTSSPTPPSS AGSGGSFNLS
360 370 380 390 400
GSGDMFLGMP GLNGDSYSAS QVESLRHSMG PGGYGDNLGG GQMYSPREMR
410 420 430
ANGSWQEAVT PSSVTSPTEG PGSVHSDTSN
Length:430
Mass (Da):45,881
Last modified:February 1, 1996 - v2
Checksum:iEF2FFA158C4DAF68
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti393 – 3931M → I in CAA42503 (PubMed:1682799).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59842 mRNA. Translation: CAA42503.1.
X80700 Genomic DNA. Translation: CAA56717.1.
D28769 Genomic DNA. Translation: BAA05957.1.
U89336 Genomic DNA. Translation: AAB47490.1.
AL662884 Genomic DNA. Translation: CAI18358.1.
AL662830 Genomic DNA. Translation: CAI17539.1.
AL845464 Genomic DNA. Translation: CAI41814.1.
BX284686 Genomic DNA. Translation: CAM26228.1.
BX927239 Genomic DNA. Translation: CAQ06600.1.
CR933878 Genomic DNA. Translation: CAQ09629.1.
CR812478 Genomic DNA. Translation: CAQ10704.1.
CH471081 Genomic DNA. Translation: EAX03613.1.
BC082261 mRNA. Translation: AAH82261.1.
CCDSiCCDS4748.1.
PIRiA56002.
RefSeqiNP_002577.2. NM_002586.4.
UniGeneiHs.509545.
Hs.718462.

Genome annotation databases

EnsembliENST00000375050; ENSP00000364190; ENSG00000204304.
ENST00000383270; ENSP00000372757; ENSG00000206315.
ENST00000420432; ENSP00000410710; ENSG00000236353.
ENST00000431537; ENSP00000390009; ENSG00000232005.
ENST00000433552; ENSP00000391745; ENSG00000224952.
ENST00000453487; ENSP00000403948; ENSG00000225987.
ENST00000453961; ENSP00000397177; ENSG00000237344.
GeneIDi5089.
KEGGihsa:5089.
UCSCiuc003oav.1. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59842 mRNA. Translation: CAA42503.1.
X80700 Genomic DNA. Translation: CAA56717.1.
D28769 Genomic DNA. Translation: BAA05957.1.
U89336 Genomic DNA. Translation: AAB47490.1.
AL662884 Genomic DNA. Translation: CAI18358.1.
AL662830 Genomic DNA. Translation: CAI17539.1.
AL845464 Genomic DNA. Translation: CAI41814.1.
BX284686 Genomic DNA. Translation: CAM26228.1.
BX927239 Genomic DNA. Translation: CAQ06600.1.
CR933878 Genomic DNA. Translation: CAQ09629.1.
CR812478 Genomic DNA. Translation: CAQ10704.1.
CH471081 Genomic DNA. Translation: EAX03613.1.
BC082261 mRNA. Translation: AAH82261.1.
CCDSiCCDS4748.1.
PIRiA56002.
RefSeqiNP_002577.2. NM_002586.4.
UniGeneiHs.509545.
Hs.718462.

3D structure databases

ProteinModelPortaliP40425.
SMRiP40425. Positions 244-316.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111122. 16 interactions.
IntActiP40425. 22 interactions.
MINTiMINT-1032522.
STRINGi9606.ENSP00000364190.

PTM databases

PhosphoSiteiP40425.

Polymorphism and mutation databases

BioMutaiPBX2.
DMDMi1352729.

Proteomic databases

MaxQBiP40425.
PaxDbiP40425.
PRIDEiP40425.

Protocols and materials databases

DNASUi5089.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000375050; ENSP00000364190; ENSG00000204304.
ENST00000383270; ENSP00000372757; ENSG00000206315.
ENST00000420432; ENSP00000410710; ENSG00000236353.
ENST00000431537; ENSP00000390009; ENSG00000232005.
ENST00000433552; ENSP00000391745; ENSG00000224952.
ENST00000453487; ENSP00000403948; ENSG00000225987.
ENST00000453961; ENSP00000397177; ENSG00000237344.
GeneIDi5089.
KEGGihsa:5089.
UCSCiuc003oav.1. human.

Organism-specific databases

CTDi5089.
GeneCardsiGC06M032158.
GC06Mi32163.
GC06Mj32100.
GC06Ml32191.
GC06Mm32228.
GC06Mn32110.
GC06Mo32159.
HGNCiHGNC:8633. PBX2.
HPAiHPA061478.
MIMi176311. gene.
neXtProtiNX_P40425.
PharmGKBiPA32971.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG248144.
GeneTreeiENSGT00390000016426.
HOVERGENiHBG000122.
InParanoidiP40425.
KOiK15609.
OMAiIRANGGW.
OrthoDBiEOG75XGM1.
PhylomeDBiP40425.
TreeFamiTF314340.

Miscellaneous databases

ChiTaRSiPBX2. human.
GeneWikiiPBX2.
GenomeRNAii5089.
NextBioi19624.
PROiP40425.
SOURCEiSearch...

Gene expression databases

BgeeiP40425.
CleanExiHS_PBX2.
ExpressionAtlasiP40425. baseline and differential.
GenevisibleiP40425. HS.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR005542. PBX.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF03792. PBC. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "PBX2 and PBX3, new homeobox genes with extensive homology to the human proto-oncogene PBX1."
    Monica K., Galili N., Nourse J., Saltman D., Cleary M.L.
    Mol. Cell. Biol. 11:6149-6157(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The novel gene G17, located in the human major histocompatibility complex, encodes PBX2, a homeodomain-containing protein."
    Aguado B., Campbell R.D.
    Genomics 25:650-659(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Three genes in the human MHC class III region near the junction with the class II: gene for receptor of advanced glycosylation end products, PBX2 homeobox gene and a notch homolog, human counterpart of mouse mammary tumor gene int-3."
    Sugaya K., Fukagawa T., Matsumoto K., Mita K., Takahashi E., Ando A., Inoko H., Ikemura T.
    Genomics 23:408-419(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse."
    Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D., Hood L.
    Genome Res. 13:2621-2636(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  8. "Fusion with E2A converts the Pbx1 homeodomain protein into a constitutive transcriptional activator in human leukemias carrying the t(1;19) translocation."
    Lu Q., Wright D.D., Kamps M.P.
    Mol. Cell. Biol. 14:3938-3948(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  9. "Functional cloning and characterization of a novel nonhomeodomain protein that inhibits the binding of PBX1-HOX complexes to DNA."
    Abramovich C., Shen W.-F., Pineault N., Imren S., Montpetit B., Largman C., Humphries R.K.
    J. Biol. Chem. 275:26172-26177(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PBXIP1.
  10. "Homeodomain proteins MEIS1 and PBXs regulate the lineage-specific transcription of the platelet factor 4 gene."
    Okada Y., Nagai R., Sato T., Matsuura E., Minami T., Morita I., Doi T.
    Blood 101:4748-4756(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MEIS1.
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic kidney.
  12. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
    Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
    J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-151, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  13. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-151 AND SER-330, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  14. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-151, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  15. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  16. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-330, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  17. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-136, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiPBX2_HUMAN
AccessioniPrimary (citable) accession number: P40425
Secondary accession number(s): A2BFJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1996
Last modified: June 24, 2015
This is version 145 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.