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P40425

- PBX2_HUMAN

UniProt

P40425 - PBX2_HUMAN

Protein

Pre-B-cell leukemia transcription factor 2

Gene

PBX2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Transcriptional activator that binds the sequence 5'-ATCAATCAA-3'. Activates transcription of PF4 in complex with MEIS1.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi244 – 30663Homeobox; TALE-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: Ensembl
    2. sequence-specific DNA binding Source: Ensembl
    3. sequence-specific DNA binding transcription factor activity Source: Ensembl
    4. transcription factor binding Source: UniProtKB

    GO - Biological processi

    1. embryonic limb morphogenesis Source: Ensembl
    2. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
    3. proximal/distal pattern formation Source: Ensembl
    4. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pre-B-cell leukemia transcription factor 2
    Alternative name(s):
    Homeobox protein PBX2
    Protein G17
    Gene namesi
    Name:PBX2
    Synonyms:G17
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 6

    Organism-specific databases

    HGNCiHGNC:8633. PBX2.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB
    2. transcription factor complex Source: Ensembl

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA32971.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 430430Pre-B-cell leukemia transcription factor 2PRO_0000049237Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei151 – 1511Phosphoserine3 Publications
    Modified residuei330 – 3301Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP40425.
    PaxDbiP40425.
    PRIDEiP40425.

    PTM databases

    PhosphoSiteiP40425.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed.

    Gene expression databases

    BgeeiP40425.
    CleanExiHS_PBX2.
    GenevestigatoriP40425.

    Organism-specific databases

    HPAiHPA054000.
    HPA061478.

    Interactioni

    Subunit structurei

    Forms heterodimers with MEIS1 and heterotrimers with MEIS1 and HOXA9. Interacts with PBXIP1.2 Publications

    Protein-protein interaction databases

    BioGridi111122. 3 interactions.
    IntActiP40425. 20 interactions.
    MINTiMINT-1032522.
    STRINGi9606.ENSP00000397177.

    Structurei

    3D structure databases

    ProteinModelPortaliP40425.
    SMRiP40425. Positions 244-316.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi137 – 1459Poly-Ala

    Sequence similaritiesi

    Belongs to the TALE/PBX homeobox family.Curated
    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    eggNOGiNOG248144.
    HOVERGENiHBG000122.
    InParanoidiP40425.
    KOiK15609.
    OMAiGQIYSPR.
    OrthoDBiEOG75XGM1.
    PhylomeDBiP40425.
    TreeFamiTF314340.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR005542. PBX.
    [Graphical view]
    PfamiPF00046. Homeobox. 1 hit.
    PF03792. PBC. 1 hit.
    [Graphical view]
    SMARTiSM00389. HOX. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40425-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDERLLGPPP PGGGRGGLGL VSGEPGGPGE PPGGGDPGGG SGGVPGGRGK    50
    QDIGDILQQI MTITDQSLDE AQAKKHALNC HRMKPALFSV LCEIKEKTGL 100
    SIRSSQEEEP VDPQLMRLDN MLLAEGVAGP EKGGGSAAAA AAAAASGGGV 150
    SPDNSIEHSD YRSKLAQIRH IYHSELEKYE QACNEFTTHV MNLLREQSRT 200
    RPVAPKEMER MVSIIHRKFS AIQMQLKQST CEAVMILRSR FLDARRKRRN 250
    FSKQATEVLN EYFYSHLSNP YPSEEAKEEL AKKCGITVSQ VSNWFGNKRI 300
    RYKKNIGKFQ EEANIYAVKT AVSVTQGGHS RTSSPTPPSS AGSGGSFNLS 350
    GSGDMFLGMP GLNGDSYSAS QVESLRHSMG PGGYGDNLGG GQMYSPREMR 400
    ANGSWQEAVT PSSVTSPTEG PGSVHSDTSN 430
    Length:430
    Mass (Da):45,881
    Last modified:February 1, 1996 - v2
    Checksum:iEF2FFA158C4DAF68
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti393 – 3931M → I in CAA42503. (PubMed:1682799)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59842 mRNA. Translation: CAA42503.1.
    X80700 Genomic DNA. Translation: CAA56717.1.
    D28769 Genomic DNA. Translation: BAA05957.1.
    U89336 Genomic DNA. Translation: AAB47490.1.
    AL662884 Genomic DNA. Translation: CAI18358.1.
    AL662830 Genomic DNA. Translation: CAI17539.1.
    AL845464 Genomic DNA. Translation: CAI41814.1.
    BX284686 Genomic DNA. Translation: CAM26228.1.
    BX927239 Genomic DNA. Translation: CAQ06600.1.
    CR933878 Genomic DNA. Translation: CAQ09629.1.
    CR812478 Genomic DNA. Translation: CAQ10704.1.
    CH471081 Genomic DNA. Translation: EAX03613.1.
    BC082261 mRNA. Translation: AAH82261.1.
    CCDSiCCDS4748.1.
    PIRiA56002.
    RefSeqiNP_002577.2. NM_002586.4.
    UniGeneiHs.509545.
    Hs.718462.

    Genome annotation databases

    EnsembliENST00000375050; ENSP00000364190; ENSG00000204304.
    ENST00000383270; ENSP00000372757; ENSG00000206315.
    ENST00000420432; ENSP00000410710; ENSG00000236353.
    ENST00000431537; ENSP00000390009; ENSG00000232005.
    ENST00000433552; ENSP00000391745; ENSG00000224952.
    ENST00000453487; ENSP00000403948; ENSG00000225987.
    ENST00000453961; ENSP00000397177; ENSG00000237344.
    GeneIDi5089.
    KEGGihsa:5089.
    UCSCiuc003oav.1. human.

    Polymorphism databases

    DMDMi1352729.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59842 mRNA. Translation: CAA42503.1 .
    X80700 Genomic DNA. Translation: CAA56717.1 .
    D28769 Genomic DNA. Translation: BAA05957.1 .
    U89336 Genomic DNA. Translation: AAB47490.1 .
    AL662884 Genomic DNA. Translation: CAI18358.1 .
    AL662830 Genomic DNA. Translation: CAI17539.1 .
    AL845464 Genomic DNA. Translation: CAI41814.1 .
    BX284686 Genomic DNA. Translation: CAM26228.1 .
    BX927239 Genomic DNA. Translation: CAQ06600.1 .
    CR933878 Genomic DNA. Translation: CAQ09629.1 .
    CR812478 Genomic DNA. Translation: CAQ10704.1 .
    CH471081 Genomic DNA. Translation: EAX03613.1 .
    BC082261 mRNA. Translation: AAH82261.1 .
    CCDSi CCDS4748.1.
    PIRi A56002.
    RefSeqi NP_002577.2. NM_002586.4.
    UniGenei Hs.509545.
    Hs.718462.

    3D structure databases

    ProteinModelPortali P40425.
    SMRi P40425. Positions 244-316.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111122. 3 interactions.
    IntActi P40425. 20 interactions.
    MINTi MINT-1032522.
    STRINGi 9606.ENSP00000397177.

    PTM databases

    PhosphoSitei P40425.

    Polymorphism databases

    DMDMi 1352729.

    Proteomic databases

    MaxQBi P40425.
    PaxDbi P40425.
    PRIDEi P40425.

    Protocols and materials databases

    DNASUi 5089.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000375050 ; ENSP00000364190 ; ENSG00000204304 .
    ENST00000383270 ; ENSP00000372757 ; ENSG00000206315 .
    ENST00000420432 ; ENSP00000410710 ; ENSG00000236353 .
    ENST00000431537 ; ENSP00000390009 ; ENSG00000232005 .
    ENST00000433552 ; ENSP00000391745 ; ENSG00000224952 .
    ENST00000453487 ; ENSP00000403948 ; ENSG00000225987 .
    ENST00000453961 ; ENSP00000397177 ; ENSG00000237344 .
    GeneIDi 5089.
    KEGGi hsa:5089.
    UCSCi uc003oav.1. human.

    Organism-specific databases

    CTDi 5089.
    GeneCardsi GC06M032153.
    GC06Mi32163.
    GC06Mj32100.
    GC06Ml32191.
    GC06Mm32228.
    GC06Mn32110.
    GC06Mo32159.
    HGNCi HGNC:8633. PBX2.
    HPAi HPA054000.
    HPA061478.
    MIMi 176311. gene.
    neXtProti NX_P40425.
    PharmGKBi PA32971.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG248144.
    HOVERGENi HBG000122.
    InParanoidi P40425.
    KOi K15609.
    OMAi GQIYSPR.
    OrthoDBi EOG75XGM1.
    PhylomeDBi P40425.
    TreeFami TF314340.

    Miscellaneous databases

    ChiTaRSi PBX2. human.
    GeneWikii PBX2.
    GenomeRNAii 5089.
    NextBioi 19624.
    PROi P40425.
    SOURCEi Search...

    Gene expression databases

    Bgeei P40425.
    CleanExi HS_PBX2.
    Genevestigatori P40425.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR005542. PBX.
    [Graphical view ]
    Pfami PF00046. Homeobox. 1 hit.
    PF03792. PBC. 1 hit.
    [Graphical view ]
    SMARTi SM00389. HOX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "PBX2 and PBX3, new homeobox genes with extensive homology to the human proto-oncogene PBX1."
      Monica K., Galili N., Nourse J., Saltman D., Cleary M.L.
      Mol. Cell. Biol. 11:6149-6157(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The novel gene G17, located in the human major histocompatibility complex, encodes PBX2, a homeodomain-containing protein."
      Aguado B., Campbell R.D.
      Genomics 25:650-659(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Three genes in the human MHC class III region near the junction with the class II: gene for receptor of advanced glycosylation end products, PBX2 homeobox gene and a notch homolog, human counterpart of mouse mammary tumor gene int-3."
      Sugaya K., Fukagawa T., Matsumoto K., Mita K., Takahashi E., Ando A., Inoko H., Ikemura T.
      Genomics 23:408-419(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse."
      Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D., Hood L.
      Genome Res. 13:2621-2636(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The DNA sequence and analysis of human chromosome 6."
      Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
      , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
      Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung.
    8. "Fusion with E2A converts the Pbx1 homeodomain protein into a constitutive transcriptional activator in human leukemias carrying the t(1;19) translocation."
      Lu Q., Wright D.D., Kamps M.P.
      Mol. Cell. Biol. 14:3938-3948(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    9. "Functional cloning and characterization of a novel nonhomeodomain protein that inhibits the binding of PBX1-HOX complexes to DNA."
      Abramovich C., Shen W.-F., Pineault N., Imren S., Montpetit B., Largman C., Humphries R.K.
      J. Biol. Chem. 275:26172-26177(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PBXIP1.
    10. "Homeodomain proteins MEIS1 and PBXs regulate the lineage-specific transcription of the platelet factor 4 gene."
      Okada Y., Nagai R., Sato T., Matsuura E., Minami T., Morita I., Doi T.
      Blood 101:4748-4756(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH MEIS1.
    11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic kidney.
    12. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
      Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
      J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-151, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    13. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-151 AND SER-330, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    14. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-151, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    15. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    16. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-330, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPBX2_HUMAN
    AccessioniPrimary (citable) accession number: P40425
    Secondary accession number(s): A2BFJ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 138 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 6
      Human chromosome 6: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3