P40425 (PBX2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pre-B-cell leukemia transcription factor 2

Alternative name(s):
Homeobox protein PBX2
Protein G17

Gene names

Name:	PBX2
Synonyms:	G17

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional activator that binds the sequence 5'-ATCAATCAA-3'. Activates transcription of PF4 in complex with MEIS1. Ref.10
Subunit structure	Forms heterodimers with MEIS1 and heterotrimers with MEIS1 and HOXA9. Interacts with PBXIP1. Ref.9 Ref.10
Subcellular location	Nucleus Probable.
Tissue specificity	Ubiquitously expressed.
Sequence similarities	Belongs to the TALE/PBX homeobox family. Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	embryonic limb morphogenesis Inferred from electronic annotation. Source: Compara positive regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara proximal/distal pattern formation Inferred from electronic annotation. Source: Compara transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from direct assay PubMed 18973687. Source: UniProtKB transcription factor complex Inferred from electronic annotation. Source: Compara
Molecular_function	chromatin binding Inferred from electronic annotation. Source: Compara sequence-specific DNA binding Inferred from electronic annotation. Source: Compara sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 430	430	Pre-B-cell leukemia transcription factor 2		PRO_0000049237
Regions
	DNA binding	244 – 306	63	Homeobox; TALE-type
	Compositional bias	137 – 145	9	Poly-Ala
Amino acid modifications
	Modified residue	151	1	Phosphoserine Ref.12 Ref.13 Ref.14
	Modified residue	330	1	Phosphoserine Ref.13 Ref.16
Experimental info
	Sequence conflict	393	1	M → I in CAA42503. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P40425 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: EF2FFA158C4DAF68	FASTA	430	45,881
	10 20 30 40 50 60 MDERLLGPPP PGGGRGGLGL VSGEPGGPGE PPGGGDPGGG SGGVPGGRGK QDIGDILQQI 70 80 90 100 110 120 MTITDQSLDE AQAKKHALNC HRMKPALFSV LCEIKEKTGL SIRSSQEEEP VDPQLMRLDN 130 140 150 160 170 180 MLLAEGVAGP EKGGGSAAAA AAAAASGGGV SPDNSIEHSD YRSKLAQIRH IYHSELEKYE 190 200 210 220 230 240 QACNEFTTHV MNLLREQSRT RPVAPKEMER MVSIIHRKFS AIQMQLKQST CEAVMILRSR 250 260 270 280 290 300 FLDARRKRRN FSKQATEVLN EYFYSHLSNP YPSEEAKEEL AKKCGITVSQ VSNWFGNKRI 310 320 330 340 350 360 RYKKNIGKFQ EEANIYAVKT AVSVTQGGHS RTSSPTPPSS AGSGGSFNLS GSGDMFLGMP 370 380 390 400 410 420 GLNGDSYSAS QVESLRHSMG PGGYGDNLGG GQMYSPREMR ANGSWQEAVT PSSVTSPTEG 430 PGSVHSDTSN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"PBX2 and PBX3, new homeobox genes with extensive homology to the human proto-oncogene PBX1." Monica K., Galili N., Nourse J., Saltman D., Cleary M.L. Mol. Cell. Biol. 11:6149-6157(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The novel gene G17, located in the human major histocompatibility complex, encodes PBX2, a homeodomain-containing protein." Aguado B., Campbell R.D. Genomics 25:650-659(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Three genes in the human MHC class III region near the junction with the class II: gene for receptor of advanced glycosylation end products, PBX2 homeobox gene and a notch homolog, human counterpart of mouse mammary tumor gene int-3." Sugaya K., Fukagawa T., Matsumoto K., Mita K., Takahashi E., Ando A., Inoko H., Ikemura T. Genomics 23:408-419(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse." Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D., Hood L. Genome Res. 13:2621-2636(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[8]	"Fusion with E2A converts the Pbx1 homeodomain protein into a constitutive transcriptional activator in human leukemias carrying the t(1;19) translocation." Lu Q., Wright D.D., Kamps M.P. Mol. Cell. Biol. 14:3938-3948(1994) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[9]	"Functional cloning and characterization of a novel nonhomeodomain protein that inhibits the binding of PBX1-HOX complexes to DNA." Abramovich C., Shen W.-F., Pineault N., Imren S., Montpetit B., Largman C., Humphries R.K. J. Biol. Chem. 275:26172-26177(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PBXIP1.
[10]	"Homeodomain proteins MEIS1 and PBXs regulate the lineage-specific transcription of the platelet factor 4 gene." Okada Y., Nagai R., Sato T., Matsuura E., Minami T., Morita I., Doi T. Blood 101:4748-4756(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH MEIS1.
[11]	"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J. Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Embryonic kidney.
[12]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-151, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[13]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-151 AND SER-330, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[14]	"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis." Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M. Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-151, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[15]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-330, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59842 mRNA. Translation: CAA42503.1. X80700 Genomic DNA. Translation: CAA56717.1. D28769 Genomic DNA. Translation: BAA05957.1. U89336 Genomic DNA. Translation: AAB47490.1. AL662884 Genomic DNA. Translation: CAI18358.1. AL662830 Genomic DNA. Translation: CAI17539.1. AL845464 Genomic DNA. Translation: CAI41814.1. BX284686 Genomic DNA. Translation: CAM26228.1. BX927239 Genomic DNA. Translation: CAQ06600.1. CR933878 Genomic DNA. Translation: CAQ09629.1. CR812478 Genomic DNA. Translation: CAQ10704.1. CH471081 Genomic DNA. Translation: EAX03613.1. BC082261 mRNA. Translation: AAH82261.1.
IPI	IPI00028416.
PIR	A56002.
RefSeq	NP_002577.2. NM_002586.4.
UniGene	Hs.509545. Hs.718462.
3D structure databases
ProteinModelPortal	P40425.
ModBase	Search...
Protein-protein interaction databases
IntAct	P40425. 20 interactions.
MINT	MINT-1032522.
STRING	9606.ENSP00000364190.
PTM databases
PhosphoSite	P40425.
Polymorphism databases
DMDM	1352729.
Proteomic databases
PaxDb	P40425.
PRIDE	P40425.
Protocols and materials databases
DNASU	5089.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000375050; ENSP00000364190; ENSG00000204304. ENST00000383270; ENSP00000372757; ENSG00000206315. ENST00000420432; ENSP00000410710; ENSG00000236353. ENST00000431537; ENSP00000390009; ENSG00000232005. ENST00000433552; ENSP00000391745; ENSG00000224952. ENST00000453487; ENSP00000403948; ENSG00000225987. ENST00000453961; ENSP00000397177; ENSG00000237344.
GeneID	5089.
KEGG	hsa:5089.
UCSC	uc003oav.1. human.
Organism-specific databases
CTD	5089.
GeneCards	GC06M032151.
HGNC	HGNC:8633. PBX2.
MIM	176311. gene.
neXtProt	NX_P40425.
PharmGKB	PA32971.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG248144.
HOVERGEN	HBG000122.
InParanoid	P40425.
KO	K15609.
OMA	YGENLGG.
OrthoDB	EOG45HRXQ.
PhylomeDB	P40425.
Gene expression databases
ArrayExpress	P40425.
Bgee	P40425.
CleanEx	HS_PBX2.
Genevestigator	P40425.
GermOnline	ENSG00000204304. Homo sapiens.
Family and domain databases
Gene3D	1.10.10.60. 1 hit.
InterPro	IPR017970. Homeobox_CS. IPR001356. Homeodomain. IPR009057. Homeodomain-like. IPR005542. PBX. [Graphical view]
Pfam	PF00046. Homeobox. 1 hit. PF03792. PBC. 1 hit. [Graphical view]
SMART	SM00389. HOX. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	PBX2. human.
GenomeRNAi	5089.
NextBio	19624.
SOURCE	Search...

Entry information

Entry name

PBX2_HUMAN

Accession

Primary (citable) accession number: P40425
Secondary accession number(s): A2BFJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 126 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.