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P40425 (PBX2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pre-B-cell leukemia transcription factor 2
Alternative name(s):
Homeobox protein PBX2
Protein G17
Gene names
Name:PBX2
Synonyms:G17
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length430 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transcriptional activator that binds the sequence 5'-ATCAATCAA-3'. Activates transcription of PF4 in complex with MEIS1. Ref.10

Subunit structure

Forms heterodimers with MEIS1 and heterotrimers with MEIS1 and HOXA9. Interacts with PBXIP1. Ref.9 Ref.10

Subcellular location

Nucleus Probable.

Tissue specificity

Ubiquitously expressed.

Sequence similarities

Belongs to the TALE/PBX homeobox family.

Contains 1 homeobox DNA-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 430430Pre-B-cell leukemia transcription factor 2
PRO_0000049237

Regions

DNA binding244 – 30663Homeobox; TALE-type
Compositional bias137 – 1459Poly-Ala

Amino acid modifications

Modified residue1511Phosphoserine Ref.12 Ref.13 Ref.14
Modified residue3301Phosphoserine Ref.13 Ref.16

Experimental info

Sequence conflict3931M → I in CAA42503. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P40425 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: EF2FFA158C4DAF68

FASTA43045,881
        10         20         30         40         50         60 
MDERLLGPPP PGGGRGGLGL VSGEPGGPGE PPGGGDPGGG SGGVPGGRGK QDIGDILQQI 

        70         80         90        100        110        120 
MTITDQSLDE AQAKKHALNC HRMKPALFSV LCEIKEKTGL SIRSSQEEEP VDPQLMRLDN 

       130        140        150        160        170        180 
MLLAEGVAGP EKGGGSAAAA AAAAASGGGV SPDNSIEHSD YRSKLAQIRH IYHSELEKYE 

       190        200        210        220        230        240 
QACNEFTTHV MNLLREQSRT RPVAPKEMER MVSIIHRKFS AIQMQLKQST CEAVMILRSR 

       250        260        270        280        290        300 
FLDARRKRRN FSKQATEVLN EYFYSHLSNP YPSEEAKEEL AKKCGITVSQ VSNWFGNKRI 

       310        320        330        340        350        360 
RYKKNIGKFQ EEANIYAVKT AVSVTQGGHS RTSSPTPPSS AGSGGSFNLS GSGDMFLGMP 

       370        380        390        400        410        420 
GLNGDSYSAS QVESLRHSMG PGGYGDNLGG GQMYSPREMR ANGSWQEAVT PSSVTSPTEG 

       430 
PGSVHSDTSN 

« Hide

References

« Hide 'large scale' references
[1]"PBX2 and PBX3, new homeobox genes with extensive homology to the human proto-oncogene PBX1."
Monica K., Galili N., Nourse J., Saltman D., Cleary M.L.
Mol. Cell. Biol. 11:6149-6157(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The novel gene G17, located in the human major histocompatibility complex, encodes PBX2, a homeodomain-containing protein."
Aguado B., Campbell R.D.
Genomics 25:650-659(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Three genes in the human MHC class III region near the junction with the class II: gene for receptor of advanced glycosylation end products, PBX2 homeobox gene and a notch homolog, human counterpart of mouse mammary tumor gene int-3."
Sugaya K., Fukagawa T., Matsumoto K., Mita K., Takahashi E., Ando A., Inoko H., Ikemura T.
Genomics 23:408-419(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse."
Xie T., Rowen L., Aguado B., Ahearn M.E., Madan A., Qin S., Campbell R.D., Hood L.
Genome Res. 13:2621-2636(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
[8]"Fusion with E2A converts the Pbx1 homeodomain protein into a constitutive transcriptional activator in human leukemias carrying the t(1;19) translocation."
Lu Q., Wright D.D., Kamps M.P.
Mol. Cell. Biol. 14:3938-3948(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[9]"Functional cloning and characterization of a novel nonhomeodomain protein that inhibits the binding of PBX1-HOX complexes to DNA."
Abramovich C., Shen W.-F., Pineault N., Imren S., Montpetit B., Largman C., Humphries R.K.
J. Biol. Chem. 275:26172-26177(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PBXIP1.
[10]"Homeodomain proteins MEIS1 and PBXs regulate the lineage-specific transcription of the platelet factor 4 gene."
Okada Y., Nagai R., Sato T., Matsuura E., Minami T., Morita I., Doi T.
Blood 101:4748-4756(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH MEIS1.
[11]"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.
Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic kidney.
[12]"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-151, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[13]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-151 AND SER-330, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[14]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-151, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[15]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[16]"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-330, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X59842 mRNA. Translation: CAA42503.1.
X80700 Genomic DNA. Translation: CAA56717.1.
D28769 Genomic DNA. Translation: BAA05957.1.
U89336 Genomic DNA. Translation: AAB47490.1.
AL662884 Genomic DNA. Translation: CAI18358.1.
AL662830 Genomic DNA. Translation: CAI17539.1.
AL845464 Genomic DNA. Translation: CAI41814.1.
BX284686 Genomic DNA. Translation: CAM26228.1.
BX927239 Genomic DNA. Translation: CAQ06600.1.
CR933878 Genomic DNA. Translation: CAQ09629.1.
CR812478 Genomic DNA. Translation: CAQ10704.1.
CH471081 Genomic DNA. Translation: EAX03613.1.
BC082261 mRNA. Translation: AAH82261.1.
CCDSCCDS4748.1.
PIRA56002.
RefSeqNP_002577.2. NM_002586.4.
UniGeneHs.509545.
Hs.718462.

3D structure databases

ProteinModelPortalP40425.
SMRP40425. Positions 244-316.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111122. 3 interactions.
IntActP40425. 20 interactions.
MINTMINT-1032522.
STRING9606.ENSP00000397177.

PTM databases

PhosphoSiteP40425.

Polymorphism databases

DMDM1352729.

Proteomic databases

MaxQBP40425.
PaxDbP40425.
PRIDEP40425.

Protocols and materials databases

DNASU5089.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000375050; ENSP00000364190; ENSG00000204304.
ENST00000383270; ENSP00000372757; ENSG00000206315.
ENST00000420432; ENSP00000410710; ENSG00000236353.
ENST00000431537; ENSP00000390009; ENSG00000232005.
ENST00000433552; ENSP00000391745; ENSG00000224952.
ENST00000453487; ENSP00000403948; ENSG00000225987.
ENST00000453961; ENSP00000397177; ENSG00000237344.
GeneID5089.
KEGGhsa:5089.
UCSCuc003oav.1. human.

Organism-specific databases

CTD5089.
GeneCardsGC06M032153.
GC06Mi32163.
GC06Mj32100.
GC06Ml32191.
GC06Mm32228.
GC06Mn32110.
GC06Mo32159.
HGNCHGNC:8633. PBX2.
HPAHPA054000.
HPA061478.
MIM176311. gene.
neXtProtNX_P40425.
PharmGKBPA32971.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG248144.
HOVERGENHBG000122.
InParanoidP40425.
KOK15609.
OMAGQIYSPR.
OrthoDBEOG75XGM1.
PhylomeDBP40425.
TreeFamTF314340.

Gene expression databases

BgeeP40425.
CleanExHS_PBX2.
GenevestigatorP40425.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR005542. PBX.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
PF03792. PBC. 1 hit.
[Graphical view]
SMARTSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPBX2. human.
GeneWikiPBX2.
GenomeRNAi5089.
NextBio19624.
PROP40425.
SOURCESearch...

Entry information

Entry namePBX2_HUMAN
AccessionPrimary (citable) accession number: P40425
Secondary accession number(s): A2BFJ2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1996
Last modified: July 9, 2014
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM