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P40423

- SQH_DROME

UniProt

P40423 - SQH_DROME

Protein

Myosin regulatory light chain sqh

Gene

sqh

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Required for cytokinesis, could regulate contractile ring function.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi44 – 5512PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. myosin heavy chain binding Source: FlyBase

    GO - Biological processi

    1. actin filament-based movement Source: FlyBase
    2. border follicle cell migration Source: FlyBase
    3. cell elongation involved in imaginal disc-derived wing morphogenesis Source: FlyBase
    4. cellularization Source: FlyBase
    5. cytoskeleton-dependent cytoplasmic transport, nurse cell to oocyte Source: FlyBase
    6. establishment of planar polarity Source: FlyBase
    7. formation of a compartment boundary Source: FlyBase
    8. imaginal disc-derived wing hair organization Source: FlyBase
    9. mitotic cytokinesis Source: FlyBase
    10. neurogenesis Source: FlyBase
    11. nuclear axial expansion Source: FlyBase
    12. ovarian follicle cell development Source: FlyBase
    13. ovarian nurse cell to oocyte transport Source: FlyBase
    14. regulation of actin cytoskeleton organization Source: FlyBase
    15. regulation of tube length, open tracheal system Source: FlyBase
    16. spiracle morphogenesis, open tracheal system Source: FlyBase
    17. wound healing Source: FlyBase

    Keywords - Molecular functioni

    Motor protein, Myosin

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_211028. Smooth Muscle Contraction.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myosin regulatory light chain sqh
    Alternative name(s):
    Myosin regulatory light chain, nonmuscle
    Short name:
    MRLC-C
    Protein spaghetti-squash
    Gene namesi
    Name:sqh
    ORF Names:CG3595
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome X

    Organism-specific databases

    FlyBaseiFBgn0003514. sqh.

    Subcellular locationi

    GO - Cellular componenti

    1. adherens junction Source: FlyBase
    2. apical plasma membrane Source: FlyBase
    3. cell cortex Source: FlyBase
    4. cleavage furrow Source: FlyBase
    5. cytoplasm Source: FlyBase
    6. female germline ring canal inner rim Source: FlyBase
    7. midbody Source: FlyBase
    8. myosin II complex Source: FlyBase
    9. plasma membrane Source: FlyBase
    10. spindle midzone Source: FlyBase

    Pathology & Biotechi

    Disruption phenotypei

    Mitotic defects including failure in cytokinesis.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 174174Myosin regulatory light chain sqhPRO_0000198747Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei21 – 211Phosphothreonine2 Publications
    Modified residuei22 – 221Phosphoserine2 Publications

    Post-translational modificationi

    Phosphorylation plays a central role in myosin regulation.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP40423.
    PRIDEiP40423.

    Expressioni

    Gene expression databases

    BgeeiP40423.

    Interactioni

    Subunit structurei

    Myosin is a hexamer of 2 heavy chains and 4 light chains.

    Protein-protein interaction databases

    BioGridi58046. 13 interactions.
    IntActiP40423. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP40423.
    SMRiP40423. Positions 28-169.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini31 – 6636EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini100 – 13536EF-hand 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 2 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG5126.
    GeneTreeiENSGT00730000110490.
    InParanoidiP40423.
    KOiK12757.
    OMAiNEEHLRE.
    OrthoDBiEOG7992RX.
    PhylomeDBiP40423.

    Family and domain databases

    Gene3Di1.10.238.10. 2 hits.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view]
    PfamiPF13499. EF-hand_7. 1 hit.
    [Graphical view]
    SMARTiSM00054. EFh. 2 hits.
    [Graphical view]
    PROSITEiPS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40423-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSRKTAGRR ATTKKRAQRA TSNVFAMFDQ AQIAEFKEAF NMIDQNRDGF    50
    VEKEDLHDML ASLGKNPTDD YLDGMMNEAP GPINFTMFLT LFGERLQGTD 100
    PEDVIKNAFG CFDEENMGVL PEDRLRELLT TMGDRFTDED VDEMYREAPI 150
    KNGLFDYLEF TRILKHGAKD KDEQ 174
    Length:174
    Mass (Da):19,954
    Last modified:February 1, 1995 - v1
    Checksum:i2D95C9F0460B8766
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M67494 Genomic DNA. Translation: AAC13367.1.
    AE014298 Genomic DNA. Translation: AAF46132.1.
    AY122159 mRNA. Translation: AAM52671.1.
    PIRiA39932.
    RefSeqiNP_511057.1. NM_078502.2.
    UniGeneiDm.3.

    Genome annotation databases

    EnsemblMetazoaiFBtr0070877; FBpp0070842; FBgn0003514.
    GeneIDi31554.
    KEGGidme:Dmel_CG3595.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M67494 Genomic DNA. Translation: AAC13367.1 .
    AE014298 Genomic DNA. Translation: AAF46132.1 .
    AY122159 mRNA. Translation: AAM52671.1 .
    PIRi A39932.
    RefSeqi NP_511057.1. NM_078502.2.
    UniGenei Dm.3.

    3D structure databases

    ProteinModelPortali P40423.
    SMRi P40423. Positions 28-169.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 58046. 13 interactions.
    IntActi P40423. 1 interaction.

    Proteomic databases

    PaxDbi P40423.
    PRIDEi P40423.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0070877 ; FBpp0070842 ; FBgn0003514 .
    GeneIDi 31554.
    KEGGi dme:Dmel_CG3595.

    Organism-specific databases

    CTDi 31554.
    FlyBasei FBgn0003514. sqh.

    Phylogenomic databases

    eggNOGi COG5126.
    GeneTreei ENSGT00730000110490.
    InParanoidi P40423.
    KOi K12757.
    OMAi NEEHLRE.
    OrthoDBi EOG7992RX.
    PhylomeDBi P40423.

    Enzyme and pathway databases

    Reactomei REACT_211028. Smooth Muscle Contraction.

    Miscellaneous databases

    GenomeRNAii 31554.
    NextBioi 774178.

    Gene expression databases

    Bgeei P40423.

    Family and domain databases

    Gene3Di 1.10.238.10. 2 hits.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view ]
    Pfami PF13499. EF-hand_7. 1 hit.
    [Graphical view ]
    SMARTi SM00054. EFh. 2 hits.
    [Graphical view ]
    PROSITEi PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The regulatory light chain of nonmuscle myosin is encoded by spaghetti-squash, a gene required for cytokinesis in Drosophila."
      Karess R.E., Chang X.J., Edwards K.A., Kulkarni S., Aguilera I., Kiehart D.P.
      Cell 65:1177-1189(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    3. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Embryo.
    5. "An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells."
      Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A.
      Mol. Biosyst. 3:275-286(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-21 AND SER-22, IDENTIFICATION BY MASS SPECTROMETRY.
    6. "Phosphoproteome analysis of Drosophila melanogaster embryos."
      Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
      J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-21 AND SER-22, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Embryo.

    Entry informationi

    Entry nameiSQH_DROME
    AccessioniPrimary (citable) accession number: P40423
    Secondary accession number(s): Q540V2, Q9W428
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Miscellaneous

    This chain binds calcium.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3