P40423 (SQH_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myosin regulatory light chain sqh

Alternative name(s):
Myosin regulatory light chain, nonmuscle
Short name=MRLC-C
Protein spaghetti-squash

Gene names

Name:	sqh
ORF Names:	CG3595

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	174 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for cytokinesis, could regulate contractile ring function. Ref.1
Subunit structure	Myosin is a hexamer of 2 heavy chains and 4 light chains.
Post-translational modification	Phosphorylation plays a central role in myosin regulation By similarity.
Disruption phenotype	Mitotic defects including failure in cytokinesis. Ref.1
Miscellaneous	This chain binds calcium By similarity.
Sequence similarities	Contains 2 EF-hand domains.

Ontologies

Keywords
Domain	Repeat
Ligand	Calcium Metal-binding
Molecular function	Motor protein Myosin
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin filament-based movement Inferred by curator PubMed 16917818. Source: FlyBase border follicle cell migration Inferred from mutant phenotype PubMed 12198500. Source: FlyBase cell elongation involved in imaginal disc-derived wing morphogenesis Inferred from mutant phenotype PubMed 19366729. Source: FlyBase cellularization Non-traceable author statement PubMed 11258476. Source: FlyBase cytokinesis Inferred from direct assay PubMed 17205135. Source: FlyBase establishment of planar polarity Non-traceable author statement PubMed 12414186. Source: FlyBase formation of a compartment boundary Inferred from direct assay PubMed 19966783. Source: FlyBase imaginal disc-derived wing hair organization Inferred from genetic interaction PubMed 11301004. Source: FlyBase neurogenesis Inferred from mutant phenotype PubMed 21549331. Source: FlyBase nuclear axial expansion Inferred from mutant phenotype PubMed 14657248. Source: FlyBase ovarian nurse cell to oocyte transport Traceable author statement PubMed 11139280. Source: FlyBase regulation of actin cytoskeleton organization Inferred from mutant phenotype PubMed 18207738. Source: FlyBase regulation of tube length, open tracheal system Inferred from mutant phenotype PubMed 19956736. Source: FlyBase wound healing Inferred from mutant phenotype PubMed 21518790. Source: FlyBase
Cellular_component	adherens junction Inferred from direct assay PubMed 20920606. Source: FlyBase apical plasma membrane Inferred from direct assay PubMed 20920606. Source: FlyBase cell cortex Inferred from direct assay PubMed 12105185. Source: FlyBase cleavage furrow Inferred from direct assay PubMed 16174742PubMed 20920606. Source: FlyBase midbody Inferred from direct assay PubMed 16174742. Source: FlyBase myosin II complex Inferred from physical interaction PubMed 16917818. Source: FlyBase spindle midzone Inferred from direct assay PubMed 16174742. Source: FlyBase
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 174	174	Myosin regulatory light chain sqh		PRO_0000198747
Regions
	Domain	31 – 66	36	EF-hand 1
	Domain	100 – 135	36	EF-hand 2
	Calcium binding	44 – 55	12	Potential
Amino acid modifications
	Modified residue	21	1	Phosphothreonine Ref.5 Ref.6
	Modified residue	22	1	Phosphoserine Ref.5 Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P40423 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 2D95C9F0460B8766	FASTA	174	19,954
	10 20 30 40 50 60 MSSRKTAGRR ATTKKRAQRA TSNVFAMFDQ AQIAEFKEAF NMIDQNRDGF VEKEDLHDML 70 80 90 100 110 120 ASLGKNPTDD YLDGMMNEAP GPINFTMFLT LFGERLQGTD PEDVIKNAFG CFDEENMGVL 130 140 150 160 170 PEDRLRELLT TMGDRFTDED VDEMYREAPI KNGLFDYLEF TRILKHGAKD KDEQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The regulatory light chain of nonmuscle myosin is encoded by spaghetti-squash, a gene required for cytokinesis in Drosophila." Karess R.E., Chang X.J., Edwards K.A., Kulkarni S., Aguilera I., Kiehart D.P. Cell 65:1177-1189(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells." Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A. Mol. Biosyst. 3:275-286(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-21 AND SER-22, MASS SPECTROMETRY.
[6]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-21 AND SER-22, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M67494 Genomic DNA. Translation: AAC13367.1. AE014298 Genomic DNA. Translation: AAF46132.1. AY122159 mRNA. Translation: AAM52671.1.
PIR	A39932.
RefSeq	NP_511057.1. NM_078502.2.
UniGene	Dm.3.
3D structure databases
ProteinModelPortal	P40423.
SMR	P40423. Positions 28-169.
ModBase	Search...
Protein-protein interaction databases
IntAct	P40423. 1 interaction.
Proteomic databases
PaxDb	P40423.
PRIDE	P40423.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0070877; FBpp0070842; FBgn0003514.
GeneID	31554.
KEGG	dme:Dmel_CG3595.
Organism-specific databases
CTD	31554.
FlyBase	FBgn0003514. sqh.
Phylogenomic databases
eggNOG	COG5126.
GeneTree	ENSGT00590000082808.
InParanoid	P40423.
KO	K12759.
OMA	RKTVNRR.
OrthoDB	EOG4W0VWC.
PhylomeDB	P40423.
Gene expression databases
Bgee	P40423.
GermOnline	CG3595. Drosophila melanogaster.
Family and domain databases
Gene3D	1.10.238.10. 2 hits.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. [Graphical view]
Pfam	PF13499. EF_hand_5. 1 hit. [Graphical view]
SMART	SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 1 hit. PS50222. EF_HAND_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	31554.
NextBio	774178.

Entry information

Entry name

SQH_DROME

Accession

Primary (citable) accession number: P40423
Secondary accession number(s): Q540V2, Q9W428

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project