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Protein

DNA-directed RNA polymerases I, II, and III subunit RPABC4

Gene

RPC10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and a small RNAs, such as 5S rRNA and tRNAs, respectively. RNA polymerases are composed of mobile elements that move relative to each other. In Pol II, the core element with the central large cleft comprises RPB3, RBP10, RPB11, RPB12 and regions of RPB1 and RPB2 forming the active center.3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi31 – 311Zinc
Metal bindingi34 – 341Zinc
Metal bindingi48 – 481Zinc
Metal bindingi51 – 511Zinc

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri31 – 5121C4-typeAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: InterPro
  • RNA polymerase I activity Source: UniProtKB
  • zinc ion binding Source: SGD

GO - Biological processi

  • ribosome biogenesis Source: UniProtKB-KW
  • termination of RNA polymerase III transcription Source: Reactome
  • transcription, RNA-templated Source: GOC
  • transcription elongation from RNA polymerase III promoter Source: Reactome
  • transcription from RNA polymerase III promoter Source: SGD
  • transcription from RNA polymerase II promoter Source: SGD
  • transcription from RNA polymerase I promoter Source: UniProtKB
  • tRNA transcription from RNA polymerase III promoter Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ribosome biogenesis, Transcription

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-31179-MONOMER.
ReactomeiR-SCE-73780. RNA Polymerase III Chain Elongation.
R-SCE-73980. RNA Polymerase III Transcription Termination.
R-SCE-749476. RNA Polymerase III Abortive And Retractive Initiation.
R-SCE-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-SCE-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-SCE-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerases I, II, and III subunit RPABC4
Short name:
RNA polymerases I, II, and III subunit ABC4
Alternative name(s):
ABC10-alpha
Gene namesi
Name:RPC10
Synonyms:RPB12
Ordered Locus Names:YHR143W-A
ORF Names:YHR143BW
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHR143W-A.
SGDiS000001185. RPC10.

Subcellular locationi

GO - Cellular componenti

  • DNA-directed RNA polymerase I complex Source: UniProtKB
  • DNA-directed RNA polymerase II, core complex Source: SGD
  • DNA-directed RNA polymerase III complex Source: SGD
  • nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7070DNA-directed RNA polymerases I, II, and III subunit RPABC4PRO_0000159753Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

MaxQBiP40422.

PTM databases

iPTMnetiP40422.

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I), RNA polymerase II (Pol II) and RNA polymerase III (Pol III) complexes. Component of the RNA polymerase I (Pol I) complex consisting of 14 subunits: RPA135, RPA190, RPC40, RPA14, RPB5, RPO26, RPA43, RPB8, RPA12, RPB10, RPC19, RPC10, RPA49 and RPA34. The complex is composed of a horseshoe-shaped core containing ten subunits (RPA135, RPA190, RPB5, RPO26, RPB8, RPB10, RPC10, RPA12, RPC19 and RPC40) where RPA135 and RPA190 form the DNA-binding cleft. Outside of the core, RPA14 and RPA43 form the stalk that mediates interactions with transcription initiation factors and newly synthesized RNA. Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits: RPO21, RPB2, RPB3, RPB4, RPB5, RPO26, RPB7, RPB8, RPB9, RPB10 and RPC10. Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits. Interacts, via its C-terminus, with TFIIIC subunit TFC4.7 Publications

Protein-protein interaction databases

BioGridi36577. 62 interactions.
DIPiDIP-936N.
IntActiP40422. 7 interactions.
MINTiMINT-559375.

Structurei

Secondary structure

1
70
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi28 – 314Combined sources
Beta strandi32 – 343Combined sources
Beta strandi36 – 383Combined sources
Beta strandi41 – 444Combined sources
Beta strandi46 – 483Combined sources
Beta strandi49 – 513Combined sources
Beta strandi56 – 583Combined sources
Beta strandi65 – 673Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1I3QX-ray3.10L1-70[»]
1I50X-ray2.80L1-70[»]
1I6HX-ray3.30L1-70[»]
1K83X-ray2.80L1-70[»]
1NIKX-ray4.10L1-70[»]
1NT9X-ray4.20L1-70[»]
1PQVX-ray3.80L1-70[»]
1R5UX-ray4.50L1-70[»]
1R9SX-ray4.25L1-70[»]
1R9TX-ray3.50L1-70[»]
1SFOX-ray3.61L1-70[»]
1TWAX-ray3.20L1-70[»]
1TWCX-ray3.00L1-70[»]
1TWFX-ray2.30L1-70[»]
1TWGX-ray3.30L1-70[»]
1TWHX-ray3.40L1-70[»]
1WCMX-ray3.80L1-70[»]
1Y1VX-ray3.80L1-70[»]
1Y1WX-ray4.00L1-70[»]
1Y1YX-ray4.00L1-70[»]
1Y77X-ray4.50L1-70[»]
2B63X-ray3.80L1-70[»]
2B8KX-ray4.15L1-70[»]
2E2HX-ray3.95L1-70[»]
2E2IX-ray3.41L1-70[»]
2E2JX-ray3.50L1-70[»]
2JA5X-ray3.80L1-70[»]
2JA6X-ray4.00L1-70[»]
2JA7X-ray3.80L/X1-70[»]
2JA8X-ray3.80L1-70[»]
2NVQX-ray2.90L1-70[»]
2NVTX-ray3.36L1-70[»]
2NVXX-ray3.60L1-70[»]
2NVYX-ray3.40L1-70[»]
2NVZX-ray4.30L1-70[»]
2R7ZX-ray3.80L1-70[»]
2R92X-ray3.80L1-70[»]
2R93X-ray4.00L1-70[»]
2VUMX-ray3.40L1-70[»]
2YU9X-ray3.40L1-70[»]
3CQZX-ray2.80L1-70[»]
3FKIX-ray3.88L1-70[»]
3GTGX-ray3.78L1-70[»]
3GTJX-ray3.42L1-70[»]
3GTKX-ray3.80L1-70[»]
3GTLX-ray3.38L1-70[»]
3GTMX-ray3.80L1-70[»]
3GTOX-ray4.00L1-70[»]
3GTPX-ray3.90L1-70[»]
3GTQX-ray3.80L1-70[»]
3H3VX-ray4.00M1-70[»]
3HOUX-ray3.20L/X1-70[»]
3HOVX-ray3.50L1-70[»]
3HOWX-ray3.60L1-70[»]
3HOXX-ray3.65L1-70[»]
3HOYX-ray3.40L1-70[»]
3HOZX-ray3.65L1-70[»]
3I4MX-ray3.70L1-70[»]
3I4NX-ray3.90L1-70[»]
3J1Nelectron microscopy16.00L1-70[»]
3K1FX-ray4.30L1-70[»]
3K7AX-ray3.80L1-70[»]
3M3YX-ray3.18L1-70[»]
3M4OX-ray3.57L1-70[»]
3PO2X-ray3.30L1-70[»]
3PO3X-ray3.30L1-70[»]
3QT1X-ray4.30L1-70[»]
3RZDX-ray3.30L1-70[»]
3RZOX-ray3.00L1-70[»]
3S14X-ray2.85L1-70[»]
3S15X-ray3.30L1-70[»]
3S16X-ray3.24L1-70[»]
3S17X-ray3.20L1-70[»]
3S1MX-ray3.13L1-70[»]
3S1NX-ray3.10L1-70[»]
3S1QX-ray3.30L1-70[»]
3S1RX-ray3.20L1-70[»]
3S2DX-ray3.20L1-70[»]
3S2HX-ray3.30L1-70[»]
4A3BX-ray3.50L1-70[»]
4A3CX-ray3.50L1-70[»]
4A3DX-ray3.40L1-70[»]
4A3EX-ray3.40L1-70[»]
4A3FX-ray3.50L1-70[»]
4A3GX-ray3.50L1-70[»]
4A3IX-ray3.80L1-70[»]
4A3JX-ray3.70L1-70[»]
4A3KX-ray3.50L1-70[»]
4A3LX-ray3.50L1-70[»]
4A3MX-ray3.90L1-70[»]
4A93X-ray3.40L1-70[»]
4BBRX-ray3.40L1-70[»]
4BBSX-ray3.60L1-70[»]
4BXXX-ray3.28L1-70[»]
4BXZX-ray4.80L1-70[»]
4BY1X-ray3.60L1-70[»]
4BY7X-ray3.15L1-70[»]
4C2MX-ray2.801/L1-70[»]
4C3HX-ray3.27L1-70[»]
4C3IX-ray3.0L1-70[»]
4C3JX-ray3.35L1-70[»]
4V1Melectron microscopy6.60L1-70[»]
4V1Nelectron microscopy7.80L1-70[»]
4V1Oelectron microscopy9.70L1-70[»]
4X67X-ray4.10L1-70[»]
4X6AX-ray3.96L1-70[»]
4Y52X-ray3.50L1-70[»]
4Y7NX-ray3.30L1-70[»]
4YM7X-ray5.50AL/BL/CL/DL/EL/FL1-70[»]
5C3EX-ray3.70L1-70[»]
5C44X-ray3.95L1-70[»]
5C4AX-ray4.20L1-70[»]
5C4JX-ray4.00L1-70[»]
5C4XX-ray4.00L1-70[»]
5FJ8electron microscopy3.90L1-70[»]
5FJ9electron microscopy4.60L1-70[»]
5FJAelectron microscopy4.65L1-70[»]
5FMFelectron microscopy6.00L25-70[»]
5FYWelectron microscopy4.35L1-70[»]
5FZ5electron microscopy8.80L1-70[»]
5IP7X-ray3.52L25-70[»]
5IP9X-ray3.90L25-70[»]
ProteinModelPortaliP40422.
SMRiP40422. Positions 25-70.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40422.

Family & Domainsi

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri31 – 5121C4-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000191755.
InParanoidiP40422.
KOiK03009.
OMAiGECNARV.
OrthoDBiEOG092C5YM4.

Family and domain databases

Gene3Di2.20.28.30. 1 hit.
InterProiIPR006591. RNAP_P/RPABC4.
IPR029040. RPABC4/Spt4.
[Graphical view]
PfamiPF03604. DNA_RNApol_7kD. 1 hit.
[Graphical view]
SMARTiSM00659. RPOLCX. 1 hit.
[Graphical view]
SUPFAMiSSF63393. SSF63393. 1 hit.

Sequencei

Sequence statusi: Complete.

P40422-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSREGFQIPT NLDAAAAGTS QARTATLKYI CAECSSKLSL SRTDAVRCKD
60 70
CGHRILLKAR TKRLVQFEAR
Length:70
Mass (Da):7,716
Last modified:February 1, 1995 - v1
Checksum:i066A3D982EC7361E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23378 Genomic DNA. Translation: AAA64417.1.
U10397 Genomic DNA. Translation: AAB68994.1.
AY558549 Genomic DNA. Translation: AAS56875.1.
BK006934 Genomic DNA. Translation: DAA06837.1.
PIRiS58932.
RefSeqiNP_012013.1. NM_001179273.1.

Genome annotation databases

EnsemblFungiiYHR143W-A; YHR143W-A; YHR143W-A.
GeneIDi856547.
KEGGisce:YHR143W-A.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U23378 Genomic DNA. Translation: AAA64417.1.
U10397 Genomic DNA. Translation: AAB68994.1.
AY558549 Genomic DNA. Translation: AAS56875.1.
BK006934 Genomic DNA. Translation: DAA06837.1.
PIRiS58932.
RefSeqiNP_012013.1. NM_001179273.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1I3QX-ray3.10L1-70[»]
1I50X-ray2.80L1-70[»]
1I6HX-ray3.30L1-70[»]
1K83X-ray2.80L1-70[»]
1NIKX-ray4.10L1-70[»]
1NT9X-ray4.20L1-70[»]
1PQVX-ray3.80L1-70[»]
1R5UX-ray4.50L1-70[»]
1R9SX-ray4.25L1-70[»]
1R9TX-ray3.50L1-70[»]
1SFOX-ray3.61L1-70[»]
1TWAX-ray3.20L1-70[»]
1TWCX-ray3.00L1-70[»]
1TWFX-ray2.30L1-70[»]
1TWGX-ray3.30L1-70[»]
1TWHX-ray3.40L1-70[»]
1WCMX-ray3.80L1-70[»]
1Y1VX-ray3.80L1-70[»]
1Y1WX-ray4.00L1-70[»]
1Y1YX-ray4.00L1-70[»]
1Y77X-ray4.50L1-70[»]
2B63X-ray3.80L1-70[»]
2B8KX-ray4.15L1-70[»]
2E2HX-ray3.95L1-70[»]
2E2IX-ray3.41L1-70[»]
2E2JX-ray3.50L1-70[»]
2JA5X-ray3.80L1-70[»]
2JA6X-ray4.00L1-70[»]
2JA7X-ray3.80L/X1-70[»]
2JA8X-ray3.80L1-70[»]
2NVQX-ray2.90L1-70[»]
2NVTX-ray3.36L1-70[»]
2NVXX-ray3.60L1-70[»]
2NVYX-ray3.40L1-70[»]
2NVZX-ray4.30L1-70[»]
2R7ZX-ray3.80L1-70[»]
2R92X-ray3.80L1-70[»]
2R93X-ray4.00L1-70[»]
2VUMX-ray3.40L1-70[»]
2YU9X-ray3.40L1-70[»]
3CQZX-ray2.80L1-70[»]
3FKIX-ray3.88L1-70[»]
3GTGX-ray3.78L1-70[»]
3GTJX-ray3.42L1-70[»]
3GTKX-ray3.80L1-70[»]
3GTLX-ray3.38L1-70[»]
3GTMX-ray3.80L1-70[»]
3GTOX-ray4.00L1-70[»]
3GTPX-ray3.90L1-70[»]
3GTQX-ray3.80L1-70[»]
3H3VX-ray4.00M1-70[»]
3HOUX-ray3.20L/X1-70[»]
3HOVX-ray3.50L1-70[»]
3HOWX-ray3.60L1-70[»]
3HOXX-ray3.65L1-70[»]
3HOYX-ray3.40L1-70[»]
3HOZX-ray3.65L1-70[»]
3I4MX-ray3.70L1-70[»]
3I4NX-ray3.90L1-70[»]
3J1Nelectron microscopy16.00L1-70[»]
3K1FX-ray4.30L1-70[»]
3K7AX-ray3.80L1-70[»]
3M3YX-ray3.18L1-70[»]
3M4OX-ray3.57L1-70[»]
3PO2X-ray3.30L1-70[»]
3PO3X-ray3.30L1-70[»]
3QT1X-ray4.30L1-70[»]
3RZDX-ray3.30L1-70[»]
3RZOX-ray3.00L1-70[»]
3S14X-ray2.85L1-70[»]
3S15X-ray3.30L1-70[»]
3S16X-ray3.24L1-70[»]
3S17X-ray3.20L1-70[»]
3S1MX-ray3.13L1-70[»]
3S1NX-ray3.10L1-70[»]
3S1QX-ray3.30L1-70[»]
3S1RX-ray3.20L1-70[»]
3S2DX-ray3.20L1-70[»]
3S2HX-ray3.30L1-70[»]
4A3BX-ray3.50L1-70[»]
4A3CX-ray3.50L1-70[»]
4A3DX-ray3.40L1-70[»]
4A3EX-ray3.40L1-70[»]
4A3FX-ray3.50L1-70[»]
4A3GX-ray3.50L1-70[»]
4A3IX-ray3.80L1-70[»]
4A3JX-ray3.70L1-70[»]
4A3KX-ray3.50L1-70[»]
4A3LX-ray3.50L1-70[»]
4A3MX-ray3.90L1-70[»]
4A93X-ray3.40L1-70[»]
4BBRX-ray3.40L1-70[»]
4BBSX-ray3.60L1-70[»]
4BXXX-ray3.28L1-70[»]
4BXZX-ray4.80L1-70[»]
4BY1X-ray3.60L1-70[»]
4BY7X-ray3.15L1-70[»]
4C2MX-ray2.801/L1-70[»]
4C3HX-ray3.27L1-70[»]
4C3IX-ray3.0L1-70[»]
4C3JX-ray3.35L1-70[»]
4V1Melectron microscopy6.60L1-70[»]
4V1Nelectron microscopy7.80L1-70[»]
4V1Oelectron microscopy9.70L1-70[»]
4X67X-ray4.10L1-70[»]
4X6AX-ray3.96L1-70[»]
4Y52X-ray3.50L1-70[»]
4Y7NX-ray3.30L1-70[»]
4YM7X-ray5.50AL/BL/CL/DL/EL/FL1-70[»]
5C3EX-ray3.70L1-70[»]
5C44X-ray3.95L1-70[»]
5C4AX-ray4.20L1-70[»]
5C4JX-ray4.00L1-70[»]
5C4XX-ray4.00L1-70[»]
5FJ8electron microscopy3.90L1-70[»]
5FJ9electron microscopy4.60L1-70[»]
5FJAelectron microscopy4.65L1-70[»]
5FMFelectron microscopy6.00L25-70[»]
5FYWelectron microscopy4.35L1-70[»]
5FZ5electron microscopy8.80L1-70[»]
5IP7X-ray3.52L25-70[»]
5IP9X-ray3.90L25-70[»]
ProteinModelPortaliP40422.
SMRiP40422. Positions 25-70.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36577. 62 interactions.
DIPiDIP-936N.
IntActiP40422. 7 interactions.
MINTiMINT-559375.

PTM databases

iPTMnetiP40422.

Proteomic databases

MaxQBiP40422.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHR143W-A; YHR143W-A; YHR143W-A.
GeneIDi856547.
KEGGisce:YHR143W-A.

Organism-specific databases

EuPathDBiFungiDB:YHR143W-A.
SGDiS000001185. RPC10.

Phylogenomic databases

HOGENOMiHOG000191755.
InParanoidiP40422.
KOiK03009.
OMAiGECNARV.
OrthoDBiEOG092C5YM4.

Enzyme and pathway databases

BioCyciYEAST:G3O-31179-MONOMER.
ReactomeiR-SCE-73780. RNA Polymerase III Chain Elongation.
R-SCE-73980. RNA Polymerase III Transcription Termination.
R-SCE-749476. RNA Polymerase III Abortive And Retractive Initiation.
R-SCE-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-SCE-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-SCE-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Miscellaneous databases

EvolutionaryTraceiP40422.
PROiP40422.

Family and domain databases

Gene3Di2.20.28.30. 1 hit.
InterProiIPR006591. RNAP_P/RPABC4.
IPR029040. RPABC4/Spt4.
[Graphical view]
PfamiPF03604. DNA_RNApol_7kD. 1 hit.
[Graphical view]
SMARTiSM00659. RPOLCX. 1 hit.
[Graphical view]
SUPFAMiSSF63393. SSF63393. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRPAB4_YEAST
AccessioniPrimary (citable) accession number: P40422
Secondary accession number(s): D3DL93
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 7, 2016
This is version 161 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 907 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.