P40421 (RDGC_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase rdgC
EC=3.1.3.16

Alternative name(s):
Retinal degeneration C protein

Gene names

Name:	rdgC
ORF Names:	CG6571

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	661 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Phosphatase required to prevent light-induced retinal degeneration.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Enzyme regulation	Regulated by calcium.
Tissue specificity	Expressed in the visual systems of the fly, as well as in the mushroom bodies of the central brain.
Sequence similarities	Belongs to the PPP phosphatase family. Contains 3 EF-hand domains. Contains 1 IQ domain.

Ontologies

Keywords
Biological process	Sensory transduction Vision
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	Calcium Iron Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	calcium-mediated signaling Traceable author statement PubMed 10611962. Source: FlyBase deactivation of rhodopsin mediated signaling Inferred from mutant phenotype PubMed 11754840. Source: FlyBase detection of stimulus involved in sensory perception Inferred from electronic annotation. Source: InterPro phototransduction Inferred from mutant phenotype PubMed 2361011PubMed 8446607. Source: FlyBase protein dephosphorylation Inferred from mutant phenotype PubMed 8446607. Source: FlyBase thermotaxis Inferred from direct assay PubMed 18660806. Source: FlyBase visual perception Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro calcium-dependent protein serine/threonine phosphatase activity Inferred from sequence or structural similarity Ref.1. Source: FlyBase calmodulin binding Inferred from direct assay PubMed 11754840. Source: FlyBase iron ion binding Inferred from electronic annotation. Source: InterPro manganese ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform B (identifier: P40421-1)

Also known as: C; D;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform A (identifier: P40421-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-58: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 661	661	Serine/threonine-protein phosphatase rdgC		PRO_0000058903
Regions
	Domain	7 – 32	26	IQ
	Domain	441 – 476	36	EF-hand 1
	Domain	526 – 561	36	EF-hand 2
	Domain	566 – 601	36	EF-hand 3
	Calcium binding	539 – 550	12	1 Potential
	Calcium binding	579 – 590	12	2 Potential
	Region	105 – 413	309	Catalytic
Sites
	Active site	220	1	Proton donor By similarity
	Metal binding	158	1	Iron By similarity
	Metal binding	160	1	Iron By similarity
	Metal binding	187	1	Iron By similarity
	Metal binding	187	1	Manganese By similarity
	Metal binding	219	1	Manganese By similarity
	Metal binding	271	1	Manganese By similarity
	Metal binding	360	1	Manganese By similarity
Natural variations
	Alternative sequence	1 – 58	58	Missing in isoform A.		VSP_009324
Experimental info
	Sequence conflict	522	1	D → G in AAV36844. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform B (C) (D) [UniParc]. Last modified February 1, 1995. Version 1. Checksum: A3DC42933E4CCA33	FASTA	661	75,511
	10 20 30 40 50 60 MDENAIRAAI FIQKWYRRHQ ARREMQRRCN WQIFQNLEYA SEQDQAELYK FFNDLIKHMP 70 80 90 100 110 120 QAAGRKNQYQ GSAHVSVLDD KDDLVEEFGD IVNAKIELPI RKNHIDLLID VFRKKRGNRL 130 140 150 160 170 180 HPKYVALILR EAAKSLKQLP NISPVSTAVS QQVTVCGDLH GKLDDLLVVL HKNGLPSSSN 190 200 210 220 230 240 PYVFNGDFVD RGKRGLEVLL LLLSLYLAFP NAVFLNRGNH EDSVMNARYG FIREVESKYP 250 260 270 280 290 300 RNHKRILAFI DEVYRWLPLG SVLNSRVLIV HGGFSDSTSL DLIKSIDRGK YVSILRPPLT 310 320 330 340 350 360 DGEPLDKTEW QQIFDIMWSD PQATMGCVPN TLRGAGVWFG PDVTDNFLQR HRLSYVIRSH 370 380 390 400 410 420 ECKPNGHEFM HDNKIITIFS ASNYYAIGSN KGAYIRLNNQ LMPHFVQYIS AASQTKRLSF 430 440 450 460 470 480 KQRMGIVESS ALKELAVRMR DHRDELEDEF RKYDPKDSGY ISISHWCKVM ENVTKLGLPW 490 500 510 520 530 540 RLLRDKLAPG TDSQKVNYNR TLDLLDTDVI LEAEADGMSV MDALYANKAS LVAIFNIIDA 550 560 570 580 590 600 DNSGEITLDE FETAIDLLVA HMPGAYSKAE MLEKCRMMDL NGDGKVDLNE FLEAFRLSDL 610 620 630 640 650 660 HRKEQQDENI RRRSTGRPSV AKTATDPVTL LADKISKNTL VVEHDIDPTD CESKVIDPKK S
	Isoform A [UniParc]. Checksum: A2F9A7FB905DEB03	FASTA	603	68,180
	10 20 30 40 50 60 MPQAAGRKNQ YQGSAHVSVL DDKDDLVEEF GDIVNAKIEL PIRKNHIDLL IDVFRKKRGN 70 80 90 100 110 120 RLHPKYVALI LREAAKSLKQ LPNISPVSTA VSQQVTVCGD LHGKLDDLLV VLHKNGLPSS 130 140 150 160 170 180 SNPYVFNGDF VDRGKRGLEV LLLLLSLYLA FPNAVFLNRG NHEDSVMNAR YGFIREVESK 190 200 210 220 230 240 YPRNHKRILA FIDEVYRWLP LGSVLNSRVL IVHGGFSDST SLDLIKSIDR GKYVSILRPP 250 260 270 280 290 300 LTDGEPLDKT EWQQIFDIMW SDPQATMGCV PNTLRGAGVW FGPDVTDNFL QRHRLSYVIR 310 320 330 340 350 360 SHECKPNGHE FMHDNKIITI FSASNYYAIG SNKGAYIRLN NQLMPHFVQY ISAASQTKRL 370 380 390 400 410 420 SFKQRMGIVE SSALKELAVR MRDHRDELED EFRKYDPKDS GYISISHWCK VMENVTKLGL 430 440 450 460 470 480 PWRLLRDKLA PGTDSQKVNY NRTLDLLDTD VILEAEADGM SVMDALYANK ASLVAIFNII 490 500 510 520 530 540 DADNSGEITL DEFETAIDLL VAHMPGAYSK AEMLEKCRMM DLNGDGKVDL NEFLEAFRLS 550 560 570 580 590 600 DLHRKEQQDE NIRRRSTGRP SVAKTATDPV TLLADKISKN TLVVEHDIDP TDCESKVIDP KKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Drosophila retinal degeneration C (rdgC) encodes a novel serine/threonine protein phosphatase." Steele F.R., Washburn T., Rieger R., O'Tousa J.E. Cell 69:669-676(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM B). Strain: Oregon-R.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[4]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Head.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M89628 Genomic DNA. Translation: AAB00734.1. AE014296 Genomic DNA. Translation: AAF49044.2. AE014296 Genomic DNA. Translation: AAO41217.1. AE014296 Genomic DNA. Translation: AAO41218.1. AE014296 Genomic DNA. Translation: AAO41219.1. BT015959 mRNA. Translation: AAV36844.1.
PIR	A42287.
RefSeq	NP_536738.2. NM_080490.3. NP_788544.1. NM_176366.1. NP_788545.2. NM_176367.2.
UniGene	Dm.5730.
3D structure databases
ProteinModelPortal	P40421.
SMR	P40421. Positions 115-410, 444-595.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-6825050.
Proteomic databases
PaxDb	P40421.
PRIDE	P40421.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0074833; FBpp0074602; FBgn0004366.
GeneID	40224.
KEGG	dme:Dmel_CG6571.
Organism-specific databases
CTD	40224.
FlyBase	FBgn0004366. rdgC.
Phylogenomic databases
eggNOG	COG0639.
GeneTree	ENSGT00530000063173.
InParanoid	P40421.
KO	K13807.
OMA	NMLEYKS.
OrthoDB	EOG42V6X7.
PhylomeDB	P40421.
Enzyme and pathway databases
SignaLink	P40421.
Gene expression databases
Bgee	P40421.
GermOnline	CG6571. Drosophila melanogaster.
Family and domain databases
Gene3D	1.10.238.10. 2 hits.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. IPR000048. IQ_motif_EF-hand-BS. IPR004843. Metallo_PEstase_dom. IPR012008. Ser/Thr-Pase_EF-hand_contain. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF13499. EF_hand_5. 1 hit. PF00149. Metallophos. 1 hit. [Graphical view]
PIRSF	PIRSF000912. PPEF. 1 hit.
PRINTS	PR00114. STPHPHTASE.
SMART	SM00054. EFh. 3 hits. SM00015. IQ. 1 hit. SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 3 hits. PS50096. IQ. 1 hit. PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	40224.
NextBio	817660.

Entry information

Entry name

RDGC_DROME

Accession

Primary (citable) accession number: P40421
Secondary accession number(s): A4V255, Q5U1D3, Q9VWA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 29, 2013
This is version 128 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project