Reviewed,
UniProtKB/Swiss-Prot P40417 (ERKA_DROME)
Last modified
November 3, 2009.
Version 98.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Mitogen-activated protein kinase ERK-A EC=2.7.11.24 Alternative name(s): Extracellular-regulated kinase A Protein rolled | ||||||
| Gene names |
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| Organism | Drosophila melanogaster (Fruit fly) [Complete proteome] | ||||||
| Taxonomic identifier | 7227 [NCBI] | ||||||
| Taxonomic lineage | Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora |
Protein attributes
| Sequence length | 426 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Required downstream of Raf in the sevenless (sev), torso (tor), and Drosophila EGF receptor homolog (DER) signal transduction pathways. Ref.7 |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Cofactor | Magnesium By similarity. |
| Enzyme regulation | Activated by tyrosine and threonine phosphorylation By similarity. |
| Subcellular location | |
| Tissue specificity | In third instar larvae, expressed in eye imaginal disks. In adults, expressed in head and body. |
| Developmental stage | Embryos, larvae and adults. Ref.1 |
| Domain | The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. |
| Post-translational modification | Dually phosphorylated on Thr-248 and Tyr-250, which activates the enzyme By similarity. Phosphorylated on tyrosine residue(s) in response to insulin. |
| Sequence similarities | Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| itself | 1 | EBI-867790,EBI-867790 | ||
| Ccp84Ad | O97061 | 1 | EBI-867790,EBI-188987 | |
| mael | Q9VNS0 | 1 | EBI-867790,EBI-140243 | |
| Mekk1 | Q9VE37 | 1 | EBI-867790,EBI-108552 | |
| Sas-4 | Q9VI72 | 1 | EBI-867790,EBI-131879 |
Alternative products
| This entry describes 3 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform F (identifier: P40417-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Note: No experimental confirmation available. | ||||||
| Isoform B (identifier: P40417-2) The sequence of this isoform differs from the canonical sequence as follows: 21-39: Missing. | ||||||
| Note: No experimental confirmation available. | ||||||
| Isoform C (identifier: P40417-3) Also known as: D; E; The sequence of this isoform differs from the canonical sequence as follows: 1-50: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 426 | 426 | Mitogen-activated protein kinase ERK-A | PRO_0000186309 | |||||
Regions | |||||||||
| Domain | 88 – 376 | 289 | Protein kinase | ||||||
| Nucleotide binding | 94 – 102 | 9 | ATP By similarity | ||||||
| Motif | 248 – 250 | 3 | TXY | ||||||
Sites | |||||||||
| Active site | 212 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 117 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 248 | 1 | Phosphothreonine Ref.8 | ||||||
| Modified residue | 250 | 1 | Phosphotyrosine Ref.8 | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 50 | 50 | Missing in isoform C. | VSP_032691 | |||||
| Alternative sequence | 21 – 39 | 19 | Missing in isoform B. | VSP_032692 | |||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Primary structure, expression, and signal-dependent tyrosine phosphorylation of a Drosophila homolog of extracellular signal-regulated kinase." Biggs W.H. III, Zipursky S.L. Proc. Natl. Acad. Sci. U.S.A. 89:6295-6299(1992) [PubMed: 1378625] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C), DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, PHOSPHORYLATION. Strain: Oregon-R. Tissue: Embryo and Imaginal disk. |
| [2] | Erratum Biggs W.H. III, Zipursky S.L. Proc. Natl. Acad. Sci. U.S.A. 90:6377-6377(1993) |
| [3] | "The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. Venter J.C.Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley. |
| [4] | "Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. Lewis S.E.Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. |
| [5] | "Heterochromatic sequences in a Drosophila whole-genome shotgun assembly." Hoskins R.A., Smith C.D., Carlson J.W., Carvalho A.B., Halpern A., Kaminker J.S., Kennedy C., Mungall C.J., Sullivan B.A., Sutton G.G., Yasuhara J.C., Wakimoto B.T., Myers E.W., Celniker S.E., Rubin G.M., Karpen G.H. Genome Biol. 3:RESEARCH0085.1-RESEARCH0085.16(2002) [PubMed: 12537574] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley. |
| [6] | "A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C). Strain: Berkeley. Tissue: Embryo. |
| [7] | "The Drosophila rolled locus encodes a MAP kinase required in the sevenless signal transduction pathway." Biggs W.H. III, Zavitz K.H., Dickson B., van der Straten A., Brunner D., Hafen E., Zipursky S.L. EMBO J. 13:1628-1635(1994) [PubMed: 8157002] [Abstract] Cited for: FUNCTION. Strain: Oregon-R. |
| [8] | "Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-248 AND TYR-250, MASS SPECTROMETRY. Tissue: Embryo. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| M95124 mRNA. Translation: AAA28677.1. CM000457 Genomic DNA. Translation: EAA46310.2. CM000457 Genomic DNA. Translation: EAA46311.2. CM000457 Genomic DNA. Translation: EAA46312.3. CM000457 Genomic DNA. Translation: EDP28106.1. CM000457 Genomic DNA. Translation: EDP28107.1. AY070996 mRNA. Translation: AAL48618.1. | |
| PIR | A46036. B46036. |
| RefSeq | NP_001015121.2. NP_001104348.1. NP_001104349.1. |
| UniGene | Dm.20303 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1PME based on UniProtKB P28482. |
| SMR | P40417. Positions 25-368. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP:17266N. |
| IntAct | P40417. 15 interactions. |
| STRING | P40417. |
Genome annotation databases | |
| Ensembl | FBtr0113699; FBpp0112422; FBgn0003256; Drosophila melanogaster. [Genome view] |
| GeneID | 3354888. |
| KEGG | dme:Dmel_CG12559. |
Organism-specific databases | |
| CTD | 3354888. |
| FlyBase | FBgn0003256. rl. |
Phylogenomic databases | |
| HOGENOM | P40417. |
| OMA | LAREDTA. |
Enzyme and pathway databases | |
| BRENDA | 2.7.11.24. 48. |
Gene expression databases | |
| GermOnline | CG12559. Drosophila melanogaster. |
Family and domain databases | |
| InterPro | IPR008349. Erk_1_2_MAPK. IPR003527. MAP_kinase_CS. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view] |
| Pfam | PF00069. Pkinase. 1 hit. [Graphical view] |
| PRINTS | PR01770. ERK1ERK2MAPK. |
| ProDom | PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00220. S_TKc. 1 hit. [Graphical view] |
| PROSITE | PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 849506. |
Entry information
| Entry name | ERKA_DROME | ||||||||
| Accession | Primary (citable) accession number: P40417 Secondary accession number(s): A8Y4W1 Q9W5M3 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | Drosophila annotation project | ||||||||
Relevant documents
| Drosophila Drosophila: entries, gene names and cross-references to FlyBase |
| SIMILARITY comments Index of protein domains and families |

Clusters with


