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P40416 (ATM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Iron-sulfur clusters transporter ATM1, mitochondrial
Gene names
Name:ATM1
Synonyms:MDY
Ordered Locus Names:YMR301C
ORF Names:YM9952.03C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length690 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Performs an essential function in the generation of cytoplasmic iron-sulfur proteins by mediating the ATP-dependent export of Fe/S cluster precursors synthesized by NFS1 and other mitochondrial proteins. Hydrolyzes ATP. Binds glutathione and may function by transporting a glutathione-conjugated iron-sulfur compound. Ref.5 Ref.8

Subunit structure

Homodimer. Ref.7 Ref.8

Subcellular location

Mitochondrion inner membrane; Multi-pass membrane protein Ref.1 Ref.8.

Miscellaneous

Present with 3250 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the ABC transporter superfamily. ABCB family. Heavy Metal importer (TC 3.A.1.210) subfamily. [View classification]

Contains 1 ABC transmembrane type-1 domain.

Contains 1 ABC transporter domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2626Mitochondrion Potential
Chain27 – 690664Iron-sulfur clusters transporter ATM1, mitochondrial
PRO_0000000258

Regions

Topological domain27 – 11084Mitochondrial matrix Ref.1 Ref.8
Transmembrane111 – 13222Helical
Topological domain133 – 15523Mitochondrial intermembrane Ref.1 Ref.8
Transmembrane156 – 17924Helical
Topological domain180 – 22849Mitochondrial matrix Ref.1 Ref.8
Transmembrane229 – 25224Helical
Topological domain2531Mitochondrial intermembrane Ref.1 Ref.8
Transmembrane254 – 27421Helical
Topological domain275 – 34066Mitochondrial matrix Ref.1 Ref.8
Transmembrane341 – 35919Helical
Topological domain360 – 37415Mitochondrial intermembrane Ref.1 Ref.8
Transmembrane375 – 39622Helical
Topological domain397 – 690294Mitochondrial matrix Ref.1 Ref.8
Domain111 – 401291ABC transmembrane type-1
Domain436 – 672237ABC transporter
Nucleotide binding469 – 48012ATP By similarity
Region280 – 2845Glutathione binding
Region343 – 3464Glutathione binding By similarity

Sites

Binding site3931Glutathione; via amide nitrogen By similarity
Binding site4451ATP By similarity

Experimental info

Mutagenesis4751K → M: Loss of function; significant decrease in ATP-binding; no homodimerization. Ref.7
Mutagenesis5981E → A: Loss of function; slight decrease in ATP-binding. Ref.7
Mutagenesis666 – 69025Missing: Impairs protein stability. Ref.8
Sequence conflict231I → IRNHS in CAA57938. Ref.1

Secondary structure

................................................................................... 690
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P40416 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 009757811D331343

FASTA69077,522
        10         20         30         40         50         60 
MLLLPRCPVI GRIVRSKFRS GLIRNHSPVI FTVSKLSTQR PLLFNSAVNL WNQAQKDITH 

        70         80         90        100        110        120 
KKSVEQFSSA PKVKTQVKKT SKAPTLSELK ILKDLFRYIW PKGNNKVRIR VLIALGLLIS 

       130        140        150        160        170        180 
AKILNVQVPF FFKQTIDSMN IAWDDPTVAL PAAIGLTILC YGVARFGSVL FGELRNAVFA 

       190        200        210        220        230        240 
KVAQNAIRTV SLQTFQHLMK LDLGWHLSRQ TGGLTRAMDR GTKGISQVLT AMVFHIIPIS 

       250        260        270        280        290        300 
FEISVVCGIL TYQFGASFAA ITFSTMLLYS IFTIKTTAWR THFRRDANKA DNKAASVALD 

       310        320        330        340        350        360 
SLINFEAVKY FNNEKYLADK YNGSLMNYRD SQIKVSQSLA FLNSGQNLIF TTALTAMMYM 

       370        380        390        400        410        420 
GCTGVIGGNL TVGDLVLINQ LVFQLSVPLN FLGSVYRDLK QSLIDMETLF KLRKNEVKIK 

       430        440        450        460        470        480 
NAERPLMLPE NVPYDITFEN VTFGYHPDRK ILKNASFTIP AGWKTAIVGS SGSGKSTILK 

       490        500        510        520        530        540 
LVFRFYDPES GRILINGRDI KEYDIDALRK VIGVVPQDTP LFNDTIWENV KFGRIDATDE 

       550        560        570        580        590        600 
EVITVVEKAQ LAPLIKKLPQ GFDTIVGERG LMISGGEKQR LAIARVLLKN ARIMFFDEAT 

       610        620        630        640        650        660 
SALDTHTEQA LLRTIRDNFT SGSRTSVYIA HRLRTIADAD KIIVLDNGRV REEGKHLELL 

       670        680        690 
AMPGSLYREL WTIQEDLDHL ENELKDQQEL 

« Hide

References

« Hide 'large scale' references
[1]"An ABC transporter in the mitochondrial inner membrane is required for normal growth of yeast."
Leighton J., Schatz G.
EMBO J. 14:188-195(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, TOPOLOGY.
Strain: JK9-3D alpha.
[2]"The ABC transporter Atm1p is required for mitochondrial iron homeostasis."
Kispal G., Csere P., Guiard B., Lill R.
FEBS Lett. 418:346-350(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"The mitochondrial proteins Atm1p and Nfs1p are essential for biogenesis of cytosolic Fe/S proteins."
Kispal G., Csere P., Prohl C., Lill R.
EMBO J. 18:3981-3989(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"The mitochondrial ABC transporter Atm1p functions as a homodimer."
Chloupkova M., Reaves S.K., LeBard L.M., Koeller D.M.
FEBS Lett. 569:65-69(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: HOMODIMERIZATION, MUTAGENESIS OF LYS-475 AND GLU-598.
[8]"Crystal structures of nucleotide-free and glutathione-bound mitochondrial ABC transporter Atm1."
Srinivasan V., Pierik A.J., Lill R.
Science 343:1137-1140(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.06 ANGSTROMS) OF 98-690 IN COMPLEX WITH GLUTATHIONE, FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, SUBUNIT, MUTAGENESIS OF 666-LEU--LEU-690.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X82612 Genomic DNA. Translation: CAA57938.1.
X81715 Genomic DNA. Translation: CAA57359.1.
Z49212 Genomic DNA. Translation: CAA89134.1.
BK006946 Genomic DNA. Translation: DAA10202.1.
PIRS54211.
RefSeqNP_014030.1. NM_001182810.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4MYCX-ray3.06A/B/C98-690[»]
4MYHX-ray3.38A/B/C98-690[»]
ProteinModelPortalP40416.
SMRP40416. Positions 98-690.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35481. 8 interactions.
IntActP40416. 8 interactions.
MINTMINT-1178130.
STRING4932.YMR301C.

Protein family/group databases

TCDB3.A.1.210.1. the atp-binding cassette (abc) superfamily.

Proteomic databases

MaxQBP40416.
PaxDbP40416.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR301C; YMR301C; YMR301C.
GeneID855347.
KEGGsce:YMR301C.

Organism-specific databases

CYGDYMR301c.
SGDS000004916. ATM1.

Phylogenomic databases

eggNOGCOG5265.
GeneTreeENSGT00440000033373.
KOK05663.
OMAQAIRYRV.
OrthoDBEOG74BK1Z.

Enzyme and pathway databases

BioCycYEAST:G3O-32967-MONOMER.
ReactomeREACT_160098. Cytosolic iron-sulfur cluster assembly (yeast).
REACT_191296. Metabolism.

Gene expression databases

GenevestigatorP40416.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
PROSITEPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979097.
PROP40416.

Entry information

Entry nameATM1_YEAST
AccessionPrimary (citable) accession number: P40416
Secondary accession number(s): D6W0C8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references