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P40416

- ATM1_YEAST

UniProt

P40416 - ATM1_YEAST

Protein

Iron-sulfur clusters transporter ATM1, mitochondrial

Gene

ATM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Performs an essential function in the generation of cytoplasmic iron-sulfur proteins by mediating the ATP-dependent export of Fe/S cluster precursors synthesized by NFS1 and other mitochondrial proteins. Hydrolyzes ATP. Binds glutathione and may function by transporting a glutathione-conjugated iron-sulfur compound.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei393 – 3931Glutathione; via amide nitrogenBy similarity
    Binding sitei445 – 4451ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi469 – 48012ATPPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATPase activity Source: SGD
    2. ATPase activity, coupled to transmembrane movement of substances Source: SGD
    3. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. cellular iron ion homeostasis Source: SGD
    3. ion transport Source: UniProtKB-KW

    Keywords - Biological processi

    Ion transport, Iron transport, Transport

    Keywords - Ligandi

    ATP-binding, Iron, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32967-MONOMER.
    ReactomeiREACT_188901. Cytosolic iron-sulfur cluster assembly.
    REACT_189255. Mitochondrial ABC transporters.

    Protein family/group databases

    TCDBi3.A.1.210.1. the atp-binding cassette (abc) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Iron-sulfur clusters transporter ATM1, mitochondrial
    Gene namesi
    Name:ATM1
    Synonyms:MDY
    Ordered Locus Names:YMR301C
    ORF Names:YM9952.03C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR301c.
    SGDiS000004916. ATM1.

    Subcellular locationi

    Mitochondrion inner membrane 2 Publications; Multi-pass membrane protein 2 PublicationsPROSITE-ProRule annotation

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrial inner membrane Source: SGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi475 – 4751K → M: Loss of function; significant decrease in ATP-binding; no homodimerization. 2 Publications
    Mutagenesisi598 – 5981E → A: Loss of function; slight decrease in ATP-binding. 2 Publications
    Mutagenesisi666 – 69025Missing: Impairs protein stability. 1 PublicationAdd
    BLAST

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2626MitochondrionSequence AnalysisAdd
    BLAST
    Chaini27 – 690664Iron-sulfur clusters transporter ATM1, mitochondrialPRO_0000000258Add
    BLAST

    Proteomic databases

    MaxQBiP40416.
    PaxDbiP40416.

    Expressioni

    Gene expression databases

    GenevestigatoriP40416.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    BioGridi35481. 8 interactions.
    IntActiP40416. 8 interactions.
    MINTiMINT-1178130.
    STRINGi4932.YMR301C.

    Structurei

    Secondary structure

    1
    690
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi103 – 1053
    Helixi106 – 1094
    Helixi110 – 12617
    Helixi128 – 13710
    Helixi152 – 19847
    Helixi203 – 2086
    Helixi212 – 2154
    Turni216 – 22813
    Helixi229 – 2346
    Helixi236 – 25419
    Helixi256 – 30348
    Helixi305 – 3106
    Helixi315 – 36652
    Beta strandi367 – 3704
    Helixi373 – 38210
    Helixi383 – 3853
    Helixi387 – 3893
    Turni390 – 3934
    Helixi397 – 41115
    Turni412 – 4143
    Beta strandi430 – 4323
    Beta strandi436 – 4438
    Beta strandi451 – 4599
    Beta strandi464 – 4685
    Helixi475 – 4773
    Helixi478 – 4825
    Beta strandi489 – 4957
    Helixi500 – 5023
    Helixi505 – 5106
    Beta strandi512 – 5154
    Beta strandi523 – 5253
    Helixi526 – 5305
    Helixi531 – 5333
    Helixi539 – 54810
    Helixi552 – 5576
    Helixi561 – 5633
    Beta strandi565 – 5673
    Helixi575 – 58915
    Beta strandi592 – 5987
    Helixi605 – 61814
    Beta strandi620 – 6234
    Beta strandi625 – 6317
    Helixi633 – 6364
    Beta strandi640 – 6467
    Beta strandi649 – 6546
    Helixi656 – 6616
    Helixi666 – 69025

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4MYCX-ray3.06A/B/C98-690[»]
    4MYHX-ray3.38A/B/C98-690[»]
    ProteinModelPortaliP40416.
    SMRiP40416. Positions 98-690.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini27 – 11084Mitochondrial matrixAdd
    BLAST
    Topological domaini133 – 15523Mitochondrial intermembraneAdd
    BLAST
    Topological domaini180 – 22849Mitochondrial matrixAdd
    BLAST
    Topological domaini253 – 2531Mitochondrial intermembrane
    Topological domaini275 – 34066Mitochondrial matrixAdd
    BLAST
    Topological domaini360 – 37415Mitochondrial intermembraneAdd
    BLAST
    Topological domaini397 – 690294Mitochondrial matrixAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei111 – 13222HelicalAdd
    BLAST
    Transmembranei156 – 17924HelicalAdd
    BLAST
    Transmembranei229 – 25224HelicalAdd
    BLAST
    Transmembranei254 – 27421HelicalAdd
    BLAST
    Transmembranei341 – 35919HelicalAdd
    BLAST
    Transmembranei375 – 39622HelicalAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini111 – 401291ABC transmembrane type-1PROSITE-ProRule annotationAdd
    BLAST
    Domaini436 – 672237ABC transporterPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni280 – 2845Glutathione binding
    Regioni343 – 3464Glutathione bindingBy similarity

    Sequence similaritiesi

    Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation
    Contains 1 ABC transporter domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5265.
    GeneTreeiENSGT00440000033373.
    KOiK05663.
    OMAiQAIRYRV.
    OrthoDBiEOG74BK1Z.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR011527. ABC1_TM_dom.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR001140. ABC_transptr_TM_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00664. ABC_membrane. 1 hit.
    PF00005. ABC_tran. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    SSF90123. SSF90123. 1 hit.
    PROSITEiPS50929. ABC_TM1F. 1 hit.
    PS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P40416-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLLLPRCPVI GRIVRSKFRS GLIRNHSPVI FTVSKLSTQR PLLFNSAVNL    50
    WNQAQKDITH KKSVEQFSSA PKVKTQVKKT SKAPTLSELK ILKDLFRYIW 100
    PKGNNKVRIR VLIALGLLIS AKILNVQVPF FFKQTIDSMN IAWDDPTVAL 150
    PAAIGLTILC YGVARFGSVL FGELRNAVFA KVAQNAIRTV SLQTFQHLMK 200
    LDLGWHLSRQ TGGLTRAMDR GTKGISQVLT AMVFHIIPIS FEISVVCGIL 250
    TYQFGASFAA ITFSTMLLYS IFTIKTTAWR THFRRDANKA DNKAASVALD 300
    SLINFEAVKY FNNEKYLADK YNGSLMNYRD SQIKVSQSLA FLNSGQNLIF 350
    TTALTAMMYM GCTGVIGGNL TVGDLVLINQ LVFQLSVPLN FLGSVYRDLK 400
    QSLIDMETLF KLRKNEVKIK NAERPLMLPE NVPYDITFEN VTFGYHPDRK 450
    ILKNASFTIP AGWKTAIVGS SGSGKSTILK LVFRFYDPES GRILINGRDI 500
    KEYDIDALRK VIGVVPQDTP LFNDTIWENV KFGRIDATDE EVITVVEKAQ 550
    LAPLIKKLPQ GFDTIVGERG LMISGGEKQR LAIARVLLKN ARIMFFDEAT 600
    SALDTHTEQA LLRTIRDNFT SGSRTSVYIA HRLRTIADAD KIIVLDNGRV 650
    REEGKHLELL AMPGSLYREL WTIQEDLDHL ENELKDQQEL 690
    Length:690
    Mass (Da):77,522
    Last modified:February 1, 1996 - v2
    Checksum:i009757811D331343
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti23 – 231I → IRNHS in CAA57938. (PubMed:7828591)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X82612 Genomic DNA. Translation: CAA57938.1.
    X81715 Genomic DNA. Translation: CAA57359.1.
    Z49212 Genomic DNA. Translation: CAA89134.1.
    BK006946 Genomic DNA. Translation: DAA10202.1.
    PIRiS54211.
    RefSeqiNP_014030.1. NM_001182810.1.

    Genome annotation databases

    EnsemblFungiiYMR301C; YMR301C; YMR301C.
    GeneIDi855347.
    KEGGisce:YMR301C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X82612 Genomic DNA. Translation: CAA57938.1 .
    X81715 Genomic DNA. Translation: CAA57359.1 .
    Z49212 Genomic DNA. Translation: CAA89134.1 .
    BK006946 Genomic DNA. Translation: DAA10202.1 .
    PIRi S54211.
    RefSeqi NP_014030.1. NM_001182810.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4MYC X-ray 3.06 A/B/C 98-690 [» ]
    4MYH X-ray 3.38 A/B/C 98-690 [» ]
    ProteinModelPortali P40416.
    SMRi P40416. Positions 98-690.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35481. 8 interactions.
    IntActi P40416. 8 interactions.
    MINTi MINT-1178130.
    STRINGi 4932.YMR301C.

    Protein family/group databases

    TCDBi 3.A.1.210.1. the atp-binding cassette (abc) superfamily.

    Proteomic databases

    MaxQBi P40416.
    PaxDbi P40416.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR301C ; YMR301C ; YMR301C .
    GeneIDi 855347.
    KEGGi sce:YMR301C.

    Organism-specific databases

    CYGDi YMR301c.
    SGDi S000004916. ATM1.

    Phylogenomic databases

    eggNOGi COG5265.
    GeneTreei ENSGT00440000033373.
    KOi K05663.
    OMAi QAIRYRV.
    OrthoDBi EOG74BK1Z.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32967-MONOMER.
    Reactomei REACT_188901. Cytosolic iron-sulfur cluster assembly.
    REACT_189255. Mitochondrial ABC transporters.

    Miscellaneous databases

    NextBioi 979097.
    PROi P40416.

    Gene expression databases

    Genevestigatori P40416.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR011527. ABC1_TM_dom.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR001140. ABC_transptr_TM_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00664. ABC_membrane. 1 hit.
    PF00005. ABC_tran. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    SSF90123. SSF90123. 1 hit.
    PROSITEi PS50929. ABC_TM1F. 1 hit.
    PS00211. ABC_TRANSPORTER_1. 1 hit.
    PS50893. ABC_TRANSPORTER_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "An ABC transporter in the mitochondrial inner membrane is required for normal growth of yeast."
      Leighton J., Schatz G.
      EMBO J. 14:188-195(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, TOPOLOGY.
      Strain: JK9-3D alpha.
    2. "The ABC transporter Atm1p is required for mitochondrial iron homeostasis."
      Kispal G., Csere P., Guiard B., Lill R.
      FEBS Lett. 418:346-350(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "The mitochondrial proteins Atm1p and Nfs1p are essential for biogenesis of cytosolic Fe/S proteins."
      Kispal G., Csere P., Prohl C., Lill R.
      EMBO J. 18:3981-3989(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "The mitochondrial ABC transporter Atm1p functions as a homodimer."
      Chloupkova M., Reaves S.K., LeBard L.M., Koeller D.M.
      FEBS Lett. 569:65-69(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: HOMODIMERIZATION, MUTAGENESIS OF LYS-475 AND GLU-598.
    8. "Crystal structures of nucleotide-free and glutathione-bound mitochondrial ABC transporter Atm1."
      Srinivasan V., Pierik A.J., Lill R.
      Science 343:1137-1140(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.06 ANGSTROMS) OF 98-690 IN COMPLEX WITH GLUTATHIONE, FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, SUBUNIT, MUTAGENESIS OF 666-LEU--LEU-690.

    Entry informationi

    Entry nameiATM1_YEAST
    AccessioniPrimary (citable) accession number: P40416
    Secondary accession number(s): D6W0C8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 135 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3250 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3