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P40416

- ATM1_YEAST

UniProt

P40416 - ATM1_YEAST

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Protein

Iron-sulfur clusters transporter ATM1, mitochondrial

Gene

ATM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Performs an essential function in the generation of cytoplasmic iron-sulfur proteins by mediating the ATP-dependent export of Fe/S cluster precursors synthesized by NFS1 and other mitochondrial proteins. Hydrolyzes ATP. Binds glutathione and may function by transporting a glutathione-conjugated iron-sulfur compound.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei393 – 3931Glutathione; via amide nitrogenBy similarity
Binding sitei445 – 4451ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi469 – 48012ATPPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATPase activity Source: SGD
  2. ATPase activity, coupled to transmembrane movement of substances Source: SGD
  3. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. cellular iron ion homeostasis Source: SGD
  3. ion transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

ATP-binding, Iron, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32967-MONOMER.
ReactomeiREACT_188901. Cytosolic iron-sulfur cluster assembly.
REACT_189255. Mitochondrial ABC transporters.

Protein family/group databases

TCDBi3.A.1.210.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Iron-sulfur clusters transporter ATM1, mitochondrial
Gene namesi
Name:ATM1
Synonyms:MDY
Ordered Locus Names:YMR301C
ORF Names:YM9952.03C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR301c.
SGDiS000004916. ATM1.

Subcellular locationi

Mitochondrion inner membrane 2 Publications; Multi-pass membrane protein 2 PublicationsPROSITE-ProRule annotation

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial inner membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi475 – 4751K → M: Loss of function; significant decrease in ATP-binding; no homodimerization. 1 Publication
Mutagenesisi598 – 5981E → A: Loss of function; slight decrease in ATP-binding. 1 Publication
Mutagenesisi666 – 69025Missing: Impairs protein stability. 1 PublicationAdd
BLAST

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2626MitochondrionSequence AnalysisAdd
BLAST
Chaini27 – 690664Iron-sulfur clusters transporter ATM1, mitochondrialPRO_0000000258Add
BLAST

Proteomic databases

MaxQBiP40416.
PaxDbiP40416.

Expressioni

Gene expression databases

GenevestigatoriP40416.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi35481. 8 interactions.
IntActiP40416. 8 interactions.
MINTiMINT-1178130.
STRINGi4932.YMR301C.

Structurei

Secondary structure

1
690
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi103 – 1053
Helixi106 – 1094
Helixi110 – 12617
Helixi128 – 13710
Helixi152 – 19847
Helixi203 – 2086
Helixi212 – 2154
Turni216 – 22813
Helixi229 – 2346
Helixi236 – 25419
Helixi256 – 30348
Helixi305 – 3106
Helixi315 – 36652
Beta strandi367 – 3704
Helixi373 – 38210
Helixi383 – 3853
Helixi387 – 3893
Turni390 – 3934
Helixi397 – 41115
Turni412 – 4143
Beta strandi430 – 4323
Beta strandi436 – 4438
Beta strandi451 – 4599
Beta strandi464 – 4685
Helixi475 – 4773
Helixi478 – 4825
Beta strandi489 – 4957
Helixi500 – 5023
Helixi505 – 5106
Beta strandi512 – 5154
Beta strandi523 – 5253
Helixi526 – 5305
Helixi531 – 5333
Helixi539 – 54810
Helixi552 – 5576
Helixi561 – 5633
Beta strandi565 – 5673
Helixi575 – 58915
Beta strandi592 – 5987
Helixi605 – 61814
Beta strandi620 – 6234
Beta strandi625 – 6317
Helixi633 – 6364
Beta strandi640 – 6467
Beta strandi649 – 6546
Helixi656 – 6616
Helixi666 – 69025

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4MYCX-ray3.06A/B/C98-690[»]
4MYHX-ray3.38A/B/C98-690[»]
ProteinModelPortaliP40416.
SMRiP40416. Positions 98-690.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini27 – 11084Mitochondrial matrixAdd
BLAST
Topological domaini133 – 15523Mitochondrial intermembraneAdd
BLAST
Topological domaini180 – 22849Mitochondrial matrixAdd
BLAST
Topological domaini253 – 2531Mitochondrial intermembrane
Topological domaini275 – 34066Mitochondrial matrixAdd
BLAST
Topological domaini360 – 37415Mitochondrial intermembraneAdd
BLAST
Topological domaini397 – 690294Mitochondrial matrixAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei111 – 13222HelicalAdd
BLAST
Transmembranei156 – 17924HelicalAdd
BLAST
Transmembranei229 – 25224HelicalAdd
BLAST
Transmembranei254 – 27421HelicalAdd
BLAST
Transmembranei341 – 35919HelicalAdd
BLAST
Transmembranei375 – 39622HelicalAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini111 – 401291ABC transmembrane type-1PROSITE-ProRule annotationAdd
BLAST
Domaini436 – 672237ABC transporterPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni280 – 2845Glutathione binding
Regioni343 – 3464Glutathione bindingBy similarity

Sequence similaritiesi

Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation
Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG5265.
GeneTreeiENSGT00440000033373.
InParanoidiP40416.
KOiK05663.
OMAiQAIRYRV.
OrthoDBiEOG74BK1Z.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40416-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLLLPRCPVI GRIVRSKFRS GLIRNHSPVI FTVSKLSTQR PLLFNSAVNL
60 70 80 90 100
WNQAQKDITH KKSVEQFSSA PKVKTQVKKT SKAPTLSELK ILKDLFRYIW
110 120 130 140 150
PKGNNKVRIR VLIALGLLIS AKILNVQVPF FFKQTIDSMN IAWDDPTVAL
160 170 180 190 200
PAAIGLTILC YGVARFGSVL FGELRNAVFA KVAQNAIRTV SLQTFQHLMK
210 220 230 240 250
LDLGWHLSRQ TGGLTRAMDR GTKGISQVLT AMVFHIIPIS FEISVVCGIL
260 270 280 290 300
TYQFGASFAA ITFSTMLLYS IFTIKTTAWR THFRRDANKA DNKAASVALD
310 320 330 340 350
SLINFEAVKY FNNEKYLADK YNGSLMNYRD SQIKVSQSLA FLNSGQNLIF
360 370 380 390 400
TTALTAMMYM GCTGVIGGNL TVGDLVLINQ LVFQLSVPLN FLGSVYRDLK
410 420 430 440 450
QSLIDMETLF KLRKNEVKIK NAERPLMLPE NVPYDITFEN VTFGYHPDRK
460 470 480 490 500
ILKNASFTIP AGWKTAIVGS SGSGKSTILK LVFRFYDPES GRILINGRDI
510 520 530 540 550
KEYDIDALRK VIGVVPQDTP LFNDTIWENV KFGRIDATDE EVITVVEKAQ
560 570 580 590 600
LAPLIKKLPQ GFDTIVGERG LMISGGEKQR LAIARVLLKN ARIMFFDEAT
610 620 630 640 650
SALDTHTEQA LLRTIRDNFT SGSRTSVYIA HRLRTIADAD KIIVLDNGRV
660 670 680 690
REEGKHLELL AMPGSLYREL WTIQEDLDHL ENELKDQQEL
Length:690
Mass (Da):77,522
Last modified:February 1, 1996 - v2
Checksum:i009757811D331343
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti23 – 231I → IRNHS in CAA57938. (PubMed:7828591)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X82612 Genomic DNA. Translation: CAA57938.1.
X81715 Genomic DNA. Translation: CAA57359.1.
Z49212 Genomic DNA. Translation: CAA89134.1.
BK006946 Genomic DNA. Translation: DAA10202.1.
PIRiS54211.
RefSeqiNP_014030.1. NM_001182810.1.

Genome annotation databases

EnsemblFungiiYMR301C; YMR301C; YMR301C.
GeneIDi855347.
KEGGisce:YMR301C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X82612 Genomic DNA. Translation: CAA57938.1 .
X81715 Genomic DNA. Translation: CAA57359.1 .
Z49212 Genomic DNA. Translation: CAA89134.1 .
BK006946 Genomic DNA. Translation: DAA10202.1 .
PIRi S54211.
RefSeqi NP_014030.1. NM_001182810.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4MYC X-ray 3.06 A/B/C 98-690 [» ]
4MYH X-ray 3.38 A/B/C 98-690 [» ]
ProteinModelPortali P40416.
SMRi P40416. Positions 98-690.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35481. 8 interactions.
IntActi P40416. 8 interactions.
MINTi MINT-1178130.
STRINGi 4932.YMR301C.

Protein family/group databases

TCDBi 3.A.1.210.1. the atp-binding cassette (abc) superfamily.

Proteomic databases

MaxQBi P40416.
PaxDbi P40416.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR301C ; YMR301C ; YMR301C .
GeneIDi 855347.
KEGGi sce:YMR301C.

Organism-specific databases

CYGDi YMR301c.
SGDi S000004916. ATM1.

Phylogenomic databases

eggNOGi COG5265.
GeneTreei ENSGT00440000033373.
InParanoidi P40416.
KOi K05663.
OMAi QAIRYRV.
OrthoDBi EOG74BK1Z.

Enzyme and pathway databases

BioCyci YEAST:G3O-32967-MONOMER.
Reactomei REACT_188901. Cytosolic iron-sulfur cluster assembly.
REACT_189255. Mitochondrial ABC transporters.

Miscellaneous databases

NextBioi 979097.
PROi P40416.

Gene expression databases

Genevestigatori P40416.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
PROSITEi PS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "An ABC transporter in the mitochondrial inner membrane is required for normal growth of yeast."
    Leighton J., Schatz G.
    EMBO J. 14:188-195(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, TOPOLOGY.
    Strain: JK9-3D alpha.
  2. "The ABC transporter Atm1p is required for mitochondrial iron homeostasis."
    Kispal G., Csere P., Guiard B., Lill R.
    FEBS Lett. 418:346-350(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "The mitochondrial proteins Atm1p and Nfs1p are essential for biogenesis of cytosolic Fe/S proteins."
    Kispal G., Csere P., Prohl C., Lill R.
    EMBO J. 18:3981-3989(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "The mitochondrial ABC transporter Atm1p functions as a homodimer."
    Chloupkova M., Reaves S.K., LeBard L.M., Koeller D.M.
    FEBS Lett. 569:65-69(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: HOMODIMERIZATION, MUTAGENESIS OF LYS-475 AND GLU-598.
  8. "Crystal structures of nucleotide-free and glutathione-bound mitochondrial ABC transporter Atm1."
    Srinivasan V., Pierik A.J., Lill R.
    Science 343:1137-1140(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.06 ANGSTROMS) OF 98-690 IN COMPLEX WITH GLUTATHIONE, FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, SUBUNIT, MUTAGENESIS OF 666-LEU--LEU-690.

Entry informationi

Entry nameiATM1_YEAST
AccessioniPrimary (citable) accession number: P40416
Secondary accession number(s): D6W0C8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3250 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3