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P40416 (ATM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Iron-sulfur clusters transporter ATM1, mitochondrial
Gene names
Name:ATM1
Synonyms:MDY
Ordered Locus Names:YMR301C
ORF Names:YM9952.03C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length690 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Performs an essential function in the generation of cytoplasmic iron-sulfur proteins by mediating export of Fe/S cluster precursors synthesized by NFS1 and other mitochondrial proteins. Ref.5

Subunit structure

Homodimer. Ref.7

Subcellular location

Mitochondrion inner membrane; Multi-pass membrane protein Ref.1.

Miscellaneous

Present with 3250 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the ABC transporter superfamily. ABCB family. Heavy Metal importer (TC 3.A.1.210) subfamily. [View classification]

Contains 1 ABC transmembrane type-1 domain.

Contains 1 ABC transporter domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2626Mitochondrion Potential
Chain27 – 690664Iron-sulfur clusters transporter ATM1, mitochondrial
PRO_0000000258

Regions

Topological domain27 – 11185Mitochondrial matrix Potential
Transmembrane112 – 13221Helical; Potential
Topological domain133 – 15119Mitochondrial intermembrane Potential
Transmembrane152 – 17221Helical; Potential
Topological domain173 – 22856Mitochondrial matrix Potential
Transmembrane229 – 24921Helical; Potential
Topological domain250 – 2534Mitochondrial intermembrane Potential
Transmembrane254 – 27421Helical; Potential
Topological domain275 – 34773Mitochondrial matrix Potential
Transmembrane348 – 36821Helical; Potential
Topological domain369 – 3746Mitochondrial intermembrane Potential
Transmembrane375 – 39521Helical; Potential
Topological domain396 – 690295Mitochondrial matrix Potential
Domain111 – 401291ABC transmembrane type-1
Domain436 – 672237ABC transporter
Nucleotide binding469 – 4768ATP Potential

Experimental info

Mutagenesis4751K → M: Loss of function; significant decrease in ATP-binding; no homodimerization. Ref.7
Mutagenesis5981E → A: Loss of function; slight decrease in ATP-binding. Ref.7
Sequence conflict231I → IRNHS in CAA57938. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P40416 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 009757811D331343

FASTA69077,522
        10         20         30         40         50         60 
MLLLPRCPVI GRIVRSKFRS GLIRNHSPVI FTVSKLSTQR PLLFNSAVNL WNQAQKDITH 

        70         80         90        100        110        120 
KKSVEQFSSA PKVKTQVKKT SKAPTLSELK ILKDLFRYIW PKGNNKVRIR VLIALGLLIS 

       130        140        150        160        170        180 
AKILNVQVPF FFKQTIDSMN IAWDDPTVAL PAAIGLTILC YGVARFGSVL FGELRNAVFA 

       190        200        210        220        230        240 
KVAQNAIRTV SLQTFQHLMK LDLGWHLSRQ TGGLTRAMDR GTKGISQVLT AMVFHIIPIS 

       250        260        270        280        290        300 
FEISVVCGIL TYQFGASFAA ITFSTMLLYS IFTIKTTAWR THFRRDANKA DNKAASVALD 

       310        320        330        340        350        360 
SLINFEAVKY FNNEKYLADK YNGSLMNYRD SQIKVSQSLA FLNSGQNLIF TTALTAMMYM 

       370        380        390        400        410        420 
GCTGVIGGNL TVGDLVLINQ LVFQLSVPLN FLGSVYRDLK QSLIDMETLF KLRKNEVKIK 

       430        440        450        460        470        480 
NAERPLMLPE NVPYDITFEN VTFGYHPDRK ILKNASFTIP AGWKTAIVGS SGSGKSTILK 

       490        500        510        520        530        540 
LVFRFYDPES GRILINGRDI KEYDIDALRK VIGVVPQDTP LFNDTIWENV KFGRIDATDE 

       550        560        570        580        590        600 
EVITVVEKAQ LAPLIKKLPQ GFDTIVGERG LMISGGEKQR LAIARVLLKN ARIMFFDEAT 

       610        620        630        640        650        660 
SALDTHTEQA LLRTIRDNFT SGSRTSVYIA HRLRTIADAD KIIVLDNGRV REEGKHLELL 

       670        680        690 
AMPGSLYREL WTIQEDLDHL ENELKDQQEL 

« Hide

References

« Hide 'large scale' references
[1]"An ABC transporter in the mitochondrial inner membrane is required for normal growth of yeast."
Leighton J., Schatz G.
EMBO J. 14:188-195(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, TOPOLOGY.
Strain: JK9-3D alpha.
[2]"The ABC transporter Atm1p is required for mitochondrial iron homeostasis."
Kispal G., Csere P., Guiard B., Lill R.
FEBS Lett. 418:346-350(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"The mitochondrial proteins Atm1p and Nfs1p are essential for biogenesis of cytosolic Fe/S proteins."
Kispal G., Csere P., Prohl C., Lill R.
EMBO J. 18:3981-3989(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"The mitochondrial ABC transporter Atm1p functions as a homodimer."
Chloupkova M., Reaves S.K., LeBard L.M., Koeller D.M.
FEBS Lett. 569:65-69(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: HOMODIMERIZATION, MUTAGENESIS OF LYS-475 AND GLU-598.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X82612 Genomic DNA. Translation: CAA57938.1.
X81715 Genomic DNA. Translation: CAA57359.1.
Z49212 Genomic DNA. Translation: CAA89134.1.
BK006946 Genomic DNA. Translation: DAA10202.1.
PIRS54211.
RefSeqNP_014030.1. NM_001182810.1.

3D structure databases

ProteinModelPortalP40416.
SMRP40416. Positions 108-675.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35481. 8 interactions.
IntActP40416. 8 interactions.
MINTMINT-1178130.
STRING4932.YMR301C.

Protein family/group databases

TCDB3.A.1.210.1. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbP40416.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR301C; YMR301C; YMR301C.
GeneID855347.
KEGGsce:YMR301C.

Organism-specific databases

CYGDYMR301c.
SGDS000004916. ATM1.

Phylogenomic databases

eggNOGCOG5265.
GeneTreeENSGT00440000033373.
KOK05663.
OMAVGQHAVR.
OrthoDBEOG74BK1Z.

Enzyme and pathway databases

BioCycYEAST:G3O-32967-MONOMER.
ReactomeREACT_160098. Cytosolic Iron-sulfur Cluster Assembly (yeast).
REACT_191296. Metabolism.

Gene expression databases

GenevestigatorP40416.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
PROSITEPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio979097.
PROP40416.

Entry information

Entry nameATM1_YEAST
AccessionPrimary (citable) accession number: P40416
Secondary accession number(s): D6W0C8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families