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P40413 (TCPE_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
T-complex protein 1 subunit epsilon

Short name=TCP-1-epsilon
Alternative name(s):
CCT-epsilon
Gene names
Name:CCT5
Synonyms:TCP5
Ordered Locus Names:YJR064W
ORF Names:J1752
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length562 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin. In yeast may play a role in mitotic spindle formation.

Subunit structure

Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the TCP-1 chaperonin family.

Sequence caution

The sequence AAA53132.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionChaperone
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein folding

Inferred from direct assay PubMed 16762366. Source: SGD

   Cellular_componentchaperonin-containing T-complex

Inferred from direct assay PubMed 16762366. Source: SGD

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

unfolded protein binding

Inferred from direct assay PubMed 16762366. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 562562T-complex protein 1 subunit epsilon
PRO_0000128354

Experimental info

Sequence conflict31A → T in AAA53132. Ref.1
Sequence conflict271R → T in AAA53132. Ref.1
Sequence conflict581A → D in AAA53132. Ref.1
Sequence conflict921S → T in AAA53132. Ref.1
Sequence conflict274 – 2796CPFEPP → VHLNLL in AAA53132. Ref.1
Sequence conflict3361L → I in AAA53132. Ref.1
Sequence conflict4881G → N in AAA53132. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P40413 [UniParc].

Last modified December 12, 2006. Version 3.
Checksum: 8CDAF88C6403655F

FASTA56261,914
        10         20         30         40         50         60 
MAARPQQPPM EMPDLSNAIV AQDEMGRPFI IVKDQGNKKR QHGLEAKKSH ILAARSVASI 

        70         80         90        100        110        120 
IKTSLGPRGL DKILISPDGE ITITNDGATI LSQMELDNEI AKLLVQLSKS QDDEIGDGTT 

       130        140        150        160        170        180 
GVVVLASALL DQALELIQKG IHPIKIANGF DEAAKLAISK LEETCDDISA SNDELFRDFL 

       190        200        210        220        230        240 
LRAAKTSLGS KIVSKDHDRF AEMAVEAVIN VMDKDRKDVD FDLIKMQGRV GGSISDSKLI 

       250        260        270        280        290        300 
NGVILDKDFS HPQMPKCVLP KEGSDGVKLA ILTCPFEPPK PKTKHKLDIS SVEEYQKLQT 

       310        320        330        340        350        360 
YEQDKFKEMI DDVKKAGADV VICQWGFDDE ANHLLLQNDL PAVRWVGGQE LEHIAISTNG 

       370        380        390        400        410        420 
RIVPRFQDLS KDKLGTCSRI YEQEFGTTKD RMLIIEQSKE TKTVTCFVRG SNKMIVDEAE 

       430        440        450        460        470        480 
RALHDSLCVV RNLVKDSRVV YGGGAAEVTM SLAVSEEADK QRGIDQYAFR GFAQALDTIP 

       490        500        510        520        530        540 
MTLAENSGLD PIGTLSTLKS KQLKEKISNI GVDCLGYGSN DMKELFVVDP FIGKKQQILL 

       550        560 
ATQLCRMILK IDNVIISGKD EY 

« Hide

References

« Hide 'large scale' references
[1]"Cytosolic chaperonin(CCT) subunits have a conserved ATPase domain but diverged polypeptide-binding domains."
Kim S.
Submitted (NOV-1994) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: YPH501.
[2]"Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI."
Huang M.-E., Manus V., Chuat J.-C., Galibert F.
Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L37350 Genomic DNA. Translation: AAA53132.1. Different initiation.
L47993 Genomic DNA. Translation: AAB39290.1.
Z49564 Genomic DNA. Translation: CAA89592.1.
BK006943 Genomic DNA. Translation: DAA08851.1.
PIRS57083.
RefSeqNP_012598.1. NM_001181722.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3P9DX-ray3.80E/M1-562[»]
3P9EX-ray3.80e/m1-562[»]
4AOLX-ray3.80E/e1-562[»]
4APKX-ray3.80E/e1-562[»]
4D8QX-ray3.80E/M1-562[»]
4D8RX-ray3.80e/m1-562[»]
ProteinModelPortalP40413.
SMRP40413. Positions 25-559.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33821. 38 interactions.
DIPDIP-2205N.
IntActP40413. 18 interactions.
MINTMINT-636377.
STRING4932.YJR064W.

Proteomic databases

MaxQBP40413.
PaxDbP40413.
PeptideAtlasP40413.
PRIDEP40413.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJR064W; YJR064W; YJR064W.
GeneID853527.
KEGGsce:YJR064W.

Organism-specific databases

CYGDYJR064w.
SGDS000003825. CCT5.

Phylogenomic databases

eggNOGCOG0459.
GeneTreeENSGT00550000074988.
HOGENOMHOG000226735.
KOK09497.
OMAYATDEYG.
OrthoDBEOG773XR5.

Enzyme and pathway databases

BioCycYEAST:G3O-31697-MONOMER.

Gene expression databases

GenevestigatorP40413.

Family and domain databases

Gene3D1.10.560.10. 2 hits.
3.30.260.10. 2 hits.
3.50.7.10. 1 hit.
InterProIPR012718. Chap_CCT_epsi.
IPR017998. Chaperone_TCP-1.
IPR002194. Chaperonin_TCP-1_CS.
IPR002423. Cpn60/TCP-1.
IPR027409. GroEL-like_apical_dom.
IPR027413. GROEL-like_equatorial.
IPR027410. TCP-1-like_intermed.
[Graphical view]
PANTHERPTHR11353. PTHR11353. 1 hit.
PfamPF00118. Cpn60_TCP1. 1 hit.
[Graphical view]
PRINTSPR00304. TCOMPLEXTCP1.
SUPFAMSSF48592. SSF48592. 2 hits.
SSF52029. SSF52029. 1 hit.
TIGRFAMsTIGR02343. chap_CCT_epsi. 1 hit.
PROSITEPS00750. TCP1_1. 1 hit.
PS00995. TCP1_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974218.
PROP40413.

Entry information

Entry nameTCPE_YEAST
AccessionPrimary (citable) accession number: P40413
Secondary accession number(s): D6VWN5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: December 12, 2006
Last modified: June 11, 2014
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references