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P40399

- RSBU_BACSU

UniProt

P40399 - RSBU_BACSU

Protein

Phosphoserine phosphatase RsbU

Gene

rsbU

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 3 (15 Jul 1998)
      Previous versions | rss
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    Functioni

    Positive regulator of sigma-B activity. Dephosphorylates RsbV in response to environmental stress conveyed from the RsbXST module.1 Publication

    Catalytic activityi

    O-phospho-L(or D)-serine + H2O = L(or D)-serine + phosphate.

    Enzyme regulationi

    Stimulated by a long-lived interaction with RsbT.1 Publication

    GO - Molecular functioni

    1. phosphoprotein phosphatase activity Source: UniProtKB-KW
    2. phosphoserine phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protein phosphorylation Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Enzyme and pathway databases

    BioCyciBSUB:BSU04700-MONOMER.

    Protein family/group databases

    PptaseDBiP3D0406128.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoserine phosphatase RsbU (EC:3.1.3.3)
    Alternative name(s):
    Sigma factor SigB regulation protein RsbU
    Gene namesi
    Name:rsbU
    Ordered Locus Names:BSU04700
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU04700. [Micado]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 335335Phosphoserine phosphatase RsbUPRO_0000057790Add
    BLAST

    Proteomic databases

    PaxDbiP40399.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-410N.
    STRINGi224308.BSU04700.

    Structurei

    Secondary structure

    1
    335
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi2 – 2120
    Helixi24 – 3916
    Helixi44 – 5815
    Helixi64 – 8320

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1W53X-ray1.60A1-84[»]
    2J6YX-ray1.85A/B/C/D/E1-111[»]
    2J6ZX-ray1.95A1-111[»]
    2J70X-ray1.95A1-111[»]
    ProteinModelPortaliP40399.
    SMRiP40399. Positions 1-84, 107-334.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP40399.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini131 – 335205PP2C-likeAdd
    BLAST

    Sequence similaritiesi

    Contains 1 PP2C-like domain.Curated

    Phylogenomic databases

    eggNOGiCOG2208.
    HOGENOMiHOG000269934.
    KOiK07315.
    OMAiSIHRKVL.
    OrthoDBiEOG6ZH2CT.
    PhylomeDBiP40399.

    Family and domain databases

    Gene3Di1.10.1240.30. 1 hit.
    3.60.40.10. 1 hit.
    InterProiIPR017944. KaiA/RbsU_helical_domain.
    IPR001932. PP2C-like_dom.
    IPR014787. PSer_Pase_RsbU_N.
    [Graphical view]
    PfamiPF08673. RsbU_N. 1 hit.
    PF07228. SpoIIE. 1 hit.
    [Graphical view]
    ProDomiPD685490. PSer_Pase_RsbU_N. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view]
    SUPFAMiSSF101215. SSF101215. 1 hit.
    SSF81606. SSF81606. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P40399-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDFREVIEQR YHQLLSRYIA ELTETSLYQA QKFSRKTIEH QIPPEEIISI    50
    HRKVLKELYP SLPEDVFHSL DFLIEVMIGY GMAYQEHQTL RGIQQEIKSE 100
    IEIAANVQQT LLGTKVPQEE ALDIGAISVP AKQMSGDYYH FVKDKESINI 150
    AIADVIGKGI PAALCMSMIK YAMDSLPETG IHPSQVLKNL NRVVEQNVDA 200
    SMFITMFYAN YNMDKHQFTY ASAGHEPGFY YSQKDNTFYD LEAKGLVLGI 250
    SQDYDYKQFD QHLEKGDMIV LFSDGVTECR TENGFLERPD LQKLIEEHMC 300
    SSAQEMVKNI YDSLLKLQDF QLHDDFTLIV LRRKV 335
    Length:335
    Mass (Da):38,631
    Last modified:July 15, 1998 - v3
    Checksum:i5BD37C05B1A930E3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti323 – 3231H → Q(PubMed:8002610)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L35574 Genomic DNA. Translation: AAA85083.1.
    X81652 Genomic DNA. Translation: CAB57214.1. Sequence problems.
    AB001488 Genomic DNA. Translation: BAA19307.1.
    AL009126 Genomic DNA. Translation: CAB12277.1.
    PIRiH69701.
    RefSeqiNP_388351.1. NC_000964.3.
    WP_003234295.1. NZ_CM000487.1.

    Genome annotation databases

    EnsemblBacteriaiCAB12277; CAB12277; BSU04700.
    GeneIDi939939.
    KEGGibsu:BSU04700.
    PATRICi18972524. VBIBacSub10457_0490.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L35574 Genomic DNA. Translation: AAA85083.1 .
    X81652 Genomic DNA. Translation: CAB57214.1 . Sequence problems.
    AB001488 Genomic DNA. Translation: BAA19307.1 .
    AL009126 Genomic DNA. Translation: CAB12277.1 .
    PIRi H69701.
    RefSeqi NP_388351.1. NC_000964.3.
    WP_003234295.1. NZ_CM000487.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1W53 X-ray 1.60 A 1-84 [» ]
    2J6Y X-ray 1.85 A/B/C/D/E 1-111 [» ]
    2J6Z X-ray 1.95 A 1-111 [» ]
    2J70 X-ray 1.95 A 1-111 [» ]
    ProteinModelPortali P40399.
    SMRi P40399. Positions 1-84, 107-334.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-410N.
    STRINGi 224308.BSU04700.

    Protein family/group databases

    PptaseDBi P3D0406128.

    Proteomic databases

    PaxDbi P40399.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB12277 ; CAB12277 ; BSU04700 .
    GeneIDi 939939.
    KEGGi bsu:BSU04700.
    PATRICi 18972524. VBIBacSub10457_0490.

    Organism-specific databases

    GenoListi BSU04700. [Micado ]

    Phylogenomic databases

    eggNOGi COG2208.
    HOGENOMi HOG000269934.
    KOi K07315.
    OMAi SIHRKVL.
    OrthoDBi EOG6ZH2CT.
    PhylomeDBi P40399.

    Enzyme and pathway databases

    BioCyci BSUB:BSU04700-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P40399.

    Family and domain databases

    Gene3Di 1.10.1240.30. 1 hit.
    3.60.40.10. 1 hit.
    InterProi IPR017944. KaiA/RbsU_helical_domain.
    IPR001932. PP2C-like_dom.
    IPR014787. PSer_Pase_RsbU_N.
    [Graphical view ]
    Pfami PF08673. RsbU_N. 1 hit.
    PF07228. SpoIIE. 1 hit.
    [Graphical view ]
    ProDomi PD685490. PSer_Pase_RsbU_N. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00331. PP2C_SIG. 1 hit.
    SM00332. PP2Cc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF101215. SSF101215. 1 hit.
    SSF81606. SSF81606. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Four additional genes in the sigB operon of Bacillus subtilis that control activity of the general stress factor sigma B in response to environmental signals."
      Wise A.A., Price C.W.
      J. Bacteriol. 177:123-133(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
    2. "The Bacillus subtilis rsbU gene product is necessary for RsbX-dependent regulation of sigma B."
      Voelker U., Dufour A., Haldenwang W.G.
      J. Bacteriol. 177:114-122(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 / BSA46.
    3. "A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus subtilis genome."
      Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N.
      Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    4. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    5. "Opposing pairs of serine protein kinases and phosphatases transmit signals of environmental stress to activate a bacterial transcription factor."
      Yang X., Kang C.M., Brody M.S., Price C.W.
      Genes Dev. 10:2265-2275(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
    6. "Serine kinase activity of a Bacillus subtilis switch protein is required to transduce environmental stress signals but not to activate its target PP2C phosphatase."
      Kang C.M., Vijay K., Price C.W.
      Mol. Microbiol. 30:189-196(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: ENZYME REGULATION.
      Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
    7. "Crystallization and preliminary crystallographic analysis of the kinase-recruitment domain of the PP2C-type phosphatase RsbU."
      Dutta S., Lewis R.J.
      Acta Crystallogr. D 59:191-193(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: CRYSTALLIZATION OF N-TERMINAL DOMAIN, PROTEIN SEQUENCE OF 112-121.

    Entry informationi

    Entry nameiRSBU_BACSU
    AccessioniPrimary (citable) accession number: P40399
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: July 15, 1998
    Last modified: October 1, 2014
    This is version 117 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3