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Protein

Probable thiamine biosynthetic bifunctional enzyme

Gene

thi4

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP).By similarity

Catalytic activityi

4-amino-2-methyl-5-diphosphomethylpyrimidine + 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate = diphosphate + thiamine phosphate + CO2.
4-amino-2-methyl-5-diphosphomethylpyrimidine + 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate = diphosphate + thiamine phosphate + CO2.
4-amino-2-methyl-5-diphosphomethylpyrimidine + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate.
ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.
Proteins known to be involved in this subpathway in this organism are:
  1. Probable thiamine biosynthetic bifunctional enzyme (thi4)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole.
Proteins known to be involved in this subpathway in this organism are:
  1. Probable thiamine biosynthetic bifunctional enzyme (thi4)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei72HMP-PPBy similarity1
Metal bindingi73MagnesiumBy similarity1
Metal bindingi92MagnesiumBy similarity1
Binding sitei111HMP-PPBy similarity1
Binding sitei140HMP-PPBy similarity1
Binding sitei173THZ-P; via amide nitrogenBy similarity1
Binding sitei2814-methyl-5-(2-hydroxyethyl)thiazole; via amide nitrogenBy similarity1
Binding sitei355ATPBy similarity1
Binding sitei403ATPBy similarity1
Binding sitei4304-methyl-5-(2-hydroxyethyl)thiazole; via amide nitrogenBy similarity1
Active sitei433Proton acceptor; for hydroxyethylthiazole kinase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.
UPA00060; UER00141.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable thiamine biosynthetic bifunctional enzyme
Including the following 2 domains:
Thiamine-phosphate synthase (EC:2.5.1.3)
Short name:
TP synthase
Short name:
TPS
Alternative name(s):
Thiamine-phosphate pyrophosphorylase
Short name:
TMP pyrophosphorylase
Short name:
TMP-PPase
Hydroxyethylthiazole kinase (EC:2.7.1.50)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name:
TH kinase
Short name:
THZ kinase
Gene namesi
Name:thi4
ORF Names:SPAC23H4.10c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC23H4.10c.
PomBaseiSPAC23H4.10c. thi4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001570891 – 518Probable thiamine biosynthetic bifunctional enzymeAdd BLAST518

Proteomic databases

MaxQBiP40386.
PRIDEiP40386.

Expressioni

Inductioni

Repressed by thiamine and 5-(2-hydroxyethyl)-4-methylthiazole.

Interactioni

Protein-protein interaction databases

BioGridi278362. 11 interactors.
MINTiMINT-4689729.

Structurei

3D structure databases

ProteinModelPortaliP40386.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 229Thiamine-phosphate synthaseAdd BLAST229
Regioni40 – 44HMP-PP bindingBy similarity5
Regioni137 – 139THZ-P bindingBy similarity3
Regioni199 – 200THZ-P bindingBy similarity2
Regioni230 – 518Hydroxyethylthiazole kinaseAdd BLAST289

Sequence similaritiesi

In the N-terminal section; belongs to the thiamine-phosphate synthase family.Curated
In the C-terminal section; belongs to the Thz kinase family.Curated

Phylogenomic databases

HOGENOMiHOG000214306.
InParanoidiP40386.
KOiK14154.
OMAiNLVVQNF.
OrthoDBiEOG092C3IZ7.
PhylomeDBiP40386.

Family and domain databases

CDDicd01170. THZ_kinase. 1 hit.
cd00564. TMP_TenI. 1 hit.
Gene3Di3.20.20.70. 1 hit.
3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase. 1 hit.
MF_00097. TMP_synthase. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
IPR022998. ThiaminP_synth_SF.
IPR003733. TMP_synthase.
[Graphical view]
PfamiPF02110. HK. 1 hit.
PF02581. TMP-TENI. 1 hit.
[Graphical view]
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF51391. SSF51391. 1 hit.
SSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00693. thiE. 1 hit.
TIGR00694. thiM. 1 hit.

Sequencei

Sequence statusi: Complete.

P40386-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRQIDYSLY LVTSSSLIAP GSTIERQVEE GILGGVTLVQ HREKDISTKC
60 70 80 90 100
FVERAKRLSE ICKKYDVPFL INDRIDVALA VGADGVHIGQ DDMDCALARK
110 120 130 140 150
ILGDDAIIGV STNNIEEIEK AAADGADYVG IGSIYETNTK DVKDRLIGIT
160 170 180 190 200
GLRKILEHVS KMHCQLGTVA IAGLNSSNIQ RVIYLSEANG KRIDGIALVS
210 220 230 240 250
AIMCSITPRE TAKELRNLIA TPPCFAQARS SLTTPKDLLN QIPAALQKLK
260 270 280 290 300
DFTPLIHHLT NAVAKNFSAN VTLAAYGSPT MGESYDEVAD FAKAPGALVL
310 320 330 340 350
NIGILENTKT YIHAAQVNND LARPVILDPV AVGATTARSK VINTLLNYAY
360 370 380 390 400
YDIIKGNEGE IMNLAGEQGL MRGVDSISQH TLAARITAVH RLAVERRCVV
410 420 430 440 450
AMSGAVDVIS DGNSTYVIKN GNPLLGQITA SGCSLGSVMG VTASICQNDK
460 470 480 490 500
LLAAITATLL YNIASELAVE AKNSCGDLLV QGPGTFIPIF VDKLHQLINE
510
TIKGNVDWIE RAKLEKAE
Length:518
Mass (Da):55,566
Last modified:February 1, 1995 - v1
Checksum:iB8836D2BD67C79AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78824 Genomic DNA. Translation: CAA55402.1.
CU329670 Genomic DNA. Translation: CAB11664.1.
PIRiS44183.
RefSeqiNP_593396.1. NM_001018828.2.

Genome annotation databases

EnsemblFungiiSPAC23H4.10c.1; SPAC23H4.10c.1:pep; SPAC23H4.10c.
GeneIDi2541872.
KEGGispo:SPAC23H4.10c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78824 Genomic DNA. Translation: CAA55402.1.
CU329670 Genomic DNA. Translation: CAB11664.1.
PIRiS44183.
RefSeqiNP_593396.1. NM_001018828.2.

3D structure databases

ProteinModelPortaliP40386.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278362. 11 interactors.
MINTiMINT-4689729.

Proteomic databases

MaxQBiP40386.
PRIDEiP40386.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC23H4.10c.1; SPAC23H4.10c.1:pep; SPAC23H4.10c.
GeneIDi2541872.
KEGGispo:SPAC23H4.10c.

Organism-specific databases

EuPathDBiFungiDB:SPAC23H4.10c.
PomBaseiSPAC23H4.10c. thi4.

Phylogenomic databases

HOGENOMiHOG000214306.
InParanoidiP40386.
KOiK14154.
OMAiNLVVQNF.
OrthoDBiEOG092C3IZ7.
PhylomeDBiP40386.

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.
UPA00060; UER00141.

Miscellaneous databases

PROiP40386.

Family and domain databases

CDDicd01170. THZ_kinase. 1 hit.
cd00564. TMP_TenI. 1 hit.
Gene3Di3.20.20.70. 1 hit.
3.40.1190.20. 1 hit.
HAMAPiMF_00228. Thz_kinase. 1 hit.
MF_00097. TMP_synthase. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
IPR022998. ThiaminP_synth_SF.
IPR003733. TMP_synthase.
[Graphical view]
PfamiPF02110. HK. 1 hit.
PF02581. TMP-TENI. 1 hit.
[Graphical view]
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF51391. SSF51391. 1 hit.
SSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00693. thiE. 1 hit.
TIGR00694. thiM. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHI6_SCHPO
AccessioniPrimary (citable) accession number: P40386
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 5, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.