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P40386

- THI6_SCHPO

UniProt

P40386 - THI6_SCHPO

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Protein
Probable thiamine biosynthetic bifunctional enzyme
Gene
thi4, SPAC23H4.10c
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) By similarity.UniRule annotation

Catalytic activityi

2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate.UniRule annotation
ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei72 – 721HMP-PP By similarity
Metal bindingi73 – 731Magnesium By similarity
Metal bindingi92 – 921Magnesium By similarity
Binding sitei111 – 1111HMP-PP By similarity
Binding sitei140 – 1401HMP-PP By similarity
Binding sitei173 – 1731THZ-P; via amide nitrogen By similarity
Binding sitei281 – 28114-methyl-5-(2-hydroxyethyl)thiazole; via amide nitrogen By similarity
Binding sitei355 – 3551ATP By similarity
Binding sitei403 – 4031ATP By similarity
Binding sitei430 – 43014-methyl-5-(2-hydroxyethyl)thiazole; via amide nitrogen By similarity
Active sitei433 – 4331Proton acceptor; for hydroxyethylthiazole kinase activity By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydroxyethylthiazole kinase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. thiamine-phosphate diphosphorylase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. thiamine biosynthetic process Source: UniProtKB-KW
  2. thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00060; UER00139.
UPA00060; UER00141.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable thiamine biosynthetic bifunctional enzyme
Including the following 2 domains:
Thiamine-phosphate synthase (EC:2.5.1.3)
Short name:
TP synthase
Short name:
TPS
Alternative name(s):
Thiamine-phosphate pyrophosphorylase
Short name:
TMP pyrophosphorylase
Short name:
TMP-PPase
Hydroxyethylthiazole kinase (EC:2.7.1.50)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinase
Short name:
TH kinase
Short name:
THZ kinase
Gene namesi
Name:thi4
ORF Names:SPAC23H4.10c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC23H4.10c.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: PomBase
  2. cytosol Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 518518Probable thiamine biosynthetic bifunctional enzymeUniRule annotation
PRO_0000157089Add
BLAST

Proteomic databases

MaxQBiP40386.

Expressioni

Inductioni

Repressed by thiamine and 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation

Interactioni

Protein-protein interaction databases

BioGridi278362. 7 interactions.
MINTiMINT-4689729.
STRINGi4896.SPAC23H4.10c-1.

Structurei

3D structure databases

ProteinModelPortaliP40386.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 229229Thiamine-phosphate synthaseUniRule annotation
Add
BLAST
Regioni40 – 445HMP-PP binding By similarity
Regioni137 – 1393THZ-P binding By similarity
Regioni199 – 2002THZ-P binding By similarity
Regioni230 – 518289Hydroxyethylthiazole kinaseUniRule annotation
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the thiamine-phosphate synthase family.
In the C-terminal section; belongs to the Thz kinase family.

Phylogenomic databases

eggNOGiCOG0352.
HOGENOMiHOG000214306.
KOiK14154.
OMAiNLVVQNF.
OrthoDBiEOG7KDFMZ.
PhylomeDBiP40386.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
3.40.1190.20. 1 hit.
HAMAPiMF_00097. TMP_synthase.
MF_00228. Thz_kinase.
InterProiIPR013785. Aldolase_TIM.
IPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
IPR022998. ThiaminP_synth_SF.
IPR003733. TMP_synthase.
[Graphical view]
PfamiPF02110. HK. 1 hit.
PF02581. TMP-TENI. 1 hit.
[Graphical view]
PRINTSiPR01099. HYETHTZKNASE.
SUPFAMiSSF51391. SSF51391. 1 hit.
SSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00693. thiE. 1 hit.
TIGR00694. thiM. 1 hit.

Sequencei

Sequence statusi: Complete.

P40386-1 [UniParc]FASTAAdd to Basket

« Hide

MKRQIDYSLY LVTSSSLIAP GSTIERQVEE GILGGVTLVQ HREKDISTKC    50
FVERAKRLSE ICKKYDVPFL INDRIDVALA VGADGVHIGQ DDMDCALARK 100
ILGDDAIIGV STNNIEEIEK AAADGADYVG IGSIYETNTK DVKDRLIGIT 150
GLRKILEHVS KMHCQLGTVA IAGLNSSNIQ RVIYLSEANG KRIDGIALVS 200
AIMCSITPRE TAKELRNLIA TPPCFAQARS SLTTPKDLLN QIPAALQKLK 250
DFTPLIHHLT NAVAKNFSAN VTLAAYGSPT MGESYDEVAD FAKAPGALVL 300
NIGILENTKT YIHAAQVNND LARPVILDPV AVGATTARSK VINTLLNYAY 350
YDIIKGNEGE IMNLAGEQGL MRGVDSISQH TLAARITAVH RLAVERRCVV 400
AMSGAVDVIS DGNSTYVIKN GNPLLGQITA SGCSLGSVMG VTASICQNDK 450
LLAAITATLL YNIASELAVE AKNSCGDLLV QGPGTFIPIF VDKLHQLINE 500
TIKGNVDWIE RAKLEKAE 518
Length:518
Mass (Da):55,566
Last modified:February 1, 1995 - v1
Checksum:iB8836D2BD67C79AA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78824 Genomic DNA. Translation: CAA55402.1.
CU329670 Genomic DNA. Translation: CAB11664.1.
PIRiS44183.
RefSeqiNP_593396.1. NM_001018828.2.

Genome annotation databases

EnsemblFungiiSPAC23H4.10c.1; SPAC23H4.10c.1:pep; SPAC23H4.10c.
GeneIDi2541872.
KEGGispo:SPAC23H4.10c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78824 Genomic DNA. Translation: CAA55402.1 .
CU329670 Genomic DNA. Translation: CAB11664.1 .
PIRi S44183.
RefSeqi NP_593396.1. NM_001018828.2.

3D structure databases

ProteinModelPortali P40386.
ModBasei Search...

Protein-protein interaction databases

BioGridi 278362. 7 interactions.
MINTi MINT-4689729.
STRINGi 4896.SPAC23H4.10c-1.

Proteomic databases

MaxQBi P40386.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAC23H4.10c.1 ; SPAC23H4.10c.1:pep ; SPAC23H4.10c .
GeneIDi 2541872.
KEGGi spo:SPAC23H4.10c.

Organism-specific databases

PomBasei SPAC23H4.10c.

Phylogenomic databases

eggNOGi COG0352.
HOGENOMi HOG000214306.
KOi K14154.
OMAi NLVVQNF.
OrthoDBi EOG7KDFMZ.
PhylomeDBi P40386.

Enzyme and pathway databases

UniPathwayi UPA00060 ; UER00139 .
UPA00060 ; UER00141 .

Miscellaneous databases

NextBioi 20802959.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
3.40.1190.20. 1 hit.
HAMAPi MF_00097. TMP_synthase.
MF_00228. Thz_kinase.
InterProi IPR013785. Aldolase_TIM.
IPR000417. Hyethyz_kinase.
IPR029056. Ribokinase-like.
IPR022998. ThiaminP_synth_SF.
IPR003733. TMP_synthase.
[Graphical view ]
Pfami PF02110. HK. 1 hit.
PF02581. TMP-TENI. 1 hit.
[Graphical view ]
PRINTSi PR01099. HYETHTZKNASE.
SUPFAMi SSF51391. SSF51391. 1 hit.
SSF53613. SSF53613. 1 hit.
TIGRFAMsi TIGR00693. thiE. 1 hit.
TIGR00694. thiM. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, nucleotide sequence, and regulation of Schizosaccharomyces pombe thi4, a thiamine biosynthetic gene."
    Zurlinden A., Schweingruber M.E.
    J. Bacteriol. 176:6631-6635(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiTHI6_SCHPO
AccessioniPrimary (citable) accession number: P40386
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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