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Protein

Meiotic recombination protein rec12

Gene

rec12

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for crossover recombination and chiasmatic segregation of chromosomes during meiosis I. Also involved in the faithful equational segregation of chromosomes during meiosis II.2 Publications

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei98 – 981Nucleophile1 Publication
Metal bindingi179 – 1791MagnesiumBy similarity
Metal bindingi229 – 2291MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

  • chromosome segregation Source: UniProtKB-KW
  • DNA catabolic process, endonucleolytic Source: InterPro
  • meiotic DNA double-strand break formation Source: PomBase
  • reciprocal meiotic recombination Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Chromosome partition, Meiosis

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Meiotic recombination protein rec12
Gene namesi
Name:rec12
ORF Names:SPAC17A5.11
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC17A5.11.
PomBaseiSPAC17A5.11. rec12.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • nuclear chromosome Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi98 – 981Y → F: Errors in meiosis II segregation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 345345Meiotic recombination protein rec12PRO_0000145477Add
BLAST

Expressioni

Developmental stagei

Most abundant at 2 hours after induction of meiosis, at the time of premeiotic DNA synthesis. Found at much lower levels before and after this time.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi278626. 49 interactions.
MINTiMINT-4689701.

Structurei

3D structure databases

ProteinModelPortaliP40384.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TOP6A family.Curated

Phylogenomic databases

InParanoidiP40384.
KOiK10878.
OMAiSKANERQ.
OrthoDBiEOG7P2Z3N.
PhylomeDBiP40384.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR002815. Spo11/TopoVI_A.
IPR013049. Spo11/TopoVI_A_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10848. PTHR10848. 1 hit.
PfamiPF04406. TP6A_N. 1 hit.
[Graphical view]
PRINTSiPR01550. TOP6AFAMILY.
SUPFAMiSSF56726. SSF56726. 1 hit.

Sequencei

Sequence statusi: Complete.

P40384-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSNDKKKVV RSWIEQFVHD FVEQLSKPTK DSVNVALKRR KHNSWNGSLD
60 70 80 90 100
SKANERQKVK VFSFPRNETT IAQLFRVLDC VHEAVISDTV ITKRDIYYRD
110 120 130 140 150
VDLFKRQTVV DELLGDISNT IGCSRSDLNV EASAKGLVFG SIHIALENGT
160 170 180 190 200
VITATKPLLI SHHRISSITS TAKWVLVIEK EAVFQTLTEE ALADTIIVTA
210 220 230 240 250
KGFPDLMTRK FLVKLAKALP DAKFFGIFDW DPHGLCIYSC FKYGSNAYSH
260 270 280 290 300
EPHSQLRNLQ LLGPLYEDIF NKNQEFSLKL NKRDIKMITT LLQFEGFQKE
310 320 330 340
PVVREQLQRM LFIQKKAEIQ AILEFPSWIK GKLADADKSG KHSVR
Length:345
Mass (Da):39,399
Last modified:July 15, 1998 - v2
Checksum:i056257FAAE7A2781
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF195027 mRNA. Translation: AAF06017.1.
CU329670 Genomic DNA. Translation: CAB11511.1.
L14774 Genomic DNA. Translation: AAA35332.1.
PIRiT37826.
RefSeqiNP_593479.1. NM_001018912.1.

Genome annotation databases

EnsemblFungiiSPAC17A5.11.1; SPAC17A5.11.1:pep; SPAC17A5.11.
GeneIDi2542150.
KEGGispo:SPAC17A5.11.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF195027 mRNA. Translation: AAF06017.1.
CU329670 Genomic DNA. Translation: CAB11511.1.
L14774 Genomic DNA. Translation: AAA35332.1.
PIRiT37826.
RefSeqiNP_593479.1. NM_001018912.1.

3D structure databases

ProteinModelPortaliP40384.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278626. 49 interactions.
MINTiMINT-4689701.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC17A5.11.1; SPAC17A5.11.1:pep; SPAC17A5.11.
GeneIDi2542150.
KEGGispo:SPAC17A5.11.

Organism-specific databases

EuPathDBiFungiDB:SPAC17A5.11.
PomBaseiSPAC17A5.11. rec12.

Phylogenomic databases

InParanoidiP40384.
KOiK10878.
OMAiSKANERQ.
OrthoDBiEOG7P2Z3N.
PhylomeDBiP40384.

Miscellaneous databases

NextBioi20803222.
PROiP40384.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR002815. Spo11/TopoVI_A.
IPR013049. Spo11/TopoVI_A_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10848. PTHR10848. 1 hit.
PfamiPF04406. TP6A_N. 1 hit.
[Graphical view]
PRINTSiPR01550. TOP6AFAMILY.
SUPFAMiSSF56726. SSF56726. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Distinct functions of S. pombe Rec12 (Spo11) protein and Rec12-dependent crossover recombination (chiasmata) in meiosis I; and a requirement for Rec12 in meiosis II."
    Sharif W.D., Glick G.G., Davidson M.K., Wahls W.P.
    Cell Chromosome 1:1-1(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF TYR-98.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Transient, meiosis-induced expression of the rec6 and rec12 genes of Schizosaccharomyces pombe."
    Lin Y., Smith G.R.
    Genetics 136:769-779(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 207-335, DEVELOPMENTAL STAGE.
  4. "A large-scale screen in S. pombe identifies seven novel genes required for critical meiotic events."
    Martin-Castellanos C., Blanco M., Rozalen A.E., Perez-Hidalgo L., Garcia A.I., Conde F., Mata J., Ellermeier C., Davis L., San-Segundo P., Smith G.R., Moreno S.
    Curr. Biol. 15:2056-2062(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN MEIOSIS.
  5. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiREC12_SCHPO
AccessioniPrimary (citable) accession number: P40384
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 15, 1998
Last modified: March 16, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.