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P40380

- RUM1_SCHPO

UniProt

P40380 - RUM1_SCHPO

Protein

Cyclin-dependent kinase inhibitor rum1

Gene

rum1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 2 (11 Jan 2001)
      Previous versions | rss
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    Functioni

    Regulator of cell cycle G1 phase progression. Ensures the correct sequence of S phase and mitosis in the cell by acting as an inhibitor of the cdc2 mitotic kinase. Probably interacts with cdc2 to inhibit its action until the cell mass for Start is reached. Determines the length of the pre-Start G1 period and prevents mitosis from happening in early G1 cells. Required for maintaining pheromone-induced G1 arrest. Acts as an adapter protein since interaction with cdc13 promotes cyclin proteolysis during G1. Becomes a target for degradation at the G1/S phase transition, following phosphorylation by cig1-associated cdc2 at the G1/S phase transition.8 Publications

    GO - Molecular functioni

    1. cyclin-dependent protein serine/threonine kinase inhibitor activity Source: PomBase
    2. protein binding Source: IntAct

    GO - Biological processi

    1. cell cycle arrest in response to nitrogen starvation Source: PomBase
    2. cell cycle arrest in response to pheromone Source: PomBase
    3. cellular response to nitrogen starvation Source: PomBase
    4. cyclin catabolic process Source: PomBase
    5. G1 cell size control checkpoint Source: PomBase
    6. negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: PomBase
    7. negative regulation of protein phosphorylation Source: PomBase
    8. regulation of DNA-dependent DNA replication initiation Source: PomBase
    9. signal transduction Source: PomBase

    Keywords - Biological processi

    Cell cycle

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase inhibitor rum1
    Alternative name(s):
    p25-rum1
    Gene namesi
    Name:rum1
    ORF Names:SPBC32F12.09
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome II

    Organism-specific databases

    PomBaseiSPBC32F12.09.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: PomBase

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi5 – 51T → A: No effect on phenotype and no reduction in 32-P incorporation mediated by MAPK. 2 Publications
    Mutagenesisi13 – 131T → A: No effect on phenotype; when associated with A-16 and A-19. Reduced 32-P incorporation mediated by MAPK; when associated with A-19. 2 Publications
    Mutagenesisi13 – 131T → E: Reduces activity as a cdc2 inhibitor; when associated with E-19. 2 Publications
    Mutagenesisi16 – 161T → A: No effect on phenotype; when associated with A-13 and A-19. No reduction in 32-P incorporation mediated by MAPK. 2 Publications
    Mutagenesisi19 – 191S → A: No effect on phenotype; when associated with A-13 and A-16. Reduced 32-P incorporation mediated by MAPK; when associated with A-13. 2 Publications
    Mutagenesisi19 – 191S → E: Reduces activity as a cdc2 inhibitor; when associated with E-13. 2 Publications
    Mutagenesisi58 – 581T → A: Reduces growth rate into small colonies containing many elongated cells. Phenotype enhances due to increased stability; when associated with A-62. No reduction in 32-P incorporation mediated by MAPK. 2 Publications
    Mutagenesisi58 – 581T → S: Shift in mobility; when associated with S-62. 2 Publications
    Mutagenesisi62 – 621T → A: Reduces growth rate into small colonies containing many elongated cells. Phenotype enhances due to increased stability; when associated with A-58. 1 Publication
    Mutagenesisi62 – 621T → S: Shift in mobility; when associated with S-58. 1 Publication
    Mutagenesisi110 – 1101T → A: No effect on phenotype. 1 Publication
    Mutagenesisi212 – 2121S → A: No effect on phenotype. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 230230Cyclin-dependent kinase inhibitor rum1PRO_0000097531Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei13 – 131Phosphothreonine; by MAPK2 Publications
    Modified residuei19 – 191Phosphoserine; by MAPK2 Publications
    Modified residuei58 – 581Phosphothreonine; by cdc22 Publications
    Modified residuei62 – 621Phosphothreonine; by cdc22 Publications

    Post-translational modificationi

    Phosphorylated by cig1-associated cdc2 which leads to increased stability. Phosphorylation by MAPK reduces cdc2 kinase inhibitor ability.3 Publications

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Inductioni

    Expression increases in nitrogen deprived environment, which is important to cause delay of the G1 phase of the cell cycle in response to nitrogen starvation.1 Publication

    Interactioni

    Subunit structurei

    Interacts with cdc13, cig2 and pop1.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    cdc13P108155EBI-1187892,EBI-1187843
    cdc2P045512EBI-1187892,EBI-1187862
    cig2P366302EBI-1187892,EBI-1149212

    Protein-protein interaction databases

    BioGridi276195. 31 interactions.
    IntActiP40380. 5 interactions.
    MINTiMINT-4689673.
    STRINGi4896.SPBC32F12.09-1.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni67 – 14781CDK inhibitory and cyclin-bindingAdd
    BLAST
    Regioni101 – 230130Required for activity as a cdc2 kinase inhibitorAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi151 – 1544Poly-Ser
    Compositional biasi192 – 1976Poly-Ser

    Phylogenomic databases

    OMAiDICIFRR.

    Sequencei

    Sequence statusi: Complete.

    P40380-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEPSTPPMRG LCTPSTPESP GSFKGVIDAS LEGNSSIMID EIPESDLPAP    50
    QVSTFPPTPA KTPKKQLLPN LMLQDRSNSL ERCMEEDREH NPFLSSSDNQ 100
    LLSRKKRKPT PPPSDGLYYV FRGKRIKKSF RPGTDLSTFK PKLLFADSAP 150
    SSSSDNPTSS VDLNDYSQIG ILPPNLNSIG NKMFSLKSRV PSSSSGSFVA 200
    PPPQMRLPAY SSPQKSRSNT KDENRHNLLR 230
    Length:230
    Mass (Da):25,289
    Last modified:January 11, 2001 - v2
    Checksum:iC5199FE345F7484A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti72 – 732ML → IV in CAA54786. (PubMed:8121488)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77730 mRNA. Translation: CAA54786.1.
    CU329671 Genomic DNA. Translation: CAA19370.1.
    PIRiS41043.
    T40233.
    RefSeqiNP_596152.1. NM_001022071.2.

    Genome annotation databases

    EnsemblFungiiSPBC32F12.09.1; SPBC32F12.09.1:pep; SPBC32F12.09.
    GeneIDi2539640.
    KEGGispo:SPBC32F12.09.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77730 mRNA. Translation: CAA54786.1 .
    CU329671 Genomic DNA. Translation: CAA19370.1 .
    PIRi S41043.
    T40233.
    RefSeqi NP_596152.1. NM_001022071.2.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 276195. 31 interactions.
    IntActi P40380. 5 interactions.
    MINTi MINT-4689673.
    STRINGi 4896.SPBC32F12.09-1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPBC32F12.09.1 ; SPBC32F12.09.1:pep ; SPBC32F12.09 .
    GeneIDi 2539640.
    KEGGi spo:SPBC32F12.09.

    Organism-specific databases

    PomBasei SPBC32F12.09.

    Phylogenomic databases

    OMAi DICIFRR.

    Miscellaneous databases

    NextBioi 20800794.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Regulation of progression through the G1 phase of the cell cycle by the rum1+ gene."
      Moreno S., Nurse P.
      Nature 367:236-242(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
      Strain: 972 / ATCC 24843.
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. "p25rum1 orders S phase and mitosis by acting as an inhibitor of the p34cdc2 mitotic kinase."
      Correa-Bordes J., Nurse P.
      Cell 83:1001-1009(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "p25rum1 promotes proteolysis of the mitotic B-cyclin p56cdc13 during G1 of the fission yeast cell cycle."
      Correa-Bordes J., Gulli M.P., Nurse P.
      EMBO J. 16:4657-4664(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CDC13, PHOSPHORYLATION.
    5. "Regulation of the G1 phase of the cell cycle by periodic stabilization and degradation of the p25rum1 CDK inhibitor."
      Benito J., Martin-Castellanos C., Moreno S.
      EMBO J. 17:482-497(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION AT THR-58 AND THR-62, MUTAGENESIS OF THR-5; THR-13; THR-16; SER-19; THR-58; THR-62; THR-110 AND SER-212.
    6. "The Cdk inhibitors p25rum1 and p40SIC1 are functional homologues that play similar roles in the regulation of the cell cycle in fission and budding yeast."
      Sanchez-Diaz A., Gonzalez I., Arellano M., Moreno S.
      J. Cell Sci. 111:843-851(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CDC13, DOMAIN.
    7. "Cyclin B proteolysis and the cyclin-dependent kinase inhibitor rum1p are required for pheromone-induced G1 arrest in fission yeast."
      Stern B., Nurse P.
      Mol. Biol. Cell 9:1309-1321(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH CDC13 AND CIG2.
    8. "Rum1, an inhibitor of cyclin-dependent kinase in fission yeast, is negatively regulated by mitogen-activated protein kinase-mediated phosphorylation at Ser and Thr residues."
      Matsuoka K., Kiyokawa N., Taguchi T., Matsui J., Suzuki T., Mimori K., Nakajima H., Takenouchi H., Weiran T., Katagiri Y.U., Fujimoto J.
      Eur. J. Biochem. 269:3511-3521(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION AT THR-13 AND SER-19, MUTAGENESIS OF THR-5; THR-13; THR-16; SER-19 AND THR-58.
    9. "Regulated mRNA stability of the Cdk inhibitor Rum1 links nutrient status to cell cycle progression."
      Daga R.R., Bolanos P., Moreno S.
      Curr. Biol. 13:2015-2024(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.
    10. "Requirement of the SCFPop1/Pop2 ubiquitin ligase for degradation of the fission yeast S phase cyclin Cig2."
      Yamano H., Kominami K., Harrison C., Kitamura K., Katayama S., Dhut S., Hunt T., Toda T.
      J. Biol. Chem. 279:18974-18980(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH POP1.
    11. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRUM1_SCHPO
    AccessioniPrimary (citable) accession number: P40380
    Secondary accession number(s): O74373
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: January 11, 2001
    Last modified: October 1, 2014
    This is version 87 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names

    External Data

    Dasty 3