P40380 (RUM1_SCHPO) Reviewed, UniProtKB/Swiss-Prot
Last modified June 11, 2014. Version 86. History...
Names and origin
|Protein names||Recommended name:|
Cyclin-dependent kinase inhibitor rum1
|Organism||Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]|
|Taxonomic identifier||284812 [NCBI]|
|Taxonomic lineage||Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces ›|
|Sequence length||230 AA.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
Regulator of cell cycle G1 phase progression. Ensures the correct sequence of S phase and mitosis in the cell by acting as an inhibitor of the cdc2 mitotic kinase. Probably interacts with cdc2 to inhibit its action until the cell mass for Start is reached. Determines the length of the pre-Start G1 period and prevents mitosis from happening in early G1 cells. Required for maintaining pheromone-induced G1 arrest. Acts as an adapter protein since interaction with cdc13 promotes cyclin proteolysis during G1. Becomes a target for degradation at the G1/S phase transition, following phosphorylation by cig1-associated cdc2 at the G1/S phase transition. Ref.1 Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9
Expression increases in nitrogen deprived environment, which is important to cause delay of the G1 phase of the cell cycle in response to nitrogen starvation. Ref.9
|Biological process||Cell cycle|
|Technical term||Complete proteome|
|Gene Ontology (GO)|
|Biological_process||G1 cell size control checkpointcell cycle arrest in response to nitrogen starvationcell cycle arrest in response to pheromonecellular response to nitrogen starvationcyclin catabolic process|
Non-traceable author statement. Source: PomBasenegative regulation of cyclin-dependent protein serine/threonine kinase activitynegative regulation of protein phosphorylationregulation of DNA-dependent DNA replication initiationsignal transduction
|Molecular_function||cyclin-dependent protein serine/threonine kinase inhibitor activityprotein binding|
|Complete GO annotation...|
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 230||230||Cyclin-dependent kinase inhibitor rum1||PRO_0000097531|
|Region||67 – 147||81||CDK inhibitory and cyclin-binding|
|Region||101 – 230||130||Required for activity as a cdc2 kinase inhibitor|
|Compositional bias||151 – 154||4||Poly-Ser|
|Compositional bias||192 – 197||6||Poly-Ser|
Amino acid modifications
|Modified residue||13||1||Phosphothreonine; by MAPK Ref.8|
|Modified residue||19||1||Phosphoserine; by MAPK Ref.8|
|Modified residue||58||1||Phosphothreonine; by cdc2 Ref.5|
|Modified residue||62||1||Phosphothreonine; by cdc2 Ref.5|
|Mutagenesis||5||1||T → A: No effect on phenotype and no reduction in 32-P incorporation mediated by MAPK. Ref.5 Ref.8|
|Mutagenesis||13||1||T → A: No effect on phenotype; when associated with A-16 and A-19. Reduced 32-P incorporation mediated by MAPK; when associated with A-19. Ref.5 Ref.8|
|Mutagenesis||13||1||T → E: Reduces activity as a cdc2 inhibitor; when associated with E-19. Ref.5 Ref.8|
|Mutagenesis||16||1||T → A: No effect on phenotype; when associated with A-13 and A-19. No reduction in 32-P incorporation mediated by MAPK. Ref.5 Ref.8|
|Mutagenesis||19||1||S → A: No effect on phenotype; when associated with A-13 and A-16. Reduced 32-P incorporation mediated by MAPK; when associated with A-13. Ref.5 Ref.8|
|Mutagenesis||19||1||S → E: Reduces activity as a cdc2 inhibitor; when associated with E-13. Ref.5 Ref.8|
|Mutagenesis||58||1||T → A: Reduces growth rate into small colonies containing many elongated cells. Phenotype enhances due to increased stability; when associated with A-62. No reduction in 32-P incorporation mediated by MAPK. Ref.5 Ref.8|
|Mutagenesis||58||1||T → S: Shift in mobility; when associated with S-62. Ref.5 Ref.8|
|Mutagenesis||62||1||T → A: Reduces growth rate into small colonies containing many elongated cells. Phenotype enhances due to increased stability; when associated with A-58. Ref.5|
|Mutagenesis||62||1||T → S: Shift in mobility; when associated with S-58. Ref.5|
|Mutagenesis||110||1||T → A: No effect on phenotype. Ref.5|
|Mutagenesis||212||1||S → A: No effect on phenotype. Ref.5|
|Sequence conflict||72 – 73||2||ML → IV in CAA54786. Ref.1|
|||"Regulation of progression through the G1 phase of the cell cycle by the rum1+ gene."|
Moreno S., Nurse P.
Nature 367:236-242(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
Strain: 972 / ATCC 24843.
|||"The genome sequence of Schizosaccharomyces pombe."|
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
|||"p25rum1 orders S phase and mitosis by acting as an inhibitor of the p34cdc2 mitotic kinase."|
Correa-Bordes J., Nurse P.
Cell 83:1001-1009(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
|||"p25rum1 promotes proteolysis of the mitotic B-cyclin p56cdc13 during G1 of the fission yeast cell cycle."|
Correa-Bordes J., Gulli M.P., Nurse P.
EMBO J. 16:4657-4664(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CDC13, PHOSPHORYLATION.
|||"Regulation of the G1 phase of the cell cycle by periodic stabilization and degradation of the p25rum1 CDK inhibitor."|
Benito J., Martin-Castellanos C., Moreno S.
EMBO J. 17:482-497(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PHOSPHORYLATION AT THR-58 AND THR-62, MUTAGENESIS OF THR-5; THR-13; THR-16; SER-19; THR-58; THR-62; THR-110 AND SER-212.
|||"The Cdk inhibitors p25rum1 and p40SIC1 are functional homologues that play similar roles in the regulation of the cell cycle in fission and budding yeast."|
Sanchez-Diaz A., Gonzalez I., Arellano M., Moreno S.
J. Cell Sci. 111:843-851(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CDC13, DOMAIN.
|||"Cyclin B proteolysis and the cyclin-dependent kinase inhibitor rum1p are required for pheromone-induced G1 arrest in fission yeast."|
Stern B., Nurse P.
Mol. Biol. Cell 9:1309-1321(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH CDC13 AND CIG2.
|||"Rum1, an inhibitor of cyclin-dependent kinase in fission yeast, is negatively regulated by mitogen-activated protein kinase-mediated phosphorylation at Ser and Thr residues."|
Matsuoka K., Kiyokawa N., Taguchi T., Matsui J., Suzuki T., Mimori K., Nakajima H., Takenouchi H., Weiran T., Katagiri Y.U., Fujimoto J.
Eur. J. Biochem. 269:3511-3521(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PHOSPHORYLATION AT THR-13 AND SER-19, MUTAGENESIS OF THR-5; THR-13; THR-16; SER-19 AND THR-58.
|||"Regulated mRNA stability of the Cdk inhibitor Rum1 links nutrient status to cell cycle progression."|
Daga R.R., Bolanos P., Moreno S.
Curr. Biol. 13:2015-2024(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION.
|||"Requirement of the SCFPop1/Pop2 ubiquitin ligase for degradation of the fission yeast S phase cyclin Cig2."|
Yamano H., Kominami K., Harrison C., Kitamura K., Katayama S., Dhut S., Hunt T., Toda T.
J. Biol. Chem. 279:18974-18980(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH POP1.
|||"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."|
Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
|+||Additional computationally mapped references.|
|X77730 mRNA. Translation: CAA54786.1.|
CU329671 Genomic DNA. Translation: CAA19370.1.
|RefSeq||NP_596152.1. NM_001022071.2. |
3D structure databases
Protein-protein interaction databases
|BioGrid||276195. 31 interactions.|
|IntAct||P40380. 5 interactions.|
Protocols and materials databases
Genome annotation databases
|EnsemblFungi||SPBC32F12.09.1; SPBC32F12.09.1:pep; SPBC32F12.09. |
Family and domain databases
|Accession||Primary (citable) accession number: P40380|
Secondary accession number(s): O74373
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Fungal Protein Annotation Program|
Schizosaccharomyces pombe: entries and gene names