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P40380

- RUM1_SCHPO

UniProt

P40380 - RUM1_SCHPO

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Protein

Cyclin-dependent kinase inhibitor rum1

Gene
rum1, SPBC32F12.09
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Regulator of cell cycle G1 phase progression. Ensures the correct sequence of S phase and mitosis in the cell by acting as an inhibitor of the cdc2 mitotic kinase. Probably interacts with cdc2 to inhibit its action until the cell mass for Start is reached. Determines the length of the pre-Start G1 period and prevents mitosis from happening in early G1 cells. Required for maintaining pheromone-induced G1 arrest. Acts as an adapter protein since interaction with cdc13 promotes cyclin proteolysis during G1. Becomes a target for degradation at the G1/S phase transition, following phosphorylation by cig1-associated cdc2 at the G1/S phase transition.8 Publications

GO - Molecular functioni

  1. cyclin-dependent protein serine/threonine kinase inhibitor activity Source: PomBase
  2. protein binding Source: IntAct

GO - Biological processi

  1. cell cycle arrest in response to nitrogen starvation Source: PomBase
  2. cell cycle arrest in response to pheromone Source: PomBase
  3. cellular response to nitrogen starvation Source: PomBase
  4. cyclin catabolic process Source: PomBase
  5. G1 cell size control checkpoint Source: PomBase
  6. negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: PomBase
  7. negative regulation of protein phosphorylation Source: PomBase
  8. regulation of DNA-dependent DNA replication initiation Source: PomBase
  9. signal transduction Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Cell cycle

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase inhibitor rum1
Alternative name(s):
p25-rum1
Gene namesi
Name:rum1
ORF Names:SPBC32F12.09
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome II

Organism-specific databases

PomBaseiSPBC32F12.09.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi5 – 51T → A: No effect on phenotype and no reduction in 32-P incorporation mediated by MAPK. 2 Publications
Mutagenesisi13 – 131T → A: No effect on phenotype; when associated with A-16 and A-19. Reduced 32-P incorporation mediated by MAPK; when associated with A-19. 2 Publications
Mutagenesisi13 – 131T → E: Reduces activity as a cdc2 inhibitor; when associated with E-19. 2 Publications
Mutagenesisi16 – 161T → A: No effect on phenotype; when associated with A-13 and A-19. No reduction in 32-P incorporation mediated by MAPK. 2 Publications
Mutagenesisi19 – 191S → A: No effect on phenotype; when associated with A-13 and A-16. Reduced 32-P incorporation mediated by MAPK; when associated with A-13. 2 Publications
Mutagenesisi19 – 191S → E: Reduces activity as a cdc2 inhibitor; when associated with E-13. 2 Publications
Mutagenesisi58 – 581T → A: Reduces growth rate into small colonies containing many elongated cells. Phenotype enhances due to increased stability; when associated with A-62. No reduction in 32-P incorporation mediated by MAPK. 2 Publications
Mutagenesisi58 – 581T → S: Shift in mobility; when associated with S-62. 2 Publications
Mutagenesisi62 – 621T → A: Reduces growth rate into small colonies containing many elongated cells. Phenotype enhances due to increased stability; when associated with A-58. 1 Publication
Mutagenesisi62 – 621T → S: Shift in mobility; when associated with S-58. 1 Publication
Mutagenesisi110 – 1101T → A: No effect on phenotype. 1 Publication
Mutagenesisi212 – 2121S → A: No effect on phenotype. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 230230Cyclin-dependent kinase inhibitor rum1PRO_0000097531Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei13 – 131Phosphothreonine; by MAPK1 Publication
Modified residuei19 – 191Phosphoserine; by MAPK1 Publication
Modified residuei58 – 581Phosphothreonine; by cdc21 Publication
Modified residuei62 – 621Phosphothreonine; by cdc21 Publication

Post-translational modificationi

Phosphorylated by cig1-associated cdc2 which leads to increased stability. Phosphorylation by MAPK reduces cdc2 kinase inhibitor ability.3 Publications

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

Expression increases in nitrogen deprived environment, which is important to cause delay of the G1 phase of the cell cycle in response to nitrogen starvation.1 Publication

Interactioni

Subunit structurei

Interacts with cdc13, cig2 and pop1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
cdc13P108155EBI-1187892,EBI-1187843
cdc2P045512EBI-1187892,EBI-1187862
cig2P366302EBI-1187892,EBI-1149212

Protein-protein interaction databases

BioGridi276195. 31 interactions.
IntActiP40380. 5 interactions.
MINTiMINT-4689673.
STRINGi4896.SPBC32F12.09-1.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni67 – 14781CDK inhibitory and cyclin-bindingAdd
BLAST
Regioni101 – 230130Required for activity as a cdc2 kinase inhibitorAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi151 – 1544Poly-Ser
Compositional biasi192 – 1976Poly-Ser

Phylogenomic databases

OMAiDICIFRR.

Sequencei

Sequence statusi: Complete.

P40380-1 [UniParc]FASTAAdd to Basket

« Hide

MEPSTPPMRG LCTPSTPESP GSFKGVIDAS LEGNSSIMID EIPESDLPAP    50
QVSTFPPTPA KTPKKQLLPN LMLQDRSNSL ERCMEEDREH NPFLSSSDNQ 100
LLSRKKRKPT PPPSDGLYYV FRGKRIKKSF RPGTDLSTFK PKLLFADSAP 150
SSSSDNPTSS VDLNDYSQIG ILPPNLNSIG NKMFSLKSRV PSSSSGSFVA 200
PPPQMRLPAY SSPQKSRSNT KDENRHNLLR 230
Length:230
Mass (Da):25,289
Last modified:January 11, 2001 - v2
Checksum:iC5199FE345F7484A
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti72 – 732ML → IV in CAA54786. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X77730 mRNA. Translation: CAA54786.1.
CU329671 Genomic DNA. Translation: CAA19370.1.
PIRiS41043.
T40233.
RefSeqiNP_596152.1. NM_001022071.2.

Genome annotation databases

EnsemblFungiiSPBC32F12.09.1; SPBC32F12.09.1:pep; SPBC32F12.09.
GeneIDi2539640.
KEGGispo:SPBC32F12.09.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X77730 mRNA. Translation: CAA54786.1 .
CU329671 Genomic DNA. Translation: CAA19370.1 .
PIRi S41043.
T40233.
RefSeqi NP_596152.1. NM_001022071.2.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 276195. 31 interactions.
IntActi P40380. 5 interactions.
MINTi MINT-4689673.
STRINGi 4896.SPBC32F12.09-1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPBC32F12.09.1 ; SPBC32F12.09.1:pep ; SPBC32F12.09 .
GeneIDi 2539640.
KEGGi spo:SPBC32F12.09.

Organism-specific databases

PomBasei SPBC32F12.09.

Phylogenomic databases

OMAi DICIFRR.

Miscellaneous databases

NextBioi 20800794.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Regulation of progression through the G1 phase of the cell cycle by the rum1+ gene."
    Moreno S., Nurse P.
    Nature 367:236-242(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Strain: 972 / ATCC 24843.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "p25rum1 orders S phase and mitosis by acting as an inhibitor of the p34cdc2 mitotic kinase."
    Correa-Bordes J., Nurse P.
    Cell 83:1001-1009(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "p25rum1 promotes proteolysis of the mitotic B-cyclin p56cdc13 during G1 of the fission yeast cell cycle."
    Correa-Bordes J., Gulli M.P., Nurse P.
    EMBO J. 16:4657-4664(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CDC13, PHOSPHORYLATION.
  5. "Regulation of the G1 phase of the cell cycle by periodic stabilization and degradation of the p25rum1 CDK inhibitor."
    Benito J., Martin-Castellanos C., Moreno S.
    EMBO J. 17:482-497(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION AT THR-58 AND THR-62, MUTAGENESIS OF THR-5; THR-13; THR-16; SER-19; THR-58; THR-62; THR-110 AND SER-212.
  6. "The Cdk inhibitors p25rum1 and p40SIC1 are functional homologues that play similar roles in the regulation of the cell cycle in fission and budding yeast."
    Sanchez-Diaz A., Gonzalez I., Arellano M., Moreno S.
    J. Cell Sci. 111:843-851(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CDC13, DOMAIN.
  7. "Cyclin B proteolysis and the cyclin-dependent kinase inhibitor rum1p are required for pheromone-induced G1 arrest in fission yeast."
    Stern B., Nurse P.
    Mol. Biol. Cell 9:1309-1321(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH CDC13 AND CIG2.
  8. "Rum1, an inhibitor of cyclin-dependent kinase in fission yeast, is negatively regulated by mitogen-activated protein kinase-mediated phosphorylation at Ser and Thr residues."
    Matsuoka K., Kiyokawa N., Taguchi T., Matsui J., Suzuki T., Mimori K., Nakajima H., Takenouchi H., Weiran T., Katagiri Y.U., Fujimoto J.
    Eur. J. Biochem. 269:3511-3521(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION AT THR-13 AND SER-19, MUTAGENESIS OF THR-5; THR-13; THR-16; SER-19 AND THR-58.
  9. "Regulated mRNA stability of the Cdk inhibitor Rum1 links nutrient status to cell cycle progression."
    Daga R.R., Bolanos P., Moreno S.
    Curr. Biol. 13:2015-2024(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  10. "Requirement of the SCFPop1/Pop2 ubiquitin ligase for degradation of the fission yeast S phase cyclin Cig2."
    Yamano H., Kominami K., Harrison C., Kitamura K., Katayama S., Dhut S., Hunt T., Toda T.
    J. Biol. Chem. 279:18974-18980(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH POP1.
  11. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRUM1_SCHPO
AccessioniPrimary (citable) accession number: P40380
Secondary accession number(s): O74373
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 11, 2001
Last modified: June 11, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names

External Data

Dasty 3