##gff-version 3 P40380 UniProtKB Chain 1 230 . . . ID=PRO_0000097531;Note=Cyclin-dependent kinase inhibitor rum1 P40380 UniProtKB Region 1 25 . . . Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite P40380 UniProtKB Region 43 118 . . . Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite P40380 UniProtKB Region 67 147 . . . Note=CDK inhibitory and cyclin-binding P40380 UniProtKB Region 101 230 . . . Note=Required for activity as a cdc2 kinase inhibitor P40380 UniProtKB Region 187 230 . . . Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite P40380 UniProtKB Compositional bias 79 93 . . . Note=Basic and acidic residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite P40380 UniProtKB Modified residue 13 13 . . . Note=Phosphothreonine%3B by MAPK;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12135491;Dbxref=PMID:12135491 P40380 UniProtKB Modified residue 19 19 . . . Note=Phosphoserine%3B by MAPK;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:12135491;Dbxref=PMID:12135491 P40380 UniProtKB Modified residue 58 58 . . . Note=Phosphothreonine%3B by cdc2;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9430640;Dbxref=PMID:9430640 P40380 UniProtKB Modified residue 62 62 . . . Note=Phosphothreonine%3B by cdc2;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9430640;Dbxref=PMID:9430640 P40380 UniProtKB Mutagenesis 5 5 . . . Note=No effect on phenotype and no reduction in 32-P incorporation mediated by MAPK. T->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:12135491,ECO:0000269|PubMed:9430640;Dbxref=PMID:12135491,PMID:9430640 P40380 UniProtKB Mutagenesis 13 13 . . . Note=No effect on phenotype%3B when associated with A-16 and A-19. Reduced 32-P incorporation mediated by MAPK%3B when associated with A-19. T->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:12135491,ECO:0000269|PubMed:9430640;Dbxref=PMID:12135491,PMID:9430640 P40380 UniProtKB Mutagenesis 13 13 . . . Note=Reduces activity as a cdc2 inhibitor%3B when associated with E-19. T->E;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:12135491,ECO:0000269|PubMed:9430640;Dbxref=PMID:12135491,PMID:9430640 P40380 UniProtKB Mutagenesis 16 16 . . . Note=No effect on phenotype%3B when associated with A-13 and A-19. No reduction in 32-P incorporation mediated by MAPK. T->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:12135491,ECO:0000269|PubMed:9430640;Dbxref=PMID:12135491,PMID:9430640 P40380 UniProtKB Mutagenesis 19 19 . . . Note=No effect on phenotype%3B when associated with A-13 and A-16. Reduced 32-P incorporation mediated by MAPK%3B when associated with A-13. S->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:12135491,ECO:0000269|PubMed:9430640;Dbxref=PMID:12135491,PMID:9430640 P40380 UniProtKB Mutagenesis 19 19 . . . Note=Reduces activity as a cdc2 inhibitor%3B when associated with E-13. S->E;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:12135491,ECO:0000269|PubMed:9430640;Dbxref=PMID:12135491,PMID:9430640 P40380 UniProtKB Mutagenesis 58 58 . . . Note=Reduces growth rate into small colonies containing many elongated cells. Phenotype enhances due to increased stability%3B when associated with A-62. No reduction in 32-P incorporation mediated by MAPK. T->A;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:12135491,ECO:0000269|PubMed:9430640;Dbxref=PMID:12135491,PMID:9430640 P40380 UniProtKB Mutagenesis 58 58 . . . Note=Shift in mobility%3B when associated with S-62. T->S;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:12135491,ECO:0000269|PubMed:9430640;Dbxref=PMID:12135491,PMID:9430640 P40380 UniProtKB Mutagenesis 62 62 . . . Note=Reduces growth rate into small colonies containing many elongated cells. Phenotype enhances due to increased stability%3B when associated with A-58. T->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9430640;Dbxref=PMID:9430640 P40380 UniProtKB Mutagenesis 62 62 . . . Note=Shift in mobility%3B when associated with S-58. T->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9430640;Dbxref=PMID:9430640 P40380 UniProtKB Mutagenesis 110 110 . . . Note=No effect on phenotype. T->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9430640;Dbxref=PMID:9430640 P40380 UniProtKB Mutagenesis 212 212 . . . Note=No effect on phenotype. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9430640;Dbxref=PMID:9430640 P40380 UniProtKB Sequence conflict 72 73 . . . Note=ML->IV;Ontology_term=ECO:0000305;evidence=ECO:0000305