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Protein

Cyclin-dependent kinase inhibitor rum1

Gene

rum1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulator of cell cycle G1 phase progression. Ensures the correct sequence of S phase and mitosis in the cell by acting as an inhibitor of the cdc2 mitotic kinase. Probably interacts with cdc2 to inhibit its action until the cell mass for Start is reached. Determines the length of the pre-Start G1 period and prevents mitosis from happening in early G1 cells. Required for maintaining pheromone-induced G1 arrest. Acts as an adapter protein since interaction with cdc13 promotes cyclin proteolysis during G1. Becomes a target for degradation at the G1/S phase transition, following phosphorylation by cig1-associated cdc2 at the G1/S phase transition.8 Publications

GO - Molecular functioni

GO - Biological processi

  • G1 cell size control checkpoint Source: PomBase
  • mitotic cell cycle arrest in response to pheromone Source: PomBase
  • mitotic G1 cell cycle arrest in response to nitrogen starvation Source: PomBase
  • negative regulation of mitotic DNA replication initiation Source: PomBase
  • negative regulation of protein phosphorylation Source: PomBase
Complete GO annotation...

Keywords - Biological processi

Cell cycle

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase inhibitor rum1
Alternative name(s):
p25-rum1
Gene namesi
Name:rum1
ORF Names:SPBC32F12.09
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC32F12.09.
PomBaseiSPBC32F12.09. rum1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi5T → A: No effect on phenotype and no reduction in 32-P incorporation mediated by MAPK. 2 Publications1
Mutagenesisi13T → A: No effect on phenotype; when associated with A-16 and A-19. Reduced 32-P incorporation mediated by MAPK; when associated with A-19. 2 Publications1
Mutagenesisi13T → E: Reduces activity as a cdc2 inhibitor; when associated with E-19. 2 Publications1
Mutagenesisi16T → A: No effect on phenotype; when associated with A-13 and A-19. No reduction in 32-P incorporation mediated by MAPK. 2 Publications1
Mutagenesisi19S → A: No effect on phenotype; when associated with A-13 and A-16. Reduced 32-P incorporation mediated by MAPK; when associated with A-13. 2 Publications1
Mutagenesisi19S → E: Reduces activity as a cdc2 inhibitor; when associated with E-13. 2 Publications1
Mutagenesisi58T → A: Reduces growth rate into small colonies containing many elongated cells. Phenotype enhances due to increased stability; when associated with A-62. No reduction in 32-P incorporation mediated by MAPK. 2 Publications1
Mutagenesisi58T → S: Shift in mobility; when associated with S-62. 2 Publications1
Mutagenesisi62T → A: Reduces growth rate into small colonies containing many elongated cells. Phenotype enhances due to increased stability; when associated with A-58. 1 Publication1
Mutagenesisi62T → S: Shift in mobility; when associated with S-58. 1 Publication1
Mutagenesisi110T → A: No effect on phenotype. 1 Publication1
Mutagenesisi212S → A: No effect on phenotype. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000975311 – 230Cyclin-dependent kinase inhibitor rum1Add BLAST230

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei13Phosphothreonine; by MAPK1 Publication1
Modified residuei19Phosphoserine; by MAPK1 Publication1
Modified residuei58Phosphothreonine; by cdc21 Publication1
Modified residuei62Phosphothreonine; by cdc21 Publication1

Post-translational modificationi

Phosphorylated by cig1-associated cdc2 which leads to increased stability. Phosphorylation by MAPK reduces cdc2 kinase inhibitor ability.3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP40380.

PTM databases

iPTMnetiP40380.

Expressioni

Inductioni

Expression increases in nitrogen deprived environment, which is important to cause delay of the G1 phase of the cell cycle in response to nitrogen starvation.1 Publication

Interactioni

Subunit structurei

Interacts with cdc13, cig2 and pop1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
cdc13P108155EBI-1187892,EBI-1187843
cdc2P045512EBI-1187892,EBI-1187862
cig2P366302EBI-1187892,EBI-1149212

Protein-protein interaction databases

BioGridi276195. 31 interactors.
IntActiP40380. 5 interactors.
MINTiMINT-4689673.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni67 – 147CDK inhibitory and cyclin-bindingAdd BLAST81
Regioni101 – 230Required for activity as a cdc2 kinase inhibitorAdd BLAST130

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi151 – 154Poly-Ser4
Compositional biasi192 – 197Poly-Ser6

Phylogenomic databases

OrthoDBiEOG092C57HR.

Sequencei

Sequence statusi: Complete.

P40380-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPSTPPMRG LCTPSTPESP GSFKGVIDAS LEGNSSIMID EIPESDLPAP
60 70 80 90 100
QVSTFPPTPA KTPKKQLLPN LMLQDRSNSL ERCMEEDREH NPFLSSSDNQ
110 120 130 140 150
LLSRKKRKPT PPPSDGLYYV FRGKRIKKSF RPGTDLSTFK PKLLFADSAP
160 170 180 190 200
SSSSDNPTSS VDLNDYSQIG ILPPNLNSIG NKMFSLKSRV PSSSSGSFVA
210 220 230
PPPQMRLPAY SSPQKSRSNT KDENRHNLLR
Length:230
Mass (Da):25,289
Last modified:January 11, 2001 - v2
Checksum:iC5199FE345F7484A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti72 – 73ML → IV in CAA54786 (PubMed:8121488).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77730 mRNA. Translation: CAA54786.1.
CU329671 Genomic DNA. Translation: CAA19370.1.
PIRiS41043.
T40233.
RefSeqiNP_596152.1. NM_001022071.2.

Genome annotation databases

EnsemblFungiiSPBC32F12.09.1; SPBC32F12.09.1:pep; SPBC32F12.09.
GeneIDi2539640.
KEGGispo:SPBC32F12.09.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77730 mRNA. Translation: CAA54786.1.
CU329671 Genomic DNA. Translation: CAA19370.1.
PIRiS41043.
T40233.
RefSeqiNP_596152.1. NM_001022071.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276195. 31 interactors.
IntActiP40380. 5 interactors.
MINTiMINT-4689673.

PTM databases

iPTMnetiP40380.

Proteomic databases

PRIDEiP40380.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC32F12.09.1; SPBC32F12.09.1:pep; SPBC32F12.09.
GeneIDi2539640.
KEGGispo:SPBC32F12.09.

Organism-specific databases

EuPathDBiFungiDB:SPBC32F12.09.
PomBaseiSPBC32F12.09. rum1.

Phylogenomic databases

OrthoDBiEOG092C57HR.

Miscellaneous databases

PROiP40380.

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiRUM1_SCHPO
AccessioniPrimary (citable) accession number: P40380
Secondary accession number(s): O74373
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 11, 2001
Last modified: October 5, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.