P40380 (RUM1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase inhibitor rum1

Alternative name(s):
p25-rum1

Gene names

Name:	rum1
ORF Names:	SPBC32F12.09

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulator of cell cycle G1 phase progression. Ensures the correct sequence of S phase and mitosis in the cell by acting as an inhibitor of the cdc2 mitotic kinase. Probably interacts with cdc2 to inhibit its action until the cell mass for Start is reached. Determines the length of the pre-Start G1 period and prevents mitosis from happening in early G1 cells. Required for maintaining pheromone-induced G1 arrest. Acts as an adapter protein since interaction with cdc13 promotes cyclin proteolysis during G1. Becomes a target for degradation at the G1/S phase transition, following phosphorylation by cig1-associated cdc2 at the G1/S phase transition. Ref.1 Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 Ref.9
Subunit structure	Interacts with cdc13, cig2 and pop1. Ref.4 Ref.6 Ref.7 Ref.10
Subcellular location	Nucleus Ref.11.
Induction	Expression increases in nitrogen deprived environment, which is important to cause delay of the G1 phase of the cell cycle in response to nitrogen starvation. Ref.9
Post-translational modification	Phosphorylated by cig1-associated cdc2 which leads to increased stability. Phosphorylation by MAPK reduces cdc2 kinase inhibitor ability. Ref.4 Ref.5 Ref.8

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Nucleus
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell cycle arrest in response to nitrogen starvation Inferred from mutant phenotype Ref.9. Source: PomBase cell cycle arrest in response to pheromone Inferred from mutant phenotype Ref.7. Source: PomBase cyclin catabolic process Non-traceable author statement. Source: PomBase negative regulation of cyclin-dependent protein serine/threonine kinase activity Traceable author statement Ref.3. Source: PomBase regulation of DNA-dependent DNA replication initiation Inferred from genetic interaction PubMed 8598285. Source: PomBase regulation of M/G1 transition of mitotic cell cycle Inferred from direct assay PubMed 9552380. Source: PomBase signal transduction Traceable author statement PubMed 15157496. Source: PomBase
Cellular_component	nucleus Inferred from direct assay Ref.11. Source: PomBase
Molecular_function	cyclin-dependent protein serine/threonine kinase inhibitor activity Traceable author statement PubMed 15157496. Source: PomBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
cdc13	P10815	5	EBI-1187892,EBI-1187843
cdc2	P04551	2	EBI-1187892,EBI-1187862
cig2	P36630	2	EBI-1187892,EBI-1149212

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 230	230	Cyclin-dependent kinase inhibitor rum1		PRO_0000097531
Regions
	Region	67 – 147	81	CDK inhibitory and cyclin-binding
	Region	101 – 230	130	Required for activity as a cdc2 kinase inhibitor
	Compositional bias	151 – 154	4	Poly-Ser
	Compositional bias	192 – 197	6	Poly-Ser
Amino acid modifications
	Modified residue	13	1	Phosphothreonine; by MAPK Ref.8
	Modified residue	19	1	Phosphoserine; by MAPK Ref.8
	Modified residue	58	1	Phosphothreonine; by cdc2 Ref.5
	Modified residue	62	1	Phosphothreonine; by cdc2 Ref.5
Experimental info
	Mutagenesis	5	1	T → A: No effect on phenotype and no reduction in 32-P incorporation mediated by MAPK. Ref.5 Ref.8
	Mutagenesis	13	1	T → A: No effect on phenotype; when associated with A-16 and A-19. Reduced 32-P incorporation mediated by MAPK; when associated with A-19. Ref.5 Ref.8
	Mutagenesis	13	1	T → E: Reduces activity as a cdc2 inhibitor; when associated with E-19. Ref.5 Ref.8
	Mutagenesis	16	1	T → A: No effect on phenotype; when associated with A-13 and A-19. No reduction in 32-P incorporation mediated by MAPK. Ref.5 Ref.8
	Mutagenesis	19	1	S → A: No effect on phenotype; when associated with A-13 and A-16. Reduced 32-P incorporation mediated by MAPK; when associated with A-13. Ref.5 Ref.8
	Mutagenesis	19	1	S → E: Reduces activity as a cdc2 inhibitor; when associated with E-13. Ref.5 Ref.8
	Mutagenesis	58	1	T → A: Reduces growth rate into small colonies containing many elongated cells. Phenotype enhances due to increased stability; when associated with A-62. No reduction in 32-P incorporation mediated by MAPK. Ref.5 Ref.8
	Mutagenesis	58	1	T → S: Shift in mobility; when associated with S-62. Ref.5 Ref.8
	Mutagenesis	62	1	T → A: Reduces growth rate into small colonies containing many elongated cells. Phenotype enhances due to increased stability; when associated with A-58. Ref.5
	Mutagenesis	62	1	T → S: Shift in mobility; when associated with S-58. Ref.5
	Mutagenesis	110	1	T → A: No effect on phenotype. Ref.5
	Mutagenesis	212	1	S → A: No effect on phenotype. Ref.5
	Sequence conflict	72 – 73	2	ML → IV in CAA54786. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P40380 [UniParc]. Last modified January 11, 2001. Version 2. Checksum: C5199FE345F7484A	FASTA	230	25,289
	10 20 30 40 50 60 MEPSTPPMRG LCTPSTPESP GSFKGVIDAS LEGNSSIMID EIPESDLPAP QVSTFPPTPA 70 80 90 100 110 120 KTPKKQLLPN LMLQDRSNSL ERCMEEDREH NPFLSSSDNQ LLSRKKRKPT PPPSDGLYYV 130 140 150 160 170 180 FRGKRIKKSF RPGTDLSTFK PKLLFADSAP SSSSDNPTSS VDLNDYSQIG ILPPNLNSIG 190 200 210 220 230 NKMFSLKSRV PSSSSGSFVA PPPQMRLPAY SSPQKSRSNT KDENRHNLLR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Regulation of progression through the G1 phase of the cell cycle by the rum1+ gene." Moreno S., Nurse P. Nature 367:236-242(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION. Strain: 972 / ATCC 24843.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[3]	"p25rum1 orders S phase and mitosis by acting as an inhibitor of the p34cdc2 mitotic kinase." Correa-Bordes J., Nurse P. Cell 83:1001-1009(1995) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[4]	"p25rum1 promotes proteolysis of the mitotic B-cyclin p56cdc13 during G1 of the fission yeast cell cycle." Correa-Bordes J., Gulli M.P., Nurse P. EMBO J. 16:4657-4664(1997) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CDC13, PHOSPHORYLATION.
[5]	"Regulation of the G1 phase of the cell cycle by periodic stabilization and degradation of the p25rum1 CDK inhibitor." Benito J., Martin-Castellanos C., Moreno S. EMBO J. 17:482-497(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION AT THR-58 AND THR-62, MUTAGENESIS OF THR-5; THR-13; THR-16; SER-19; THR-58; THR-62; THR-110 AND SER-212.
[6]	"The Cdk inhibitors p25rum1 and p40SIC1 are functional homologues that play similar roles in the regulation of the cell cycle in fission and budding yeast." Sanchez-Diaz A., Gonzalez I., Arellano M., Moreno S. J. Cell Sci. 111:843-851(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CDC13, DOMAIN.
[7]	"Cyclin B proteolysis and the cyclin-dependent kinase inhibitor rum1p are required for pheromone-induced G1 arrest in fission yeast." Stern B., Nurse P. Mol. Biol. Cell 9:1309-1321(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH CDC13 AND CIG2.
[8]	"Rum1, an inhibitor of cyclin-dependent kinase in fission yeast, is negatively regulated by mitogen-activated protein kinase-mediated phosphorylation at Ser and Thr residues." Matsuoka K., Kiyokawa N., Taguchi T., Matsui J., Suzuki T., Mimori K., Nakajima H., Takenouchi H., Weiran T., Katagiri Y.U., Fujimoto J. Eur. J. Biochem. 269:3511-3521(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, PHOSPHORYLATION AT THR-13 AND SER-19, MUTAGENESIS OF THR-5; THR-13; THR-16; SER-19 AND THR-58.
[9]	"Regulated mRNA stability of the Cdk inhibitor Rum1 links nutrient status to cell cycle progression." Daga R.R., Bolanos P., Moreno S. Curr. Biol. 13:2015-2024(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION.
[10]	"Requirement of the SCFPop1/Pop2 ubiquitin ligase for degradation of the fission yeast S phase cyclin Cig2." Yamano H., Kominami K., Harrison C., Kitamura K., Katayama S., Dhut S., Hunt T., Toda T. J. Biol. Chem. 279:18974-18980(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH POP1.
[11]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X77730 mRNA. Translation: CAA54786.1. CU329671 Genomic DNA. Translation: CAA19370.1.
PIR	S41043. T40233.
RefSeq	NP_596152.1. NM_001022071.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P40380. 5 interactions.
STRING	4896.SPBC32F12.09-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC32F12.09.1; SPBC32F12.09.1:pep; SPBC32F12.09.
GeneID	2539640.
KEGG	spo:SPBC32F12.09.
Organism-specific databases
PomBase	SPBC32F12.09.
Family and domain databases
ProtoNet	Search...
Other
NextBio	20800794.

Entry information

Entry name

RUM1_SCHPO

Accession

Primary (citable) accession number: P40380
Secondary accession number(s): O74373

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	January 11, 2001
Last modified:	May 1, 2013
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program