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Protein

DNA replication licensing factor mcm2

Gene

mcm2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as component of the mcm2-7 complex (mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity). Plays an important role in DNA replication.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri334 – 36027C4-typeSequence analysisAdd
BLAST
Nucleotide bindingi534 – 5418ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

  • mitotic DNA replication initiation Source: PomBase
  • negative regulation of helicase activity Source: PomBase
  • premeiotic DNA replication Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-SPO-68962. Activation of the pre-replicative complex.
R-SPO-69052. Switching of origins to a post-replicative state.
R-SPO-69300. Removal of licensing factors from origins.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor mcm2 (EC:3.6.4.12)
Alternative name(s):
Cell division control protein 19
Minichromosome maintenance protein 2
Gene namesi
Name:mcm2
Synonyms:cdc19, nda1
ORF Names:SPBC4.04c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC4.04c.
PomBaseiSPBC4.04c. mcm2.

Subcellular locationi

GO - Cellular componenti

  • DNA replication preinitiation complex Source: PomBase
  • MCM complex Source: PomBase
  • nuclear chromatin Source: PomBase
  • nuclear chromosome, telomeric region Source: PomBase
  • nuclear pre-replicative complex Source: PomBase
  • nuclear replication fork Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 830830DNA replication licensing factor mcm2PRO_0000194092Add
BLAST

Proteomic databases

MaxQBiP40377.

Interactioni

Subunit structurei

Component of the mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order mcm2-mcm6-mcm4-mcm7-mcm3-mcm5 (Probable). The heterodimers of mcm4/mcm6 and mcm3/mcm5 interact with mcm2 and mcm7. Interacts with mcm10.Curated1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
hsk1P505822EBI-783248,EBI-908476

Protein-protein interaction databases

BioGridi277146. 33 interactions.
IntActiP40377. 8 interactions.
MINTiMINT-4689642.

Structurei

3D structure databases

ProteinModelPortaliP40377.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini484 – 691208MCMAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi666 – 6694Arginine finger

Sequence similaritiesi

Belongs to the MCM family.Curated
Contains 1 MCM domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri334 – 36027C4-typeSequence analysisAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000224124.
InParanoidiP40377.
KOiK02540.
OMAiFSRYLSF.
OrthoDBiEOG092C0VUM.
PhylomeDBiP40377.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR031327. MCM.
IPR008045. MCM2.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11630:SF44. PTHR11630:SF44. 1 hit.
PfamiPF00493. MCM. 1 hit.
PF12619. MCM2_N. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01658. MCMPROTEIN2.
SMARTiSM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40377-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSFRKRGRR DSESLPFESE NSSLGATPLS LPPSSPPPEF SDEAAEALVE
60 70 80 90 100
EDIEDLDGEA LDVEDEEGED LFGEGMERDY QQNLELDRYD IEELDDDNDL
110 120 130 140 150
EELDIGARRA VDARLRRRDI ELDAAAGRTK PAAFLQDEDD DLDSNLGTGF
160 170 180 190 200
TRHRHRIYDE YSPNVGALDE SGELPLESIA DVKADSIAEW VTLDPVRRTI
210 220 230 240 250
AREFKNFLLE YTDENGTSVY GNRIRTLGEV NAESLMVNYA HLGESKPILA
260 270 280 290 300
YFLANAPAPI FRIFDRVALE ATLLHYPDYE RIHSDIHVRI TNLPTCFTLR
310 320 330 340 350
DLRQSHLNCL VRVSGVVTRR TGLFPQLKYI RFTCTKCGAT LGPFFQDSSV
360 370 380 390 400
EVKISFCHNC SSRGPFVINS ERTVYNNYQR ITLQESPGTV PSGRLPRHRE
410 420 430 440 450
VILLADLVDV AKPGEEIDVT GIYRNNFDAS LNTKNGFPVF ATIIEANHIS
460 470 480 490 500
QLDGSGNTDD DFSLSRLTDD EEREIRALAK SPDIHNRIIA SMAPSIYGHR
510 520 530 540 550
SIKTAIAAAL FGGVPKNING KHKIRGDINV LLLGDPGTAK SQFLKYVEKT
560 570 580 590 600
AHRAVFATGQ GASAVGLTAS VRKDPITNEW TLEGGALVLA DKGVCLIDEF
610 620 630 640 650
DKMNDQDRTS IHEAMEQQSI SISKAGIVTT LQARCTIIAA ANPIGGRYNT
660 670 680 690 700
TIPFNQNVEL TEPILSRFDI LQVVKDTVNP EIDEQLANFV VSSHIRSHPA
710 720 730 740 750
FDPNMDVLKK VPTETGIDAK PIPQDLLRKY IHFAREKVFP RLQQMDEEKI
760 770 780 790 800
SRLYSDMRRE SLATGSYPIT VRHLESAIRL SEAFAKMQLS EFVRPSHIDK
810 820 830
AIQVIIDSFV NAQKMSVKRS LSRTFAKYLI
Length:830
Mass (Da):92,831
Last modified:February 1, 1995 - v1
Checksum:iA5BF0AA29661BD66
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08048 Genomic DNA. Translation: AAC48930.1.
S68468 Genomic DNA. Translation: AAC60569.1.
CU329671 Genomic DNA. Translation: CAB58403.1.
PIRiB48723.
RefSeqiNP_595477.1. NM_001021388.2.

Genome annotation databases

EnsemblFungiiSPBC4.04c.1; SPBC4.04c.1:pep; SPBC4.04c.
GeneIDi2540620.
KEGGispo:SPBC4.04c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08048 Genomic DNA. Translation: AAC48930.1.
S68468 Genomic DNA. Translation: AAC60569.1.
CU329671 Genomic DNA. Translation: CAB58403.1.
PIRiB48723.
RefSeqiNP_595477.1. NM_001021388.2.

3D structure databases

ProteinModelPortaliP40377.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277146. 33 interactions.
IntActiP40377. 8 interactions.
MINTiMINT-4689642.

Proteomic databases

MaxQBiP40377.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC4.04c.1; SPBC4.04c.1:pep; SPBC4.04c.
GeneIDi2540620.
KEGGispo:SPBC4.04c.

Organism-specific databases

EuPathDBiFungiDB:SPBC4.04c.
PomBaseiSPBC4.04c. mcm2.

Phylogenomic databases

HOGENOMiHOG000224124.
InParanoidiP40377.
KOiK02540.
OMAiFSRYLSF.
OrthoDBiEOG092C0VUM.
PhylomeDBiP40377.

Enzyme and pathway databases

ReactomeiR-SPO-68962. Activation of the pre-replicative complex.
R-SPO-69052. Switching of origins to a post-replicative state.
R-SPO-69300. Removal of licensing factors from origins.

Miscellaneous databases

PROiP40377.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR031327. MCM.
IPR008045. MCM2.
IPR018525. MCM_CS.
IPR001208. MCM_dom.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11630:SF44. PTHR11630:SF44. 1 hit.
PfamiPF00493. MCM. 1 hit.
PF12619. MCM2_N. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01658. MCMPROTEIN2.
SMARTiSM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMCM2_SCHPO
AccessioniPrimary (citable) accession number: P40377
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 7, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.