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Protein

DNA replication licensing factor mcm2

Gene

mcm2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as component of the mcm2-7 complex (mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity). Plays an important role in DNA replication.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri334 – 360C4-typeSequence analysisAdd BLAST27
Nucleotide bindingi534 – 541ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • DNA duplex unwinding Source: GOC
  • mitotic DNA replication initiation Source: PomBase
  • negative regulation of DNA helicase activity Source: InterPro
  • negative regulation of helicase activity Source: PomBase
  • premeiotic DNA replication Source: PomBase

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processCell cycle, DNA replication
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-SPO-68962 Activation of the pre-replicative complex
R-SPO-69052 Switching of origins to a post-replicative state

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor mcm2 (EC:3.6.4.12)
Alternative name(s):
Cell division control protein 19
Minichromosome maintenance protein 2
Gene namesi
Name:mcm2
Synonyms:cdc19, nda1
ORF Names:SPBC4.04c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC4.04c
PomBaseiSPBC4.04c mcm2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001940921 – 830DNA replication licensing factor mcm2Add BLAST830

Proteomic databases

MaxQBiP40377
PaxDbiP40377
PRIDEiP40377

PTM databases

iPTMnetiP40377

Interactioni

Subunit structurei

Component of the mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order mcm2-mcm6-mcm4-mcm7-mcm3-mcm5 (Probable). The heterodimers of mcm4/mcm6 and mcm3/mcm5 interact with mcm2 and mcm7. Interacts with mcm10.Curated1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
hsk1P505822EBI-783248,EBI-908476

Protein-protein interaction databases

BioGridi277146, 33 interactors
IntActiP40377, 8 interactors
MINTiP40377
STRINGi4896.SPBC4.04c.1

Structurei

3D structure databases

ProteinModelPortaliP40377
SMRiP40377
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini484 – 691MCMAdd BLAST208

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi666 – 669Arginine finger4

Sequence similaritiesi

Belongs to the MCM family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri334 – 360C4-typeSequence analysisAdd BLAST27

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000224124
InParanoidiP40377
KOiK02540
OMAiKYDCVKC
OrthoDBiEOG092C0VUM
PhylomeDBiP40377

Family and domain databases

InterProiView protein in InterPro
IPR031327 MCM
IPR008045 MCM2
IPR018525 MCM_CS
IPR001208 MCM_dom
IPR027925 MCM_N
IPR033762 MCM_OB
IPR012340 NA-bd_OB-fold
IPR027417 P-loop_NTPase
PANTHERiPTHR11630 PTHR11630, 1 hit
PTHR11630:SF44 PTHR11630:SF44, 1 hit
PfamiView protein in Pfam
PF00493 MCM, 1 hit
PF12619 MCM2_N, 1 hit
PF14551 MCM_N, 1 hit
PF17207 MCM_OB, 1 hit
PRINTSiPR01657 MCMFAMILY
PR01658 MCMPROTEIN2
SMARTiView protein in SMART
SM00350 MCM, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00847 MCM_1, 1 hit
PS50051 MCM_2, 1 hit

Sequencei

Sequence statusi: Complete.

P40377-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDSFRKRGRR DSESLPFESE NSSLGATPLS LPPSSPPPEF SDEAAEALVE
60 70 80 90 100
EDIEDLDGEA LDVEDEEGED LFGEGMERDY QQNLELDRYD IEELDDDNDL
110 120 130 140 150
EELDIGARRA VDARLRRRDI ELDAAAGRTK PAAFLQDEDD DLDSNLGTGF
160 170 180 190 200
TRHRHRIYDE YSPNVGALDE SGELPLESIA DVKADSIAEW VTLDPVRRTI
210 220 230 240 250
AREFKNFLLE YTDENGTSVY GNRIRTLGEV NAESLMVNYA HLGESKPILA
260 270 280 290 300
YFLANAPAPI FRIFDRVALE ATLLHYPDYE RIHSDIHVRI TNLPTCFTLR
310 320 330 340 350
DLRQSHLNCL VRVSGVVTRR TGLFPQLKYI RFTCTKCGAT LGPFFQDSSV
360 370 380 390 400
EVKISFCHNC SSRGPFVINS ERTVYNNYQR ITLQESPGTV PSGRLPRHRE
410 420 430 440 450
VILLADLVDV AKPGEEIDVT GIYRNNFDAS LNTKNGFPVF ATIIEANHIS
460 470 480 490 500
QLDGSGNTDD DFSLSRLTDD EEREIRALAK SPDIHNRIIA SMAPSIYGHR
510 520 530 540 550
SIKTAIAAAL FGGVPKNING KHKIRGDINV LLLGDPGTAK SQFLKYVEKT
560 570 580 590 600
AHRAVFATGQ GASAVGLTAS VRKDPITNEW TLEGGALVLA DKGVCLIDEF
610 620 630 640 650
DKMNDQDRTS IHEAMEQQSI SISKAGIVTT LQARCTIIAA ANPIGGRYNT
660 670 680 690 700
TIPFNQNVEL TEPILSRFDI LQVVKDTVNP EIDEQLANFV VSSHIRSHPA
710 720 730 740 750
FDPNMDVLKK VPTETGIDAK PIPQDLLRKY IHFAREKVFP RLQQMDEEKI
760 770 780 790 800
SRLYSDMRRE SLATGSYPIT VRHLESAIRL SEAFAKMQLS EFVRPSHIDK
810 820 830
AIQVIIDSFV NAQKMSVKRS LSRTFAKYLI
Length:830
Mass (Da):92,831
Last modified:February 1, 1995 - v1
Checksum:iA5BF0AA29661BD66
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08048 Genomic DNA Translation: AAC48930.1
S68468 Genomic DNA Translation: AAC60569.1
CU329671 Genomic DNA Translation: CAB58403.1
PIRiB48723
RefSeqiNP_595477.1, NM_001021388.2

Genome annotation databases

EnsemblFungiiSPBC4.04c.1; SPBC4.04c.1:pep; SPBC4.04c
GeneIDi2540620
KEGGispo:SPBC4.04c

Similar proteinsi

Entry informationi

Entry nameiMCM2_SCHPO
AccessioniPrimary (citable) accession number: P40377
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 23, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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