Reviewed, UniProtKB/Swiss-Prot P40376 (KAPB_SCHPO)

Names and origin

Protein names

Recommended name:
    cAMP-dependent protein kinase catalytic subunit
    EC=2.7.11.11

Gene names

Name:	pka1
Synonyms:	tpk
ORF Names:	SPBC106.10

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Activated by cAMP By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding cAMP
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	age-dependent response to reactive oxygen species Inferred from genetic interaction. Source: GeneDB_SPombe cAMP-mediated signaling Inferred by curator. Source: GeneDB_SPombe chronological cell aging Inferred from genetic interaction. Source: GeneDB_SPombe induction of conjugation upon nitrogen starvation Inferred from mutant phenotype. Source: GeneDB_SPombe negative regulation of conjugation with cellular fusion Ref.2 Inferred from mutant phenotype. Source: GeneDB_SPombe negative regulation of transcription by glucose Inferred from mutant phenotype. Source: GeneDB_SPombe protein amino acid phosphorylation Inferred by curator. Source: GeneDB_SPombe
Cellular component	cAMP-dependent protein kinase complex Ref.2 Traceable author statement. Source: GeneDB_SPombe cytosol Inferred from direct assay. Source: GeneDB_SPombe nucleus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cAMP-dependent protein kinase activity Ref.2 Inferred from genetic interaction. Source: GeneDB_SPombe protein binding Inferred from physical interaction. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 512	512	cAMP-dependent protein kinase catalytic subunit		PRO_0000086047
Regions
	Domain	201 – 456	256	Protein kinase
	Domain	457 – 512	56	AGC-kinase C-terminal
	Nucleotide binding	207 – 215	9	ATP By similarity
Sites
	Active site	324	1	Proton acceptor By similarity
	Binding site	230	1	ATP By similarity
Amino acid modifications
	Modified residue	356	1	Phosphothreonine Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P40376-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: AF3547557AADDD84	FASTA	512	57,578
	10 20 30 40 50 60 MDTTAVASKG STNVGSSTDT LSTSASLHPS MNAGSVNEYS EQQRHGTNSF NGKPSVHDSV 70 80 90 100 110 120 GSDASVSNGH NNHNESSLWT SGIPKALEEA TKSKKPDSLV STSTSGCASA HSVGYQNIDN 130 140 150 160 170 180 LIPSPLPESA SRSSSQSSHQ RHSRDGRGEL GSEHGERRSA MDGLRDRHIR KVRVSQLLDL 190 200 210 220 230 240 QRRRIRPADH TTKDRYGIQD FNFLQTLGTG SFGRVHLVQS NHNRLYYAIK VLEKKKIVDM 250 260 270 280 290 300 KQIEHTCDER YILSRVQHPF ITILWGTFQD AKNLFMVMDF AEGGELFSLL RKCHRFPEKV 310 320 330 340 350 360 AKFYAAEVIL ALDYLHHNQI VYRDLKPENL LLDRFGHLKI VDFGFAKRVS TSNCCTLCGT 370 380 390 400 410 420 PDYLAPEIIS LKPYNKAADW WSLGILIFEM LAGYPPFYSE NPMKLYENIL EGKVNYPSYF 430 440 450 460 470 480 SPASIDLLSH LLQRDITCRY GNLKDGSMDI IMHPWFRDIS WDKILTRKIE VPYVPPIQAG 490 500 510 MGDSSQFDAY ADVATDYGTS EDPEFTSIFK DF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Schizosaccharomyces pombe pka1 gene, encoding a homolog of cAMP-dependent protein kinase." Yu G., Li J., Young D. Gene 151:215-220(1994) [PubMed: 7828877] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning of the pka1 gene encoding the catalytic subunit of the cAMP-dependent protein kinase in Schizosaccharomyces pombe." Maeda T., Watanabe Y., Kunitomo H., Yamamoto M. J. Biol. Chem. 269:9632-9637(1994) [PubMed: 8144551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[4]	"Phosphoproteome analysis of fission yeast." Wilson-Grady J.T., Villen J., Gygi S.P. J. Proteome Res. 7:1088-1097(2008) [PubMed: 18257517] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-356, MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U08622 mRNA. Translation: AAA70165.1. D23667 Genomic DNA. Translation: BAA04891.1. CU329671 Genomic DNA. Translation: CAB53726.1.
PIR	A54400.
RefSeq	NP_595159.1.
3D structure databases
HSSP	HSSP built from PDB template 1FOT based on UniProtKB P06244.
ModBase	Search...
Protein-protein interaction databases
STRING	P40376.
Genome annotation databases
GeneID	2539781.
GenomeReviews	Gene locus pka1 in contig CU329671_GR.
KEGG	spo:SPBC106.10.
NMPDR	fig|4896.1.peg.1025.
Organism-specific databases
GeneDB_Spombe	SPBC106.10.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-003256-MON.
BRENDA	2.7.11.11. 653.
Gene expression databases
ArrayExpress	P40376.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KAPB_SCHPO

Accession

Primary (citable) accession number: P40376

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	September 22, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)