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Protein

Enolase 1-1

Gene

eno101

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarityNote: Mg2+ is required for catalysis and for stabilizing the dimer.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 2 (gpd3), Glyceraldehyde-3-phosphate dehydrogenase 1 (tdh1)
  2. Phosphoglycerate kinase (pgk1)
  3. Phosphoglycerate mutase (gpm1)
  4. Enolase 1-2 (eno102), Enolase 1-1 (eno101)
  5. Pyruvate kinase (pyk1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei159 – 1591SubstrateBy similarity
Binding sitei168 – 1681SubstrateBy similarity
Active sitei211 – 2111Proton donorBy similarity
Metal bindingi246 – 2461MagnesiumBy similarity
Metal bindingi295 – 2951MagnesiumBy similarity
Binding sitei295 – 2951SubstrateBy similarity
Metal bindingi320 – 3201MagnesiumBy similarity
Binding sitei320 – 3201SubstrateBy similarity
Active sitei345 – 3451Proton acceptorBy similarity
Binding sitei396 – 3961SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-SPO-70171. Glycolysis.
R-SPO-70263. Gluconeogenesis.
UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase 1-1 (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase 1-1
2-phosphoglycerate dehydratase 1-1
Gene namesi
Name:eno101
Synonyms:eno1
ORF Names:SPBC1815.01
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC1815.01.
PomBaseiSPBC1815.01. eno101.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • nucleus Source: PomBase
  • phosphopyruvate hydratase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 439439Enolase 1-1PRO_0000134058Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei85 – 851Phosphothreonine1 Publication
Modified residuei249 – 2491Phosphoserine1 Publication
Modified residuei250 – 2501Phosphoserine1 Publication
Modified residuei253 – 2531Phosphotyrosine1 Publication
Modified residuei351 – 3511Phosphoserine1 Publication
Modified residuei353 – 3531Phosphothreonine1 Publication
Modified residuei355 – 3551Phosphoserine1 Publication
Modified residuei421 – 4211Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40370.

PTM databases

iPTMnetiP40370.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi276332. 8 interactions.
IntActiP40370. 5 interactions.
MINTiMINT-4689541.

Structurei

3D structure databases

ProteinModelPortaliP40370.
SMRiP40370. Positions 2-427.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni372 – 3754Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

HOGENOMiHOG000072174.
InParanoidiP40370.
KOiK01689.
OMAiDSFDATW.
OrthoDBiEOG092C2W5X.
PhylomeDBiP40370.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiIPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
[Graphical view]
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiPF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SMARTiSM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiPS00164. ENOLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40370-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIQKVFARQ IYDSRGNPTV EVDLTTETGI HRAIVPSGAS TGIWEALEMR
60 70 80 90 100
DGDKTKWGGK GVLKAVGNVN NIIAPAVVKA NLDVTDQKAA DEFLLKLDGT
110 120 130 140 150
ENKSKLGANA ILGVSMAICR AGAAQKKLPL WKYIAENFGT KGPYVLPVPS
160 170 180 190 200
FNVLNGGSHA GGDLAFQEFM ILPTGAPSFS EAMRWGAETY HTLKSIAKKR
210 220 230 240 250
YGSSAGNVGD EGGIAPDLQT PQEALDLIVE AINKAGYEGK IKIGLDVASS
260 270 280 290 300
EFYVDGKYDL DIKAAKPKPE NKLTYQQLTD LYVELSKKYP IVSIEDPFDQ
310 320 330 340 350
DDWSAWTHMK AETDFQIVGD DLTVTNVKRL RTAIDKKCAN ALLLKVNQIG
360 370 380 390 400
SVTESLNAVR MSYEAGWGVM VSHRSGETAD TFISHLTVGI GAGQLKSGAP
410 420 430
CRSERLAKYN ELLRIEEELG SEGVYAGAHA GKYIKAAKF
Length:439
Mass (Da):47,436
Last modified:January 11, 2001 - v2
Checksum:i4CA257CFF4B456E3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti13 – 153DSR → ALG in AAA70080 (PubMed:7867936).Curated
Sequence conflicti191 – 1911H → Q in AAA70080 (PubMed:7867936).Curated
Sequence conflicti248 – 2481A → T in AAA70080 (PubMed:7867936).Curated
Sequence conflicti358 – 3581A → V in AAA70080 (PubMed:7867936).Curated
Sequence conflicti377 – 3771E → G in AAA70080 (PubMed:7867936).Curated
Sequence conflicti422 – 4221E → R in AAA70080 (PubMed:7867936).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13799 mRNA. Translation: AAA70080.1.
L37084 mRNA. Translation: AAA51399.2.
CU329671 Genomic DNA. Translation: CAB43486.1.
PIRiJC4036.
T39737.
T45116.
RefSeqiNP_595903.1. NM_001021810.2.

Genome annotation databases

EnsemblFungiiSPBC1815.01.1; SPBC1815.01.1:pep; SPBC1815.01.
GeneIDi2539782.
KEGGispo:SPBC1815.01.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U13799 mRNA. Translation: AAA70080.1.
L37084 mRNA. Translation: AAA51399.2.
CU329671 Genomic DNA. Translation: CAB43486.1.
PIRiJC4036.
T39737.
T45116.
RefSeqiNP_595903.1. NM_001021810.2.

3D structure databases

ProteinModelPortaliP40370.
SMRiP40370. Positions 2-427.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi276332. 8 interactions.
IntActiP40370. 5 interactions.
MINTiMINT-4689541.

PTM databases

iPTMnetiP40370.

Proteomic databases

MaxQBiP40370.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC1815.01.1; SPBC1815.01.1:pep; SPBC1815.01.
GeneIDi2539782.
KEGGispo:SPBC1815.01.

Organism-specific databases

EuPathDBiFungiDB:SPBC1815.01.
PomBaseiSPBC1815.01. eno101.

Phylogenomic databases

HOGENOMiHOG000072174.
InParanoidiP40370.
KOiK01689.
OMAiDSFDATW.
OrthoDBiEOG092C2W5X.
PhylomeDBiP40370.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.
ReactomeiR-SPO-70171. Glycolysis.
R-SPO-70263. Gluconeogenesis.

Miscellaneous databases

PROiP40370.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiIPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
[Graphical view]
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiPF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SMARTiSM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiPS00164. ENOLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiENO11_SCHPO
AccessioniPrimary (citable) accession number: P40370
Secondary accession number(s): Q12703, Q9Y7J7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 11, 2001
Last modified: September 7, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.