P40369 (ARLY_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Argininosuccinate lyase
Short name=ASAL
EC=4.3.2.1

Alternative name(s):
Arginosuccinase

Gene names

Name:	arg7
ORF Names:	SPBC1773.14

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › Schizosaccharomyces pombe

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2-(N(omega)-L-arginino)succinate = fumarate + L-arginine.
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	arginine biosynthetic process Inferred from mutant phenotype Ref.1. Source: PomBase arginine biosynthetic process via ornithine Inferred from electronic annotation. Source: InterPro urea cycle Inferred by curator. Source: PomBase
Cellular_component	cytosol Inferred from direct assay PubMed 16823372. Source: PomBase
Molecular_function	argininosuccinate lyase activity Inferred from mutant phenotype Ref.1. Source: PomBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	Argininosuccinate lyase		PRO_0000137725
Experimental info
	Sequence conflict	199 – 200	2	PL → TA in CAA44915. Ref.1
	Sequence conflict	218	1	R → P in CAA44915. Ref.1
	Sequence conflict	223	1	F → S in CAA44915. Ref.1
	Sequence conflict	371	1	A → AS in CAA44915. Ref.1
	Sequence conflict	451 – 461	11	EQIEHIRSAIL → DNRAYTISNS in CAA44915. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P40369 [UniParc]. Last modified August 14, 2001. Version 2. Checksum: 60AA6D072C58FB86	FASTA	461	51,738
	10 20 30 40 50 60 MAEKSSKKLW GGRFSGATDP LMAEFNKSIY SGKEMCEEDV IGSMAYAKAL CQKNVISEEE 70 80 90 100 110 120 LNSILKGLEQ IQREWNSGQF VLEPSDEDVH TANERRLTEI IGDVAGKLHT GRSRNDQVTT 130 140 150 160 170 180 DLRLWLCRKI KEVEVYVINL LKVFTNRAEM EIDVIMSGYT HLQRAQPVRW SHFLMSHALP 190 200 210 220 230 240 LLGDLGRLRQ LYTRVSQLPL GAGALAGNPF NVDREFLRKE LGFEGIIMNS MNAVGDRDFV 250 260 270 280 290 300 IEFMFWAGMV MLHISRFAED LIIYSSSEFG FVTLSDAYST GSSIMPQKKN PDSLELLRGK 310 320 330 340 350 360 SGRVLGDMIG LMITVKGTPT TYNKDLQEDK EPLFDAFKTV SDSLQILTGV VSTLTINPTK 370 380 390 400 410 420 IAESLTPDLL ATDLAEYLVR KGLPFRQTHH ISGSAVRMAE ERNTTLDKLS VSDLQSLHPL 430 440 450 460 FDEDVSKVFN YEESVEKRCS IGGTAKHCVQ EQIEHIRSAI L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of the ARG7 gene of Schizosaccharomyces pombe coding for argininosuccinate lyase. Expression of the gene in Saccharomyces cerevisiae." Loppes R., Michels R., Decroupette I., Joris B. Curr. Genet. 19:255-260(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X63262 Genomic DNA. Translation: CAA44915.1. CU329671 Genomic DNA. Translation: CAA21919.1.
PIR	S32580. T39679.
RefSeq	NP_595129.1. NM_001021036.2.
3D structure databases
ProteinModelPortal	P40369.
SMR	P40369. Positions 21-460.
ModBase	Search...
Protein-protein interaction databases
STRING	4896.SPBC1773.14-1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC1773.14.1; SPBC1773.14.1:pep; SPBC1773.14.
GeneID	2539980.
KEGG	spo:SPBC1773.14.
Organism-specific databases
PomBase	SPBC1773.14.
Phylogenomic databases
eggNOG	COG0165.
HOGENOM	HOG000242744.
KO	K01755.
OMA	EDFANTH.
OrthoDB	EOG4VMJQ2.
Enzyme and pathway databases
UniPathway	UPA00068; UER00114.
Family and domain databases
Gene3D	1.10.275.10. 1 hit.
InterPro	IPR009049. Argininosuccinate_lyase. IPR003031. D_crystallin. IPR024083. Fumarase/histidase_N. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR022761. Fumarate_lyase_N. IPR008948. L-Aspartase-like. [Graphical view]
PANTHER	PTHR11444:SF3. PTHR11444:SF3. 1 hit.
Pfam	PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
SUPFAM	SSF48557. L-Aspartase-like. 1 hit.
TIGRFAMs	TIGR00838. argH. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20801123.

Entry information

Entry name

ARLY_SCHPO

Accession

Primary (citable) accession number: P40369
Secondary accession number(s): O94571

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	August 14, 2001
Last modified:	May 1, 2013
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program