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Protein

Nucleoporin NUP82

Gene

NUP82

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. It is specifically involved as part of the NUP82-NUP159-NSP1 subcomplex in nuclear mRNA and pre-ribosome export by acting as a linker tethering nucleoporins that are directly involved in nuclear transport to the NPC via its coiled-coil domain.6 Publications

GO - Molecular functioni

  • structural constituent of nuclear pore Source: SGD
  • transporter activity Source: GO_Central

GO - Biological processi

  • mRNA export from nucleus Source: SGD
  • protein export from nucleus Source: SGD
  • protein import into nucleus Source: SGD
  • ribosomal large subunit export from nucleus Source: SGD
  • ribosomal small subunit export from nucleus Source: SGD
Complete GO annotation...

Keywords - Biological processi

mRNA transport, Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31523-MONOMER.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nucleoporin NUP82
Alternative name(s):
Nuclear pore protein NUP82
Gene namesi
Name:NUP82
Ordered Locus Names:YJL061W
ORF Names:HRB187, J1135
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL061W.
SGDiS000003597. NUP82.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: SGD
  • nuclear membrane Source: UniProtKB-SubCell
  • nuclear pore Source: SGD
  • nuclear pore cytoplasmic filaments Source: SGD
  • nuclear pore linkers Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Nuclear pore complex, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002048831 – 713Nucleoporin NUP82Add BLAST713

Proteomic databases

MaxQBiP40368.
PRIDEiP40368.

Interactioni

Subunit structurei

The nuclear pore complex (NPC) constitutes the exclusive means of nucleocytoplasmic transport. NPCs allow the passive diffusion of ions and small molecules and the active, nuclear transport receptor-mediated bidirectional transport of macromolecules such as proteins, RNAs, ribonucleoparticles (RNPs), and ribosomal subunits across the nuclear envelope. The 55-60 MDa NPC is composed of at least 31 different subunits: ASM4, CDC31, GLE1, GLE2, NDC1, NIC96, NSP1, NUP1, NUP2, NUP100, NUP116, NUP120, NUP133, NUP145, NUP157, NUP159, NUP170, NUP188, NUP192, NUP42, NUP49, NUP53, NUP57, NUP60, NUP82, NUP84, NUP85, POM152, POM34, SEH1 and SEC1. Due to its 8-fold rotational symmetry, all subunits are present with 8 copies or multiples thereof. NUP82 is part of the NUP82 subcomplex (NUP82, NSP1, NUP159). This subcomplex is the base for interactions with NUP116 and GLE2 and with NUP42 and GLE1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NSP1P1490710EBI-12331,EBI-12265
NUP116Q026305EBI-12331,EBI-11703
NUP159P404776EBI-12331,EBI-11747

Protein-protein interaction databases

BioGridi33693. 60 interactors.
DIPiDIP-913N.
IntActiP40368. 22 interactors.
MINTiMINT-498618.

Structurei

Secondary structure

1713
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 8Combined sources4
Turni11 – 13Combined sources3
Beta strandi20 – 22Combined sources3
Beta strandi24 – 29Combined sources6
Turni30 – 33Combined sources4
Beta strandi34 – 39Combined sources6
Beta strandi42 – 47Combined sources6
Turni48 – 50Combined sources3
Beta strandi55 – 58Combined sources4
Turni60 – 62Combined sources3
Beta strandi70 – 73Combined sources4
Beta strandi77 – 83Combined sources7
Beta strandi85 – 92Combined sources8
Helixi103 – 107Combined sources5
Beta strandi110 – 115Combined sources6
Helixi116 – 118Combined sources3
Beta strandi119 – 122Combined sources4
Beta strandi127 – 132Combined sources6
Helixi137 – 139Combined sources3
Beta strandi141 – 146Combined sources6
Beta strandi151 – 155Combined sources5
Beta strandi164 – 167Combined sources4
Beta strandi171 – 175Combined sources5
Beta strandi182 – 187Combined sources6
Beta strandi194 – 198Combined sources5
Beta strandi204 – 210Combined sources7
Beta strandi214 – 217Combined sources4
Helixi220 – 235Combined sources16
Beta strandi239 – 241Combined sources3
Helixi243 – 262Combined sources20
Turni265 – 268Combined sources4
Beta strandi269 – 271Combined sources3
Helixi274 – 277Combined sources4
Beta strandi286 – 288Combined sources3
Helixi293 – 296Combined sources4
Beta strandi299 – 306Combined sources8
Beta strandi308 – 311Combined sources4
Beta strandi313 – 319Combined sources7
Turni320 – 322Combined sources3
Beta strandi323 – 329Combined sources7
Beta strandi347 – 355Combined sources9
Beta strandi362 – 364Combined sources3
Beta strandi372 – 376Combined sources5
Beta strandi378 – 385Combined sources8
Helixi387 – 399Combined sources13
Helixi403 – 405Combined sources3
Beta strandi413 – 419Combined sources7
Beta strandi425 – 431Combined sources7
Beta strandi434 – 440Combined sources7
Beta strandi445 – 449Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PBPX-ray2.60A/D/G/J1-452[»]
3TKNX-ray3.40A/D/G1-452[»]
ProteinModelPortaliP40368.
SMRiP40368.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40368.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 409Interaction with NUP1161 PublicationAdd BLAST409
Regioni463 – 713Interaction with NSP1 and NUP1591 PublicationAdd BLAST251

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili582 – 713Sequence analysisAdd BLAST132

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi607 – 623Bipartite nuclear localization signalAdd BLAST17

Keywords - Domaini

Coiled coil

Phylogenomic databases

HOGENOMiHOG000113891.
InParanoidiP40368.
KOiK18719.
OMAiWFKEIRN.
OrthoDBiEOG092C0QDV.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40368-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQSSRLSAL PIFQASLSAS QSPRYIFSSQ NGTRIVFIQD NIIRWYNVLT
60 70 80 90 100
DSLYHSLNFS RHLVLDDTFH VISSTSGDLL CLFNDNEIFV MEVPWGYSNV
110 120 130 140 150
EDVSIQDAFQ IFHYSIDEEE VGPKSSIKKV LFHPKSYRDS CIVVLKEDDT
160 170 180 190 200
ITMFDILNSQ EKPIVLNKPN NSFGLDARVN DITDLEFSKD GLTLYCLNTT
210 220 230 240 250
EGGDIFAFYP FLPSVLLLNE KDLNLILNKS LVMYESLDST TDVIVKRNVI
260 270 280 290 300
KQLQFVSKLH ENWNSRFGKV DIQKEYRLAK VQGPFTINPF PGELYDYTAT
310 320 330 340 350
NIATILIDNG QNEIVCVSFD DGSLILLFKD LEMSMSWDVD NYVYNNSLVL
360 370 380 390 400
IERVKLQREI KSLITLPEQL GKLYVISDNI IQQVNFMSWA STLSKCINES
410 420 430 440 450
DLNPLAGLKF ESKLEDIATI ERIPNLAYIN WNDQSNLALM SNKTLTFQNI
460 470 480 490 500
SSDMKPQSTA AETSISTEKS DTVGDGFKMS FTQPINEILI LNDNFQKACI
510 520 530 540 550
SPCERIIPSA DRQIPLKNEA SENQLEIFTD ISKEFLQRIV KAQTLGVSIH
560 570 580 590 600
NRIHEQQFEL TRQLQSTCKI ISKDDDLRRK FEAQNKKWDA QLSRQSELME
610 620 630 640 650
RFSKLSKKLS QIAESNKFKE KKISHGEMKW FKEIRNQILQ FNSFVHSQKS
660 670 680 690 700
LQQDLSYLKS ELTRIEAETI KVDKKSQNEW DELRKMLEID SKIIKECNEE
710
LLQVSQEFTT KTQ
Length:713
Mass (Da):82,085
Last modified:November 1, 1995 - v2
Checksum:iC379B5E111E26B50
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31543 Genomic DNA. Translation: AAA85504.1.
X85970 Genomic DNA. Translation: CAA59957.1.
Z49336 Genomic DNA. Translation: CAA89352.1.
Z34288 Genomic DNA. Translation: CAA84062.1.
BK006943 Genomic DNA. Translation: DAA08737.1.
PIRiS56833.
RefSeqiNP_012474.1. NM_001181494.1.

Genome annotation databases

EnsemblFungiiYJL061W; YJL061W; YJL061W.
GeneIDi853385.
KEGGisce:YJL061W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U31543 Genomic DNA. Translation: AAA85504.1.
X85970 Genomic DNA. Translation: CAA59957.1.
Z49336 Genomic DNA. Translation: CAA89352.1.
Z34288 Genomic DNA. Translation: CAA84062.1.
BK006943 Genomic DNA. Translation: DAA08737.1.
PIRiS56833.
RefSeqiNP_012474.1. NM_001181494.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PBPX-ray2.60A/D/G/J1-452[»]
3TKNX-ray3.40A/D/G1-452[»]
ProteinModelPortaliP40368.
SMRiP40368.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33693. 60 interactors.
DIPiDIP-913N.
IntActiP40368. 22 interactors.
MINTiMINT-498618.

Protein family/group databases

TCDBi1.I.1.1.1. the nuclear pore complex (npc) family.

Proteomic databases

MaxQBiP40368.
PRIDEiP40368.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL061W; YJL061W; YJL061W.
GeneIDi853385.
KEGGisce:YJL061W.

Organism-specific databases

EuPathDBiFungiDB:YJL061W.
SGDiS000003597. NUP82.

Phylogenomic databases

HOGENOMiHOG000113891.
InParanoidiP40368.
KOiK18719.
OMAiWFKEIRN.
OrthoDBiEOG092C0QDV.

Enzyme and pathway databases

BioCyciYEAST:G3O-31523-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP40368.
PROiP40368.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNUP82_YEAST
AccessioniPrimary (citable) accession number: P40368
Secondary accession number(s): D6VWC1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 9470 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.