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P40363

- SFGH_YEAST

UniProt

P40363 - SFGH_YEAST

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Protein

S-formylglutathione hydrolase

Gene

YJL068C

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Serine hydrolase involved in the detoxification of formaldehyde.1 Publication

Catalytic activityi

S-formylglutathione + H2O = glutathione + formate.

pH dependencei

Optimum pH is 7.1 Publication

Temperature dependencei

Optimum temperature is 50 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei161 – 1611Charge relay systemBy similarity
Active sitei241 – 2411Charge relay systemBy similarity
Active sitei276 – 2761Charge relay systemBy similarity

GO - Molecular functioni

  1. carboxylic ester hydrolase activity Source: UniProtKB-KW
  2. S-formylglutathione hydrolase activity Source: SGD

GO - Biological processi

  1. formaldehyde catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Names & Taxonomyi

Protein namesi
Recommended name:
S-formylglutathione hydrolase (EC:3.1.2.12)
Short name:
FGH
Gene namesi
Ordered Locus Names:YJL068C
ORF Names:HRE299, J1102
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL068c.
SGDiS000003604. YJL068C.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytosol Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 299299S-formylglutathione hydrolasePRO_0000210342Add
BLAST

Proteomic databases

MaxQBiP40363.
PaxDbiP40363.
PeptideAtlasiP40363.

Expressioni

Gene expression databases

GenevestigatoriP40363.

Interactioni

Subunit structurei

Monomer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself1EBI-25922,EBI-25922
CYS4P325821EBI-25922,EBI-4167
TAL1P150191EBI-25922,EBI-18952
YHR080CP388001EBI-25922,EBI-24597

Protein-protein interaction databases

BioGridi33687. 63 interactions.
DIPiDIP-4988N.
MINTiMINT-540859.
STRINGi4932.YJL068C.

Structurei

Secondary structure

1
299
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 98Combined sources
Beta strandi12 – 209Combined sources
Turni22 – 243Combined sources
Beta strandi25 – 3410Combined sources
Helixi36 – 394Combined sources
Beta strandi51 – 555Combined sources
Helixi62 – 687Combined sources
Helixi71 – 788Combined sources
Beta strandi81 – 855Combined sources
Beta strandi102 – 1054Combined sources
Helixi117 – 1204Combined sources
Helixi125 – 1306Combined sources
Helixi132 – 14110Combined sources
Beta strandi151 – 16010Combined sources
Helixi162 – 17312Combined sources
Helixi175 – 1773Combined sources
Beta strandi181 – 1877Combined sources
Helixi192 – 1943Combined sources
Helixi196 – 20510Combined sources
Helixi214 – 2163Combined sources
Helixi218 – 2214Combined sources
Helixi222 – 2243Combined sources
Beta strandi233 – 2386Combined sources
Helixi244 – 2474Combined sources
Helixi251 – 2577Combined sources
Turni261 – 2644Combined sources
Beta strandi266 – 2716Combined sources
Helixi278 – 29518Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PV1X-ray2.30A/B/C/D1-299[»]
3C6BX-ray2.17A1-299[»]
4FLMX-ray2.41A/B1-299[»]
4FOLX-ray2.07A/B/C/D1-299[»]
ProteinModelPortaliP40363.
SMRiP40363. Positions 1-299.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40363.

Family & Domainsi

Sequence similaritiesi

Belongs to the esterase D family.Curated

Phylogenomic databases

eggNOGiCOG0627.
GeneTreeiENSGT00390000011864.
HOGENOMiHOG000263929.
InParanoidiP40363.
KOiK01070.
OMAiRNREHYQ.
OrthoDBiEOG73Z34V.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000801. Esterase_put.
IPR014186. S-formylglutathione_hydrol.
[Graphical view]
PANTHERiPTHR10061. PTHR10061. 1 hit.
PfamiPF00756. Esterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR02821. fghA_ester_D. 1 hit.

Sequencei

Sequence statusi: Complete.

P40363-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVVKEFSVC GGRLIKLSHN SNSTKTSMNV NIYLPKHYYA QDFPRNKRIP
60 70 80 90 100
TVFYLSGLTC TPDNASEKAF WQFQADKYGF AIVFPDTSPR GDEVANDPEG
110 120 130 140 150
SWDFGQGAGF YLNATQEPYA QHYQMYDYIH KELPQTLDSH FNKNGDVKLD
160 170 180 190 200
FLDNVAITGH SMGGYGAICG YLKGYSGKRY KSCSAFAPIV NPSNVPWGQK
210 220 230 240 250
AFKGYLGEEK AQWEAYDPCL LIKNIRHVGD DRILIHVGDS DPFLEEHLKP
260 270 280 290
ELLLEAVKAT SWQDYVEIKK VHGFDHSYYF VSTFVPEHAE FHARNLGLI
Length:299
Mass (Da):33,934
Last modified:February 1, 1995 - v1
Checksum:iBFA5DC02729E9AEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34288 Genomic DNA. Translation: CAA84054.1.
Z49343 Genomic DNA. Translation: CAA89359.1.
X88851 Genomic DNA. Translation: CAA61307.1.
BK006943 Genomic DNA. Translation: DAA08731.1.
PIRiS50803.
RefSeqiNP_012467.1. NM_001181501.1.

Genome annotation databases

EnsemblFungiiYJL068C; YJL068C; YJL068C.
GeneIDi853377.
KEGGisce:YJL068C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34288 Genomic DNA. Translation: CAA84054.1 .
Z49343 Genomic DNA. Translation: CAA89359.1 .
X88851 Genomic DNA. Translation: CAA61307.1 .
BK006943 Genomic DNA. Translation: DAA08731.1 .
PIRi S50803.
RefSeqi NP_012467.1. NM_001181501.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1PV1 X-ray 2.30 A/B/C/D 1-299 [» ]
3C6B X-ray 2.17 A 1-299 [» ]
4FLM X-ray 2.41 A/B 1-299 [» ]
4FOL X-ray 2.07 A/B/C/D 1-299 [» ]
ProteinModelPortali P40363.
SMRi P40363. Positions 1-299.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33687. 63 interactions.
DIPi DIP-4988N.
MINTi MINT-540859.
STRINGi 4932.YJL068C.

Proteomic databases

MaxQBi P40363.
PaxDbi P40363.
PeptideAtlasi P40363.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJL068C ; YJL068C ; YJL068C .
GeneIDi 853377.
KEGGi sce:YJL068C.

Organism-specific databases

CYGDi YJL068c.
SGDi S000003604. YJL068C.

Phylogenomic databases

eggNOGi COG0627.
GeneTreei ENSGT00390000011864.
HOGENOMi HOG000263929.
InParanoidi P40363.
KOi K01070.
OMAi RNREHYQ.
OrthoDBi EOG73Z34V.

Miscellaneous databases

EvolutionaryTracei P40363.
NextBioi 973828.
PROi P40363.

Gene expression databases

Genevestigatori P40363.

Family and domain databases

Gene3Di 3.40.50.1820. 1 hit.
InterProi IPR029058. AB_hydrolase.
IPR000801. Esterase_put.
IPR014186. S-formylglutathione_hydrol.
[Graphical view ]
PANTHERi PTHR10061. PTHR10061. 1 hit.
Pfami PF00756. Esterase. 1 hit.
[Graphical view ]
SUPFAMi SSF53474. SSF53474. 1 hit.
TIGRFAMsi TIGR02821. fghA_ester_D. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of a 17.1 kb DNA fragment from chromosome X of Saccharomyces cerevisiae includes the mitochondrial ribosomal protein L8."
    Vandenbol M., Durand P., Dion C., Portetelle D., Hilger F.
    Yeast 11:57-60(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Purification and properties of an esterase from the yeast Saccharomyces cerevisiae and identification of the encoding gene."
    Degrassi G., Uotila L., Klima R., Venturi V.
    Appl. Environ. Microbiol. 65:3470-3472(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-7, FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSFGH_YEAST
AccessioniPrimary (citable) accession number: P40363
Secondary accession number(s): D6VWB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 26, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1750 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3