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P40363

- SFGH_YEAST

UniProt

P40363 - SFGH_YEAST

Protein

S-formylglutathione hydrolase

Gene

YJL068C

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Serine hydrolase involved in the detoxification of formaldehyde.1 Publication

    Catalytic activityi

    S-formylglutathione + H2O = glutathione + formate.

    pH dependencei

    Optimum pH is 7.1 Publication

    Temperature dependencei

    Optimum temperature is 50 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei161 – 1611Charge relay systemBy similarity
    Active sitei241 – 2411Charge relay systemBy similarity
    Active sitei276 – 2761Charge relay systemBy similarity

    GO - Molecular functioni

    1. S-formylglutathione hydrolase activity Source: SGD

    GO - Biological processi

    1. formaldehyde catabolic process Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Serine esterase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    S-formylglutathione hydrolase (EC:3.1.2.12)
    Short name:
    FGH
    Gene namesi
    Ordered Locus Names:YJL068C
    ORF Names:HRE299, J1102
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL068c.
    SGDiS000003604. YJL068C.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytosol Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 299299S-formylglutathione hydrolasePRO_0000210342Add
    BLAST

    Proteomic databases

    MaxQBiP40363.
    PaxDbiP40363.
    PeptideAtlasiP40363.

    Expressioni

    Gene expression databases

    GenevestigatoriP40363.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself1EBI-25922,EBI-25922
    CYS4P325821EBI-25922,EBI-4167
    TAL1P150191EBI-25922,EBI-18952
    YHR080CP388001EBI-25922,EBI-24597

    Protein-protein interaction databases

    BioGridi33687. 62 interactions.
    DIPiDIP-4988N.
    MINTiMINT-540859.
    STRINGi4932.YJL068C.

    Structurei

    Secondary structure

    1
    299
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 98
    Beta strandi12 – 209
    Turni22 – 243
    Beta strandi25 – 3410
    Helixi36 – 394
    Beta strandi51 – 555
    Helixi62 – 687
    Helixi71 – 788
    Beta strandi81 – 855
    Beta strandi102 – 1054
    Helixi117 – 1204
    Helixi125 – 1306
    Helixi132 – 14110
    Beta strandi151 – 16010
    Helixi162 – 17312
    Helixi175 – 1773
    Beta strandi181 – 1877
    Helixi192 – 1943
    Helixi196 – 20510
    Helixi214 – 2163
    Helixi218 – 2214
    Helixi222 – 2243
    Beta strandi233 – 2386
    Helixi244 – 2474
    Helixi251 – 2577
    Turni261 – 2644
    Beta strandi266 – 2716
    Helixi278 – 29518

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1PV1X-ray2.30A/B/C/D1-299[»]
    3C6BX-ray2.17A1-299[»]
    4FLMX-ray2.41A/B1-299[»]
    4FOLX-ray2.07A/B/C/D1-299[»]
    ProteinModelPortaliP40363.
    SMRiP40363. Positions 1-299.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP40363.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the esterase D family.Curated

    Phylogenomic databases

    eggNOGiCOG0627.
    GeneTreeiENSGT00390000011864.
    HOGENOMiHOG000263929.
    KOiK01070.
    OMAiRNREHYQ.
    OrthoDBiEOG73Z34V.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR000801. Esterase_put.
    IPR014186. S-formylglutathione_hydrol.
    [Graphical view]
    PANTHERiPTHR10061. PTHR10061. 1 hit.
    PfamiPF00756. Esterase. 1 hit.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.
    TIGRFAMsiTIGR02821. fghA_ester_D. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P40363-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVVKEFSVC GGRLIKLSHN SNSTKTSMNV NIYLPKHYYA QDFPRNKRIP    50
    TVFYLSGLTC TPDNASEKAF WQFQADKYGF AIVFPDTSPR GDEVANDPEG 100
    SWDFGQGAGF YLNATQEPYA QHYQMYDYIH KELPQTLDSH FNKNGDVKLD 150
    FLDNVAITGH SMGGYGAICG YLKGYSGKRY KSCSAFAPIV NPSNVPWGQK 200
    AFKGYLGEEK AQWEAYDPCL LIKNIRHVGD DRILIHVGDS DPFLEEHLKP 250
    ELLLEAVKAT SWQDYVEIKK VHGFDHSYYF VSTFVPEHAE FHARNLGLI 299
    Length:299
    Mass (Da):33,934
    Last modified:February 1, 1995 - v1
    Checksum:iBFA5DC02729E9AEF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z34288 Genomic DNA. Translation: CAA84054.1.
    Z49343 Genomic DNA. Translation: CAA89359.1.
    X88851 Genomic DNA. Translation: CAA61307.1.
    BK006943 Genomic DNA. Translation: DAA08731.1.
    PIRiS50803.
    RefSeqiNP_012467.1. NM_001181501.1.

    Genome annotation databases

    EnsemblFungiiYJL068C; YJL068C; YJL068C.
    GeneIDi853377.
    KEGGisce:YJL068C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z34288 Genomic DNA. Translation: CAA84054.1 .
    Z49343 Genomic DNA. Translation: CAA89359.1 .
    X88851 Genomic DNA. Translation: CAA61307.1 .
    BK006943 Genomic DNA. Translation: DAA08731.1 .
    PIRi S50803.
    RefSeqi NP_012467.1. NM_001181501.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1PV1 X-ray 2.30 A/B/C/D 1-299 [» ]
    3C6B X-ray 2.17 A 1-299 [» ]
    4FLM X-ray 2.41 A/B 1-299 [» ]
    4FOL X-ray 2.07 A/B/C/D 1-299 [» ]
    ProteinModelPortali P40363.
    SMRi P40363. Positions 1-299.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33687. 62 interactions.
    DIPi DIP-4988N.
    MINTi MINT-540859.
    STRINGi 4932.YJL068C.

    Proteomic databases

    MaxQBi P40363.
    PaxDbi P40363.
    PeptideAtlasi P40363.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL068C ; YJL068C ; YJL068C .
    GeneIDi 853377.
    KEGGi sce:YJL068C.

    Organism-specific databases

    CYGDi YJL068c.
    SGDi S000003604. YJL068C.

    Phylogenomic databases

    eggNOGi COG0627.
    GeneTreei ENSGT00390000011864.
    HOGENOMi HOG000263929.
    KOi K01070.
    OMAi RNREHYQ.
    OrthoDBi EOG73Z34V.

    Miscellaneous databases

    EvolutionaryTracei P40363.
    NextBioi 973828.
    PROi P40363.

    Gene expression databases

    Genevestigatori P40363.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR000801. Esterase_put.
    IPR014186. S-formylglutathione_hydrol.
    [Graphical view ]
    PANTHERi PTHR10061. PTHR10061. 1 hit.
    Pfami PF00756. Esterase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53474. SSF53474. 1 hit.
    TIGRFAMsi TIGR02821. fghA_ester_D. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of a 17.1 kb DNA fragment from chromosome X of Saccharomyces cerevisiae includes the mitochondrial ribosomal protein L8."
      Vandenbol M., Durand P., Dion C., Portetelle D., Hilger F.
      Yeast 11:57-60(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Purification and properties of an esterase from the yeast Saccharomyces cerevisiae and identification of the encoding gene."
      Degrassi G., Uotila L., Klima R., Venturi V.
      Appl. Environ. Microbiol. 65:3470-3472(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-7, FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSFGH_YEAST
    AccessioniPrimary (citable) accession number: P40363
    Secondary accession number(s): D6VWB5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1750 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3