Reviewed, UniProtKB/Swiss-Prot P40363 (SFGH_YEAST)

Names and origin

Protein names

Recommended name:
    S-formylglutathione hydrolase
      Short name=FGH
    EC=3.1.2.12

Gene names

Ordered Locus Names:	YJL068C
ORF Names:	J1102, HRE299

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Serine hydrolase involved in the detoxification of formaldehyde. Ref.3
Catalytic activity	S-formylglutathione + H2O = glutathione + formate.
Subunit structure	Monomer. Ref.3
Subcellular location	Cytoplasm. Ref.3
Miscellaneous	Present with 1750 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the esterase D family.
biophysicochemical properties	pH dependence: Optimum pH is 7. Temperature dependence: Optimum temperature is 50 degrees Celsius.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase Serine esterase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	formaldehyde catabolic process Ref.3 Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasmic membrane-bounded vesicle Inferred from electronic annotation. Source: InterPro cytosol Ref.3 Inferred from direct assay. Source: SGD
Molecular function	S-formylglutathione hydrolase activity Ref.3 Inferred from direct assay. Source: SGD carboxylesterase activity Inferred from electronic annotation. Source: UniProtKB-KW identical protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-25922,EBI-25922
	P38800	1	EBI-25922,EBI-24597
CYS4	P32582	1	EBI-25922,EBI-4167
TAL1	P15019	1	EBI-25922,EBI-18952

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 299

299

S-formylglutathione hydrolase

PRO_0000210342

Sites

Active site

161

1

Charge relay system By similarity

Active site

241

1

Charge relay system By similarity

Active site

276

1

Charge relay system By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

299

Helix Strand Turn

Details...

Beta strand

2 – 9

8

Beta strand

12 – 20

9

Beta strand

22 – 34

13

Turn

36 – 39

4

Beta strand

51 – 55

5

Helix

63 – 68

6

Helix

71 – 78

8

Beta strand

81 – 84

4

Beta strand

102 – 107

6

Helix

117 – 120

4

Helix

125 – 130

6

Helix

132 – 141

10

Beta strand

151 – 160

10

Helix

162 – 173

12

Helix

175 – 177

3

Beta strand

181 – 187

7

Helix

196 – 205

10

Helix

214 – 216

3

Helix

218 – 221

4

Helix

222 – 224

3

Beta strand

233 – 236

4

Turn

242 – 247

6

Helix

251 – 257

7

Turn

261 – 264

4

Beta strand

265 – 269

5

Helix

278 – 295

18

Sequences

	Sequence		Length	Mass (Da)
	P40363-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: BFA5DC02729E9AEF	FASTA	299	33,934
	10 20 30 40 50 60 MKVVKEFSVC GGRLIKLSHN SNSTKTSMNV NIYLPKHYYA QDFPRNKRIP TVFYLSGLTC 70 80 90 100 110 120 TPDNASEKAF WQFQADKYGF AIVFPDTSPR GDEVANDPEG SWDFGQGAGF YLNATQEPYA 130 140 150 160 170 180 QHYQMYDYIH KELPQTLDSH FNKNGDVKLD FLDNVAITGH SMGGYGAICG YLKGYSGKRY 190 200 210 220 230 240 KSCSAFAPIV NPSNVPWGQK AFKGYLGEEK AQWEAYDPCL LIKNIRHVGD DRILIHVGDS 250 260 270 280 290 DPFLEEHLKP ELLLEAVKAT SWQDYVEIKK VHGFDHSYYF VSTFVPEHAE FHARNLGLI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of a 17.1 kb DNA fragment from chromosome X of Saccharomyces cerevisiae includes the mitochondrial ribosomal protein L8." Vandenbol M., Durand P., Dion C., Portetelle D., Hilger F. Yeast 11:57-60(1995) [PubMed: 7762302] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Purification and properties of an esterase from the yeast Saccharomyces cerevisiae and identification of the encoding gene." Degrassi G., Uotila L., Klima R., Venturi V. Appl. Environ. Microbiol. 65:3470-3472(1999) [PubMed: 10427036] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-7, FUNCTION, SUBCELLULAR LOCATION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

Z34288 Genomic DNA. Translation: CAA84054.1.
Z49343 Genomic DNA. Translation: CAA89359.1.
X88851 Genomic DNA. Translation: CAA61307.1.

PIR

S50803.

RefSeq

NP_012467.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1PV1	X-ray	2.30	A/B/C/D	1-299	[»]
3C6B	X-ray	2.17	A	1-299	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:4988N.

IntAct

P40363. 6 interactions.

Proteomic databases

PeptideAtlas

P40363.

PRIDE

P40363.

Genome annotation databases

Ensembl

YJL068C. Saccharomyces cerevisiae. [Contig view]

GeneID

853377.

GenomeReviews

Gene locus YJL068C in contig Y13136_GR.

KEGG

sce:YJL068C.

NMPDR

fig|4932.3.peg.3438.

Organism-specific databases

CYGD

YJL068c.

SGD

S000003604. YJL068C.

Yeast-GFP

Search...

Phylogenomic databases

HOGENOM

P40363.

OMA

P40363. HLRFHAE.

Enzyme and pathway databases

BRENDA

3.1.2.12. 250.

Gene expression databases

GermOnline

YJL068C. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR000801. Esterase_put.
IPR014186. S-formylglutathione_hydrol.
[Graphical view]

PANTHER

PTHR10061. S-formylglutathione_hydrol. 1 hit.

Pfam

PF00756. Esterase. 1 hit.
[Graphical view]

TIGRFAMs

TIGR02821. fghA_ester_D. 1 hit.

ProtoNet

Search...

Other Resources

NextBio

973828.

Entry information

Entry name

SFGH_YEAST

Accession

Primary (citable) accession number: P40363

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	June 16, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)