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Protein

S-formylglutathione hydrolase

Gene

YJL068C

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Serine hydrolase involved in the detoxification of formaldehyde.1 Publication

Catalytic activityi

S-formylglutathione + H2O = glutathione + formate.

pH dependencei

Optimum pH is 7.1 Publication

Temperature dependencei

Optimum temperature is 50 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei161Charge relay systemBy similarity1
Active sitei241Charge relay systemBy similarity1
Active sitei276Charge relay systemBy similarity1

GO - Molecular functioni

  • carboxylic ester hydrolase activity Source: UniProtKB-KW
  • S-formylglutathione hydrolase activity Source: SGD

GO - Biological processi

  • formaldehyde catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Serine esterase

Enzyme and pathway databases

BRENDAi3.1.2.12. 984.
ReactomeiR-SCE-156590. Glutathione conjugation.

Protein family/group databases

ESTHERiyeast-yjg8. A85-EsteraseD-FGH.

Names & Taxonomyi

Protein namesi
Recommended name:
S-formylglutathione hydrolase (EC:3.1.2.12)
Short name:
FGH
Gene namesi
Ordered Locus Names:YJL068C
ORF Names:HRE299, J1102
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL068C.
SGDiS000003604. YJL068C.

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

  • cytosol Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002103421 – 299S-formylglutathione hydrolaseAdd BLAST299

Proteomic databases

MaxQBiP40363.
PRIDEiP40363.
TopDownProteomicsiP40363.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

BioGridi33687. 62 interactors.
DIPiDIP-4988N.
MINTiMINT-540859.

Structurei

Secondary structure

1299
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 9Combined sources8
Beta strandi12 – 20Combined sources9
Turni22 – 24Combined sources3
Beta strandi25 – 34Combined sources10
Helixi36 – 39Combined sources4
Beta strandi51 – 55Combined sources5
Helixi62 – 68Combined sources7
Helixi71 – 78Combined sources8
Beta strandi81 – 85Combined sources5
Beta strandi102 – 105Combined sources4
Helixi117 – 120Combined sources4
Helixi125 – 130Combined sources6
Helixi132 – 141Combined sources10
Beta strandi151 – 160Combined sources10
Helixi162 – 173Combined sources12
Helixi175 – 177Combined sources3
Beta strandi181 – 187Combined sources7
Helixi192 – 194Combined sources3
Helixi196 – 205Combined sources10
Helixi214 – 216Combined sources3
Helixi218 – 221Combined sources4
Helixi222 – 224Combined sources3
Beta strandi233 – 238Combined sources6
Helixi244 – 247Combined sources4
Helixi251 – 257Combined sources7
Turni261 – 264Combined sources4
Beta strandi266 – 271Combined sources6
Helixi278 – 295Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PV1X-ray2.30A/B/C/D1-299[»]
3C6BX-ray2.17A1-299[»]
4FLMX-ray2.41A/B1-299[»]
4FOLX-ray2.07A/B/C/D1-299[»]
ProteinModelPortaliP40363.
SMRiP40363.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40363.

Family & Domainsi

Sequence similaritiesi

Belongs to the esterase D family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000011864.
HOGENOMiHOG000263929.
InParanoidiP40363.
KOiK01070.
OMAiKWHAERL.
OrthoDBiEOG092C3J7W.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000801. Esterase_put.
IPR014186. S-formylglutathione_hydrol.
[Graphical view]
PANTHERiPTHR10061. PTHR10061. 1 hit.
PfamiPF00756. Esterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR02821. fghA_ester_D. 1 hit.

Sequencei

Sequence statusi: Complete.

P40363-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVVKEFSVC GGRLIKLSHN SNSTKTSMNV NIYLPKHYYA QDFPRNKRIP
60 70 80 90 100
TVFYLSGLTC TPDNASEKAF WQFQADKYGF AIVFPDTSPR GDEVANDPEG
110 120 130 140 150
SWDFGQGAGF YLNATQEPYA QHYQMYDYIH KELPQTLDSH FNKNGDVKLD
160 170 180 190 200
FLDNVAITGH SMGGYGAICG YLKGYSGKRY KSCSAFAPIV NPSNVPWGQK
210 220 230 240 250
AFKGYLGEEK AQWEAYDPCL LIKNIRHVGD DRILIHVGDS DPFLEEHLKP
260 270 280 290
ELLLEAVKAT SWQDYVEIKK VHGFDHSYYF VSTFVPEHAE FHARNLGLI
Length:299
Mass (Da):33,934
Last modified:February 1, 1995 - v1
Checksum:iBFA5DC02729E9AEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34288 Genomic DNA. Translation: CAA84054.1.
Z49343 Genomic DNA. Translation: CAA89359.1.
X88851 Genomic DNA. Translation: CAA61307.1.
BK006943 Genomic DNA. Translation: DAA08731.1.
PIRiS50803.
RefSeqiNP_012467.1. NM_001181501.1.

Genome annotation databases

EnsemblFungiiYJL068C; YJL068C; YJL068C.
GeneIDi853377.
KEGGisce:YJL068C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z34288 Genomic DNA. Translation: CAA84054.1.
Z49343 Genomic DNA. Translation: CAA89359.1.
X88851 Genomic DNA. Translation: CAA61307.1.
BK006943 Genomic DNA. Translation: DAA08731.1.
PIRiS50803.
RefSeqiNP_012467.1. NM_001181501.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PV1X-ray2.30A/B/C/D1-299[»]
3C6BX-ray2.17A1-299[»]
4FLMX-ray2.41A/B1-299[»]
4FOLX-ray2.07A/B/C/D1-299[»]
ProteinModelPortaliP40363.
SMRiP40363.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33687. 62 interactors.
DIPiDIP-4988N.
MINTiMINT-540859.

Protein family/group databases

ESTHERiyeast-yjg8. A85-EsteraseD-FGH.

Proteomic databases

MaxQBiP40363.
PRIDEiP40363.
TopDownProteomicsiP40363.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL068C; YJL068C; YJL068C.
GeneIDi853377.
KEGGisce:YJL068C.

Organism-specific databases

EuPathDBiFungiDB:YJL068C.
SGDiS000003604. YJL068C.

Phylogenomic databases

GeneTreeiENSGT00390000011864.
HOGENOMiHOG000263929.
InParanoidiP40363.
KOiK01070.
OMAiKWHAERL.
OrthoDBiEOG092C3J7W.

Enzyme and pathway databases

BRENDAi3.1.2.12. 984.
ReactomeiR-SCE-156590. Glutathione conjugation.

Miscellaneous databases

EvolutionaryTraceiP40363.
PROiP40363.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000801. Esterase_put.
IPR014186. S-formylglutathione_hydrol.
[Graphical view]
PANTHERiPTHR10061. PTHR10061. 1 hit.
PfamiPF00756. Esterase. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
TIGRFAMsiTIGR02821. fghA_ester_D. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSFGH_YEAST
AccessioniPrimary (citable) accession number: P40363
Secondary accession number(s): D6VWB5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1750 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.