ID   PSF2_YEAST              Reviewed;         213 AA.
AC   P40359; D6VWB1;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   27-MAR-2024, entry version 172.
DE   RecName: Full=DNA replication complex GINS protein PSF2;
DE   AltName: Full=Partner of Sld five 2;
GN   Name=PSF2; OrderedLocusNames=YJL072C; ORFNames=HRF213, J1086;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, IDENTIFICATION IN THE GINS
RP   COMPLEX, AND SUBCELLULAR LOCATION.
RX   PubMed=12730134; DOI=10.1101/gad.1065903;
RA   Takayama Y., Kamimura Y., Okawa M., Muramatsu S., Sugino A., Araki H.;
RT   "GINS, a novel multiprotein complex required for chromosomal DNA
RT   replication in budding yeast.";
RL   Genes Dev. 17:1153-1165(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RA   Sor F.J.;
RL   Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7762302; DOI=10.1002/yea.320110108;
RA   Vandenbol M., Durand P., Dion C., Portetelle D., Hilger F.;
RT   "Sequence of a 17.1 kb DNA fragment from chromosome X of Saccharomyces
RT   cerevisiae includes the mitochondrial ribosomal protein L8.";
RL   Yeast 11:57-60(1995).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA   Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA   Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA   Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA   Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA   Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA   Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA   Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA   Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA   Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA   To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA   von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT   "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL   EMBO J. 15:2031-2049(1996).
RN   [5]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND INTERACTION WITH PSF1 AND SLD5.
RX   PubMed=14690591; DOI=10.1016/s1097-2765(03)00476-3;
RA   Hazbun T.R., Malmstroem L., Anderson S., Graczyk B.J., Fox B., Riffle M.,
RA   Sundin B.A., Aranda J.D., McDonald W.H., Chiu C.-H., Snydsman B.E.,
RA   Bradley P., Muller E.G.D., Fields S., Baker D., Yates J.R. III, Davis T.N.;
RT   "Assigning function to yeast proteins by integration of technologies.";
RL   Mol. Cell 12:1353-1365(2003).
RN   [6]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
CC   -!- FUNCTION: Functions as part of the GINS complex which plays an
CC       essential role in the initiation of DNA replication by binding to DNA
CC       replication origins and facilitating the assembly of the DNA
CC       replication machinery. {ECO:0000269|PubMed:12730134}.
CC   -!- SUBUNIT: Component of the GINS complex which is a heterotetramer of
CC       SLD5, PSF1, PSF2 and PSF3. Interacts with PSF1 and SLD5.
CC       {ECO:0000269|PubMed:12730134, ECO:0000269|PubMed:14690591}.
CC   -!- INTERACTION:
CC       P40359; Q12488: PSF1; NbExp=5; IntAct=EBI-25936, EBI-22066;
CC       P40359; Q12146: PSF3; NbExp=5; IntAct=EBI-25936, EBI-30392;
CC       P40359; Q03406: SLD5; NbExp=7; IntAct=EBI-25936, EBI-37437;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12730134}.
CC   -!- SIMILARITY: Belongs to the GINS2/PSF2 family. {ECO:0000305}.
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DR   EMBL; X88851; CAA61311.1; -; Genomic_DNA.
DR   EMBL; Z34288; CAA84050.1; -; Genomic_DNA.
DR   EMBL; Z49347; CAA89364.1; -; Genomic_DNA.
DR   EMBL; AY558248; AAS56574.1; -; Genomic_DNA.
DR   EMBL; BK006943; DAA08727.1; -; Genomic_DNA.
DR   PIR; S50799; S50799.
DR   RefSeq; NP_012463.1; NM_001181505.1.
DR   PDB; 3JC5; EM; 4.70 A; B=1-213.
DR   PDB; 3JC6; EM; 3.70 A; B=1-213.
DR   PDB; 3JC7; EM; 4.80 A; B=1-213.
DR   PDB; 5U8S; EM; 6.10 A; B=1-213.
DR   PDB; 5U8T; EM; 4.90 A; B=1-213.
DR   PDB; 6HV9; EM; 4.98 A; D=1-213.
DR   PDB; 6PTJ; EM; 3.80 A; B=1-213.
DR   PDB; 6PTN; EM; 5.80 A; B/b=1-213.
DR   PDB; 6PTO; EM; 7.00 A; B/b/o=1-213.
DR   PDB; 6SKL; EM; 3.70 A; B=1-213.
DR   PDB; 6U0M; EM; 3.90 A; B=3-200.
DR   PDB; 7PMK; EM; 3.20 A; B=1-213.
DR   PDB; 7PMN; EM; 3.20 A; B=1-213.
DR   PDB; 8B9A; EM; 3.50 A; D=1-213.
DR   PDB; 8B9B; EM; 3.50 A; D=1-213.
DR   PDB; 8B9C; EM; 4.60 A; D=1-213.
DR   PDB; 8KG6; EM; 3.07 A; B=1-213.
DR   PDB; 8KG8; EM; 4.23 A; B=1-213.
DR   PDB; 8KG9; EM; 4.52 A; B=1-213.
DR   PDB; 8W7M; EM; 4.12 A; B=1-213.
DR   PDBsum; 3JC5; -.
DR   PDBsum; 3JC6; -.
DR   PDBsum; 3JC7; -.
DR   PDBsum; 5U8S; -.
DR   PDBsum; 5U8T; -.
DR   PDBsum; 6HV9; -.
DR   PDBsum; 6PTJ; -.
DR   PDBsum; 6PTN; -.
DR   PDBsum; 6PTO; -.
DR   PDBsum; 6SKL; -.
DR   PDBsum; 6U0M; -.
DR   PDBsum; 7PMK; -.
DR   PDBsum; 7PMN; -.
DR   PDBsum; 8B9A; -.
DR   PDBsum; 8B9B; -.
DR   PDBsum; 8B9C; -.
DR   PDBsum; 8KG6; -.
DR   PDBsum; 8KG8; -.
DR   PDBsum; 8KG9; -.
DR   PDBsum; 8W7M; -.
DR   AlphaFoldDB; P40359; -.
DR   EMDB; EMD-0288; -.
DR   EMDB; EMD-10227; -.
DR   EMDB; EMD-13537; -.
DR   EMDB; EMD-13539; -.
DR   EMDB; EMD-15924; -.
DR   EMDB; EMD-20471; -.
DR   EMDB; EMD-20472; -.
DR   EMDB; EMD-20473; -.
DR   EMDB; EMD-20607; -.
DR   EMDB; EMD-8518; -.
DR   EMDB; EMD-8519; -.
DR   SMR; P40359; -.
DR   BioGRID; 33683; 199.
DR   ComplexPortal; CPX-1641; GINS complex.
DR   DIP; DIP-3858N; -.
DR   IntAct; P40359; 7.
DR   MINT; P40359; -.
DR   STRING; 4932.YJL072C; -.
DR   MaxQB; P40359; -.
DR   PaxDb; 4932-YJL072C; -.
DR   PeptideAtlas; P40359; -.
DR   EnsemblFungi; YJL072C_mRNA; YJL072C; YJL072C.
DR   GeneID; 853373; -.
DR   KEGG; sce:YJL072C; -.
DR   AGR; SGD:S000003608; -.
DR   SGD; S000003608; PSF2.
DR   VEuPathDB; FungiDB:YJL072C; -.
DR   eggNOG; KOG4071; Eukaryota.
DR   GeneTree; ENSGT00390000007838; -.
DR   HOGENOM; CLU_078274_1_1_1; -.
DR   InParanoid; P40359; -.
DR   OMA; DSLNCMY; -.
DR   OrthoDB; 5487178at2759; -.
DR   BioCyc; YEAST:G3O-31530-MONOMER; -.
DR   Reactome; R-SCE-176974; Unwinding of DNA.
DR   BioGRID-ORCS; 853373; 3 hits in 10 CRISPR screens.
DR   PRO; PR:P40359; -.
DR   Proteomes; UP000002311; Chromosome X.
DR   RNAct; P40359; Protein.
DR   GO; GO:0071162; C:CMG complex; IDA:SGD.
DR   GO; GO:0031261; C:DNA replication preinitiation complex; IPI:SGD.
DR   GO; GO:0000811; C:GINS complex; IPI:SGD.
DR   GO; GO:0043596; C:nuclear replication fork; IDA:SGD.
DR   GO; GO:0005634; C:nucleus; IDA:SGD.
DR   GO; GO:0031298; C:replication fork protection complex; IDA:SGD.
DR   GO; GO:0006268; P:DNA unwinding involved in DNA replication; IDA:ComplexPortal.
DR   GO; GO:0006261; P:DNA-templated DNA replication; IMP:SGD.
DR   GO; GO:0000727; P:double-strand break repair via break-induced replication; IMP:SGD.
DR   CDD; cd11712; GINS_A_psf2; 1.
DR   CDD; cd21694; GINS_B_Psf2; 1.
DR   Gene3D; 1.20.58.1020; -; 1.
DR   Gene3D; 3.40.5.50; -; 1.
DR   InterPro; IPR021151; GINS_A.
DR   InterPro; IPR036224; GINS_bundle-like_dom_sf.
DR   InterPro; IPR007257; GINS_Psf2.
DR   PANTHER; PTHR12772; DNA REPLICATION COMPLEX GINS PROTEIN PSF2; 1.
DR   PANTHER; PTHR12772:SF0; DNA REPLICATION COMPLEX GINS PROTEIN PSF2; 1.
DR   Pfam; PF05916; Sld5; 1.
DR   PIRSF; PIRSF028998; GINS_Psf2_subgr; 1.
DR   SUPFAM; SSF158573; GINS helical bundle-like; 1.
DR   SUPFAM; SSF160059; PriA/YqbF domain; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA replication; Nucleus; Reference proteome.
FT   CHAIN           1..213
FT                   /note="DNA replication complex GINS protein PSF2"
FT                   /id="PRO_0000194822"
FT   HELIX           5..7
FT                   /evidence="ECO:0007829|PDB:7PMK"
FT   STRAND          8..10
FT                   /evidence="ECO:0007829|PDB:7PMK"
FT   HELIX           13..21
FT                   /evidence="ECO:0007829|PDB:7PMK"
FT   STRAND          23..31
FT                   /evidence="ECO:0007829|PDB:7PMK"
FT   STRAND          53..55
FT                   /evidence="ECO:0007829|PDB:7PMK"
FT   HELIX           58..61
FT                   /evidence="ECO:0007829|PDB:7PMK"
FT   STRAND          69..72
FT                   /evidence="ECO:0007829|PDB:7PMK"
FT   HELIX           73..80
FT                   /evidence="ECO:0007829|PDB:7PMK"
FT   TURN            81..83
FT                   /evidence="ECO:0007829|PDB:7PMK"
FT   STRAND          84..87
FT                   /evidence="ECO:0007829|PDB:7PMK"
FT   HELIX           95..107
FT                   /evidence="ECO:0007829|PDB:7PMK"
FT   STRAND          109..111
FT                   /evidence="ECO:0007829|PDB:7PMK"
FT   HELIX           119..129
FT                   /evidence="ECO:0007829|PDB:7PMK"
FT   HELIX           138..160
FT                   /evidence="ECO:0007829|PDB:7PMK"
FT   HELIX           175..199
FT                   /evidence="ECO:0007829|PDB:7PMK"
SQ   SEQUENCE   213 AA;  25061 MW;  C8B52C0462A37A3A CRC64;
     MSLPAHLQQT FSPEEIQFIV ENEPIKIFPR ITTRQKIRGD DRGTGNHTRW QLITTDDKAL
     NNMVAMRSTE VVLWIALLLK QQSKCSIVAP QWLTTKELDR KIQYEKTHPD RFSELPWNWL
     VLARILFNKA KDDFHDPIHE LRGKIQDLRE IRQIKVLKGL KYLNESHLQL DNLSLLEINE
     LRPFITEIMD KLREIHTASL TAGTENDEEE FNI
//