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Protein

DNA replication complex GINS protein PSF2

Gene

PSF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as part of the GINS complex which plays an essential role in the initiation of DNA replication by binding to DNA replication origins and facilitating the assembly of the DNA replication machinery.1 Publication

GO - Biological processi

  1. DNA-dependent DNA replication Source: SGD
  2. double-strand break repair via break-induced replication Source: SGD
Complete GO annotation...

Keywords - Biological processi

DNA replication

Enzyme and pathway databases

BioCyciYEAST:G3O-31530-MONOMER.
ReactomeiREACT_255883. Unwinding of DNA.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication complex GINS protein PSF2
Alternative name(s):
Partner of Sld five 2
Gene namesi
Name:PSF2
Ordered Locus Names:YJL072C
ORF Names:HRF213, J1086
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJL072c.
SGDiS000003608. PSF2.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. DNA replication preinitiation complex Source: SGD
  2. GINS complex Source: SGD
  3. nuclear replication fork Source: SGD
  4. nucleus Source: SGD
  5. replication fork protection complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 213213DNA replication complex GINS protein PSF2PRO_0000194822Add
BLAST

Proteomic databases

MaxQBiP40359.
PaxDbiP40359.

Expressioni

Gene expression databases

GenevestigatoriP40359.

Interactioni

Subunit structurei

Component of the GINS complex which is a heterotetramer of SLD5, PSF1, PSF2 and PSF3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
PSF1Q124883EBI-25936,EBI-22066
PSF3Q121465EBI-25936,EBI-30392
SLD5Q034068EBI-25936,EBI-37437

Protein-protein interaction databases

BioGridi33683. 21 interactions.
DIPiDIP-3858N.
IntActiP40359. 6 interactions.
MINTiMINT-550028.
STRINGi4932.YJL072C.

Structurei

3D structure databases

ProteinModelPortaliP40359.
SMRiP40359. Positions 15-197.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GINS2/PSF2 family.Curated

Phylogenomic databases

eggNOGiCOG5093.
GeneTreeiENSGT00390000007838.
HOGENOMiHOG000246603.
InParanoidiP40359.
KOiK10733.
OMAiRNKHDST.
OrthoDBiEOG7XH72F.

Family and domain databases

InterProiIPR021151. GINS_complex.
IPR007257. GINS_Psf2.
IPR016906. GINS_Psf2_subgr.
[Graphical view]
PANTHERiPTHR12772. PTHR12772. 1 hit.
PfamiPF05916. Sld5. 1 hit.
[Graphical view]
PIRSFiPIRSF028998. GINS_Psf2_subgr. 1 hit.

Sequencei

Sequence statusi: Complete.

P40359-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLPAHLQQT FSPEEIQFIV ENEPIKIFPR ITTRQKIRGD DRGTGNHTRW
60 70 80 90 100
QLITTDDKAL NNMVAMRSTE VVLWIALLLK QQSKCSIVAP QWLTTKELDR
110 120 130 140 150
KIQYEKTHPD RFSELPWNWL VLARILFNKA KDDFHDPIHE LRGKIQDLRE
160 170 180 190 200
IRQIKVLKGL KYLNESHLQL DNLSLLEINE LRPFITEIMD KLREIHTASL
210
TAGTENDEEE FNI
Length:213
Mass (Da):25,061
Last modified:February 1, 1995 - v1
Checksum:iC8B52C0462A37A3A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X88851 Genomic DNA. Translation: CAA61311.1.
Z34288 Genomic DNA. Translation: CAA84050.1.
Z49347 Genomic DNA. Translation: CAA89364.1.
AY558248 Genomic DNA. Translation: AAS56574.1.
BK006943 Genomic DNA. Translation: DAA08727.1.
PIRiS50799.
RefSeqiNP_012463.1. NM_001181505.1.

Genome annotation databases

EnsemblFungiiYJL072C; YJL072C; YJL072C.
GeneIDi853373.
KEGGisce:YJL072C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X88851 Genomic DNA. Translation: CAA61311.1.
Z34288 Genomic DNA. Translation: CAA84050.1.
Z49347 Genomic DNA. Translation: CAA89364.1.
AY558248 Genomic DNA. Translation: AAS56574.1.
BK006943 Genomic DNA. Translation: DAA08727.1.
PIRiS50799.
RefSeqiNP_012463.1. NM_001181505.1.

3D structure databases

ProteinModelPortaliP40359.
SMRiP40359. Positions 15-197.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33683. 21 interactions.
DIPiDIP-3858N.
IntActiP40359. 6 interactions.
MINTiMINT-550028.
STRINGi4932.YJL072C.

Proteomic databases

MaxQBiP40359.
PaxDbiP40359.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL072C; YJL072C; YJL072C.
GeneIDi853373.
KEGGisce:YJL072C.

Organism-specific databases

CYGDiYJL072c.
SGDiS000003608. PSF2.

Phylogenomic databases

eggNOGiCOG5093.
GeneTreeiENSGT00390000007838.
HOGENOMiHOG000246603.
InParanoidiP40359.
KOiK10733.
OMAiRNKHDST.
OrthoDBiEOG7XH72F.

Enzyme and pathway databases

BioCyciYEAST:G3O-31530-MONOMER.
ReactomeiREACT_255883. Unwinding of DNA.

Miscellaneous databases

NextBioi973816.
PROiP40359.

Gene expression databases

GenevestigatoriP40359.

Family and domain databases

InterProiIPR021151. GINS_complex.
IPR007257. GINS_Psf2.
IPR016906. GINS_Psf2_subgr.
[Graphical view]
PANTHERiPTHR12772. PTHR12772. 1 hit.
PfamiPF05916. Sld5. 1 hit.
[Graphical view]
PIRSFiPIRSF028998. GINS_Psf2_subgr. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast."
    Takayama Y., Kamimura Y., Okawa M., Muramatsu S., Sugino A., Araki H.
    Genes Dev. 17:1153-1165(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, IDENTIFICATION IN THE GINS COMPLEX, SUBCELLULAR LOCATION.
  2. Sor F.J.
    Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Sequence of a 17.1 kb DNA fragment from chromosome X of Saccharomyces cerevisiae includes the mitochondrial ribosomal protein L8."
    Vandenbol M., Durand P., Dion C., Portetelle D., Hilger F.
    Yeast 11:57-60(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.

Entry informationi

Entry nameiPSF2_YEAST
AccessioniPrimary (citable) accession number: P40359
Secondary accession number(s): D6VWB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: January 7, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.