P40359 (PSF2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA replication complex GINS protein PSF2

Alternative name(s):
Partner of Sld five 2

Gene names

Name:	PSF2
Ordered Locus Names:	YJL072C
ORF Names:	HRF213, J1086

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Functions as part of the GINS complex which plays an essential role in the initiation of DNA replication by binding to DNA replication origins and facilitating the assembly of the DNA replication machinery. Ref.1
Subunit structure	Component of the GINS complex which is a heterotetramer of SLD5, PSF1, PSF2 and PSF3.
Subcellular location	Nucleus Ref.1.
Sequence similarities	Belongs to the GINS2/PSF2 family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA-dependent DNA replication Inferred from mutant phenotype PubMed 12768207. Source: SGD double-strand break repair via break-induced replication Inferred from mutant phenotype PubMed 20516198. Source: SGD
Cellular_component	GINS complex Inferred from physical interaction Ref.1PubMed 12768207. Source: SGD replication fork protection complex Inferred from direct assay PubMed 16531994. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 213	213	DNA replication complex GINS protein PSF2		PRO_0000194822

Sequences

	Sequence		Length	Mass (Da)
	P40359 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: C8B52C0462A37A3A	FASTA	213	25,061
	10 20 30 40 50 60 MSLPAHLQQT FSPEEIQFIV ENEPIKIFPR ITTRQKIRGD DRGTGNHTRW QLITTDDKAL 70 80 90 100 110 120 NNMVAMRSTE VVLWIALLLK QQSKCSIVAP QWLTTKELDR KIQYEKTHPD RFSELPWNWL 130 140 150 160 170 180 VLARILFNKA KDDFHDPIHE LRGKIQDLRE IRQIKVLKGL KYLNESHLQL DNLSLLEINE 190 200 210 LRPFITEIMD KLREIHTASL TAGTENDEEE FNI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast." Takayama Y., Kamimura Y., Okawa M., Muramatsu S., Sugino A., Araki H. Genes Dev. 17:1153-1165(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AND COMPOSITION OF THE GINS COMPLEX, SUBCELLULAR LOCATION.
[2]	Sor F.J. Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Sequence of a 17.1 kb DNA fragment from chromosome X of Saccharomyces cerevisiae includes the mitochondrial ribosomal protein L8." Vandenbol M., Durand P., Dion C., Portetelle D., Hilger F. Yeast 11:57-60(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X88851 Genomic DNA. Translation: CAA61311.1. Z34288 Genomic DNA. Translation: CAA84050.1. Z49347 Genomic DNA. Translation: CAA89364.1. AY558248 Genomic DNA. Translation: AAS56574.1. BK006943 Genomic DNA. Translation: DAA08727.1.
PIR	S50799.
RefSeq	NP_012463.1. NM_001181505.1.
3D structure databases
ProteinModelPortal	P40359.
SMR	P40359. Positions 15-197.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3858N.
IntAct	P40359. 4 interactions.
MINT	MINT-550028.
STRING	4932.YJL072C.
Proteomic databases
PaxDb	P40359.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL072C; YJL072C; YJL072C.
GeneID	853373.
KEGG	sce:YJL072C.
Organism-specific databases
CYGD	YJL072c.
SGD	S000003608. PSF2.
Phylogenomic databases
eggNOG	COG5093.
GeneTree	ENSGT00390000007838.
HOGENOM	HOG000246603.
KO	K10733.
OMA	MEINEMR.
OrthoDB	EOG4R274H.
Gene expression databases
Genevestigator	P40359.
GermOnline	YJL072C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR021151. GINS_complex. IPR007257. GINS_Psf2. IPR016906. GINS_Psf2_subgr. [Graphical view]
PANTHER	PTHR12772. PTHR12772. 1 hit.
Pfam	PF05916. Sld5. 1 hit. [Graphical view]
PIRSF	PIRSF028998. GINS_Psf2_subgr. 1 hit.
ProtoNet	Search...
Other
NextBio	973816.

Entry information

Entry name

PSF2_YEAST

Accession

Primary (citable) accession number: P40359
Secondary accession number(s): D6VWB1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program