Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

DnaJ-like chaperone JEM1

Gene

JEM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a DnaJ-like chaperone required for nuclear membrane fusion during mating.1 Publication

GO - Molecular functioni

  • chaperone binding Source: SGD
  • unfolded protein binding Source: SGD

GO - Biological processi

  • ER-associated ubiquitin-dependent protein catabolic process Source: SGD
  • karyogamy involved in conjugation with cellular fusion Source: SGD
  • protein folding in endoplasmic reticulum Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Enzyme and pathway databases

BioCyciYEAST:G3O-31531-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ-like chaperone JEM1
Alternative name(s):
DnaJ-like protein of the ER membrane 1
Gene namesi
Name:JEM1
Synonyms:KAR8
Ordered Locus Names:YJL073W
ORF Names:HRC558, J1083
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome X

Organism-specific databases

CYGDiYJL073w.
EuPathDBiFungiDB:YJL073W.
SGDiS000003609. JEM1.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: SGD
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • nuclear membrane Source: UniProtKB-SubCell
  • nuclear outer membrane-endoplasmic reticulum membrane network Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 645623DnaJ-like chaperone JEM1PRO_0000043353Add
BLAST

Proteomic databases

MaxQBiP40358.
PaxDbiP40358.

Interactioni

Subunit structurei

Interacts with MPS3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MPS3P470692EBI-25940,EBI-25811

Protein-protein interaction databases

BioGridi33682. 35 interactions.
IntActiP40358. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP40358.
SMRiP40358. Positions 79-109, 475-611.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini538 – 60871JPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi493 – 52533Gln-richAdd
BLAST

Sequence similaritiesi

Contains 1 J domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2214.
GeneTreeiENSGT00800000124896.
HOGENOMiHOG000113128.
InParanoidiP40358.
KOiK09523.
OMAiYHPDKQG.
OrthoDBiEOG7XM38G.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS50076. DNAJ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P40358-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILISGYCLL VYSVILPVLI SASKLCDLAE LQRLNKNLKV DTESLPKYQW
60 70 80 90 100
IAGQLEQNCM TADPASENMS DVIQLANQIY YKIGLIQLSN DQHLRAINTF
110 120 130 140 150
EKIVFNETYK GSFGKLAEKR LQELYVDFGM WDKVHQKDDQ YAKYLSLNET
160 170 180 190 200
IRNKISSKDV SVEEDISELL RITPYDVNVL STHIDVLFHK LAEEIDVSLA
210 220 230 240 250
AAIILDYETI LDKHLASLSI DTRLSIHYVI SVLQTFVLNS DASFNIRKCL
260 270 280 290 300
SIDMDYDKCK KLSLTISKLN KVNPSKRQIL DPATYAFENK KFRSWDRIIE
310 320 330 340 350
FYLKDKKPFI TPMKILNKDT NFKNNYFFLE EIIKQLIEDV QLSRPLAKNL
360 370 380 390 400
FEDPPITDGF VKPKSYYHTD YLVYIDSILC QASSMSPDVK RAKLAAPFCK
410 420 430 440 450
KSLRHSLTLE TWKHYQDAKS EQKPLPETVL SDVWNSNPHL LMYMVNSILN
460 470 480 490 500
KSRSKPHSQF KKQLYDQINK FFQDNGLSES TNPYVMKNFR LLQKQLQTYK
510 520 530 540 550
EHKHRNFNQQ YFQQQQQQQQ HQRHQAPPAA PNYDPKKDYY KILGVSPSAS
560 570 580 590 600
SKEIRKAYLN LTKKYHPDKI KANHNDKQES IHETMSQINE AYETLSDDDK
610 620 630 640
RKEYDLSRSN PRRNTFPQGP RQNNMFKNPG SGFPFGNGFK MNFGL
Length:645
Mass (Da):75,144
Last modified:December 6, 2005 - v3
Checksum:i43F219CD606F5D90
GO

Sequence cautioni

The sequence CAA61312.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA89365.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X88851 Genomic DNA. Translation: CAA61312.1. Different initiation.
Z49348 Genomic DNA. Translation: CAA89365.1. Different initiation.
Z34288 Genomic DNA. Translation: CAA84049.1.
BK006943 Genomic DNA. Translation: DAA08726.1.
PIRiS56849.
RefSeqiNP_012462.3. NM_001181506.3.

Genome annotation databases

EnsemblFungiiYJL073W; YJL073W; YJL073W.
GeneIDi853372.
KEGGisce:YJL073W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X88851 Genomic DNA. Translation: CAA61312.1. Different initiation.
Z49348 Genomic DNA. Translation: CAA89365.1. Different initiation.
Z34288 Genomic DNA. Translation: CAA84049.1.
BK006943 Genomic DNA. Translation: DAA08726.1.
PIRiS56849.
RefSeqiNP_012462.3. NM_001181506.3.

3D structure databases

ProteinModelPortaliP40358.
SMRiP40358. Positions 79-109, 475-611.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33682. 35 interactions.
IntActiP40358. 1 interaction.

Proteomic databases

MaxQBiP40358.
PaxDbiP40358.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL073W; YJL073W; YJL073W.
GeneIDi853372.
KEGGisce:YJL073W.

Organism-specific databases

CYGDiYJL073w.
EuPathDBiFungiDB:YJL073W.
SGDiS000003609. JEM1.

Phylogenomic databases

eggNOGiCOG2214.
GeneTreeiENSGT00800000124896.
HOGENOMiHOG000113128.
InParanoidiP40358.
KOiK09523.
OMAiYHPDKQG.
OrthoDBiEOG7XM38G.

Enzyme and pathway databases

BioCyciYEAST:G3O-31531-MONOMER.

Miscellaneous databases

NextBioi973813.
PROiP40358.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS50076. DNAJ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast JEM1p is a DnaJ-like protein of the endoplasmic reticulum membrane required for nuclear fusion."
    Nishikawa S., Endo T.
    J. Biol. Chem. 272:12889-12892(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION OF INITIATION SITE, FUNCTION, SUBCELLULAR LOCATION.
  2. Sor F.J.
    Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Sequence of a 17.1 kb DNA fragment from chromosome X of Saccharomyces cerevisiae includes the mitochondrial ribosomal protein L8."
    Vandenbol M., Durand P., Dion C., Portetelle D., Hilger F.
    Yeast 11:57-60(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 135-645.
    Strain: ATCC 204508 / S288c.
  6. "Nep98p is a component of the yeast spindle pole body and essential for nuclear division and fusion."
    Nishikawa S., Terazawa Y., Nakayama T., Hirata A., Makio T., Endo T.
    J. Biol. Chem. 278:9938-9943(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MPS3.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Localization of proteins that are coordinately expressed with Cln2 during the cell cycle."
    Sundin B.A., Chiu C.-H., Riffle M., Davis T.N., Muller E.G.D.
    Yeast 21:793-800(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiJEM1_YEAST
AccessioniPrimary (citable) accession number: P40358
Secondary accession number(s): D6VWB0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: December 6, 2005
Last modified: July 22, 2015
This is version 130 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3180 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.