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P40358 (JEM1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DnaJ-like chaperone JEM1
Alternative name(s):
DnaJ-like protein of the ER membrane 1
Gene names
Name:JEM1
Synonyms:KAR8
Ordered Locus Names:YJL073W
ORF Names:HRC558, J1083
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length645 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a DnaJ-like chaperone required for nuclear membrane fusion during mating. Ref.1

Subunit structure

Interacts with MPS3. Ref.6

Subcellular location

Endoplasmic reticulum membrane. Nucleus membrane Ref.1 Ref.8.

Miscellaneous

Present with 3180 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 J domain.

Sequence caution

The sequence CAA61312.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAA89365.1 differs from that shown. Reason: Erroneous initiation.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MPS3P470692EBI-25940,EBI-25811

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 645623DnaJ-like chaperone JEM1
PRO_0000043353

Regions

Domain538 – 60871J
Compositional bias493 – 52533Gln-rich

Sequences

Sequence LengthMass (Da)Tools
P40358 [UniParc].

Last modified December 6, 2005. Version 3.
Checksum: 43F219CD606F5D90

FASTA64575,144
        10         20         30         40         50         60 
MILISGYCLL VYSVILPVLI SASKLCDLAE LQRLNKNLKV DTESLPKYQW IAGQLEQNCM 

        70         80         90        100        110        120 
TADPASENMS DVIQLANQIY YKIGLIQLSN DQHLRAINTF EKIVFNETYK GSFGKLAEKR 

       130        140        150        160        170        180 
LQELYVDFGM WDKVHQKDDQ YAKYLSLNET IRNKISSKDV SVEEDISELL RITPYDVNVL 

       190        200        210        220        230        240 
STHIDVLFHK LAEEIDVSLA AAIILDYETI LDKHLASLSI DTRLSIHYVI SVLQTFVLNS 

       250        260        270        280        290        300 
DASFNIRKCL SIDMDYDKCK KLSLTISKLN KVNPSKRQIL DPATYAFENK KFRSWDRIIE 

       310        320        330        340        350        360 
FYLKDKKPFI TPMKILNKDT NFKNNYFFLE EIIKQLIEDV QLSRPLAKNL FEDPPITDGF 

       370        380        390        400        410        420 
VKPKSYYHTD YLVYIDSILC QASSMSPDVK RAKLAAPFCK KSLRHSLTLE TWKHYQDAKS 

       430        440        450        460        470        480 
EQKPLPETVL SDVWNSNPHL LMYMVNSILN KSRSKPHSQF KKQLYDQINK FFQDNGLSES 

       490        500        510        520        530        540 
TNPYVMKNFR LLQKQLQTYK EHKHRNFNQQ YFQQQQQQQQ HQRHQAPPAA PNYDPKKDYY 

       550        560        570        580        590        600 
KILGVSPSAS SKEIRKAYLN LTKKYHPDKI KANHNDKQES IHETMSQINE AYETLSDDDK 

       610        620        630        640 
RKEYDLSRSN PRRNTFPQGP RQNNMFKNPG SGFPFGNGFK MNFGL 

« Hide

References

« Hide 'large scale' references
[1]"The yeast JEM1p is a DnaJ-like protein of the endoplasmic reticulum membrane required for nuclear fusion."
Nishikawa S., Endo T.
J. Biol. Chem. 272:12889-12892(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION OF INITIATION SITE, FUNCTION, SUBCELLULAR LOCATION.
[2]Sor F.J.
Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Sequence of a 17.1 kb DNA fragment from chromosome X of Saccharomyces cerevisiae includes the mitochondrial ribosomal protein L8."
Vandenbol M., Durand P., Dion C., Portetelle D., Hilger F.
Yeast 11:57-60(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 135-645.
Strain: ATCC 204508 / S288c.
[6]"Nep98p is a component of the yeast spindle pole body and essential for nuclear division and fusion."
Nishikawa S., Terazawa Y., Nakayama T., Hirata A., Makio T., Endo T.
J. Biol. Chem. 278:9938-9943(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MPS3.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Localization of proteins that are coordinately expressed with Cln2 during the cell cycle."
Sundin B.A., Chiu C.-H., Riffle M., Davis T.N., Muller E.G.D.
Yeast 21:793-800(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X88851 Genomic DNA. Translation: CAA61312.1. Different initiation.
Z49348 Genomic DNA. Translation: CAA89365.1. Different initiation.
Z34288 Genomic DNA. Translation: CAA84049.1.
BK006943 Genomic DNA. Translation: DAA08726.1.
PIRS56849.
RefSeqNP_012462.3. NM_001181506.3.

3D structure databases

ProteinModelPortalP40358.
SMRP40358. Positions 475-611.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33682. 36 interactions.
IntActP40358. 1 interaction.
STRING4932.YJL073W.

Proteomic databases

PaxDbP40358.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL073W; YJL073W; YJL073W.
GeneID853372.
KEGGsce:YJL073W.

Organism-specific databases

CYGDYJL073w.
SGDS000003609. JEM1.

Phylogenomic databases

eggNOGCOG2214.
GeneTreeENSGT00730000113815.
HOGENOMHOG000113128.
KOK09523.
OMAYHPDKQG.
OrthoDBEOG7XM38G.

Enzyme and pathway databases

BioCycYEAST:G3O-31531-MONOMER.

Gene expression databases

GenevestigatorP40358.

Family and domain databases

Gene3D1.10.287.110. 1 hit.
InterProIPR001623. DnaJ_domain.
[Graphical view]
PfamPF00226. DnaJ. 1 hit.
[Graphical view]
PRINTSPR00625. JDOMAIN.
SMARTSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMSSF46565. SSF46565. 1 hit.
PROSITEPS50076. DNAJ_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973813.

Entry information

Entry nameJEM1_YEAST
AccessionPrimary (citable) accession number: P40358
Secondary accession number(s): D6VWB0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: December 6, 2005
Last modified: March 19, 2014
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families