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P40358

- JEM1_YEAST

UniProt

P40358 - JEM1_YEAST

Protein

DnaJ-like chaperone JEM1

Gene

JEM1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 3 (06 Dec 2005)
      Previous versions | rss
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    Functioni

    Acts as a DnaJ-like chaperone required for nuclear membrane fusion during mating.1 Publication

    GO - Molecular functioni

    1. chaperone binding Source: SGD
    2. protein binding Source: IntAct
    3. unfolded protein binding Source: SGD

    GO - Biological processi

    1. ER-associated ubiquitin-dependent protein catabolic process Source: SGD
    2. karyogamy involved in conjugation with cellular fusion Source: SGD
    3. protein folding in endoplasmic reticulum Source: SGD

    Keywords - Molecular functioni

    Chaperone

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31531-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DnaJ-like chaperone JEM1
    Alternative name(s):
    DnaJ-like protein of the ER membrane 1
    Gene namesi
    Name:JEM1
    Synonyms:KAR8
    Ordered Locus Names:YJL073W
    ORF Names:HRC558, J1083
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJL073w.
    SGDiS000003609. JEM1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: SGD
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. nuclear membrane Source: UniProtKB-SubCell
    4. nuclear outer membrane-endoplasmic reticulum membrane network Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Sequence AnalysisAdd
    BLAST
    Chaini23 – 645623DnaJ-like chaperone JEM1PRO_0000043353Add
    BLAST

    Proteomic databases

    MaxQBiP40358.
    PaxDbiP40358.

    Expressioni

    Gene expression databases

    GenevestigatoriP40358.

    Interactioni

    Subunit structurei

    Interacts with MPS3.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MPS3P470692EBI-25940,EBI-25811

    Protein-protein interaction databases

    BioGridi33682. 36 interactions.
    IntActiP40358. 1 interaction.
    STRINGi4932.YJL073W.

    Structurei

    3D structure databases

    ProteinModelPortaliP40358.
    SMRiP40358. Positions 79-109, 475-611.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini538 – 60871JPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi493 – 52533Gln-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 J domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2214.
    GeneTreeiENSGT00730000113815.
    HOGENOMiHOG000113128.
    KOiK09523.
    OMAiYHPDKQG.
    OrthoDBiEOG7XM38G.

    Family and domain databases

    Gene3Di1.10.287.110. 1 hit.
    InterProiIPR001623. DnaJ_domain.
    [Graphical view]
    PfamiPF00226. DnaJ. 1 hit.
    [Graphical view]
    PRINTSiPR00625. JDOMAIN.
    SMARTiSM00271. DnaJ. 1 hit.
    [Graphical view]
    SUPFAMiSSF46565. SSF46565. 1 hit.
    PROSITEiPS50076. DNAJ_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P40358-1 [UniParc]FASTAAdd to Basket

    « Hide

    MILISGYCLL VYSVILPVLI SASKLCDLAE LQRLNKNLKV DTESLPKYQW    50
    IAGQLEQNCM TADPASENMS DVIQLANQIY YKIGLIQLSN DQHLRAINTF 100
    EKIVFNETYK GSFGKLAEKR LQELYVDFGM WDKVHQKDDQ YAKYLSLNET 150
    IRNKISSKDV SVEEDISELL RITPYDVNVL STHIDVLFHK LAEEIDVSLA 200
    AAIILDYETI LDKHLASLSI DTRLSIHYVI SVLQTFVLNS DASFNIRKCL 250
    SIDMDYDKCK KLSLTISKLN KVNPSKRQIL DPATYAFENK KFRSWDRIIE 300
    FYLKDKKPFI TPMKILNKDT NFKNNYFFLE EIIKQLIEDV QLSRPLAKNL 350
    FEDPPITDGF VKPKSYYHTD YLVYIDSILC QASSMSPDVK RAKLAAPFCK 400
    KSLRHSLTLE TWKHYQDAKS EQKPLPETVL SDVWNSNPHL LMYMVNSILN 450
    KSRSKPHSQF KKQLYDQINK FFQDNGLSES TNPYVMKNFR LLQKQLQTYK 500
    EHKHRNFNQQ YFQQQQQQQQ HQRHQAPPAA PNYDPKKDYY KILGVSPSAS 550
    SKEIRKAYLN LTKKYHPDKI KANHNDKQES IHETMSQINE AYETLSDDDK 600
    RKEYDLSRSN PRRNTFPQGP RQNNMFKNPG SGFPFGNGFK MNFGL 645
    Length:645
    Mass (Da):75,144
    Last modified:December 6, 2005 - v3
    Checksum:i43F219CD606F5D90
    GO

    Sequence cautioni

    The sequence CAA61312.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA89365.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X88851 Genomic DNA. Translation: CAA61312.1. Different initiation.
    Z49348 Genomic DNA. Translation: CAA89365.1. Different initiation.
    Z34288 Genomic DNA. Translation: CAA84049.1.
    BK006943 Genomic DNA. Translation: DAA08726.1.
    PIRiS56849.
    RefSeqiNP_012462.3. NM_001181506.3.

    Genome annotation databases

    EnsemblFungiiYJL073W; YJL073W; YJL073W.
    GeneIDi853372.
    KEGGisce:YJL073W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X88851 Genomic DNA. Translation: CAA61312.1 . Different initiation.
    Z49348 Genomic DNA. Translation: CAA89365.1 . Different initiation.
    Z34288 Genomic DNA. Translation: CAA84049.1 .
    BK006943 Genomic DNA. Translation: DAA08726.1 .
    PIRi S56849.
    RefSeqi NP_012462.3. NM_001181506.3.

    3D structure databases

    ProteinModelPortali P40358.
    SMRi P40358. Positions 79-109, 475-611.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33682. 36 interactions.
    IntActi P40358. 1 interaction.
    STRINGi 4932.YJL073W.

    Proteomic databases

    MaxQBi P40358.
    PaxDbi P40358.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJL073W ; YJL073W ; YJL073W .
    GeneIDi 853372.
    KEGGi sce:YJL073W.

    Organism-specific databases

    CYGDi YJL073w.
    SGDi S000003609. JEM1.

    Phylogenomic databases

    eggNOGi COG2214.
    GeneTreei ENSGT00730000113815.
    HOGENOMi HOG000113128.
    KOi K09523.
    OMAi YHPDKQG.
    OrthoDBi EOG7XM38G.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31531-MONOMER.

    Miscellaneous databases

    NextBioi 973813.

    Gene expression databases

    Genevestigatori P40358.

    Family and domain databases

    Gene3Di 1.10.287.110. 1 hit.
    InterProi IPR001623. DnaJ_domain.
    [Graphical view ]
    Pfami PF00226. DnaJ. 1 hit.
    [Graphical view ]
    PRINTSi PR00625. JDOMAIN.
    SMARTi SM00271. DnaJ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46565. SSF46565. 1 hit.
    PROSITEi PS50076. DNAJ_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The yeast JEM1p is a DnaJ-like protein of the endoplasmic reticulum membrane required for nuclear fusion."
      Nishikawa S., Endo T.
      J. Biol. Chem. 272:12889-12892(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION OF INITIATION SITE, FUNCTION, SUBCELLULAR LOCATION.
    2. Sor F.J.
      Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Sequence of a 17.1 kb DNA fragment from chromosome X of Saccharomyces cerevisiae includes the mitochondrial ribosomal protein L8."
      Vandenbol M., Durand P., Dion C., Portetelle D., Hilger F.
      Yeast 11:57-60(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 135-645.
      Strain: ATCC 204508 / S288c.
    6. "Nep98p is a component of the yeast spindle pole body and essential for nuclear division and fusion."
      Nishikawa S., Terazawa Y., Nakayama T., Hirata A., Makio T., Endo T.
      J. Biol. Chem. 278:9938-9943(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MPS3.
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    8. "Localization of proteins that are coordinately expressed with Cln2 during the cell cycle."
      Sundin B.A., Chiu C.-H., Riffle M., Davis T.N., Muller E.G.D.
      Yeast 21:793-800(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiJEM1_YEAST
    AccessioniPrimary (citable) accession number: P40358
    Secondary accession number(s): D6VWB0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: December 6, 2005
    Last modified: October 1, 2014
    This is version 122 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3180 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3