ID   NTA1_YEAST              Reviewed;         457 AA.
AC   P40354; D6VWN3;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   27-MAR-2024, entry version 160.
DE   RecName: Full=Protein N-terminal amidase;
DE            Short=NT-amidase;
DE            EC=3.5.1.-;
GN   Name=NTA1; OrderedLocusNames=YJR062C; ORFNames=J1742;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7744855; DOI=10.1074/jbc.270.20.12065;
RA   Baker R.T., Varshavsky A.;
RT   "Yeast N-terminal amidase. A new enzyme and component of the N-end rule
RT   pathway.";
RL   J. Biol. Chem. 270:12065-12074(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8840504;
RX   DOI=10.1002/(sici)1097-0061(199607)12:9<869::aid-yea964>3.0.co;2-1;
RA   Huang M.-E., Manus V., Chuat J.-C., Galibert F.;
RT   "Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading
RT   frames and a gene cluster with a counterpart on chromosome XI.";
RL   Yeast 12:869-875(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA   Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA   Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA   Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA   Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA   Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA   Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA   Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA   Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA   Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA   To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA   von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT   "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL   EMBO J. 15:2031-2049(1996).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [5]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- FUNCTION: Deamidates N-terminal Asn and Gln. Component of a targeting
CC       complex in the N-end rule pathway.
CC   -!- MISCELLANEOUS: Present with 1640 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the carbon-nitrogen hydrolase superfamily.
CC       {ECO:0000305}.
CC   -!- CAUTION: It is uncertain whether Met-1 or Met-11 is the initiator.
CC       {ECO:0000305}.
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DR   EMBL; L35564; AAB59320.1; -; Genomic_DNA.
DR   EMBL; Z49562; CAA89590.1; -; Genomic_DNA.
DR   EMBL; L47993; AAB39288.1; -; Genomic_DNA.
DR   EMBL; BK006943; DAA08849.1; -; Genomic_DNA.
DR   PIR; S47938; S47938.
DR   RefSeq; NP_012596.3; NM_001181720.3.
DR   AlphaFoldDB; P40354; -.
DR   SMR; P40354; -.
DR   BioGRID; 33819; 50.
DR   DIP; DIP-6478N; -.
DR   IntAct; P40354; 23.
DR   STRING; 4932.YJR062C; -.
DR   iPTMnet; P40354; -.
DR   MaxQB; P40354; -.
DR   PaxDb; 4932-YJR062C; -.
DR   PeptideAtlas; P40354; -.
DR   EnsemblFungi; YJR062C_mRNA; YJR062C; YJR062C.
DR   GeneID; 853525; -.
DR   KEGG; sce:YJR062C; -.
DR   AGR; SGD:S000003823; -.
DR   SGD; S000003823; NTA1.
DR   VEuPathDB; FungiDB:YJR062C; -.
DR   eggNOG; ENOG502QVBD; Eukaryota.
DR   HOGENOM; CLU_009854_1_1_1; -.
DR   InParanoid; P40354; -.
DR   OMA; VKILCWD; -.
DR   OrthoDB; 297364at2759; -.
DR   BioCyc; YEAST:G3O-31695-MONOMER; -.
DR   BRENDA; 3.5.1.121; 984.
DR   BRENDA; 3.5.1.122; 984.
DR   BioGRID-ORCS; 853525; 0 hits in 10 CRISPR screens.
DR   PRO; PR:P40354; -.
DR   Proteomes; UP000002311; Chromosome X.
DR   RNAct; P40354; Protein.
DR   GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR   GO; GO:0008418; F:protein-N-terminal asparagine amidohydrolase activity; IDA:SGD.
DR   GO; GO:0070773; F:protein-N-terminal glutamine amidohydrolase activity; IDA:SGD.
DR   GO; GO:0031365; P:N-terminal protein amino acid modification; IMP:SGD.
DR   GO; GO:0030163; P:protein catabolic process; IMP:SGD.
DR   CDD; cd07566; ScNTA1_like; 1.
DR   Gene3D; 3.60.110.10; Carbon-nitrogen hydrolase; 1.
DR   InterPro; IPR003010; C-N_Hydrolase.
DR   InterPro; IPR036526; C-N_Hydrolase_sf.
DR   InterPro; IPR039703; Nta1.
DR   PANTHER; PTHR11750; PROTEIN N-TERMINAL AMIDASE; 1.
DR   PANTHER; PTHR11750:SF26; PROTEIN N-TERMINAL AMIDASE; 1.
DR   Pfam; PF00795; CN_hydrolase; 1.
DR   SUPFAM; SSF56317; Carbon-nitrogen hydrolase; 1.
DR   PROSITE; PS50263; CN_HYDROLASE; 1.
PE   1: Evidence at protein level;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..457
FT                   /note="Protein N-terminal amidase"
FT                   /id="PRO_0000204068"
FT   DOMAIN          19..453
FT                   /note="CN hydrolase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT   ACT_SITE        63
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT   ACT_SITE        136
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT   ACT_SITE        187
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
SQ   SEQUENCE   457 AA;  51897 MW;  E6259FBBFB25A889 CRC64;
     MLIDAIHGAK MSTKLLVSLK VLVIQLNPQI GQVDQTIKRT WSILDKVTKS ATYVKPDIIL
     FPEFALTGYS FHARKDILPY VTKKDEGPSF ELAKSISEKF QCYTIIGYPE DDDEQKLYNS
     ALVVNPQGEQ IFNYRKTFLY DTEMNWDCEE NPEGFQTFPM DFSKCAKLSN EDSYNRDVTL
     KASIGICMDL SPYKFMAPFN HFEFSSFCVD NNVELILCPM AWLNSTSITD KQTLHNNSLL
     EAAKNKIAFA LKEQGLPLAG SQGIYQLKIG DSQRTPRVPS DDSTSEYKDM DEPDMSNVNY
     WILRFFPFLY FKSRINWFKN SSLIESILGK TRMPLDHEYY KDGKHKEDTI DLLDSEEVIK
     DTVLEKTFLG TSLGQPWKFQ GKNAILVLAN RCGTEDGTTI FAGSSGIYKF NGKKPKGSQD
     DDESSLDSLN ESVELLGNLG KGLEGAILRE VQFEVFR
//