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Protein

Protein N-terminal amidase

Gene

NTA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Deamidates N-terminal Asn and Gln. Component of a targeting complex in the N-end rule pathway.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei63Proton acceptorPROSITE-ProRule annotation1
Active sitei136Proton donorPROSITE-ProRule annotation1
Active sitei187NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

  • protein-N-terminal asparagine amidohydrolase activity Source: SGD
  • protein-N-terminal glutamine amidohydrolase activity Source: SGD

GO - Biological processi

  • cellular protein modification process Source: SGD
  • nitrogen compound metabolic process Source: InterPro
  • protein catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciYEAST:G3O-31695-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein N-terminal amidase (EC:3.5.1.-)
Short name:
NT-amidase
Gene namesi
Name:NTA1
Ordered Locus Names:YJR062C
ORF Names:J1742
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR062C.
SGDiS000003823. NTA1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002040681 – 457Protein N-terminal amidaseAdd BLAST457

Proteomic databases

MaxQBiP40354.
PRIDEiP40354.

PTM databases

iPTMnetiP40354.

Interactioni

Protein-protein interaction databases

BioGridi33819. 22 interactors.
DIPiDIP-6478N.
IntActiP40354. 8 interactors.
MINTiMINT-673827.

Structurei

3D structure databases

ProteinModelPortaliP40354.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 319CN hydrolasePROSITE-ProRule annotationAdd BLAST301

Sequence similaritiesi

Contains 1 CN hydrolase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000174480.
InParanoidiP40354.
KOiK14663.
OMAiGICMDLS.
OrthoDBiEOG092C4BA1.

Family and domain databases

Gene3Di3.60.110.10. 2 hits.
InterProiIPR003010. C-N_Hydrolase.
[Graphical view]
PfamiPF00795. CN_hydrolase. 1 hit.
[Graphical view]
SUPFAMiSSF56317. SSF56317. 1 hit.
PROSITEiPS50263. CN_HYDROLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40354-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLIDAIHGAK MSTKLLVSLK VLVIQLNPQI GQVDQTIKRT WSILDKVTKS
60 70 80 90 100
ATYVKPDIIL FPEFALTGYS FHARKDILPY VTKKDEGPSF ELAKSISEKF
110 120 130 140 150
QCYTIIGYPE DDDEQKLYNS ALVVNPQGEQ IFNYRKTFLY DTEMNWDCEE
160 170 180 190 200
NPEGFQTFPM DFSKCAKLSN EDSYNRDVTL KASIGICMDL SPYKFMAPFN
210 220 230 240 250
HFEFSSFCVD NNVELILCPM AWLNSTSITD KQTLHNNSLL EAAKNKIAFA
260 270 280 290 300
LKEQGLPLAG SQGIYQLKIG DSQRTPRVPS DDSTSEYKDM DEPDMSNVNY
310 320 330 340 350
WILRFFPFLY FKSRINWFKN SSLIESILGK TRMPLDHEYY KDGKHKEDTI
360 370 380 390 400
DLLDSEEVIK DTVLEKTFLG TSLGQPWKFQ GKNAILVLAN RCGTEDGTTI
410 420 430 440 450
FAGSSGIYKF NGKKPKGSQD DDESSLDSLN ESVELLGNLG KGLEGAILRE

VQFEVFR
Length:457
Mass (Da):51,897
Last modified:February 1, 1995 - v1
Checksum:iE6259FBBFB25A889
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35564 Genomic DNA. Translation: AAB59320.1.
Z49562 Genomic DNA. Translation: CAA89590.1.
L47993 Genomic DNA. Translation: AAB39288.1.
BK006943 Genomic DNA. Translation: DAA08849.1.
PIRiS47938.
RefSeqiNP_012596.3. NM_001181720.3.

Genome annotation databases

EnsemblFungiiYJR062C; YJR062C; YJR062C.
GeneIDi853525.
KEGGisce:YJR062C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35564 Genomic DNA. Translation: AAB59320.1.
Z49562 Genomic DNA. Translation: CAA89590.1.
L47993 Genomic DNA. Translation: AAB39288.1.
BK006943 Genomic DNA. Translation: DAA08849.1.
PIRiS47938.
RefSeqiNP_012596.3. NM_001181720.3.

3D structure databases

ProteinModelPortaliP40354.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33819. 22 interactors.
DIPiDIP-6478N.
IntActiP40354. 8 interactors.
MINTiMINT-673827.

PTM databases

iPTMnetiP40354.

Proteomic databases

MaxQBiP40354.
PRIDEiP40354.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR062C; YJR062C; YJR062C.
GeneIDi853525.
KEGGisce:YJR062C.

Organism-specific databases

EuPathDBiFungiDB:YJR062C.
SGDiS000003823. NTA1.

Phylogenomic databases

HOGENOMiHOG000174480.
InParanoidiP40354.
KOiK14663.
OMAiGICMDLS.
OrthoDBiEOG092C4BA1.

Enzyme and pathway databases

BioCyciYEAST:G3O-31695-MONOMER.

Miscellaneous databases

PROiP40354.

Family and domain databases

Gene3Di3.60.110.10. 2 hits.
InterProiIPR003010. C-N_Hydrolase.
[Graphical view]
PfamiPF00795. CN_hydrolase. 1 hit.
[Graphical view]
SUPFAMiSSF56317. SSF56317. 1 hit.
PROSITEiPS50263. CN_HYDROLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNTA1_YEAST
AccessioniPrimary (citable) accession number: P40354
Secondary accession number(s): D6VWN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1640 molecules/cell in log phase SD medium.1 Publication

Caution

It is uncertain whether Met-1 or Met-11 is the initiator.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.