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P40354 (NTA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein N-terminal amidase

Short name=NT-amidase
EC=3.5.1.-
Gene names
Name:NTA1
Ordered Locus Names:YJR062C
ORF Names:J1742
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length457 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Deamidates N-terminal Asn and Gln. Component of a targeting complex in the N-end rule pathway.

Miscellaneous

Present with 1640 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the carbon-nitrogen hydrolase superfamily.

Contains 1 CN hydrolase domain.

Caution

It is uncertain whether Met-1 or Met-11 is the initiator.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 457457Protein N-terminal amidase
PRO_0000204068

Regions

Domain19 – 319301CN hydrolase

Sites

Active site631Proton acceptor Potential
Active site1361 Potential
Active site1871Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
P40354 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: E6259FBBFB25A889

FASTA45751,897
        10         20         30         40         50         60 
MLIDAIHGAK MSTKLLVSLK VLVIQLNPQI GQVDQTIKRT WSILDKVTKS ATYVKPDIIL 

        70         80         90        100        110        120 
FPEFALTGYS FHARKDILPY VTKKDEGPSF ELAKSISEKF QCYTIIGYPE DDDEQKLYNS 

       130        140        150        160        170        180 
ALVVNPQGEQ IFNYRKTFLY DTEMNWDCEE NPEGFQTFPM DFSKCAKLSN EDSYNRDVTL 

       190        200        210        220        230        240 
KASIGICMDL SPYKFMAPFN HFEFSSFCVD NNVELILCPM AWLNSTSITD KQTLHNNSLL 

       250        260        270        280        290        300 
EAAKNKIAFA LKEQGLPLAG SQGIYQLKIG DSQRTPRVPS DDSTSEYKDM DEPDMSNVNY 

       310        320        330        340        350        360 
WILRFFPFLY FKSRINWFKN SSLIESILGK TRMPLDHEYY KDGKHKEDTI DLLDSEEVIK 

       370        380        390        400        410        420 
DTVLEKTFLG TSLGQPWKFQ GKNAILVLAN RCGTEDGTTI FAGSSGIYKF NGKKPKGSQD 

       430        440        450 
DDESSLDSLN ESVELLGNLG KGLEGAILRE VQFEVFR 

« Hide

References

« Hide 'large scale' references
[1]"Yeast N-terminal amidase. A new enzyme and component of the N-end rule pathway."
Baker R.T., Varshavsky A.
J. Biol. Chem. 270:12065-12074(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI."
Huang M.-E., Manus V., Chuat J.-C., Galibert F.
Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L35564 Genomic DNA. Translation: AAB59320.1.
Z49562 Genomic DNA. Translation: CAA89590.1.
L47993 Genomic DNA. Translation: AAB39288.1.
BK006943 Genomic DNA. Translation: DAA08849.1.
PIRS47938.
RefSeqNP_012596.3. NM_001181720.3.

3D structure databases

ProteinModelPortalP40354.
SMRP40354. Positions 19-164.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33819. 22 interactions.
DIPDIP-6478N.
IntActP40354. 8 interactions.
MINTMINT-673827.
STRING4932.YJR062C.

Proteomic databases

PaxDbP40354.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJR062C; YJR062C; YJR062C.
GeneID853525.
KEGGsce:YJR062C.

Organism-specific databases

CYGDYJR062c.
SGDS000003823. NTA1.

Phylogenomic databases

eggNOGCOG0388.
HOGENOMHOG000174480.
KOK14663.
OMAGICMDLS.
OrthoDBEOG7ZSJ4C.

Enzyme and pathway databases

BioCycYEAST:G3O-31695-MONOMER.

Gene expression databases

GenevestigatorP40354.

Family and domain databases

Gene3D3.60.110.10. 2 hits.
InterProIPR003010. C-N_Hydrolase.
[Graphical view]
PfamPF00795. CN_hydrolase. 1 hit.
[Graphical view]
SUPFAMSSF56317. SSF56317. 1 hit.
PROSITEPS50263. CN_HYDROLASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974212.

Entry information

Entry nameNTA1_YEAST
AccessionPrimary (citable) accession number: P40354
Secondary accession number(s): D6VWN3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 19, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families