P40354 (NTA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein N-terminal amidase
Short name=NT-amidase
EC=3.5.1.-

Gene names

Name:	NTA1
Ordered Locus Names:	YJR062C
ORF Names:	J1742

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Deamidates N-terminal Asn and Gln. Component of a targeting complex in the N-end rule pathway.
Miscellaneous	Present with 1640 molecules/cell in log phase SD medium.
Sequence similarities	Contains 1 CN hydrolase domain.
Caution	It is uncertain whether Met-1 or Met-11 is the initiator.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular protein modification process Inferred from mutant phenotype Ref.1. Source: SGD nitrogen compound metabolic process Inferred from electronic annotation. Source: InterPro protein catabolic process Inferred from mutant phenotype Ref.1. Source: SGD
Cellular_component	mitochondrion Inferred from direct assay PubMed 14562095. Source: SGD
Molecular_function	protein-N-terminal asparagine amidohydrolase activity Inferred from direct assay PubMed 23218487Ref.1. Source: SGD protein-N-terminal glutamine amidohydrolase activity Inferred from direct assay PubMed 23218487. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 457	457	Protein N-terminal amidase		PRO_0000204068
Regions
	Domain	19 – 319	301	CN hydrolase
Sites
	Active site	63	1	Proton acceptor Potential
	Active site	136	1	Potential
	Active site	187	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P40354 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: E6259FBBFB25A889	FASTA	457	51,897
	10 20 30 40 50 60 MLIDAIHGAK MSTKLLVSLK VLVIQLNPQI GQVDQTIKRT WSILDKVTKS ATYVKPDIIL 70 80 90 100 110 120 FPEFALTGYS FHARKDILPY VTKKDEGPSF ELAKSISEKF QCYTIIGYPE DDDEQKLYNS 130 140 150 160 170 180 ALVVNPQGEQ IFNYRKTFLY DTEMNWDCEE NPEGFQTFPM DFSKCAKLSN EDSYNRDVTL 190 200 210 220 230 240 KASIGICMDL SPYKFMAPFN HFEFSSFCVD NNVELILCPM AWLNSTSITD KQTLHNNSLL 250 260 270 280 290 300 EAAKNKIAFA LKEQGLPLAG SQGIYQLKIG DSQRTPRVPS DDSTSEYKDM DEPDMSNVNY 310 320 330 340 350 360 WILRFFPFLY FKSRINWFKN SSLIESILGK TRMPLDHEYY KDGKHKEDTI DLLDSEEVIK 370 380 390 400 410 420 DTVLEKTFLG TSLGQPWKFQ GKNAILVLAN RCGTEDGTTI FAGSSGIYKF NGKKPKGSQD 430 440 450 DDESSLDSLN ESVELLGNLG KGLEGAILRE VQFEVFR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast N-terminal amidase. A new enzyme and component of the N-end rule pathway." Baker R.T., Varshavsky A. J. Biol. Chem. 270:12065-12074(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI." Huang M.-E., Manus V., Chuat J.-C., Galibert F. Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L35564 Genomic DNA. Translation: AAB59320.1. Z49562 Genomic DNA. Translation: CAA89590.1. L47993 Genomic DNA. Translation: AAB39288.1. BK006943 Genomic DNA. Translation: DAA08849.1.
PIR	S47938.
RefSeq	NP_012596.3. NM_001181720.3.
3D structure databases
ProteinModelPortal	P40354.
SMR	P40354. Positions 19-164.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6478N.
IntAct	P40354. 15 interactions.
MINT	MINT-673827.
STRING	4932.YJR062C.
Proteomic databases
PaxDb	P40354.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR062C; YJR062C; YJR062C.
GeneID	853525.
KEGG	sce:YJR062C. sce:YJR065C.
Organism-specific databases
CYGD	YJR062c.
SGD	S000003823. NTA1.
Phylogenomic databases
eggNOG	COG0388.
HOGENOM	HOG000174480.
KO	K14663.
OMA	FPEFALT.
OrthoDB	EOG4NW2VF.
Gene expression databases
Genevestigator	P40354.
GermOnline	YJR062C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.60.110.10. 2 hits.
InterPro	IPR003010. C-N_Hydrolase. [Graphical view]
Pfam	PF00795. CN_hydrolase. 1 hit. [Graphical view]
SUPFAM	SSF56317. Ntlse/CNhydtse. 1 hit.
PROSITE	PS50263. CN_HYDROLASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974212.

Entry information

Entry name

NTA1_YEAST

Accession

Primary (citable) accession number: P40354
Secondary accession number(s): D6VWN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program