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P40354

- NTA1_YEAST

UniProt

P40354 - NTA1_YEAST

Protein

Protein N-terminal amidase

Gene

NTA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Deamidates N-terminal Asn and Gln. Component of a targeting complex in the N-end rule pathway.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei63 – 631Proton acceptorPROSITE-ProRule annotation
    Active sitei136 – 1361Proton donorPROSITE-ProRule annotation
    Active sitei187 – 1871NucleophilePROSITE-ProRule annotation

    GO - Molecular functioni

    1. protein-N-terminal asparagine amidohydrolase activity Source: SGD
    2. protein-N-terminal glutamine amidohydrolase activity Source: SGD

    GO - Biological processi

    1. cellular protein modification process Source: SGD
    2. nitrogen compound metabolic process Source: InterPro
    3. protein catabolic process Source: SGD

    Keywords - Molecular functioni

    Hydrolase

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31695-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein N-terminal amidase (EC:3.5.1.-)
    Short name:
    NT-amidase
    Gene namesi
    Name:NTA1
    Ordered Locus Names:YJR062C
    ORF Names:J1742
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJR062c.
    SGDiS000003823. NTA1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 457457Protein N-terminal amidasePRO_0000204068Add
    BLAST

    Proteomic databases

    MaxQBiP40354.
    PaxDbiP40354.

    Expressioni

    Gene expression databases

    GenevestigatoriP40354.

    Interactioni

    Protein-protein interaction databases

    BioGridi33819. 22 interactions.
    DIPiDIP-6478N.
    IntActiP40354. 8 interactions.
    MINTiMINT-673827.
    STRINGi4932.YJR062C.

    Structurei

    3D structure databases

    ProteinModelPortaliP40354.
    SMRiP40354. Positions 19-164.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini19 – 319301CN hydrolasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 CN hydrolase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0388.
    HOGENOMiHOG000174480.
    KOiK14663.
    OMAiGICMDLS.
    OrthoDBiEOG7ZSJ4C.

    Family and domain databases

    Gene3Di3.60.110.10. 2 hits.
    InterProiIPR003010. C-N_Hydrolase.
    [Graphical view]
    PfamiPF00795. CN_hydrolase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56317. SSF56317. 1 hit.
    PROSITEiPS50263. CN_HYDROLASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40354-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLIDAIHGAK MSTKLLVSLK VLVIQLNPQI GQVDQTIKRT WSILDKVTKS    50
    ATYVKPDIIL FPEFALTGYS FHARKDILPY VTKKDEGPSF ELAKSISEKF 100
    QCYTIIGYPE DDDEQKLYNS ALVVNPQGEQ IFNYRKTFLY DTEMNWDCEE 150
    NPEGFQTFPM DFSKCAKLSN EDSYNRDVTL KASIGICMDL SPYKFMAPFN 200
    HFEFSSFCVD NNVELILCPM AWLNSTSITD KQTLHNNSLL EAAKNKIAFA 250
    LKEQGLPLAG SQGIYQLKIG DSQRTPRVPS DDSTSEYKDM DEPDMSNVNY 300
    WILRFFPFLY FKSRINWFKN SSLIESILGK TRMPLDHEYY KDGKHKEDTI 350
    DLLDSEEVIK DTVLEKTFLG TSLGQPWKFQ GKNAILVLAN RCGTEDGTTI 400
    FAGSSGIYKF NGKKPKGSQD DDESSLDSLN ESVELLGNLG KGLEGAILRE 450
    VQFEVFR 457
    Length:457
    Mass (Da):51,897
    Last modified:February 1, 1995 - v1
    Checksum:iE6259FBBFB25A889
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L35564 Genomic DNA. Translation: AAB59320.1.
    Z49562 Genomic DNA. Translation: CAA89590.1.
    L47993 Genomic DNA. Translation: AAB39288.1.
    BK006943 Genomic DNA. Translation: DAA08849.1.
    PIRiS47938.
    RefSeqiNP_012596.3. NM_001181720.3.

    Genome annotation databases

    EnsemblFungiiYJR062C; YJR062C; YJR062C.
    GeneIDi853525.
    KEGGisce:YJR062C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L35564 Genomic DNA. Translation: AAB59320.1 .
    Z49562 Genomic DNA. Translation: CAA89590.1 .
    L47993 Genomic DNA. Translation: AAB39288.1 .
    BK006943 Genomic DNA. Translation: DAA08849.1 .
    PIRi S47938.
    RefSeqi NP_012596.3. NM_001181720.3.

    3D structure databases

    ProteinModelPortali P40354.
    SMRi P40354. Positions 19-164.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33819. 22 interactions.
    DIPi DIP-6478N.
    IntActi P40354. 8 interactions.
    MINTi MINT-673827.
    STRINGi 4932.YJR062C.

    Proteomic databases

    MaxQBi P40354.
    PaxDbi P40354.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJR062C ; YJR062C ; YJR062C .
    GeneIDi 853525.
    KEGGi sce:YJR062C.

    Organism-specific databases

    CYGDi YJR062c.
    SGDi S000003823. NTA1.

    Phylogenomic databases

    eggNOGi COG0388.
    HOGENOMi HOG000174480.
    KOi K14663.
    OMAi GICMDLS.
    OrthoDBi EOG7ZSJ4C.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31695-MONOMER.

    Miscellaneous databases

    NextBioi 974212.

    Gene expression databases

    Genevestigatori P40354.

    Family and domain databases

    Gene3Di 3.60.110.10. 2 hits.
    InterProi IPR003010. C-N_Hydrolase.
    [Graphical view ]
    Pfami PF00795. CN_hydrolase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56317. SSF56317. 1 hit.
    PROSITEi PS50263. CN_HYDROLASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Yeast N-terminal amidase. A new enzyme and component of the N-end rule pathway."
      Baker R.T., Varshavsky A.
      J. Biol. Chem. 270:12065-12074(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI."
      Huang M.-E., Manus V., Chuat J.-C., Galibert F.
      Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNTA1_YEAST
    AccessioniPrimary (citable) accession number: P40354
    Secondary accession number(s): D6VWN3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1640 molecules/cell in log phase SD medium.1 Publication

    Caution

    It is uncertain whether Met-1 or Met-11 is the initiator.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3