SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P40354

- NTA1_YEAST

UniProt

P40354 - NTA1_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Protein N-terminal amidase
Gene
NTA1, YJR062C, J1742
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Deamidates N-terminal Asn and Gln. Component of a targeting complex in the N-end rule pathway.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei63 – 631Proton acceptor By similarity
Active sitei136 – 1361Proton donor By similarity
Active sitei187 – 1871Nucleophile By similarity

GO - Molecular functioni

  1. protein-N-terminal asparagine amidohydrolase activity Source: SGD
  2. protein-N-terminal glutamine amidohydrolase activity Source: SGD

GO - Biological processi

  1. cellular protein modification process Source: SGD
  2. nitrogen compound metabolic process Source: InterPro
  3. protein catabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciYEAST:G3O-31695-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein N-terminal amidase (EC:3.5.1.-)
Short name:
NT-amidase
Gene namesi
Name:NTA1
Ordered Locus Names:YJR062C
ORF Names:J1742
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJR062c.
SGDiS000003823. NTA1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 457457Protein N-terminal amidase
PRO_0000204068Add
BLAST

Proteomic databases

MaxQBiP40354.
PaxDbiP40354.

Expressioni

Gene expression databases

GenevestigatoriP40354.

Interactioni

Protein-protein interaction databases

BioGridi33819. 22 interactions.
DIPiDIP-6478N.
IntActiP40354. 8 interactions.
MINTiMINT-673827.
STRINGi4932.YJR062C.

Structurei

3D structure databases

ProteinModelPortaliP40354.
SMRiP40354. Positions 19-164.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 319301CN hydrolase
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0388.
HOGENOMiHOG000174480.
KOiK14663.
OMAiGICMDLS.
OrthoDBiEOG7ZSJ4C.

Family and domain databases

Gene3Di3.60.110.10. 2 hits.
InterProiIPR003010. C-N_Hydrolase.
[Graphical view]
PfamiPF00795. CN_hydrolase. 1 hit.
[Graphical view]
SUPFAMiSSF56317. SSF56317. 1 hit.
PROSITEiPS50263. CN_HYDROLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40354-1 [UniParc]FASTAAdd to Basket

« Hide

MLIDAIHGAK MSTKLLVSLK VLVIQLNPQI GQVDQTIKRT WSILDKVTKS    50
ATYVKPDIIL FPEFALTGYS FHARKDILPY VTKKDEGPSF ELAKSISEKF 100
QCYTIIGYPE DDDEQKLYNS ALVVNPQGEQ IFNYRKTFLY DTEMNWDCEE 150
NPEGFQTFPM DFSKCAKLSN EDSYNRDVTL KASIGICMDL SPYKFMAPFN 200
HFEFSSFCVD NNVELILCPM AWLNSTSITD KQTLHNNSLL EAAKNKIAFA 250
LKEQGLPLAG SQGIYQLKIG DSQRTPRVPS DDSTSEYKDM DEPDMSNVNY 300
WILRFFPFLY FKSRINWFKN SSLIESILGK TRMPLDHEYY KDGKHKEDTI 350
DLLDSEEVIK DTVLEKTFLG TSLGQPWKFQ GKNAILVLAN RCGTEDGTTI 400
FAGSSGIYKF NGKKPKGSQD DDESSLDSLN ESVELLGNLG KGLEGAILRE 450
VQFEVFR 457
Length:457
Mass (Da):51,897
Last modified:February 1, 1995 - v1
Checksum:iE6259FBBFB25A889
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L35564 Genomic DNA. Translation: AAB59320.1.
Z49562 Genomic DNA. Translation: CAA89590.1.
L47993 Genomic DNA. Translation: AAB39288.1.
BK006943 Genomic DNA. Translation: DAA08849.1.
PIRiS47938.
RefSeqiNP_012596.3. NM_001181720.3.

Genome annotation databases

EnsemblFungiiYJR062C; YJR062C; YJR062C.
GeneIDi853525.
KEGGisce:YJR062C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L35564 Genomic DNA. Translation: AAB59320.1 .
Z49562 Genomic DNA. Translation: CAA89590.1 .
L47993 Genomic DNA. Translation: AAB39288.1 .
BK006943 Genomic DNA. Translation: DAA08849.1 .
PIRi S47938.
RefSeqi NP_012596.3. NM_001181720.3.

3D structure databases

ProteinModelPortali P40354.
SMRi P40354. Positions 19-164.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33819. 22 interactions.
DIPi DIP-6478N.
IntActi P40354. 8 interactions.
MINTi MINT-673827.
STRINGi 4932.YJR062C.

Proteomic databases

MaxQBi P40354.
PaxDbi P40354.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJR062C ; YJR062C ; YJR062C .
GeneIDi 853525.
KEGGi sce:YJR062C.

Organism-specific databases

CYGDi YJR062c.
SGDi S000003823. NTA1.

Phylogenomic databases

eggNOGi COG0388.
HOGENOMi HOG000174480.
KOi K14663.
OMAi GICMDLS.
OrthoDBi EOG7ZSJ4C.

Enzyme and pathway databases

BioCyci YEAST:G3O-31695-MONOMER.

Miscellaneous databases

NextBioi 974212.

Gene expression databases

Genevestigatori P40354.

Family and domain databases

Gene3Di 3.60.110.10. 2 hits.
InterProi IPR003010. C-N_Hydrolase.
[Graphical view ]
Pfami PF00795. CN_hydrolase. 1 hit.
[Graphical view ]
SUPFAMi SSF56317. SSF56317. 1 hit.
PROSITEi PS50263. CN_HYDROLASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Yeast N-terminal amidase. A new enzyme and component of the N-end rule pathway."
    Baker R.T., Varshavsky A.
    J. Biol. Chem. 270:12065-12074(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI."
    Huang M.-E., Manus V., Chuat J.-C., Galibert F.
    Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiNTA1_YEAST
AccessioniPrimary (citable) accession number: P40354
Secondary accession number(s): D6VWN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 11, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1640 molecules/cell in log phase SD medium.

Caution

It is uncertain whether Met-1 or Met-11 is the initiator.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi