P40353 (ATF1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alcohol O-acetyltransferase 1
Short name=AATase 1
EC=2.3.1.84

Gene names

Name:	ATF1
Ordered Locus Names:	YOR377W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the esterification of isoamyl alcohol and various other alcohols by acetyl-CoA.
Catalytic activity	Acetyl-CoA + an alcohol = CoA + an acetyl ester.
Enzyme regulation	Found to be inhibited by cadmium, copper, zinc and mercurium divalent cations and sulfhydryl reagents. Inhibited by the addition of unsaturated fatty acids to the culture.
Subcellular location	Membrane; Peripheral membrane protein.
Induction	Repressed under aerobic conditions.
Miscellaneous	Present with 1990 molecules/cell in log phase SD medium.
Sequence similarities	To yeast ATF2.
Biophysicochemical properties	pH dependence: Optimum pH is 8. Temperature dependence: Optimum temperature is 25 degrees Celsius.

Ontologies

Keywords
Cellular component	Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	acetate ester metabolic process Inferred from mutant phenotype PubMed 12957907. Source: SGD alcohol metabolic process Inferred from electronic annotation. Source: InterPro fermentation Inferred from mutant phenotype PubMed 12957907. Source: SGD
Cellular_component	lipid particle Inferred from direct assay PubMed 15042596. Source: SGD membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	alcohol O-acetyltransferase activity Inferred from direct assay Ref.1. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 525	525	Alcohol O-acetyltransferase 1		PRO_0000064720
Experimental info
	Sequence conflict	84	1	T → I in BAA05552. Ref.1
	Sequence conflict	391	1	N → K in BAA05552. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P40353 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 79D0733CDDC5C629	FASTA	525	61,036
	10 20 30 40 50 60 MNEIDEKNQA PVQQECLKEM IQNGHARRMG SVEDLYVALN RQNLYRNFCT YGELSDYCTR 70 80 90 100 110 120 DQLTLALREI CLKNPTLLHI VLPTRWPNHE NYYRSSEYYS RPHPVHDYIS VLQELKLSGV 130 140 150 160 170 180 VLNEQPEYSA VMKQILEEFK NSKGSYTAKI FKLTTTLTIP YFGPTGPSWR LICLPEEHTE 190 200 210 220 230 240 KWKKFIFVSN HCMSDGRSSI HFFHDLRDEL NNIKTPPKKL DYIFKYEEDY QLLRKLPEPI 250 260 270 280 290 300 EKVIDFRPPY LFIPKSLLSG FIYNHLRFSS KGVCMRMDDV EKTDDVVTEI INISPTEFQA 310 320 330 340 350 360 IKANIKSNIQ GKCTITPFLH VCWFVSLHKW GKFFKPLNFE WLTDIFIPAD CRSQLPDDDE 370 380 390 400 410 420 MRQMYRYGAN VGFIDFTPWI SEFDMNDNKE NFWPLIEHYH EVISEALRNK KHLHGLGFNI 430 440 450 460 470 480 QGFVQKYVNI DKVMCDRAIG KRRGGTLLSN VGLFNQLEEP DAKYSICDLA FGQFQGSWHQ 490 500 510 520 AFSLGVCSTN VKGMNIVVAS TKNVVGSQES LEELCSIYKA LLLGP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, sequence analysis, and expression of the yeast alcohol acetyltransferase gene." Fujii T., Nagasawa N., Iwamatsu A., Bogaki T., Tamai Y., Hamachi M. Appl. Environ. Microbiol. 60:2786-2792(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: Kyokai No.7.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV." Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K. Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The purification, properties and internal peptide sequences of alcohol acetyltransferase isolated from Saccharomyces cerevisiae Kyokai No. 7." Minetoki T., Bogaki T., Iwamatsu A., Fujii T., Hamachi M. Biosci. Biotechnol. Biochem. 57:2094-2098(1993) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D26554 Genomic DNA. Translation: BAA05552.1. Z75285 Genomic DNA. Translation: CAA99708.1. BK006948 Genomic DNA. Translation: DAA11136.1.
PIR	S67289.
RefSeq	NP_015022.3. NM_001183797.3.
3D structure databases
ProteinModelPortal	P40353.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5494N.
IntAct	P40353. 2 interactions.
MINT	MINT-523184.
Proteomic databases
PaxDb	P40353.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YOR377W; YOR377W; YOR377W.
GeneID	854559.
KEGG	sce:YOR377W. sce:YOR380W.
Organism-specific databases
CYGD	YOR377w.
SGD	S000005904. ATF1.
Phylogenomic databases
eggNOG	NOG86810.
GeneTree	ENSGT00390000000257.
HOGENOM	HOG000034125.
KO	K00664.
OrthoDB	EOG4F21C0.
Gene expression databases
ArrayExpress	P40353.
Genevestigator	P40353.
GermOnline	YOR377W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR010828. Alc_AcTrfase. [Graphical view]
Pfam	PF07247. AATase. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	976991.

Entry information

Entry name

ATF1_YEAST

Accession

Primary (citable) accession number: P40353
Secondary accession number(s): D6W370, Q08901

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program