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Protein

Alcohol O-acetyltransferase 1

Gene

ATF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the esterification of isoamyl alcohol and various other alcohols by acetyl-CoA.

Catalytic activityi

Acetyl-CoA + an alcohol = CoA + an acetyl ester.

Enzyme regulationi

Found to be inhibited by cadmium, copper, zinc and mercurium divalent cations and sulfhydryl reagents. Inhibited by the addition of unsaturated fatty acids to the culture.

pH dependencei

Optimum pH is 8.

Temperature dependencei

Optimum temperature is 25 degrees Celsius.

GO - Molecular functioni

  • alcohol O-acetyltransferase activity Source: SGD

GO - Biological processi

  • acetate ester metabolic process involved in fermentation Source: SGD
  • alcohol metabolic process Source: InterPro
  • interspecies interaction between organisms Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:YOR377W-MONOMER.
SABIO-RKP40353.

Names & Taxonomyi

Protein namesi
Recommended name:
Alcohol O-acetyltransferase 1 (EC:2.3.1.84)
Short name:
AATase 1
Gene namesi
Name:ATF1
Ordered Locus Names:YOR377W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR377W.
SGDiS000005904. ATF1.

Subcellular locationi

GO - Cellular componenti

  • lipid particle Source: SGD
  • membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 525525Alcohol O-acetyltransferase 1PRO_0000064720Add
BLAST

Proteomic databases

MaxQBiP40353.

Expressioni

Inductioni

Repressed under aerobic conditions.

Interactioni

Protein-protein interaction databases

BioGridi34759. 22 interactions.
DIPiDIP-5494N.
IntActiP40353. 2 interactions.
MINTiMINT-523184.

Structurei

3D structure databases

ProteinModelPortaliP40353.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

To yeast ATF2.Curated

Phylogenomic databases

GeneTreeiENSGT00390000000257.
HOGENOMiHOG000034125.
InParanoidiP40353.
KOiK00664.
OrthoDBiEOG7DNP43.

Family and domain databases

InterProiIPR010828. Alc_AcTrfase.
[Graphical view]
PfamiPF07247. AATase. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40353-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEIDEKNQA PVQQECLKEM IQNGHARRMG SVEDLYVALN RQNLYRNFCT
60 70 80 90 100
YGELSDYCTR DQLTLALREI CLKNPTLLHI VLPTRWPNHE NYYRSSEYYS
110 120 130 140 150
RPHPVHDYIS VLQELKLSGV VLNEQPEYSA VMKQILEEFK NSKGSYTAKI
160 170 180 190 200
FKLTTTLTIP YFGPTGPSWR LICLPEEHTE KWKKFIFVSN HCMSDGRSSI
210 220 230 240 250
HFFHDLRDEL NNIKTPPKKL DYIFKYEEDY QLLRKLPEPI EKVIDFRPPY
260 270 280 290 300
LFIPKSLLSG FIYNHLRFSS KGVCMRMDDV EKTDDVVTEI INISPTEFQA
310 320 330 340 350
IKANIKSNIQ GKCTITPFLH VCWFVSLHKW GKFFKPLNFE WLTDIFIPAD
360 370 380 390 400
CRSQLPDDDE MRQMYRYGAN VGFIDFTPWI SEFDMNDNKE NFWPLIEHYH
410 420 430 440 450
EVISEALRNK KHLHGLGFNI QGFVQKYVNI DKVMCDRAIG KRRGGTLLSN
460 470 480 490 500
VGLFNQLEEP DAKYSICDLA FGQFQGSWHQ AFSLGVCSTN VKGMNIVVAS
510 520
TKNVVGSQES LEELCSIYKA LLLGP
Length:525
Mass (Da):61,036
Last modified:November 1, 1997 - v2
Checksum:i79D0733CDDC5C629
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti84 – 841T → I in BAA05552 (PubMed:8085822).Curated
Sequence conflicti391 – 3911N → K in BAA05552 (PubMed:8085822).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26554 Genomic DNA. Translation: BAA05552.1.
Z75285 Genomic DNA. Translation: CAA99708.1.
BK006948 Genomic DNA. Translation: DAA11136.1.
PIRiS67289.
RefSeqiNP_015022.3. NM_001183797.3.

Genome annotation databases

EnsemblFungiiYOR377W; YOR377W; YOR377W.
GeneIDi854559.
KEGGisce:YOR377W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26554 Genomic DNA. Translation: BAA05552.1.
Z75285 Genomic DNA. Translation: CAA99708.1.
BK006948 Genomic DNA. Translation: DAA11136.1.
PIRiS67289.
RefSeqiNP_015022.3. NM_001183797.3.

3D structure databases

ProteinModelPortaliP40353.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34759. 22 interactions.
DIPiDIP-5494N.
IntActiP40353. 2 interactions.
MINTiMINT-523184.

Proteomic databases

MaxQBiP40353.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR377W; YOR377W; YOR377W.
GeneIDi854559.
KEGGisce:YOR377W.

Organism-specific databases

EuPathDBiFungiDB:YOR377W.
SGDiS000005904. ATF1.

Phylogenomic databases

GeneTreeiENSGT00390000000257.
HOGENOMiHOG000034125.
InParanoidiP40353.
KOiK00664.
OrthoDBiEOG7DNP43.

Enzyme and pathway databases

BioCyciYEAST:YOR377W-MONOMER.
SABIO-RKP40353.

Miscellaneous databases

NextBioi976991.
PROiP40353.

Family and domain databases

InterProiIPR010828. Alc_AcTrfase.
[Graphical view]
PfamiPF07247. AATase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, sequence analysis, and expression of the yeast alcohol acetyltransferase gene."
    Fujii T., Nagasawa N., Iwamatsu A., Bogaki T., Tamai Y., Hamachi M.
    Appl. Environ. Microbiol. 60:2786-2792(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: Kyokai No.7.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The purification, properties and internal peptide sequences of alcohol acetyltransferase isolated from Saccharomyces cerevisiae Kyokai No. 7."
    Minetoki T., Bogaki T., Iwamatsu A., Fujii T., Hamachi M.
    Biosci. Biotechnol. Biochem. 57:2094-2098(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiATF1_YEAST
AccessioniPrimary (citable) accession number: P40353
Secondary accession number(s): D6W370, Q08901
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1997
Last modified: May 11, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1990 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.