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P40352

- RAD26_YEAST

UniProt

P40352 - RAD26_YEAST

Protein

DNA repair and recombination protein RAD26

Gene

RAD26

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    May be involved in the preferential repair of active genes.

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi322 – 3298ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA binding Source: UniProtKB-KW
    3. DNA-dependent ATPase activity Source: SGD
    4. helicase activity Source: UniProtKB-KW

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. histone H2A-H2B dimer displacement Source: SGD
    3. nucleotide-excision repair Source: SGD
    4. transcription-coupled nucleotide-excision repair Source: SGD

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31672-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA repair and recombination protein RAD26 (EC:3.6.4.12)
    Alternative name(s):
    ATP-dependent helicase RAD26
    Gene namesi
    Name:RAD26
    Synonyms:GTA1085
    Ordered Locus Names:YJR035W
    ORF Names:J1606
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJR035w.
    SGDiS000003796. RAD26.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10851085DNA repair and recombination protein RAD26PRO_0000074336Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei30 – 301Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP40352.
    PaxDbiP40352.
    PeptideAtlasiP40352.

    Expressioni

    Gene expression databases

    GenevestigatoriP40352.

    Interactioni

    Protein-protein interaction databases

    BioGridi33788. 110 interactions.
    DIPiDIP-3008N.
    IntActiP40352. 2 interactions.
    MINTiMINT-439055.
    STRINGi4932.YJR035W.

    Structurei

    3D structure databases

    ProteinModelPortaliP40352.
    SMRiP40352. Positions 300-825.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini309 – 518210Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini655 – 818164Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi469 – 4724DEGH box

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi173 – 22856Asp/Glu-rich (acidic)Add
    BLAST

    Sequence similaritiesi

    Belongs to the SNF2/RAD54 helicase family.Curated
    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0553.
    GeneTreeiENSGT00590000083118.
    HOGENOMiHOG000170952.
    KOiK10841.
    OMAiQLLLLWH.
    OrthoDBiEOG7SXWC3.

    Family and domain databases

    Gene3Di3.40.50.300. 3 hits.
    InterProiIPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000330. SNF2_N.
    [Graphical view]
    PfamiPF00271. Helicase_C. 1 hit.
    PF00176. SNF2_N. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 3 hits.
    PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40352-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEDKEQQDNA KLENNESLKD LGVNVLSQSS LEEKIANDVT NFSNLQSLQQ     50
    EETRLERSKT ALQRYVNKKN HLTRKLNNTT RISVKQNLRD QIKNLQSDDI 100
    ERVLKDIDDI QSRIKELKEQ VDQGAENKGS KEGLQRPGET EKEFLIRTGK 150
    ITAFGHKAGF SLDTANREYA KNDEQKDEDF EMATEQMVEN LTDEDDNLSD 200
    QDYQMSGKES EDDEEEENDD KILKELEDLR FRGQPGEAKD DGDELYYQER 250
    LKKWVKQRSC GSQRSSDLPE WRRPHPNIPD AKLNSQFKIP GEIYSLLFNY 300
    QKTCVQWLYE LYQQNCGGII GDEMGLGKTI QVIAFIAALH HSGLLTGPVL 350
    IVCPATVMKQ WCNEFQHWWP PLRTVILHSM GSGMASDQKF KMDENDLENL 400
    IMNSKPSDFS YEDWKNSTRT KKALESSYHL DKLIDKVVTD GHILITTYVG 450
    LRIHSDKLLK VKWQYAVLDE GHKIRNPDSE ISLTCKKLKT HNRIILSGTP 500
    IQNNLTELWS LFDFIFPGKL GTLPVFQQQF VIPINIGGYA NATNIQVQTG 550
    YKCAVALRDL ISPYLLRRVK ADVAKDLPQK KEMVLFCKLT KYQRSKYLEF 600
    LHSSDLNQIQ NGKRNVLFGI DILRKICNHP DLLDRDTKRH NPDYGDPKRS 650
    GKMQVVKQLL LLWHKQGYKA LLFTQSRQML DILEEFISTK DPDLSHLNYL 700
    RMDGTTNIKG RQSLVDRFNN ESFDVFLLTT RVGGLGVNLT GANRIIIFDP 750
    DWNPSTDMQA RERAWRIGQK REVSIYRLMV GGSIEEKIYH RQIFKQFLTN 800
    RILTDPKQKR FFKIHELHDL FSLGGENGYS TEELNEEVQK HTENLKNSKS 850
    EESDDFEQLV NLSGVSKLES FYNGKEKKEN SKTEDDRLIE GLLGGESNLE 900
    TVMSHDSVVN SHAGSSSSNI ITKEASRVAI EAVNALRKSR KKITKQYEIG 950
    TPTWTGRFGK AGKIRKRDPL KNKLTGSAAI LGNITKSQKE ASKEARQENY 1000
    DDGITFARSK EINSNTKTLE NIRAYLQKQN NFFSSSVSIL NSIGVSLSDK 1050
    EDVIKVRALL KTIAQFDKER KGWVLDEEFR NNNAS 1085
    Length:1,085
    Mass (Da):124,528
    Last modified:February 1, 1995 - v1
    Checksum:i2A8C34A7300148E3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti366 – 3661Q → H in CAA57290. (PubMed:7957102)Curated
    Sequence conflicti963 – 9631K → E in CAA57290. (PubMed:7957102)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L26910 Genomic DNA. Translation: AAA34655.1.
    X81635 Genomic DNA. Translation: CAA57290.1.
    Z49535 Genomic DNA. Translation: CAA89562.1.
    BK006943 Genomic DNA. Translation: DAA08824.1.
    PIRiJC2227.
    RefSeqiNP_012569.1. NM_001181693.1.

    Genome annotation databases

    EnsemblFungiiYJR035W; YJR035W; YJR035W.
    GeneIDi853492.
    KEGGisce:YJR035W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L26910 Genomic DNA. Translation: AAA34655.1 .
    X81635 Genomic DNA. Translation: CAA57290.1 .
    Z49535 Genomic DNA. Translation: CAA89562.1 .
    BK006943 Genomic DNA. Translation: DAA08824.1 .
    PIRi JC2227.
    RefSeqi NP_012569.1. NM_001181693.1.

    3D structure databases

    ProteinModelPortali P40352.
    SMRi P40352. Positions 300-825.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33788. 110 interactions.
    DIPi DIP-3008N.
    IntActi P40352. 2 interactions.
    MINTi MINT-439055.
    STRINGi 4932.YJR035W.

    Proteomic databases

    MaxQBi P40352.
    PaxDbi P40352.
    PeptideAtlasi P40352.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJR035W ; YJR035W ; YJR035W .
    GeneIDi 853492.
    KEGGi sce:YJR035W.

    Organism-specific databases

    CYGDi YJR035w.
    SGDi S000003796. RAD26.

    Phylogenomic databases

    eggNOGi COG0553.
    GeneTreei ENSGT00590000083118.
    HOGENOMi HOG000170952.
    KOi K10841.
    OMAi QLLLLWH.
    OrthoDBi EOG7SXWC3.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31672-MONOMER.

    Miscellaneous databases

    NextBioi 974125.
    PROi P40352.

    Gene expression databases

    Genevestigatori P40352.

    Family and domain databases

    Gene3Di 3.40.50.300. 3 hits.
    InterProi IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000330. SNF2_N.
    [Graphical view ]
    Pfami PF00271. Helicase_C. 1 hit.
    PF00176. SNF2_N. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 3 hits.
    PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A possible yeast homolog of human active-gene-repairing helicase ERCC6+."
      Huang M.-E., Chuat J.-C., Galibert F.
      Biochem. Biophys. Res. Commun. 201:310-317(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "RAD26, the functional S. cerevisiae homolog of the Cockayne syndrome B gene ERCC6."
      van Gool A.J., Verhage R., Swagemakers S.M.A., van de Putte P., Brouwer J., Troelstra C., Bootsma D., Hoeijmakers J.H.J.
      EMBO J. 13:5361-5369(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-30, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRAD26_YEAST
    AccessioniPrimary (citable) accession number: P40352
    Secondary accession number(s): D6VWK8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3