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Protein

DNA repair and recombination protein RAD26

Gene

RAD26

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in the preferential repair of active genes.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi322 – 3298ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA-dependent ATPase activity Source: SGD
  4. helicase activity Source: UniProtKB-KW

GO - Biological processi

  1. histone H2A-H2B dimer displacement Source: SGD
  2. nucleotide-excision repair Source: SGD
  3. transcription-coupled nucleotide-excision repair Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31672-MONOMER.
ReactomeiREACT_273984. Dual incision reaction in TC-NER.
REACT_331999. Formation of transcription-coupled NER (TC-NER) repair complex.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair and recombination protein RAD26 (EC:3.6.4.12)
Alternative name(s):
ATP-dependent helicase RAD26
Gene namesi
Name:RAD26
Synonyms:GTA1085
Ordered Locus Names:YJR035W
ORF Names:J1606
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome X

Organism-specific databases

CYGDiYJR035w.
SGDiS000003796. RAD26.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10851085DNA repair and recombination protein RAD26PRO_0000074336Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei30 – 301Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40352.
PaxDbiP40352.
PeptideAtlasiP40352.

Expressioni

Gene expression databases

GenevestigatoriP40352.

Interactioni

Protein-protein interaction databases

BioGridi33788. 110 interactions.
DIPiDIP-3008N.
IntActiP40352. 2 interactions.
MINTiMINT-439055.
STRINGi4932.YJR035W.

Structurei

3D structure databases

ProteinModelPortaliP40352.
SMRiP40352. Positions 300-823.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini309 – 518210Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini655 – 818164Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi469 – 4724DEGH box

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi173 – 22856Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0553.
GeneTreeiENSGT00590000083118.
HOGENOMiHOG000170952.
InParanoidiP40352.
KOiK10841.
OMAiQLLLLWH.
OrthoDBiEOG7SXWC3.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40352-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDKEQQDNA KLENNESLKD LGVNVLSQSS LEEKIANDVT NFSNLQSLQQ
60 70 80 90 100
EETRLERSKT ALQRYVNKKN HLTRKLNNTT RISVKQNLRD QIKNLQSDDI
110 120 130 140 150
ERVLKDIDDI QSRIKELKEQ VDQGAENKGS KEGLQRPGET EKEFLIRTGK
160 170 180 190 200
ITAFGHKAGF SLDTANREYA KNDEQKDEDF EMATEQMVEN LTDEDDNLSD
210 220 230 240 250
QDYQMSGKES EDDEEEENDD KILKELEDLR FRGQPGEAKD DGDELYYQER
260 270 280 290 300
LKKWVKQRSC GSQRSSDLPE WRRPHPNIPD AKLNSQFKIP GEIYSLLFNY
310 320 330 340 350
QKTCVQWLYE LYQQNCGGII GDEMGLGKTI QVIAFIAALH HSGLLTGPVL
360 370 380 390 400
IVCPATVMKQ WCNEFQHWWP PLRTVILHSM GSGMASDQKF KMDENDLENL
410 420 430 440 450
IMNSKPSDFS YEDWKNSTRT KKALESSYHL DKLIDKVVTD GHILITTYVG
460 470 480 490 500
LRIHSDKLLK VKWQYAVLDE GHKIRNPDSE ISLTCKKLKT HNRIILSGTP
510 520 530 540 550
IQNNLTELWS LFDFIFPGKL GTLPVFQQQF VIPINIGGYA NATNIQVQTG
560 570 580 590 600
YKCAVALRDL ISPYLLRRVK ADVAKDLPQK KEMVLFCKLT KYQRSKYLEF
610 620 630 640 650
LHSSDLNQIQ NGKRNVLFGI DILRKICNHP DLLDRDTKRH NPDYGDPKRS
660 670 680 690 700
GKMQVVKQLL LLWHKQGYKA LLFTQSRQML DILEEFISTK DPDLSHLNYL
710 720 730 740 750
RMDGTTNIKG RQSLVDRFNN ESFDVFLLTT RVGGLGVNLT GANRIIIFDP
760 770 780 790 800
DWNPSTDMQA RERAWRIGQK REVSIYRLMV GGSIEEKIYH RQIFKQFLTN
810 820 830 840 850
RILTDPKQKR FFKIHELHDL FSLGGENGYS TEELNEEVQK HTENLKNSKS
860 870 880 890 900
EESDDFEQLV NLSGVSKLES FYNGKEKKEN SKTEDDRLIE GLLGGESNLE
910 920 930 940 950
TVMSHDSVVN SHAGSSSSNI ITKEASRVAI EAVNALRKSR KKITKQYEIG
960 970 980 990 1000
TPTWTGRFGK AGKIRKRDPL KNKLTGSAAI LGNITKSQKE ASKEARQENY
1010 1020 1030 1040 1050
DDGITFARSK EINSNTKTLE NIRAYLQKQN NFFSSSVSIL NSIGVSLSDK
1060 1070 1080
EDVIKVRALL KTIAQFDKER KGWVLDEEFR NNNAS
Length:1,085
Mass (Da):124,528
Last modified:February 1, 1995 - v1
Checksum:i2A8C34A7300148E3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti366 – 3661Q → H in CAA57290 (PubMed:7957102).Curated
Sequence conflicti963 – 9631K → E in CAA57290 (PubMed:7957102).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26910 Genomic DNA. Translation: AAA34655.1.
X81635 Genomic DNA. Translation: CAA57290.1.
Z49535 Genomic DNA. Translation: CAA89562.1.
BK006943 Genomic DNA. Translation: DAA08824.1.
PIRiJC2227.
RefSeqiNP_012569.1. NM_001181693.1.

Genome annotation databases

EnsemblFungiiYJR035W; YJR035W; YJR035W.
GeneIDi853492.
KEGGisce:YJR035W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L26910 Genomic DNA. Translation: AAA34655.1.
X81635 Genomic DNA. Translation: CAA57290.1.
Z49535 Genomic DNA. Translation: CAA89562.1.
BK006943 Genomic DNA. Translation: DAA08824.1.
PIRiJC2227.
RefSeqiNP_012569.1. NM_001181693.1.

3D structure databases

ProteinModelPortaliP40352.
SMRiP40352. Positions 300-823.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33788. 110 interactions.
DIPiDIP-3008N.
IntActiP40352. 2 interactions.
MINTiMINT-439055.
STRINGi4932.YJR035W.

Proteomic databases

MaxQBiP40352.
PaxDbiP40352.
PeptideAtlasiP40352.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR035W; YJR035W; YJR035W.
GeneIDi853492.
KEGGisce:YJR035W.

Organism-specific databases

CYGDiYJR035w.
SGDiS000003796. RAD26.

Phylogenomic databases

eggNOGiCOG0553.
GeneTreeiENSGT00590000083118.
HOGENOMiHOG000170952.
InParanoidiP40352.
KOiK10841.
OMAiQLLLLWH.
OrthoDBiEOG7SXWC3.

Enzyme and pathway databases

BioCyciYEAST:G3O-31672-MONOMER.
ReactomeiREACT_273984. Dual incision reaction in TC-NER.
REACT_331999. Formation of transcription-coupled NER (TC-NER) repair complex.

Miscellaneous databases

NextBioi974125.
PROiP40352.

Gene expression databases

GenevestigatoriP40352.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A possible yeast homolog of human active-gene-repairing helicase ERCC6+."
    Huang M.-E., Chuat J.-C., Galibert F.
    Biochem. Biophys. Res. Commun. 201:310-317(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "RAD26, the functional S. cerevisiae homolog of the Cockayne syndrome B gene ERCC6."
    van Gool A.J., Verhage R., Swagemakers S.M.A., van de Putte P., Brouwer J., Troelstra C., Bootsma D., Hoeijmakers J.H.J.
    EMBO J. 13:5361-5369(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-30, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRAD26_YEAST
AccessioniPrimary (citable) accession number: P40352
Secondary accession number(s): D6VWK8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 1, 2015
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.