DNA repair and recombination protein RAD26 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Preferred order of sections

Names and origin

Protein names

Recommended name:
DNA repair and recombination protein RAD26
EC=3.6.4.12

Alternative name(s):
ATP-dependent helicase RAD26

Gene names

Name:	RAD26
Synonyms:	GTA1085
Ordered Locus Names:	YJR035W
ORF Names:	J1606

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	1085 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in the preferential repair of active genes.
Catalytic activity	ATP + H₂O = ADP + phosphate.
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the SNF2/RAD54 helicase family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transcription-coupled nucleotide-excision repair Inferred from mutant phenotype Ref.2. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 14562095. Source: SGD nucleus Inferred from direct assay PubMed 14562095. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-dependent ATPase activity Inferred from direct assay PubMed 8702468. Source: SGD helicase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1085

1085

DNA repair and recombination protein RAD26

PRO_0000074336

Regions

Domain

309 – 518

210

Helicase ATP-binding

Domain

655 – 818

164

Helicase C-terminal

Nucleotide binding

322 – 329

8

ATP Potential

Motif

469 – 472

4

DEGH box

Compositional bias

173 – 228

56

Asp/Glu-rich (acidic)

Amino acid modifications

Modified residue

27

1

Phosphoserine Ref.5

Modified residue

29

1

Phosphoserine Ref.5

Experimental info

Sequence conflict

366

1

Q → H in CAA57290. Ref.2

Sequence conflict

963

1

K → E in CAA57290. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P40352 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 2A8C34A7300148E3
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FASTA

1,085

124,528

        10         20         30         40         50         60 
MEDKEQQDNA KLENNESLKD LGVNVLSQSS LEEKIANDVT NFSNLQSLQQ EETRLERSKT 

        70         80         90        100        110        120 
ALQRYVNKKN HLTRKLNNTT RISVKQNLRD QIKNLQSDDI ERVLKDIDDI QSRIKELKEQ 

       130        140        150        160        170        180 
VDQGAENKGS KEGLQRPGET EKEFLIRTGK ITAFGHKAGF SLDTANREYA KNDEQKDEDF 

       190        200        210        220        230        240 
EMATEQMVEN LTDEDDNLSD QDYQMSGKES EDDEEEENDD KILKELEDLR FRGQPGEAKD 

       250        260        270        280        290        300 
DGDELYYQER LKKWVKQRSC GSQRSSDLPE WRRPHPNIPD AKLNSQFKIP GEIYSLLFNY 

       310        320        330        340        350        360 
QKTCVQWLYE LYQQNCGGII GDEMGLGKTI QVIAFIAALH HSGLLTGPVL IVCPATVMKQ 

       370        380        390        400        410        420 
WCNEFQHWWP PLRTVILHSM GSGMASDQKF KMDENDLENL IMNSKPSDFS YEDWKNSTRT 

       430        440        450        460        470        480 
KKALESSYHL DKLIDKVVTD GHILITTYVG LRIHSDKLLK VKWQYAVLDE GHKIRNPDSE 

       490        500        510        520        530        540 
ISLTCKKLKT HNRIILSGTP IQNNLTELWS LFDFIFPGKL GTLPVFQQQF VIPINIGGYA 

       550        560        570        580        590        600 
NATNIQVQTG YKCAVALRDL ISPYLLRRVK ADVAKDLPQK KEMVLFCKLT KYQRSKYLEF 

       610        620        630        640        650        660 
LHSSDLNQIQ NGKRNVLFGI DILRKICNHP DLLDRDTKRH NPDYGDPKRS GKMQVVKQLL 

       670        680        690        700        710        720 
LLWHKQGYKA LLFTQSRQML DILEEFISTK DPDLSHLNYL RMDGTTNIKG RQSLVDRFNN 

       730        740        750        760        770        780 
ESFDVFLLTT RVGGLGVNLT GANRIIIFDP DWNPSTDMQA RERAWRIGQK REVSIYRLMV 

       790        800        810        820        830        840 
GGSIEEKIYH RQIFKQFLTN RILTDPKQKR FFKIHELHDL FSLGGENGYS TEELNEEVQK 

       850        860        870        880        890        900 
HTENLKNSKS EESDDFEQLV NLSGVSKLES FYNGKEKKEN SKTEDDRLIE GLLGGESNLE 

       910        920        930        940        950        960 
TVMSHDSVVN SHAGSSSSNI ITKEASRVAI EAVNALRKSR KKITKQYEIG TPTWTGRFGK 

       970        980        990       1000       1010       1020 
AGKIRKRDPL KNKLTGSAAI LGNITKSQKE ASKEARQENY DDGITFARSK EINSNTKTLE 

      1030       1040       1050       1060       1070       1080 
NIRAYLQKQN NFFSSSVSIL NSIGVSLSDK EDVIKVRALL KTIAQFDKER KGWVLDEEFR 


NNNAS

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A possible yeast homolog of human active-gene-repairing helicase ERCC6+." Huang M.-E., Chuat J.-C., Galibert F. Biochem. Biophys. Res. Commun. 201:310-317(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"RAD26, the functional S. cerevisiae homolog of the Cockayne syndrome B gene ERCC6." van Gool A.J., Verhage R., Swagemakers S.M.A., van de Putte P., Brouwer J., Troelstra C., Bootsma D., Hoeijmakers J.H.J. EMBO J. 13:5361-5369(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-27 AND SER-29, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L26910 Genomic DNA. Translation: AAA34655.1. X81635 Genomic DNA. Translation: CAA57290.1. Z49535 Genomic DNA. Translation: CAA89562.1. BK006943 Genomic DNA. Translation: DAA08824.1.
PIR	JC2227.
RefSeq	NP_012569.1. NM_001181693.1.
3D structure databases
ProteinModelPortal	P40352.
SMR	P40352. Positions 300-823.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-3008N.
IntAct	P40352. 23 interactions.
MINT	MINT-439055.
STRING	4932.YJR035W.
Proteomic databases
PaxDb	P40352.
PeptideAtlas	P40352.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR035W; YJR035W; YJR035W.
GeneID	853492.
KEGG	sce:YJR035W.
Organism-specific databases
CYGD	YJR035w.
SGD	S000003796. RAD26.
Phylogenomic databases
eggNOG	COG0553.
GeneTree	ENSGT00590000083118.
HOGENOM	HOG000170952.
KO	K10841.
OMA	DENRIME.
OrthoDB	EOG44TSGZ.
Enzyme and pathway databases
BioCyc	YEAST:G3O-31672-MONOMER.
Gene expression databases
Genevestigator	P40352.
GermOnline	YJR035W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR027417. P-loop_NTPase. IPR000330. SNF2_N. [Graphical view]
Pfam	PF00271. Helicase_C. 1 hit. PF00176. SNF2_N. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 2 hits.
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974125.

Entry information

Entry name

RAD26_YEAST

Accession

Primary (citable) accession number: P40352
Secondary accession number(s): D6VWK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 29, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents