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Protein

Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase

Gene

ALG8

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Adds the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc1Man9GlcNAc(2)-PP-Dol.1 Publication

Catalytic activityi

Dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

  • oligosaccharide-lipid intermediate biosynthetic process Source: SGD
  • protein N-linked glycosylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:YOR067C-MONOMER.
YEAST:YOR067C-MONOMER.
BRENDAi2.4.1.265. 984.
ReactomeiR-SCE-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT57. Glycosyltransferase Family 57.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase (EC:2.4.1.265)
Alternative name(s):
Asparagine-linked glycosylation protein 8
Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl alpha-1,3-glucosyltransferase
Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase
Gene namesi
Name:ALG8
Ordered Locus Names:YOR067C
ORF Names:YOR29-18
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOR067C.
SGDiS000005593. ALG8.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 42LumenalSequence analysisAdd BLAST42
Transmembranei43 – 63HelicalSequence analysisAdd BLAST21
Topological domaini64 – 92CytoplasmicSequence analysisAdd BLAST29
Transmembranei93 – 113HelicalSequence analysisAdd BLAST21
Topological domaini114 – 139LumenalSequence analysisAdd BLAST26
Transmembranei140 – 160HelicalSequence analysisAdd BLAST21
Topological domaini161 – 168CytoplasmicSequence analysis8
Transmembranei169 – 189HelicalSequence analysisAdd BLAST21
Topological domaini190 – 219LumenalSequence analysisAdd BLAST30
Transmembranei220 – 240HelicalSequence analysisAdd BLAST21
Topological domaini241 – 266CytoplasmicSequence analysisAdd BLAST26
Transmembranei267 – 287HelicalSequence analysisAdd BLAST21
Topological domaini288 – 307LumenalSequence analysisAdd BLAST20
Transmembranei308 – 328HelicalSequence analysisAdd BLAST21
Topological domaini329 – 382CytoplasmicSequence analysisAdd BLAST54
Transmembranei383 – 403HelicalSequence analysisAdd BLAST21
Topological domaini404 – 419LumenalSequence analysisAdd BLAST16
Transmembranei420 – 440HelicalSequence analysisAdd BLAST21
Topological domaini441 – 447CytoplasmicSequence analysis7
Transmembranei448 – 468HelicalSequence analysisAdd BLAST21
Topological domaini469 – 470LumenalSequence analysis2
Transmembranei471 – 491HelicalSequence analysisAdd BLAST21
Topological domaini492 – 504CytoplasmicSequence analysisAdd BLAST13
Transmembranei505 – 525HelicalSequence analysisAdd BLAST21
Topological domaini526 – 543LumenalSequence analysisAdd BLAST18
Transmembranei544 – 564HelicalSequence analysisAdd BLAST21
Topological domaini565 – 577CytoplasmicSequence analysisAdd BLAST13

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001741681 – 577Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferaseAdd BLAST577

Proteomic databases

MaxQBiP40351.
PRIDEiP40351.

Interactioni

Protein-protein interaction databases

BioGridi34466. 143 interactors.
DIPiDIP-7542N.

Structurei

3D structure databases

ProteinModelPortaliP40351.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000074967.
HOGENOMiHOG000168174.
InParanoidiP40351.
KOiK03849.
OMAiWKLKYPF.
OrthoDBiEOG092C28G5.

Family and domain databases

InterProiIPR004856. Glyco_trans_ALG6/ALG8.
[Graphical view]
PANTHERiPTHR12413. PTHR12413. 2 hits.
PfamiPF03155. Alg6_Alg8. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40351-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGDRSRQNM AVTKKAKLKK NDEPKKVLKT AATEKGEGSK RYSLWNFWIS
60 70 80 90 100
TLFLKLLLIP DYFSTDFDVH RNWLAITNKL PISEWYYEHT SQWTLDYPPF
110 120 130 140 150
FAYFEWFLSQ FVPKSVRDDG CLDIVEIGKF GLPTIVFQRL TVIFSEILLF
160 170 180 190 200
VILQIYINTT KLSERSQSFV VASSIVLSPG FLIIDHIHFQ YNGFLFAILI
210 220 230 240 250
GSIVAAKNKR YILCAVLYTT AICFKHIFLY LAPCYFVFLL RAYVLNVNNF
260 270 280 290 300
KFKSYKDFLF LIRWANLLKL ATVVVGIFTI CFLPFAHQMP QVLSRLFPFS
310 320 330 340 350
RGLTHAYWAP NFWALYSFMD KILTTVMLKL PYVHTFATKF IKPPLIPQNI
360 370 380 390 400
KEINERLAAN NNGSKGLVQD VFFVILPQIP PKLTFILTIF YQVLAVLPLL
410 420 430 440 450
FDPSFKRFVG SLTLCGLASF LFGWHVHEKA IMLVIIPFTF LVGFDRRLLV
460 470 480 490 500
PFMLVASAGY VSLYPLLYKG QDFFIKTLYT YVWCIIYFAA FRKTTKISSS
510 520 530 540 550
VERRIFFLDR LALTYIFSLL PIVTVLQILD EVKWRYSFLQ KFEFLGLMIY
560 570
SVYCSLGIIS SWFALSWLYN FDELLWQ
Length:577
Mass (Da):67,385
Last modified:February 1, 1995 - v1
Checksum:iA59716F526B7B5A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75929 Genomic DNA. Translation: CAA53533.1.
Z74975 Genomic DNA. Translation: CAA99260.1.
Z70678 Genomic DNA. Translation: CAA94552.1.
BK006948 Genomic DNA. Translation: DAA10846.1.
PIRiS47961.
RefSeqiNP_014710.1. NM_001183486.1.

Genome annotation databases

EnsemblFungiiYOR067C; YOR067C; YOR067C.
GeneIDi854233.
KEGGisce:YOR067C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75929 Genomic DNA. Translation: CAA53533.1.
Z74975 Genomic DNA. Translation: CAA99260.1.
Z70678 Genomic DNA. Translation: CAA94552.1.
BK006948 Genomic DNA. Translation: DAA10846.1.
PIRiS47961.
RefSeqiNP_014710.1. NM_001183486.1.

3D structure databases

ProteinModelPortaliP40351.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34466. 143 interactors.
DIPiDIP-7542N.

Protein family/group databases

CAZyiGT57. Glycosyltransferase Family 57.

Proteomic databases

MaxQBiP40351.
PRIDEiP40351.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOR067C; YOR067C; YOR067C.
GeneIDi854233.
KEGGisce:YOR067C.

Organism-specific databases

EuPathDBiFungiDB:YOR067C.
SGDiS000005593. ALG8.

Phylogenomic databases

GeneTreeiENSGT00550000074967.
HOGENOMiHOG000168174.
InParanoidiP40351.
KOiK03849.
OMAiWKLKYPF.
OrthoDBiEOG092C28G5.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciMetaCyc:YOR067C-MONOMER.
YEAST:YOR067C-MONOMER.
BRENDAi2.4.1.265. 984.
ReactomeiR-SCE-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

PROiP40351.

Family and domain databases

InterProiIPR004856. Glyco_trans_ALG6/ALG8.
[Graphical view]
PANTHERiPTHR12413. PTHR12413. 2 hits.
PfamiPF03155. Alg6_Alg8. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALG8_YEAST
AccessioniPrimary (citable) accession number: P40351
Secondary accession number(s): D6W2D0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.