Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P40351 (ALG8_YEAST)

Last modified June 16, 2009. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase
    EC=2.4.1.-
Alternative name(s):
    Dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase
    Asparagine-linked glycosylation protein 8
Gene names
Name: ALG8
Ordered Locus Names: YOR067C
ORF Names: YOR29-18
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Hide | Top

Protein attributes

Sequence length577 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Function

Adds the second glucose residue to the lipid-linked oligosaccharide precursor for N-linked glycosylation. Transfers glucose from dolichyl phosphate glucose (Dol-P-Glc) onto the lipid-linked oligosaccharide Glc1Man9GlcNAc(2)-PP-Dol.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the ALG6/ALG8 glucosyltransferase family.

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 577577Dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase
PRO_0000174168

Regions

Topological domain1 – 4242Lumenal Potential
Transmembrane43 – 6321 Potential
Topological domain64 – 9229Cytoplasmic Potential
Transmembrane93 – 11321 Potential
Topological domain114 – 13926Lumenal Potential
Transmembrane140 – 16021 Potential
Topological domain161 – 1688Cytoplasmic Potential
Transmembrane169 – 18921 Potential
Topological domain190 – 21930Lumenal Potential
Transmembrane220 – 24021 Potential
Topological domain241 – 26626Cytoplasmic Potential
Transmembrane267 – 28721 Potential
Topological domain288 – 30720Lumenal Potential
Transmembrane308 – 32821 Potential
Topological domain329 – 38254Cytoplasmic Potential
Transmembrane383 – 40321 Potential
Topological domain404 – 41916Lumenal Potential
Transmembrane420 – 44021 Potential
Topological domain441 – 4477Cytoplasmic Potential
Transmembrane448 – 46821 Potential
Topological domain469 – 4702Lumenal Potential
Transmembrane471 – 49121 Potential
Topological domain492 – 50413Cytoplasmic Potential
Transmembrane505 – 52521 Potential
Topological domain526 – 54318Lumenal Potential
Transmembrane544 – 56421 Potential
Topological domain565 – 57713Cytoplasmic Potential

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
P40351-1 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: A59716F526B7B5A6

FASTA57767,385
        10         20         30         40         50         60 
MKGDRSRQNM AVTKKAKLKK NDEPKKVLKT AATEKGEGSK RYSLWNFWIS TLFLKLLLIP 

        70         80         90        100        110        120 
DYFSTDFDVH RNWLAITNKL PISEWYYEHT SQWTLDYPPF FAYFEWFLSQ FVPKSVRDDG 

       130        140        150        160        170        180 
CLDIVEIGKF GLPTIVFQRL TVIFSEILLF VILQIYINTT KLSERSQSFV VASSIVLSPG 

       190        200        210        220        230        240 
FLIIDHIHFQ YNGFLFAILI GSIVAAKNKR YILCAVLYTT AICFKHIFLY LAPCYFVFLL 

       250        260        270        280        290        300 
RAYVLNVNNF KFKSYKDFLF LIRWANLLKL ATVVVGIFTI CFLPFAHQMP QVLSRLFPFS 

       310        320        330        340        350        360 
RGLTHAYWAP NFWALYSFMD KILTTVMLKL PYVHTFATKF IKPPLIPQNI KEINERLAAN 

       370        380        390        400        410        420 
NNGSKGLVQD VFFVILPQIP PKLTFILTIF YQVLAVLPLL FDPSFKRFVG SLTLCGLASF 

       430        440        450        460        470        480 
LFGWHVHEKA IMLVIIPFTF LVGFDRRLLV PFMLVASAGY VSLYPLLYKG QDFFIKTLYT 

       490        500        510        520        530        540 
YVWCIIYFAA FRKTTKISSS VERRIFFLDR LALTYIFSLL PIVTVLQILD EVKWRYSFLQ 

       550        560        570 
KFEFLGLMIY SVYCSLGIIS SWFALSWLYN FDELLWQ

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"New phenotype of mutations deficient in glucosylation of the lipid-linked oligosaccharide: cloning of the ALG8 locus."
Stagjar I., Te Heesen S., Aebi M.
Proc. Natl. Acad. Sci. U.S.A. 91:5977-5981(1994) [PubMed: 8016100] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The sequence of a 54.7 kb fragment of yeast chromosome XV reveals the presence of two tRNAs and 24 new open reading frames."
Valens M., Bohn C., Daignan-Fornier B., Dang V.-D., Bolotin-Fukuhara M.
Yeast 13:379-390(1997) [PubMed: 9133743] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D. expand/collapse author list , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
Nature 387:98-102(1997) [PubMed: 9169874] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed: 16847258] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases

X75929 Genomic DNA. Translation: CAA53533.1.
Z74975 Genomic DNA. Translation: CAA99260.1.
Z70678 Genomic DNA. Translation: CAA94552.1.
PIRS47961.
RefSeqNP_014710.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:7542N.
IntActP40351. 1 interaction.

Protein family/group databases

CAZyGT57. Glycosyltransferase Family 57.

Proteomic databases

PeptideAtlasP40351.

Genome annotation databases

EnsemblYOR067C. Saccharomyces cerevisiae. [Contig view]
GeneID854233.
GenomeReviewsGene locus YOR067C in contig Y13140_GR.
KEGGsce:YOR067C.
NMPDRfig|4932.3.peg.5810.

Organism-specific databases

CYGDYOR067c.
SGDS000005593. ALG8.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMP40351.
OMAP40351. HNLNYSS.

Enzyme and pathway databases

BioCycMetaCyc:MON-7192.

Gene expression databases

GermOnlineYOR067C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR004856. Glycosyltransferase_ALG6/ALG8.
[Graphical view]
PANTHERPTHR12413. Alg6_Alg8. 1 hit.
PfamPF03155. Alg6_Alg8. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio976121.

Hide | Top

Entry information

Entry nameALG8_YEAST
AccessionPrimary (citable) accession number: P40351
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Hide | Top

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents