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Protein

Dolichyl-phosphate beta-glucosyltransferase

Gene

ALG5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

UDP-glucose + dolichyl phosphate = UDP + dolichyl beta-D-glucosyl phosphate.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • dolichyl-phosphate beta-glucosyltransferase activity Source: SGD

GO - Biological processi

  • protein N-linked glycosylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-7202.
YEAST:YPL227C-MONOMER.
ReactomeiR-SCE-480985. Synthesis of dolichyl-phosphate-glucose.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-phosphate beta-glucosyltransferase (EC:2.4.1.117)
Short name:
DolP-glucosyltransferase
Alternative name(s):
Asparagine-linked glycosylation protein 5
Gene namesi
Name:ALG5
Ordered Locus Names:YPL227C
ORF Names:P1437
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL227C.
SGDiS000006148. ALG5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence analysisAdd
BLAST
Transmembranei13 – 3321Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini34 – 334301LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum membrane Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 334334Dolichyl-phosphate beta-glucosyltransferasePRO_0000059097Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi83 – 831N-linked (GlcNAc...)Sequence analysis
Glycosylationi119 – 1191N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP40350.

Interactioni

Protein-protein interaction databases

BioGridi35958. 151 interactions.
DIPiDIP-5651N.
IntActiP40350. 25 interactions.
MINTiMINT-547084.

Structurei

3D structure databases

ProteinModelPortaliP40350.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000074941.
HOGENOMiHOG000283250.
InParanoidiP40350.
KOiK00729.
OMAiFIRLRYM.
OrthoDBiEOG77WWPJ.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

P40350-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRALRFLIEN RNTVFFTLLV ALVLSLYLLV YLFSHTPRPP YPEELKYIAI
60 70 80 90 100
DEKGHEVSRA LPNLNEHQDD EEIFLSVVIP SYNETGRILL MLTDAISFLK
110 120 130 140 150
EKYGSRWEIV IVDDGSTDNT TQYCLKICKE QFKLNYEQFR IIKFSQNRGK
160 170 180 190 200
GGAVRQGFLH IRGKYGLFAD ADGASKFSDV EKLIDAISKI ETSSTDLKTT
210 220 230 240 250
KPAVAIGSRA HMVNTEAVIK RSMIRNCLMY GFHTLVFIFG IRSIKDTQCG
260 270 280 290 300
FKLFNRAAIL KIFPYLHTEG WIFDVEILIL AIRKRIQIEE IPISWHEVDG
310 320 330
SKMALAIDSI KMAKDLVIIR MAYLLGIYRD NKKC
Length:334
Mass (Da):38,347
Last modified:February 1, 1995 - v1
Checksum:i639A88DDED879217
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77573 Genomic DNA. Translation: CAA54680.1.
X94561 Genomic DNA. Translation: CAA64260.1.
Z73583 Genomic DNA. Translation: CAA97942.1.
BK006949 Genomic DNA. Translation: DAA11209.1.
PIRiS48136.
RefSeqiNP_015097.1. NM_001184041.1.

Genome annotation databases

EnsemblFungiiYPL227C; YPL227C; YPL227C.
GeneIDi855874.
KEGGisce:YPL227C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77573 Genomic DNA. Translation: CAA54680.1.
X94561 Genomic DNA. Translation: CAA64260.1.
Z73583 Genomic DNA. Translation: CAA97942.1.
BK006949 Genomic DNA. Translation: DAA11209.1.
PIRiS48136.
RefSeqiNP_015097.1. NM_001184041.1.

3D structure databases

ProteinModelPortaliP40350.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35958. 151 interactions.
DIPiDIP-5651N.
IntActiP40350. 25 interactions.
MINTiMINT-547084.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Proteomic databases

MaxQBiP40350.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPL227C; YPL227C; YPL227C.
GeneIDi855874.
KEGGisce:YPL227C.

Organism-specific databases

EuPathDBiFungiDB:YPL227C.
SGDiS000006148. ALG5.

Phylogenomic databases

GeneTreeiENSGT00550000074941.
HOGENOMiHOG000283250.
InParanoidiP40350.
KOiK00729.
OMAiFIRLRYM.
OrthoDBiEOG77WWPJ.

Enzyme and pathway databases

UniPathwayiUPA00378.
BioCyciMetaCyc:MONOMER-7202.
YEAST:YPL227C-MONOMER.
ReactomeiR-SCE-480985. Synthesis of dolichyl-phosphate-glucose.

Miscellaneous databases

PROiP40350.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of the ALG5 locus encoding the UDP-glucose:dolichyl-phosphate glucosyltransferase from Saccharomyces cerevisiae."
    Te Heesen S., Lehle L., Weissmann A., Aebi M.
    Eur. J. Biochem. 224:71-79(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiALG5_YEAST
AccessioniPrimary (citable) accession number: P40350
Secondary accession number(s): D6W3E3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 8, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5800 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.