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Protein

Dolichyl-phosphate beta-glucosyltransferase

Gene

ALG5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 5800 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

UDP-glucose + dolichyl phosphate = UDP + dolichyl beta-D-glucosyl phosphate.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • dolichyl-phosphate beta-glucosyltransferase activity Source: SGD

GO - Biological processi

  • protein N-linked glycosylation Source: UniProtKB

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:YPL227C-MONOMER
YEAST:YPL227C-MONOMER
ReactomeiR-SCE-480985 Synthesis of dolichyl-phosphate-glucose
UniPathwayiUPA00378

Protein family/group databases

CAZyiGT2 Glycosyltransferase Family 2

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-phosphate beta-glucosyltransferase (EC:2.4.1.117)
Short name:
DolP-glucosyltransferase
Alternative name(s):
Asparagine-linked glycosylation protein 5
Gene namesi
Name:ALG5
Ordered Locus Names:YPL227C
ORF Names:P1437
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPL227C
SGDiS000006148 ALG5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Transmembranei13 – 33Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini34 – 334LumenalSequence analysisAdd BLAST301

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000590971 – 334Dolichyl-phosphate beta-glucosyltransferaseAdd BLAST334

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi83N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi119N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP40350
PaxDbiP40350
PRIDEiP40350

Interactioni

Protein-protein interaction databases

BioGridi35958248 interactors.
DIPiDIP-5651N
IntActiP40350 31 interactors.
MINTiP40350
STRINGi4932.YPL227C

Structurei

3D structure databases

ProteinModelPortaliP40350
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00550000074941
HOGENOMiHOG000283250
InParanoidiP40350
KOiK00729
OMAiLTPFWSW
OrthoDBiEOG092C4EMU

Family and domain databases

CDDicd04188 DPG_synthase, 1 hit
Gene3Di3.90.550.101 hit
InterProiView protein in InterPro
IPR035518 DPG_synthase
IPR001173 Glyco_trans_2-like
IPR029044 Nucleotide-diphossugar_trans
PfamiView protein in Pfam
PF00535 Glycos_transf_2, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit

Sequencei

Sequence statusi: Complete.

P40350-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRALRFLIEN RNTVFFTLLV ALVLSLYLLV YLFSHTPRPP YPEELKYIAI
60 70 80 90 100
DEKGHEVSRA LPNLNEHQDD EEIFLSVVIP SYNETGRILL MLTDAISFLK
110 120 130 140 150
EKYGSRWEIV IVDDGSTDNT TQYCLKICKE QFKLNYEQFR IIKFSQNRGK
160 170 180 190 200
GGAVRQGFLH IRGKYGLFAD ADGASKFSDV EKLIDAISKI ETSSTDLKTT
210 220 230 240 250
KPAVAIGSRA HMVNTEAVIK RSMIRNCLMY GFHTLVFIFG IRSIKDTQCG
260 270 280 290 300
FKLFNRAAIL KIFPYLHTEG WIFDVEILIL AIRKRIQIEE IPISWHEVDG
310 320 330
SKMALAIDSI KMAKDLVIIR MAYLLGIYRD NKKC
Length:334
Mass (Da):38,347
Last modified:February 1, 1995 - v1
Checksum:i639A88DDED879217
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77573 Genomic DNA Translation: CAA54680.1
X94561 Genomic DNA Translation: CAA64260.1
Z73583 Genomic DNA Translation: CAA97942.1
BK006949 Genomic DNA Translation: DAA11209.1
PIRiS48136
RefSeqiNP_015097.1, NM_001184041.1

Genome annotation databases

EnsemblFungiiYPL227C; YPL227C; YPL227C
GeneIDi855874
KEGGisce:YPL227C

Similar proteinsi

Entry informationi

Entry nameiALG5_YEAST
AccessioniPrimary (citable) accession number: P40350
Secondary accession number(s): D6W3E3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 28, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome