P40350 (ALG5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dolichyl-phosphate beta-glucosyltransferase
Short name=DolP-glucosyltransferase
EC=2.4.1.117

Alternative name(s):
Asparagine-linked glycosylation protein 5

Gene names

Name:	ALG5
Ordered Locus Names:	YPL227C
ORF Names:	P1437

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	UDP-glucose + dolichyl phosphate = UDP + dolichyl beta-D-glucosyl phosphate.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein. Note: Its interaction with the substrate UDP-glucose may occur at the cytoplasmic side of the ER, whereas the steps utilizing dolichyl beta-D-glucosyl phosphate take place in the lumen of the ER.
Miscellaneous	Present with 5800 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the glycosyltransferase 2 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein N-linked glycosylation Traceable author statement Ref.1. Source: SGD
Cellular_component	endoplasmic reticulum membrane Inferred from direct assay PubMed 9560251. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	dolichyl-phosphate beta-glucosyltransferase activity Inferred from direct assay Ref.1. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Dolichyl-phosphate beta-glucosyltransferase		PRO_0000059097
Regions
	Topological domain	1 – 12	12	Cytoplasmic Potential
	Transmembrane	13 – 33	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	34 – 334	301	Lumenal Potential
Amino acid modifications
	Glycosylation	83	1	N-linked (GlcNAc...) Potential
	Glycosylation	119	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P40350 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 639A88DDED879217	FASTA	334	38,347
	10 20 30 40 50 60 MRALRFLIEN RNTVFFTLLV ALVLSLYLLV YLFSHTPRPP YPEELKYIAI DEKGHEVSRA 70 80 90 100 110 120 LPNLNEHQDD EEIFLSVVIP SYNETGRILL MLTDAISFLK EKYGSRWEIV IVDDGSTDNT 130 140 150 160 170 180 TQYCLKICKE QFKLNYEQFR IIKFSQNRGK GGAVRQGFLH IRGKYGLFAD ADGASKFSDV 190 200 210 220 230 240 EKLIDAISKI ETSSTDLKTT KPAVAIGSRA HMVNTEAVIK RSMIRNCLMY GFHTLVFIFG 250 260 270 280 290 300 IRSIKDTQCG FKLFNRAAIL KIFPYLHTEG WIFDVEILIL AIRKRIQIEE IPISWHEVDG 310 320 330 SKMALAIDSI KMAKDLVIIR MAYLLGIYRD NKKC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of the ALG5 locus encoding the UDP-glucose:dolichyl-phosphate glucosyltransferase from Saccharomyces cerevisiae." Te Heesen S., Lehle L., Weissmann A., Aebi M. Eur. J. Biochem. 224:71-79(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X77573 Genomic DNA. Translation: CAA54680.1. X94561 Genomic DNA. Translation: CAA64260.1. Z73583 Genomic DNA. Translation: CAA97942.1. BK006949 Genomic DNA. Translation: DAA11209.1.
PIR	S48136.
RefSeq	NP_015097.1. NM_001184041.1.
3D structure databases
ProteinModelPortal	P40350.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5651N.
IntAct	P40350. 15 interactions.
MINT	MINT-547084.
STRING	4932.YPL227C.
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Proteomic databases
PaxDb	P40350.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL227C; YPL227C; YPL227C.
GeneID	855874.
KEGG	sce:YPL227C.
Organism-specific databases
CYGD	YPL227c.
SGD	S000006148. ALG5.
Phylogenomic databases
eggNOG	COG0463.
GeneTree	ENSGT00550000074941.
HOGENOM	HOG000283250.
KO	K00729.
OMA	GAVRMGV.
OrthoDB	EOG4S7P01.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-7202.
UniPathway	UPA00378.
Gene expression databases
Genevestigator	P40350.
GermOnline	YPL227C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001173. Glyco_trans_2. [Graphical view]
Pfam	PF00535. Glycos_transf_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980514.

Entry information

Entry name

ALG5_YEAST

Accession

Primary (citable) accession number: P40350
Secondary accession number(s): D6W3E3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program