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P40347

- PPAL_YEAST

UniProt

P40347 - PPAL_YEAST

Protein

Low molecular weight phosphotyrosine protein phosphatase

Gene

LTP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 1 (01 Feb 1995)
      Previous versions | rss
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    Functioni

    Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates.By similarity

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
    A phosphate monoester + H2O = an alcohol + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei14 – 141NucleophileBy similarity
    Active sitei20 – 201By similarity
    Active sitei133 – 1331Proton donorBy similarity

    GO - Molecular functioni

    1. acid phosphatase activity Source: UniProtKB-EC
    2. protein tyrosine phosphatase activity Source: SGD

    GO - Biological processi

    1. peptidyl-tyrosine dephosphorylation Source: GOC
    2. protein dephosphorylation Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34220-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Low molecular weight phosphotyrosine protein phosphatase (EC:3.1.3.48)
    Alternative name(s):
    Low molecular weight cytosolic acid phosphatase (EC:3.1.3.2)
    PTPase
    Gene namesi
    Name:LTP1
    Ordered Locus Names:YPR073C
    ORF Names:YP9499.28C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XVI

    Organism-specific databases

    CYGDiYPR073c.
    SGDiS000006277. LTP1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 161161Low molecular weight phosphotyrosine protein phosphatasePRO_0000046565Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei57 – 571Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP40347.
    PaxDbiP40347.
    PeptideAtlasiP40347.

    Expressioni

    Gene expression databases

    GenevestigatoriP40347.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MOT1P323331EBI-13757,EBI-11152

    Protein-protein interaction databases

    BioGridi36246. 23 interactions.
    DIPiDIP-6559N.
    MINTiMINT-689803.
    STRINGi4932.YPR073C.

    Structurei

    Secondary structure

    1
    161
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi8 – 1912
    Helixi20 – 3415
    Helixi38 – 403
    Beta strandi41 – 5010
    Turni52 – 554
    Helixi60 – 689
    Helixi82 – 865
    Beta strandi89 – 957
    Helixi96 – 10510
    Beta strandi113 – 1164
    Helixi117 – 1204
    Beta strandi124 – 1274
    Helixi139 – 16022

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1D1PX-ray2.20A/B2-161[»]
    1D1QX-ray1.70A/B1-161[»]
    1D2AX-ray1.90A/B2-161[»]
    ProteinModelPortaliP40347.
    SMRiP40347. Positions 5-161.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP40347.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0394.
    GeneTreeiENSGT00500000044891.
    HOGENOMiHOG000273094.
    KOiK14394.
    OMAiEIMSADL.
    OrthoDBiEOG7P2Z4K.

    Family and domain databases

    InterProiIPR023485. Ptyr_pPase_SF.
    IPR000106. Tyr_phospatase/Ars_reductase.
    IPR017867. Tyr_phospatase_low_mol_wt.
    [Graphical view]
    PANTHERiPTHR11717. PTHR11717. 1 hit.
    PfamiPF01451. LMWPc. 1 hit.
    [Graphical view]
    PRINTSiPR00719. LMWPTPASE.
    SMARTiSM00226. LMWPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52788. SSF52788. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P40347-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTIEKPKISV AFICLGNFCR SPMAEAIFKH EVEKANLENR FNKIDSFGTS    50
    NYHVGESPDH RTVSICKQHG VKINHKGKQI KTKHFDEYDY IIGMDESNIN 100
    NLKKIQPEGS KAKVCLFGDW NTNDGTVQTI IEDPWYGDIQ DFEYNFKQIT 150
    YFSKQFLKKE L 161
    Length:161
    Mass (Da):18,675
    Last modified:February 1, 1995 - v1
    Checksum:i0911F4B85C050AB3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U11057 mRNA. Translation: AAA99546.1.
    L48604 Genomic DNA. Translation: AAA80146.1.
    Z49219 Genomic DNA. Translation: CAA89190.1.
    Z71255 Genomic DNA. Translation: CAA94981.1.
    U51033 Genomic DNA. Translation: AAB68124.1.
    AY692891 Genomic DNA. Translation: AAT92910.1.
    BK006949 Genomic DNA. Translation: DAA11493.1.
    PIRiA57390.
    RefSeqiNP_015398.1. NM_001184170.1.

    Genome annotation databases

    EnsemblFungiiYPR073C; YPR073C; YPR073C.
    GeneIDi856187.
    KEGGisce:YPR073C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U11057 mRNA. Translation: AAA99546.1 .
    L48604 Genomic DNA. Translation: AAA80146.1 .
    Z49219 Genomic DNA. Translation: CAA89190.1 .
    Z71255 Genomic DNA. Translation: CAA94981.1 .
    U51033 Genomic DNA. Translation: AAB68124.1 .
    AY692891 Genomic DNA. Translation: AAT92910.1 .
    BK006949 Genomic DNA. Translation: DAA11493.1 .
    PIRi A57390.
    RefSeqi NP_015398.1. NM_001184170.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1D1P X-ray 2.20 A/B 2-161 [» ]
    1D1Q X-ray 1.70 A/B 1-161 [» ]
    1D2A X-ray 1.90 A/B 2-161 [» ]
    ProteinModelPortali P40347.
    SMRi P40347. Positions 5-161.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36246. 23 interactions.
    DIPi DIP-6559N.
    MINTi MINT-689803.
    STRINGi 4932.YPR073C.

    Chemistry

    BindingDBi P40347.
    ChEMBLi CHEMBL5397.
    DrugBanki DB00173. Adenine.

    Proteomic databases

    MaxQBi P40347.
    PaxDbi P40347.
    PeptideAtlasi P40347.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPR073C ; YPR073C ; YPR073C .
    GeneIDi 856187.
    KEGGi sce:YPR073C.

    Organism-specific databases

    CYGDi YPR073c.
    SGDi S000006277. LTP1.

    Phylogenomic databases

    eggNOGi COG0394.
    GeneTreei ENSGT00500000044891.
    HOGENOMi HOG000273094.
    KOi K14394.
    OMAi EIMSADL.
    OrthoDBi EOG7P2Z4K.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34220-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P40347.
    NextBioi 981369.
    PROi P40347.

    Gene expression databases

    Genevestigatori P40347.

    Family and domain databases

    InterProi IPR023485. Ptyr_pPase_SF.
    IPR000106. Tyr_phospatase/Ars_reductase.
    IPR017867. Tyr_phospatase_low_mol_wt.
    [Graphical view ]
    PANTHERi PTHR11717. PTHR11717. 1 hit.
    Pfami PF01451. LMWPc. 1 hit.
    [Graphical view ]
    PRINTSi PR00719. LMWPTPASE.
    SMARTi SM00226. LMWPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52788. SSF52788. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of a Saccharomyces cerevisiae gene encoding the low molecular weight protein-tyrosine phosphatase."
      Ostanin K., Pokalsky C., Wang S., van Etten R.L.
      J. Biol. Chem. 270:18491-18499(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: Y133.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-57, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPPAL_YEAST
    AccessioniPrimary (citable) accession number: P40347
    Secondary accession number(s): D6W477
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 132 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 3500 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3