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P40347 (PPAL_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Low molecular weight phosphotyrosine protein phosphatase

EC=3.1.3.48
Alternative name(s):
Low molecular weight cytosolic acid phosphatase
EC=3.1.3.2
PTPase
Gene names
Name:LTP1
Ordered Locus Names:YPR073C
ORF Names:YP9499.28C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length161 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates By similarity.

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

A phosphate monoester + H2O = an alcohol + phosphate.

Subcellular location

Cytoplasm.

Miscellaneous

Present with 3500 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the low molecular weight phosphotyrosine protein phosphatase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MOT1P323331EBI-13757,EBI-11152

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 161161Low molecular weight phosphotyrosine protein phosphatase
PRO_0000046565

Sites

Active site141Nucleophile By similarity
Active site201 By similarity
Active site1331Proton donor By similarity

Amino acid modifications

Modified residue571Phosphoserine Ref.6

Secondary structure

....................... 161
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P40347 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 0911F4B85C050AB3

FASTA16118,675
        10         20         30         40         50         60 
MTIEKPKISV AFICLGNFCR SPMAEAIFKH EVEKANLENR FNKIDSFGTS NYHVGESPDH 

        70         80         90        100        110        120 
RTVSICKQHG VKINHKGKQI KTKHFDEYDY IIGMDESNIN NLKKIQPEGS KAKVCLFGDW 

       130        140        150        160 
NTNDGTVQTI IEDPWYGDIQ DFEYNFKQIT YFSKQFLKKE L 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of a Saccharomyces cerevisiae gene encoding the low molecular weight protein-tyrosine phosphatase."
Ostanin K., Pokalsky C., Wang S., van Etten R.L.
J. Biol. Chem. 270:18491-18499(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: Y133.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. expand/collapse author list , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-57, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U11057 mRNA. Translation: AAA99546.1.
L48604 Genomic DNA. Translation: AAA80146.1.
Z49219 Genomic DNA. Translation: CAA89190.1.
Z71255 Genomic DNA. Translation: CAA94981.1.
U51033 Genomic DNA. Translation: AAB68124.1.
AY692891 Genomic DNA. Translation: AAT92910.1.
BK006949 Genomic DNA. Translation: DAA11493.1.
PIRA57390.
RefSeqNP_015398.1. NM_001184170.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1D1PX-ray2.20A/B2-161[»]
1D1QX-ray1.70A/B1-161[»]
1D2AX-ray1.90A/B2-161[»]
ProteinModelPortalP40347.
SMRP40347. Positions 5-161.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid36246. 23 interactions.
DIPDIP-6559N.
MINTMINT-689803.
STRING4932.YPR073C.

Chemistry

BindingDBP40347.
ChEMBLCHEMBL5397.
DrugBankDB00173. Adenine.

Proteomic databases

MaxQBP40347.
PaxDbP40347.
PeptideAtlasP40347.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYPR073C; YPR073C; YPR073C.
GeneID856187.
KEGGsce:YPR073C.

Organism-specific databases

CYGDYPR073c.
SGDS000006277. LTP1.

Phylogenomic databases

eggNOGCOG0394.
GeneTreeENSGT00500000044891.
HOGENOMHOG000273094.
KOK14394.
OMAEIMSADL.
OrthoDBEOG7P2Z4K.

Enzyme and pathway databases

BioCycYEAST:G3O-34220-MONOMER.

Gene expression databases

GenevestigatorP40347.

Family and domain databases

InterProIPR023485. Ptyr_pPase_SF.
IPR000106. Tyr_phospatase/Ars_reductase.
IPR017867. Tyr_phospatase_low_mol_wt.
[Graphical view]
PANTHERPTHR11717. PTHR11717. 1 hit.
PfamPF01451. LMWPc. 1 hit.
[Graphical view]
PRINTSPR00719. LMWPTPASE.
SMARTSM00226. LMWPc. 1 hit.
[Graphical view]
SUPFAMSSF52788. SSF52788. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP40347.
NextBio981369.
PROP40347.

Entry information

Entry namePPAL_YEAST
AccessionPrimary (citable) accession number: P40347
Secondary accession number(s): D6W477
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 14, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XVI

Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references