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Protein

Low molecular weight phosphotyrosine protein phosphatase

Gene

LTP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates.By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
A phosphate monoester + H2O = an alcohol + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei14 – 141NucleophileBy similarity
Active sitei20 – 201By similarity
Active sitei133 – 1331Proton donorBy similarity

GO - Molecular functioni

  • acid phosphatase activity Source: UniProtKB-EC
  • protein tyrosine phosphatase activity Source: SGD

GO - Biological processi

  • peptidyl-tyrosine dephosphorylation Source: GOC
  • protein dephosphorylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Enzyme and pathway databases

BioCyciYEAST:G3O-34220-MONOMER.
BRENDAi3.1.3.48. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Low molecular weight phosphotyrosine protein phosphatase (EC:3.1.3.48)
Alternative name(s):
Low molecular weight cytosolic acid phosphatase (EC:3.1.3.2)
PTPase
Gene namesi
Name:LTP1
Ordered Locus Names:YPR073C
ORF Names:YP9499.28C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XVI

Organism-specific databases

CYGDiYPR073c.
EuPathDBiFungiDB:YPR073C.
SGDiS000006277. LTP1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 161161Low molecular weight phosphotyrosine protein phosphatasePRO_0000046565Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei57 – 571Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40347.
PaxDbiP40347.
PeptideAtlasiP40347.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
MOT1P323331EBI-13757,EBI-11152

Protein-protein interaction databases

BioGridi36246. 22 interactions.
DIPiDIP-6559N.
MINTiMINT-689803.

Structurei

Secondary structure

1
161
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 1912Combined sources
Helixi20 – 3415Combined sources
Helixi38 – 403Combined sources
Beta strandi41 – 5010Combined sources
Turni52 – 554Combined sources
Helixi60 – 689Combined sources
Helixi82 – 865Combined sources
Beta strandi89 – 957Combined sources
Helixi96 – 10510Combined sources
Beta strandi113 – 1164Combined sources
Helixi117 – 1204Combined sources
Beta strandi124 – 1274Combined sources
Helixi139 – 16022Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1D1PX-ray2.20A/B2-161[»]
1D1QX-ray1.70A/B1-161[»]
1D2AX-ray1.90A/B2-161[»]
ProteinModelPortaliP40347.
SMRiP40347. Positions 5-161.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40347.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0394.
GeneTreeiENSGT00500000044891.
HOGENOMiHOG000273094.
InParanoidiP40347.
KOiK14394.
OMAiENKWRID.
OrthoDBiEOG7P2Z4K.

Family and domain databases

InterProiIPR023485. Ptyr_pPase_SF.
IPR017867. Tyr_phospatase_low_mol_wt.
[Graphical view]
PANTHERiPTHR11717:SF7. PTHR11717:SF7. 1 hit.
PfamiPF01451. LMWPc. 1 hit.
[Graphical view]
PRINTSiPR00719. LMWPTPASE.
SMARTiSM00226. LMWPc. 1 hit.
[Graphical view]
SUPFAMiSSF52788. SSF52788. 1 hit.

Sequencei

Sequence statusi: Complete.

P40347-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIEKPKISV AFICLGNFCR SPMAEAIFKH EVEKANLENR FNKIDSFGTS
60 70 80 90 100
NYHVGESPDH RTVSICKQHG VKINHKGKQI KTKHFDEYDY IIGMDESNIN
110 120 130 140 150
NLKKIQPEGS KAKVCLFGDW NTNDGTVQTI IEDPWYGDIQ DFEYNFKQIT
160
YFSKQFLKKE L
Length:161
Mass (Da):18,675
Last modified:February 1, 1995 - v1
Checksum:i0911F4B85C050AB3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11057 mRNA. Translation: AAA99546.1.
L48604 Genomic DNA. Translation: AAA80146.1.
Z49219 Genomic DNA. Translation: CAA89190.1.
Z71255 Genomic DNA. Translation: CAA94981.1.
U51033 Genomic DNA. Translation: AAB68124.1.
AY692891 Genomic DNA. Translation: AAT92910.1.
BK006949 Genomic DNA. Translation: DAA11493.1.
PIRiA57390.
RefSeqiNP_015398.1. NM_001184170.1.

Genome annotation databases

EnsemblFungiiYPR073C; YPR073C; YPR073C.
GeneIDi856187.
KEGGisce:YPR073C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U11057 mRNA. Translation: AAA99546.1.
L48604 Genomic DNA. Translation: AAA80146.1.
Z49219 Genomic DNA. Translation: CAA89190.1.
Z71255 Genomic DNA. Translation: CAA94981.1.
U51033 Genomic DNA. Translation: AAB68124.1.
AY692891 Genomic DNA. Translation: AAT92910.1.
BK006949 Genomic DNA. Translation: DAA11493.1.
PIRiA57390.
RefSeqiNP_015398.1. NM_001184170.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1D1PX-ray2.20A/B2-161[»]
1D1QX-ray1.70A/B1-161[»]
1D2AX-ray1.90A/B2-161[»]
ProteinModelPortaliP40347.
SMRiP40347. Positions 5-161.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36246. 22 interactions.
DIPiDIP-6559N.
MINTiMINT-689803.

Chemistry

BindingDBiP40347.
ChEMBLiCHEMBL5397.

Proteomic databases

MaxQBiP40347.
PaxDbiP40347.
PeptideAtlasiP40347.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR073C; YPR073C; YPR073C.
GeneIDi856187.
KEGGisce:YPR073C.

Organism-specific databases

CYGDiYPR073c.
EuPathDBiFungiDB:YPR073C.
SGDiS000006277. LTP1.

Phylogenomic databases

eggNOGiCOG0394.
GeneTreeiENSGT00500000044891.
HOGENOMiHOG000273094.
InParanoidiP40347.
KOiK14394.
OMAiENKWRID.
OrthoDBiEOG7P2Z4K.

Enzyme and pathway databases

BioCyciYEAST:G3O-34220-MONOMER.
BRENDAi3.1.3.48. 984.

Miscellaneous databases

EvolutionaryTraceiP40347.
NextBioi981369.
PROiP40347.

Family and domain databases

InterProiIPR023485. Ptyr_pPase_SF.
IPR017867. Tyr_phospatase_low_mol_wt.
[Graphical view]
PANTHERiPTHR11717:SF7. PTHR11717:SF7. 1 hit.
PfamiPF01451. LMWPc. 1 hit.
[Graphical view]
PRINTSiPR00719. LMWPTPASE.
SMARTiSM00226. LMWPc. 1 hit.
[Graphical view]
SUPFAMiSSF52788. SSF52788. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of a Saccharomyces cerevisiae gene encoding the low molecular weight protein-tyrosine phosphatase."
    Ostanin K., Pokalsky C., Wang S., van Etten R.L.
    J. Biol. Chem. 270:18491-18499(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: Y133.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
    Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
    , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
    Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-57, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPPAL_YEAST
AccessioniPrimary (citable) accession number: P40347
Secondary accession number(s): D6W477
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 22, 2015
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.