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P40344

- ATG3_YEAST

UniProt

P40344 - ATG3_YEAST

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Protein

Autophagy-related protein 3

Gene

ATG3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt) and autophagy. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8. The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8 from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8. The formation of the ATG8-phosphatidylethanolamine conjugate is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). The ATG8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy.21 Publications

Enzyme regulationi

ATG12-ATG5 induces reorientation of the ATG3 structure, increasing conjugation activity of ATG3.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei234 – 2341Glycyl thioester intermediateSequence Analysis

GO - Molecular functioni

  1. Atg8 ligase activity Source: SGD

GO - Biological processi

  1. autophagic vacuole assembly Source: UniProtKB
  2. C-terminal protein lipidation Source: SGD
  3. CVT pathway Source: SGD
  4. late nucleophagy Source: SGD
  5. mitochondrion degradation Source: SGD
  6. piecemeal microautophagy of nucleus Source: SGD
  7. protein targeting to membrane Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Autophagy, Protein transport, Transport, Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-33325-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Autophagy-related protein 3
Alternative name(s):
Autophagy-related E2-like conjugation enzyme ATG3
Gene namesi
Name:ATG3
Synonyms:APG3, AUT1
Ordered Locus Names:YNR007C
ORF Names:N2040
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNR007c.
SGDiS000005290. ATG3.

Subcellular locationi

Cytoplasm 2 Publications

GO - Cellular componenti

  1. cytosol Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi213 – 2131T → A: Decreases ATG8-PE comjugation and autophagy. 1 Publication
Mutagenesisi234 – 2341C → A: Loss of interaction with ATG8 and defect in autophagy and Cvt pathway. 2 Publications
Mutagenesisi234 – 2341C → S: Instead of the formation of an intermediate complex with a thiol ester bond between ATG3 (E2-like enzyme) and ATG8 (substrate), a stable complex with an O-ester bond is formed. 2 Publications
Mutagenesisi270 – 2701W → A: Decreases interaction with ATG8. 1 Publication
Mutagenesisi272 – 2721D → A: Decreases interaction with ATG8. 1 Publication
Mutagenesisi273 – 2731L → A: Decreases interaction with ATG8. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 310310Autophagy-related protein 3PRO_0000213586Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei19 – 191N6-acetyllysine; by ESA11 Publication
Modified residuei48 – 481N6-acetyllysine; by ESA11 Publication

Post-translational modificationi

Acetylated by NuA4 complex acetyltransferase ESA1 at Lys-19 and Lys-48. Acetylation regulates autophagy by controlling ATG8 interaction and lipidation. Deacetylated by histone deacetylase RPD3.2 Publications

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP40344.
PaxDbiP40344.
PeptideAtlasiP40344.

Expressioni

Inductioni

Expression is increased during filamentous growth and controlled by the RSF2 transcription factor.2 Publications

Gene expression databases

GenevestigatoriP40344.

Interactioni

Subunit structurei

Monomer. Interacts with ATG8 through an intermediate thioester bond between Cys-234 and the C-terminal Gly of ATG8. Also interacts with the 40 amino acid C-terminal region of the E1-like ATG7 enzyme. Interacts also with the ATG12-ATG5 conjugate.13 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ATG7P388624EBI-3381,EBI-2677
ATG8P381823EBI-3381,EBI-2684

Protein-protein interaction databases

BioGridi35832. 100 interactions.
DIPiDIP-1190N.
IntActiP40344. 10 interactions.
MINTiMINT-389035.
STRINGi4932.YNR007C.

Structurei

Secondary structure

1
310
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi22 – 254Combined sources
Helixi30 – 4314Combined sources
Beta strandi47 – 493Combined sources
Helixi64 – 663Combined sources
Beta strandi69 – 768Combined sources
Turni80 – 823Combined sources
Helixi135 – 1384Combined sources
Beta strandi167 – 17610Combined sources
Turni177 – 1804Combined sources
Beta strandi181 – 19010Combined sources
Helixi198 – 2025Combined sources
Turni207 – 2093Combined sources
Helixi210 – 2134Combined sources
Beta strandi214 – 2185Combined sources
Beta strandi222 – 2243Combined sources
Beta strandi227 – 2315Combined sources
Beta strandi235 – 2373Combined sources
Helixi239 – 26426Combined sources
Helixi283 – 2853Combined sources
Helixi286 – 29712Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DYTX-ray2.50A1-310[»]
3T7GX-ray2.08C/D128-144[»]
4GSLX-ray2.70C/D1-310[»]
ProteinModelPortaliP40344.
SMRiP40344. Positions 21-305.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40344.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 77PE-binding
Regioni83 – 16381Flexible regionAdd
BLAST
Regioni238 – 28548Handle regionAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi270 – 2734ATG8 interaction motif (AIM)

Domaini

The N-teminal region (residues 1-7) is involved in phosphatidylethanolamine-binding and is required for ATG8-PE conjugation.
The flexible region (FR) is required for ATG7-binding.
The handle region (HR) contains the ATG8 interaction motif (AIM) and mediates binding to ATG8. It is crucial for the cytoplasm-to-vacuole targeting pathway.

Sequence similaritiesi

Belongs to the ATG3 family.Curated

Phylogenomic databases

eggNOGiNOG237080.
GeneTreeiENSGT00390000010308.
HOGENOMiHOG000234613.
InParanoidiP40344.
KOiK08343.
OMAiNEQQGHA.
OrthoDBiEOG7KWSTW.

Family and domain databases

InterProiIPR007135. Autophagy-rel_prot_3.
IPR019461. Autophagy-rel_prot_3_C.
IPR007134. Autophagy-rel_prot_3_N.
[Graphical view]
PfamiPF03987. Autophagy_act_C. 1 hit.
PF10381. Autophagy_Cterm. 1 hit.
PF03986. Autophagy_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40344-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIRSTLSSWR EYLTPITHKS TFLTTGQITP EEFVQAGDYL CHMFPTWKWN
60 70 80 90 100
EESSDISYRD FLPKNKQFLI IRKVPCDKRA EQCVEVEGPD VIMKGFAEDG
110 120 130 140 150
DEDDVLEYIG SETEHVQSTP AGGTKDSSID DIDELIQDME IKEEDENDDT
160 170 180 190 200
EEFNAKGGLA KDMAQERYYD LYIAYSTSYR VPKMYIVGFN SNGSPLSPEQ
210 220 230 240 250
MFEDISADYR TKTATIEKLP FYKNSVLSVS IHPCKHANVM KILLDKVRVV
260 270 280 290 300
RQRRRKELQE EQELDGVGDW EDLQDDIDDS LRVDQYLIVF LKFITSVTPS
310
IQHDYTMEGW
Length:310
Mass (Da):35,887
Last modified:February 1, 1995 - v1
Checksum:i52CCFB216B18CF0C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77395 Genomic DNA. Translation: CAA54575.1.
Z71622 Genomic DNA. Translation: CAA96284.1.
BK006947 Genomic DNA. Translation: DAA10548.1.
PIRiS45130.
RefSeqiNP_014404.3. NM_001183184.3.

Genome annotation databases

EnsemblFungiiYNR007C; YNR007C; YNR007C.
GeneIDi855741.
KEGGisce:YNR007C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77395 Genomic DNA. Translation: CAA54575.1 .
Z71622 Genomic DNA. Translation: CAA96284.1 .
BK006947 Genomic DNA. Translation: DAA10548.1 .
PIRi S45130.
RefSeqi NP_014404.3. NM_001183184.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2DYT X-ray 2.50 A 1-310 [» ]
3T7G X-ray 2.08 C/D 128-144 [» ]
4GSL X-ray 2.70 C/D 1-310 [» ]
ProteinModelPortali P40344.
SMRi P40344. Positions 21-305.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35832. 100 interactions.
DIPi DIP-1190N.
IntActi P40344. 10 interactions.
MINTi MINT-389035.
STRINGi 4932.YNR007C.

Proteomic databases

MaxQBi P40344.
PaxDbi P40344.
PeptideAtlasi P40344.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNR007C ; YNR007C ; YNR007C .
GeneIDi 855741.
KEGGi sce:YNR007C.

Organism-specific databases

CYGDi YNR007c.
SGDi S000005290. ATG3.

Phylogenomic databases

eggNOGi NOG237080.
GeneTreei ENSGT00390000010308.
HOGENOMi HOG000234613.
InParanoidi P40344.
KOi K08343.
OMAi NEQQGHA.
OrthoDBi EOG7KWSTW.

Enzyme and pathway databases

BioCyci YEAST:G3O-33325-MONOMER.

Miscellaneous databases

EvolutionaryTracei P40344.
NextBioi 980136.
PROi P40344.

Gene expression databases

Genevestigatori P40344.

Family and domain databases

InterProi IPR007135. Autophagy-rel_prot_3.
IPR019461. Autophagy-rel_prot_3_C.
IPR007134. Autophagy-rel_prot_3_N.
[Graphical view ]
Pfami PF03987. Autophagy_act_C. 1 hit.
PF10381. Autophagy_Cterm. 1 hit.
PF03986. Autophagy_N. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "AUT1, a gene essential for autophagocytosis in the yeast Saccharomyces cerevisiae."
    Schlumpberger M., Schaeffeler E., Straub M., Bredschneider M., Wolf D.H., Thumm M.
    J. Bacteriol. 179:1068-1076(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
  2. "Twelve open reading frames revealed in the 23.6 kb segment flanking the centromere on the Saccharomyces cerevisiae chromosome XIV right arm."
    Verhasselt P., Aert R., Voet M., Volckaert G.
    Yeast 10:1355-1361(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Isolation and characterization of autophagy-defective mutants of Saccharomyces cerevisiae."
    Tsukada M., Ohsumi Y.
    FEBS Lett. 333:169-174(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Isolation of autophagocytosis mutants of Saccharomyces cerevisiae."
    Thumm M., Egner R., Koch B., Schlumpberger M., Straub M., Veenhuis M., Wolf D.H.
    FEBS Lett. 349:275-280(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "The breakdown of autophagic vesicles inside the vacuole depends on Aut4p."
    Suriapranata I., Epple U.D., Bernreuther D., Bredschneider M., Sovarasteanu K., Thumm M.
    J. Cell Sci. 113:4025-4033(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: FUNCTION, INTERACTION WITH ATG8, MUTAGENESIS OF CYS-234.
  9. "The pre-autophagosomal structure organized by concerted functions of APG genes is essential for autophagosome formation."
    Suzuki K., Kirisako T., Kamada Y., Mizushima N., Noda T., Ohsumi Y.
    EMBO J. 20:5971-5981(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "The C-terminal region of an Apg7p/Cvt2p is required for homodimerization and is essential for its E1 activity and E1-E2 complex formation."
    Komatsu M., Tanida I., Ueno T., Ohsumi M., Ohsumi Y., Kominami E.
    J. Biol. Chem. 276:9846-9854(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ATG7.
  11. "Membrane recruitment of Aut7p in the autophagy and cytoplasm to vacuole targeting pathways requires Aut1p, Aut2p, and the autophagy conjugation complex."
    Kim J., Huang W.-P., Klionsky D.J.
    J. Cell Biol. 152:51-64(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  12. "The carboxyl terminal 17 amino acids within Apg7 are essential for Apg8 lipidation, but not for Apg12 conjugation."
    Yamazaki-Sato H., Tanida I., Ueno T., Kominami E.
    FEBS Lett. 551:71-77(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ATG7.
  13. Cited for: NOMENCLATURE.
  14. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  15. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  16. "In vivo and in vitro reconstitution of atg8 conjugation essential for autophagy."
    Ichimura Y., Imamura Y., Emoto K., Umeda M., Noda T., Ohsumi Y.
    J. Biol. Chem. 279:40584-40592(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF CYS-234.
  17. Cited for: CRYSTALLIZATION.
  18. "Atg8, a ubiquitin-like protein required for autophagosome formation, mediates membrane tethering and hemifusion."
    Nakatogawa H., Ichimura Y., Ohsumi Y.
    Cell 130:165-178(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  19. "An interrelationship between autophagy and filamentous growth in budding yeast."
    Ma J., Jin R., Jia X., Dobry C.J., Wang L., Reggiori F., Zhu J., Kumar A.
    Genetics 177:205-214(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  20. "The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy."
    Hanada T., Noda N.N., Satomi Y., Ichimura Y., Fujioka Y., Takao T., Inagaki F., Ohsumi Y.
    J. Biol. Chem. 282:37298-37302(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH THE ATG12-ATG5 CONJUGATE.
  21. "Physiological pH and acidic phospholipids contribute to substrate specificity in lipidation of Atg8."
    Oh-oka K., Nakatogawa H., Ohsumi Y.
    J. Biol. Chem. 283:21847-21852(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  22. "In vivo reconstitution of autophagy in Saccharomyces cerevisiae."
    Cao Y., Cheong H., Song H., Klionsky D.J.
    J. Cell Biol. 182:703-713(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  23. "Piecemeal microautophagy of the nucleus requires the core macroautophagy genes."
    Krick R., Muehe Y., Prick T., Bremer S., Schlotterhose P., Eskelinen E.L., Millen J., Goldfarb D.S., Thumm M.
    Mol. Biol. Cell 19:4492-4505(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  24. "Protein acetylation microarray reveals that NuA4 controls key metabolic target regulating gluconeogenesis."
    Lin Y.Y., Lu J.Y., Zhang J., Walter W., Dang W., Wan J., Tao S.C., Qian J., Zhao Y., Boeke J.D., Berger S.L., Zhu H.
    Cell 136:1073-1084(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION BY THE NUA4 HISTONE ACETYLTRANSFERASE COMPLEX.
  25. "The amino-terminal region of Atg3 is essential for association with phosphatidylethanolamine in Atg8 lipidation."
    Hanada T., Satomi Y., Takao T., Ohsumi Y.
    FEBS Lett. 583:1078-1083(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ATG8, LIPIDATION, DOMAIN.
  26. "Rsf1p is required for an efficient metabolic shift from fermentative to glycerol-based respiratory growth in S. cerevisiae."
    Roberts G.G. III, Hudson A.P.
    Yeast 26:95-110(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  27. "Autophagy-related protein 8 (Atg8) family interacting motif in Atg3 mediates the Atg3-Atg8 interaction and is crucial for the cytoplasm-to-vacuole targeting pathway."
    Yamaguchi M., Noda N.N., Nakatogawa H., Kumeta H., Ohsumi Y., Inagaki F.
    J. Biol. Chem. 285:29599-29607(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ATG8, DOMAIN, MUTAGENESIS OF TRP-270; ASP-272 AND LEU-273.
  28. "Purification and characterization of a ubiquitin-like system for autophagosome formation."
    Bae J.Y., Park H.H.
    J. Microbiol. Biotechnol. 20:1647-1652(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  29. "Structural basis of Atg8 activation by a homodimeric E1, Atg7."
    Noda N.N., Satoo K., Fujioka Y., Kumeta H., Ogura K., Nakatogawa H., Ohsumi Y., Inagaki F.
    Mol. Cell 44:462-475(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ATG7 AND ATG8.
  30. "Insights into noncanonical E1 enzyme activation from the structure of autophagic E1 Atg7 with Atg8."
    Hong S.B., Kim B.W., Lee K.E., Kim S.W., Jeon H., Kim J., Song H.K.
    Nat. Struct. Mol. Biol. 18:1323-1330(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ATG7.
  31. "Atg4 recycles inappropriately lipidated Atg8 to promote autophagosome biogenesis."
    Nakatogawa H., Ishii J., Asai E., Ohsumi Y.
    Autophagy 8:177-186(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  32. "A late form of nucleophagy in Saccharomyces cerevisiae."
    Mijaljica D., Prescott M., Devenish R.J.
    PLoS ONE 7:E40013-E40013(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  33. "Function and molecular mechanism of acetylation in autophagy regulation."
    Yi C., Ma M., Ran L., Zheng J., Tong J., Zhu J., Ma C., Sun Y., Zhang S., Feng W., Zhu L., Le Y., Gong X., Yan X., Hong B., Jiang F.J., Xie Z., Miao D., Deng H., Yu L.
    Science 336:474-477(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION AT LYS-19 AND LYS-48, DEACETYLATION BY RPD3, FUNCTION.
  34. "Atg12-Atg5 conjugate enhances E2 activity of Atg3 by rearranging its catalytic site."
    Sakoh-Nakatogawa M., Matoba K., Asai E., Kirisako H., Ishii J., Noda N.N., Inagaki F., Nakatogawa H., Ohsumi Y.
    Nat. Struct. Mol. Biol. 20:433-439(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF THR-213, INTERACTION WITH THE ATG12-ATG5 CONJUGATE, ENZYME REGULATION.
  35. "The crystal structure of Atg3, an autophagy-related ubiquitin carrier protein (E2) enzyme that mediates Atg8 lipidation."
    Yamada Y., Suzuki N.N., Hanada T., Ichimura Y., Kumeta H., Fujioka Y., Ohsumi Y., Inagaki F.
    J. Biol. Chem. 282:8036-8043(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS), FUNCTION, DOMAIN, INTERACTION WITH ATG7 AND ATG8.
  36. "Atg8 transfer from Atg7 to Atg3: a distinctive E1-E2 architecture and mechanism in the autophagy pathway."
    Taherbhoy A.M., Tait S.W., Kaiser S.E., Williams A.H., Deng A., Nourse A., Hammel M., Kurinov I., Rock C.O., Green D.R., Schulman B.A.
    Mol. Cell 44:451-461(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.08 ANGSTROMS) OF 128-144 IN COMPLEX WITH ATG7.
  37. "Noncanonical E2 recruitment by the autophagy E1 revealed by Atg7-Atg3 and Atg7-Atg10 structures."
    Kaiser S.E., Mao K., Taherbhoy A.M., Yu S., Olszewski J.L., Duda D.M., Kurinov I., Deng A., Fenn T.D., Klionsky D.J., Schulman B.A.
    Nat. Struct. Mol. Biol. 19:1242-1249(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) IN COMPLEX WITH ATG7.

Entry informationi

Entry nameiATG3_YEAST
AccessioniPrimary (citable) accession number: P40344
Secondary accession number(s): D6W1I2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 26, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 3610 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

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