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Protein

Vacuolar protein sorting-associated protein 27

Gene

VPS27

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ESCRT-0 complex which is the sorting receptor for ubiquitinated cargo proteins at the multivesicular body (MVB) and recruits ESCRT-I to the MVB outer membrane. Controls exit from the prevacuolar compartment (PVC) in both the forward direction to the vacuole and the return to the Golgi. Allows VPS10 to return to the (trans-Golgi network) TGN from the PVC. Might also function as an alternate adapter in the COPIb clathrin-like coat.16 Publications

Miscellaneous

Present with 172 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri170 – 230FYVE-typePROSITE-ProRule annotationAdd BLAST61

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • phosphatidylinositol-3-phosphate binding Source: SGD
  • protein domain specific binding Source: CAFA
  • protein heterodimerization activity Source: CAFA
  • ubiquitin binding Source: SGD

GO - Biological processi

  • ATP export Source: SGD
  • late endosome to vacuole transport Source: SGD
  • multivesicular body sorting pathway Source: CAFA
  • positive regulation of protein maturation Source: CAFA
  • protein retention in Golgi apparatus Source: SGD
  • protein secretion Source: CAFA
  • protein targeting to vacuole Source: SGD

Keywordsi

LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-33324-MONOMER
ReactomeiR-SCE-432720 Lysosome Vesicle Biogenesis
R-SCE-5689880 Ub-specific processing proteases

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 27
Alternative name(s):
Golgi retention defective protein 11
Gene namesi
Name:VPS27
Synonyms:DID7, GRD11, SSV17, VPL23, VPT27
Ordered Locus Names:YNR006W
ORF Names:N2038
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNR006W
SGDiS000005289 VPS27

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi185 – 186LL → AA: Decreases the association to PtdIns(3)P containing membranes. 1 Publication2
Mutagenesisi193R → A: Decreases the association to PtdIns(3)P containing membranes. 1 Publication1
Mutagenesisi270S → D: Reduces strongly the ubiquitin-binding activity. 1 Publication1
Mutagenesisi313S → D: Reduces strongly the ubiquitin-binding activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000658931 – 622Vacuolar protein sorting-associated protein 27Add BLAST622

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei157PhosphoserineCombined sources1
Cross-linki294Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei495PhosphoserineCombined sources1
Modified residuei613PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP40343
PaxDbiP40343
PRIDEiP40343

PTM databases

iPTMnetiP40343

Interactioni

Subunit structurei

Component of the ESCRT-0 complex composed of HSE1 and VPS27. Interacts with ENT3 and ENT5, the ESCRT-I subunits VPS23 and VPS28 and with the COPIb subunits SEC27, SEC28 and SEC33.8 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein domain specific binding Source: CAFA
  • protein heterodimerization activity Source: CAFA
  • ubiquitin binding Source: SGD

Protein-protein interaction databases

BioGridi35831, 751 interactors
ComplexPortaliCPX-1622 ESCRT-0 complex
DIPiDIP-1741N
IntActiP40343, 7 interactors
MINTiP40343
STRINGi4932.YNR006W

Structurei

Secondary structure

1622
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni177 – 179Combined sources3
Turni193 – 195Combined sources3
Helixi201 – 203Combined sources3
Beta strandi206 – 210Combined sources5
Helixi211 – 213Combined sources3
Beta strandi215 – 221Combined sources7
Helixi223 – 229Combined sources7
Beta strandi251 – 253Combined sources3
Helixi256 – 269Combined sources14
Helixi274 – 276Combined sources3
Helixi304 – 318Combined sources15
Helixi352 – 369Combined sources18
Turni375 – 379Combined sources5
Helixi381 – 436Combined sources56

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1O06X-ray1.45A301-320[»]
1Q0VNMR-A249-329[»]
1Q0WNMR-A255-278[»]
1VFYX-ray1.15A163-230[»]
2KDINMR-A258-277[»]
2PJWX-ray3.01V348-438[»]
3R42X-ray1.87B445-453[»]
5UCLX-ray2.35B255-278[»]
ProteinModelPortaliP40343
SMRiP40343
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP40343

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 149VHSPROSITE-ProRule annotationAdd BLAST132
Domaini258 – 277UIM 1PROSITE-ProRule annotationAdd BLAST20
Domaini301 – 320UIM 2PROSITE-ProRule annotationAdd BLAST20

Domaini

The FYVE domain is involved in the binding to phosphatidylinositol 3-phosphate (PtdIns3P) which is required for the association to endosomal membranes.
Both IUM domains are necessary for efficient binding to ubiquitin.

Sequence similaritiesi

Belongs to the VPS27 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri170 – 230FYVE-typePROSITE-ProRule annotationAdd BLAST61

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00920000149084
HOGENOMiHOG000000859
InParanoidiP40343
KOiK12182
OMAiPSPIMRR
OrthoDBiEOG092C214M

Family and domain databases

Gene3Di1.25.40.90, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR008942 ENTH_VHS
IPR017073 Ubi-bd_Hrs_VPS27
IPR003903 UIM_dom
IPR002014 VHS_dom
IPR000306 Znf_FYVE
IPR017455 Znf_FYVE-rel
IPR011011 Znf_FYVE_PHD
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF01363 FYVE, 1 hit
PF02809 UIM, 2 hits
PF00790 VHS, 1 hit
PIRSFiPIRSF036956 Hrs_Vps27, 1 hit
SMARTiView protein in SMART
SM00064 FYVE, 1 hit
SM00726 UIM, 2 hits
SM00288 VHS, 1 hit
SUPFAMiSSF48464 SSF48464, 1 hit
SSF57903 SSF57903, 1 hit
PROSITEiView protein in PROSITE
PS50330 UIM, 2 hits
PS50179 VHS, 1 hit
PS50178 ZF_FYVE, 1 hit

Sequencei

Sequence statusi: Complete.

P40343-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVSTPSELD ALIEQATSES IPNGDLDLPI ALEISDVLRS RRVNPKDSMR
60 70 80 90 100
CIKKRILNTA DNPNTQLSSW KLTNICVKNG GTPFIKEICS REFMDTMEHV
110 120 130 140 150
ILREDSNEEL SELVKTILYE LYVAFKNDSQ LNYVAKVYDK LISRGIKFPE
160 170 180 190 200
KLTLSNSPTA MFDSKTPADW IDSDACMICS KKFSLLNRKH HCRSCGGVFC
210 220 230 240 250
QEHSSNSIPL PDLGIYEPVR VCDSCFEDYD LKRHDDSKKS KKHRHKRKKD
260 270 280 290 300
RDYSTPEDEE ELIRKAIELS LKESRNSASS EPIVPVVESK NEVKRQEIEE
310 320 330 340 350
EEDPDLKAAI QESLREAEEA KLRSERQKAS RQMQPQQPSP QPQPIHSVDL
360 370 380 390 400
SDEEKDSIYM FASLVEKMKS RPLNEILEDS KLQNLAQRVF ASKARLNYAL
410 420 430 440 450
NDKAQKYNTL IEMNGKISEI MNIYDRLLEQ QLQSINLSQQ YTLPQVPSDP
460 470 480 490 500
YNYLTENVQN PAESYQTPPL QQLSSHQYKP QQDVSRQQSV KANSSPTTNI
510 520 530 540 550
DHLKTIDVTP HAQQKPQSHV ELAPSDPPYP KEEAEDEGTQ AVQDEESSTQ
560 570 580 590 600
ESRERPYPVE TENGETSINK RPQGITRYDF PTVPARKFVQ PESTVPLPAS
610 620
SSEIPIKEER PPSPQEELLI EL
Length:622
Mass (Da):70,974
Last modified:October 5, 2010 - v3
Checksum:i76C4501B0EBC9C84
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti321 – 322KL → NV in AAA96002 (PubMed:7593183).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24218 Genomic DNA Translation: AAA96002.1
X77395 Genomic DNA Translation: CAA54574.1
Z71620 Genomic DNA Translation: CAA96282.1
BK006947 Genomic DNA Translation: DAA10547.1
PIRiS45129
RefSeqiNP_014403.3, NM_001183183.3

Genome annotation databases

EnsemblFungiiYNR006W; YNR006W; YNR006W
GeneIDi855739
KEGGisce:YNR006W

Similar proteinsi

Entry informationi

Entry nameiVPS27_YEAST
AccessioniPrimary (citable) accession number: P40343
Secondary accession number(s): D6W1I1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 5, 2010
Last modified: June 20, 2018
This is version 178 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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