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P40341 (YTA12_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 139. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial respiratory chain complexes assembly protein YTA12

EC=3.4.24.-
Alternative name(s):
Tat-binding homolog 12
Gene names
Name:YTA12
Synonyms:RCA1
Ordered Locus Names:YMR089C
ORF Names:YM9582.14C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length825 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as a component of the m-AAA protease complex which is a ATP-dependent metalloprotease mediating degradation of non-assembled mitochondrial inner membrane proteins. The complex is necessary for the assembly of mitochondrial respiratory chain and ATPase complexes. Function both in post-translational assembly and in the turnover of mistranslated or misfolded polypeptides. Ref.6 Ref.7

Cofactor

Binds 1 zinc ion per subunit Potential.

Subunit structure

Component of the 850 kDa m-AAA protease complex (YTA10-12) which consists of multiple copies of RCA1 AND AFG3. Ref.6

Subcellular location

Mitochondrion membrane; Multi-pass membrane protein Ref.6.

Miscellaneous

Present with 11500 molecules/cell in log phase SD medium.

Sequence similarities

In the N-terminal section; belongs to the AAA ATPase family.

In the C-terminal section; belongs to the peptidase M41 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

AFG3P399253EBI-14858,EBI-2317

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 825825Mitochondrial respiratory chain complexes assembly protein YTA12 HAMAP-Rule MF_01458
PRO_0000084676

Regions

Transmembrane178 – 19417Helical; Potential
Transmembrane294 – 31118Helical; Potential
Nucleotide binding388 – 3958ATP Potential

Sites

Active site6141 By similarity
Metal binding6131Zinc; catalytic By similarity
Metal binding6171Zinc; catalytic By similarity
Metal binding6891Zinc; catalytic By similarity

Experimental info

Mutagenesis6141E → Q: Abolishes proteolytic activity; impairs synthesis of respiratory chain proteins COB and COX1. No effect on m-AAA protease assembly. Ref.7
Sequence conflict1951D → G in AAT93118. Ref.5
Sequence conflict349 – 3502DV → EL in CAA56955. Ref.2
Sequence conflict6531I → V in AAA62606. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P40341 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 63CEBB9EF11B3DFC

FASTA82593,276
        10         20         30         40         50         60 
MLLLSWSRIA TKVVRRPVRF RSYYGLTHIK SLHTQYRLLN RLQENKSGNK NEDNNEDAKL 

        70         80         90        100        110        120 
NKEIPTDEEV EAIRKQVEKY IEQTKNNTIP ANWKEQKRKI DESIRRLEDA VLKQESNRIQ 

       130        140        150        160        170        180 
EERKEKEEEN GPSKAKSNRT KEQGYFEGNN SRNIPPPPPP PPPKPPLNDP SNPVSKNVNL 

       190        200        210        220        230        240 
FQIGLTFFLL SFLLDLLNSL EEQSEITWQD FREKLLAKGY VAKLIVVNKS MVKVMLNDNG 

       250        260        270        280        290        300 
KNQADNYGRN FYYFTIGSID SFEHKLQKAQ DELDIDKDFR IPVLYVQEGN WAKAMFQILP 

       310        320        330        340        350        360 
TVLMIAGIIW LTRRSAQAAG GSRGGIFGLS RSKAKKFNTE TDVKIKFKDV AGCDEAKEEI 

       370        380        390        400        410        420 
MEFVSFLKEP SRYEKMGAKI PRGAILSGPP GTGKTLLAKA TAGEAGVPFY FVSGSEFVEM 

       430        440        450        460        470        480 
FVGVGAARVR DLFKTARENA PSIVFIDEID AIGKARQKGN FSGANDEREN TLNQMLVEMD 

       490        500        510        520        530        540 
GFTPADHVVV LAGTNRPDIL DKALLRPGRF DRHINIDKPE LEGRKAIFAV HLHHLKLAGE 

       550        560        570        580        590        600 
IFDLKNRLAA LTPGFSGADI ANVCNEAALI AARSDEDAVK LNHFEQAIER VIGGVERKSK 

       610        620        630        640        650        660 
LLSPEEKKVV AYHEAGHAVC GWYLKYADPL LKVSIIPRGQ GALGYAQYLP GDIFLLTEQQ 

       670        680        690        700        710        720 
LKDRMTMSLG GRVSEELHFP SVTSGASDDF KKVTSMATAM VTELGMSDKI GWVNYQKRDD 

       730        740        750        760        770        780 
SDLTKPFSDE TGDIIDSEVY RIVQECHDRC TKLLKEKAED VEKIAQVLLK KEVLTREDMI 

       790        800        810        820 
DLLGKRPFPE RNDAFDKYLN DYETEKIRKE EEKNEKRNEP KPSTN 

« Hide

References

« Hide 'large scale' references
[1]"A new member of a family of ATPases is essential for assembly of mitochondrial respiratory chain and ATP synthetase complexes in Saccharomyces cerevisiae."
Tzagoloff A., Yue J., Jang J., Paul M.-F.
J. Biol. Chem. 269:26144-26151(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Identification of a set of yeast genes coding for a novel family of putative ATPases with high similarity to constituents of the 26S protease complex."
Schnall R., Mannhaupt G., Stucka R., Tauer R., Ehnle S., Schwarzlose C., Vetter I., Feldmann H.
Yeast 10:1141-1155(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"The YTA10-12 complex, an AAA protease with chaperone-like activity in the inner membrane of mitochondria."
Arlt H., Tauer R., Feldmann H., Neupert W., Langer T.
Cell 85:875-885(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE M-AAA PROTEASE COMPLEX, FUNCTION OF THE M-AAA PROTEASE COMPLEX, SUBCELLULAR LOCATION.
[7]"The formation of respiratory chain complexes in mitochondria is under the proteolytic control of the m-AAA protease."
Arlt H., Steglich G., Perryman R., Guiard B., Neupert W., Langer T.
EMBO J. 17:4837-4847(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF THE M-AAA PROTEASE COMPLEX, MUTAGENESIS OF GLU-614.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U09358 Genomic DNA. Translation: AAA62606.1.
X81068 Genomic DNA. Translation: CAA56955.1.
Z49259 Genomic DNA. Translation: CAA89236.1.
AY693099 Genomic DNA. Translation: AAT93118.1.
BK006946 Genomic DNA. Translation: DAA09986.1.
PIRS54465.
RefSeqNP_013807.1. NM_001182589.1.

3D structure databases

ProteinModelPortalP40341.
SMRP40341. Positions 205-290, 344-778.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35264. 122 interactions.
DIPDIP-889N.
IntActP40341. 9 interactions.
MINTMINT-596896.
STRING4932.YMR089C.

Protein family/group databases

MEROPSM41.003.

Proteomic databases

MaxQBP40341.
PaxDbP40341.
PeptideAtlasP40341.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR089C; YMR089C; YMR089C.
GeneID855114.
KEGGsce:YMR089C.

Organism-specific databases

CYGDYMR089c.
SGDS000004695. YTA12.

Phylogenomic databases

eggNOGCOG0465.
GeneTreeENSGT00730000110911.
KOK08956.
OMAVCGWYLK.
OrthoDBEOG73V6TS.

Enzyme and pathway databases

BioCycYEAST:G3O-32789-MONOMER.

Gene expression databases

GenevestigatorP40341.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
HAMAPMF_01458. FtsH.
InterProIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR005936. FtsH.
IPR027417. P-loop_NTPase.
IPR011546. Pept_M41_FtsH_extracell.
IPR000642. Peptidase_M41.
[Graphical view]
PfamPF00004. AAA. 1 hit.
PF06480. FtsH_ext. 1 hit.
PF01434. Peptidase_M41. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR01241. FtsH_fam. 1 hit.
PROSITEPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio978455.

Entry information

Entry nameYTA12_YEAST
AccessionPrimary (citable) accession number: P40341
Secondary accession number(s): D6VZR2, E9P917
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries