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P40341

- YTA12_YEAST

UniProt

P40341 - YTA12_YEAST

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Protein
Mitochondrial respiratory chain complexes assembly protein YTA12
Gene
YTA12, RCA1, YMR089C, YM9582.14C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Acts as a component of the m-AAA protease complex which is a ATP-dependent metalloprotease mediating degradation of non-assembled mitochondrial inner membrane proteins. The complex is necessary for the assembly of mitochondrial respiratory chain and ATPase complexes. Function both in post-translational assembly and in the turnover of mistranslated or misfolded polypeptides.2 Publications

Cofactori

Binds 1 zinc ion per subunit Reviewed prediction.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi613 – 6131Zinc; catalytic By similarity
Active sitei614 – 6141 By similarity
Metal bindingi617 – 6171Zinc; catalytic By similarity
Metal bindingi689 – 6891Zinc; catalytic By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi388 – 3958ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: SGD
  2. ATPase activity Source: SGD
  3. metalloendopeptidase activity Source: InterPro
  4. metallopeptidase activity Source: SGD
  5. protein binding Source: IntAct
  6. zinc ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. protein complex assembly Source: SGD
  3. protein import into mitochondrial intermembrane space Source: SGD
  4. proteolysis Source: SGD
  5. signal peptide processing Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32789-MONOMER.

Protein family/group databases

MEROPSiM41.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial respiratory chain complexes assembly protein YTA12 (EC:3.4.24.-)
Alternative name(s):
Tat-binding homolog 12
Gene namesi
Name:YTA12
Synonyms:RCA1
Ordered Locus Names:YMR089C
ORF Names:YM9582.14C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR089c.
SGDiS000004695. YTA12.

Subcellular locationi

Mitochondrion membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei178 – 19417Helical; Reviewed prediction
Add
BLAST
Transmembranei294 – 31118Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. m-AAA complex Source: SGD
  2. mitochondrial inner boundary membrane Source: SGD
  3. mitochondrial inner membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi614 – 6141E → Q: Abolishes proteolytic activity; impairs synthesis of respiratory chain proteins COB and COX1. No effect on m-AAA protease assembly. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 825825Mitochondrial respiratory chain complexes assembly protein YTA12UniRule annotation
PRO_0000084676Add
BLAST

Proteomic databases

MaxQBiP40341.
PaxDbiP40341.
PeptideAtlasiP40341.

Expressioni

Gene expression databases

GenevestigatoriP40341.

Interactioni

Subunit structurei

Component of the 850 kDa m-AAA protease complex (YTA10-12) which consists of multiple copies of RCA1 AND AFG3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
AFG3P399253EBI-14858,EBI-2317

Protein-protein interaction databases

BioGridi35264. 122 interactions.
DIPiDIP-889N.
IntActiP40341. 9 interactions.
MINTiMINT-596896.
STRINGi4932.YMR089C.

Structurei

3D structure databases

ProteinModelPortaliP40341.
SMRiP40341. Positions 205-290, 344-778.

Family & Domainsi

Sequence similaritiesi

In the N-terminal section; belongs to the AAA ATPase family.
In the C-terminal section; belongs to the peptidase M41 family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0465.
GeneTreeiENSGT00730000110911.
KOiK08956.
OMAiVCGWYLK.
OrthoDBiEOG73V6TS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_01458. FtsH.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR005936. FtsH.
IPR027417. P-loop_NTPase.
IPR011546. Pept_M41_FtsH_extracell.
IPR000642. Peptidase_M41.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF06480. FtsH_ext. 1 hit.
PF01434. Peptidase_M41. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01241. FtsH_fam. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40341-1 [UniParc]FASTAAdd to Basket

« Hide

MLLLSWSRIA TKVVRRPVRF RSYYGLTHIK SLHTQYRLLN RLQENKSGNK    50
NEDNNEDAKL NKEIPTDEEV EAIRKQVEKY IEQTKNNTIP ANWKEQKRKI 100
DESIRRLEDA VLKQESNRIQ EERKEKEEEN GPSKAKSNRT KEQGYFEGNN 150
SRNIPPPPPP PPPKPPLNDP SNPVSKNVNL FQIGLTFFLL SFLLDLLNSL 200
EEQSEITWQD FREKLLAKGY VAKLIVVNKS MVKVMLNDNG KNQADNYGRN 250
FYYFTIGSID SFEHKLQKAQ DELDIDKDFR IPVLYVQEGN WAKAMFQILP 300
TVLMIAGIIW LTRRSAQAAG GSRGGIFGLS RSKAKKFNTE TDVKIKFKDV 350
AGCDEAKEEI MEFVSFLKEP SRYEKMGAKI PRGAILSGPP GTGKTLLAKA 400
TAGEAGVPFY FVSGSEFVEM FVGVGAARVR DLFKTARENA PSIVFIDEID 450
AIGKARQKGN FSGANDEREN TLNQMLVEMD GFTPADHVVV LAGTNRPDIL 500
DKALLRPGRF DRHINIDKPE LEGRKAIFAV HLHHLKLAGE IFDLKNRLAA 550
LTPGFSGADI ANVCNEAALI AARSDEDAVK LNHFEQAIER VIGGVERKSK 600
LLSPEEKKVV AYHEAGHAVC GWYLKYADPL LKVSIIPRGQ GALGYAQYLP 650
GDIFLLTEQQ LKDRMTMSLG GRVSEELHFP SVTSGASDDF KKVTSMATAM 700
VTELGMSDKI GWVNYQKRDD SDLTKPFSDE TGDIIDSEVY RIVQECHDRC 750
TKLLKEKAED VEKIAQVLLK KEVLTREDMI DLLGKRPFPE RNDAFDKYLN 800
DYETEKIRKE EEKNEKRNEP KPSTN 825
Length:825
Mass (Da):93,276
Last modified:October 1, 1996 - v2
Checksum:i63CEBB9EF11B3DFC
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti195 – 1951D → G in AAT93118. 1 Publication
Sequence conflicti349 – 3502DV → EL in CAA56955. 1 Publication
Sequence conflicti653 – 6531I → V in AAA62606. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U09358 Genomic DNA. Translation: AAA62606.1.
X81068 Genomic DNA. Translation: CAA56955.1.
Z49259 Genomic DNA. Translation: CAA89236.1.
AY693099 Genomic DNA. Translation: AAT93118.1.
BK006946 Genomic DNA. Translation: DAA09986.1.
PIRiS54465.
RefSeqiNP_013807.1. NM_001182589.1.

Genome annotation databases

EnsemblFungiiYMR089C; YMR089C; YMR089C.
GeneIDi855114.
KEGGisce:YMR089C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U09358 Genomic DNA. Translation: AAA62606.1 .
X81068 Genomic DNA. Translation: CAA56955.1 .
Z49259 Genomic DNA. Translation: CAA89236.1 .
AY693099 Genomic DNA. Translation: AAT93118.1 .
BK006946 Genomic DNA. Translation: DAA09986.1 .
PIRi S54465.
RefSeqi NP_013807.1. NM_001182589.1.

3D structure databases

ProteinModelPortali P40341.
SMRi P40341. Positions 205-290, 344-778.
ModBasei Search...

Protein-protein interaction databases

BioGridi 35264. 122 interactions.
DIPi DIP-889N.
IntActi P40341. 9 interactions.
MINTi MINT-596896.
STRINGi 4932.YMR089C.

Protein family/group databases

MEROPSi M41.003.

Proteomic databases

MaxQBi P40341.
PaxDbi P40341.
PeptideAtlasi P40341.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR089C ; YMR089C ; YMR089C .
GeneIDi 855114.
KEGGi sce:YMR089C.

Organism-specific databases

CYGDi YMR089c.
SGDi S000004695. YTA12.

Phylogenomic databases

eggNOGi COG0465.
GeneTreei ENSGT00730000110911.
KOi K08956.
OMAi VCGWYLK.
OrthoDBi EOG73V6TS.

Enzyme and pathway databases

BioCyci YEAST:G3O-32789-MONOMER.

Miscellaneous databases

NextBioi 978455.

Gene expression databases

Genevestigatori P40341.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
HAMAPi MF_01458. FtsH.
InterProi IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR005936. FtsH.
IPR027417. P-loop_NTPase.
IPR011546. Pept_M41_FtsH_extracell.
IPR000642. Peptidase_M41.
[Graphical view ]
Pfami PF00004. AAA. 1 hit.
PF06480. FtsH_ext. 1 hit.
PF01434. Peptidase_M41. 1 hit.
[Graphical view ]
SMARTi SM00382. AAA. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR01241. FtsH_fam. 1 hit.
PROSITEi PS00674. AAA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A new member of a family of ATPases is essential for assembly of mitochondrial respiratory chain and ATP synthetase complexes in Saccharomyces cerevisiae."
    Tzagoloff A., Yue J., Jang J., Paul M.-F.
    J. Biol. Chem. 269:26144-26151(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Identification of a set of yeast genes coding for a novel family of putative ATPases with high similarity to constituents of the 26S protease complex."
    Schnall R., Mannhaupt G., Stucka R., Tauer R., Ehnle S., Schwarzlose C., Vetter I., Feldmann H.
    Yeast 10:1141-1155(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "The YTA10-12 complex, an AAA protease with chaperone-like activity in the inner membrane of mitochondria."
    Arlt H., Tauer R., Feldmann H., Neupert W., Langer T.
    Cell 85:875-885(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE M-AAA PROTEASE COMPLEX, FUNCTION OF THE M-AAA PROTEASE COMPLEX, SUBCELLULAR LOCATION.
  7. "The formation of respiratory chain complexes in mitochondria is under the proteolytic control of the m-AAA protease."
    Arlt H., Steglich G., Perryman R., Guiard B., Neupert W., Langer T.
    EMBO J. 17:4837-4847(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE M-AAA PROTEASE COMPLEX, MUTAGENESIS OF GLU-614.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiYTA12_YEAST
AccessioniPrimary (citable) accession number: P40341
Secondary accession number(s): D6VZR2, E9P917
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 11500 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

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