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Protein

Mitochondrial respiratory chain complexes assembly protein YTA12

Gene

YTA12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a component of the m-AAA protease complex which is a ATP-dependent metalloprotease mediating degradation of non-assembled mitochondrial inner membrane proteins. The complex is necessary for the assembly of mitochondrial respiratory chain and ATPase complexes. Function both in post-translational assembly and in the turnover of mistranslated or misfolded polypeptides.2 Publications

Cofactori

Zn2+CuratedNote: Binds 1 zinc ion per subunit.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi613 – 6131Zinc; catalyticBy similarity
Active sitei614 – 6141By similarity
Metal bindingi617 – 6171Zinc; catalyticBy similarity
Metal bindingi689 – 6891Zinc; catalyticBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi388 – 3958ATPSequence Analysis

GO - Molecular functioni

  • ATPase activity Source: SGD
  • ATP binding Source: SGD
  • metalloendopeptidase activity Source: InterPro
  • metallopeptidase activity Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • protein complex assembly Source: SGD
  • protein import into mitochondrial intermembrane space Source: SGD
  • proteolysis Source: SGD
  • signal peptide processing Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32789-MONOMER.
BRENDAi3.4.24.B18. 984.

Protein family/group databases

MEROPSiM41.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial respiratory chain complexes assembly protein YTA12 (EC:3.4.24.-)
Alternative name(s):
Tat-binding homolog 12
Gene namesi
Name:YTA12
Synonyms:RCA1
Ordered Locus Names:YMR089C
ORF Names:YM9582.14C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIII

Organism-specific databases

CYGDiYMR089c.
EuPathDBiFungiDB:YMR089C.
SGDiS000004695. YTA12.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei178 – 19417HelicalSequence AnalysisAdd
BLAST
Transmembranei294 – 31118HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • m-AAA complex Source: SGD
  • mitochondrial inner boundary membrane Source: SGD
  • mitochondrial inner membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi614 – 6141E → Q: Abolishes proteolytic activity; impairs synthesis of respiratory chain proteins COB and COX1. No effect on m-AAA protease assembly. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 825825Mitochondrial respiratory chain complexes assembly protein YTA12PRO_0000084676Add
BLAST

Proteomic databases

MaxQBiP40341.
PaxDbiP40341.
PeptideAtlasiP40341.

Interactioni

Subunit structurei

Component of the 850 kDa m-AAA protease complex (YTA10-12) which consists of multiple copies of RCA1 AND AFG3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
AFG3P399253EBI-14858,EBI-2317

Protein-protein interaction databases

BioGridi35264. 122 interactions.
DIPiDIP-889N.
IntActiP40341. 9 interactions.
MINTiMINT-596896.

Structurei

3D structure databases

ProteinModelPortaliP40341.
SMRiP40341. Positions 205-290, 344-778.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

In the N-terminal section; belongs to the AAA ATPase family.Curated
In the C-terminal section; belongs to the peptidase M41 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0465.
GeneTreeiENSGT00800000124192.
InParanoidiP40341.
KOiK08956.
OMAiVCGWYLK.
OrthoDBiEOG73V6TS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_01458. FtsH.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR005936. FtsH.
IPR027417. P-loop_NTPase.
IPR011546. Pept_M41_FtsH_extracell.
IPR000642. Peptidase_M41.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF06480. FtsH_ext. 1 hit.
PF01434. Peptidase_M41. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01241. FtsH_fam. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40341-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLLSWSRIA TKVVRRPVRF RSYYGLTHIK SLHTQYRLLN RLQENKSGNK
60 70 80 90 100
NEDNNEDAKL NKEIPTDEEV EAIRKQVEKY IEQTKNNTIP ANWKEQKRKI
110 120 130 140 150
DESIRRLEDA VLKQESNRIQ EERKEKEEEN GPSKAKSNRT KEQGYFEGNN
160 170 180 190 200
SRNIPPPPPP PPPKPPLNDP SNPVSKNVNL FQIGLTFFLL SFLLDLLNSL
210 220 230 240 250
EEQSEITWQD FREKLLAKGY VAKLIVVNKS MVKVMLNDNG KNQADNYGRN
260 270 280 290 300
FYYFTIGSID SFEHKLQKAQ DELDIDKDFR IPVLYVQEGN WAKAMFQILP
310 320 330 340 350
TVLMIAGIIW LTRRSAQAAG GSRGGIFGLS RSKAKKFNTE TDVKIKFKDV
360 370 380 390 400
AGCDEAKEEI MEFVSFLKEP SRYEKMGAKI PRGAILSGPP GTGKTLLAKA
410 420 430 440 450
TAGEAGVPFY FVSGSEFVEM FVGVGAARVR DLFKTARENA PSIVFIDEID
460 470 480 490 500
AIGKARQKGN FSGANDEREN TLNQMLVEMD GFTPADHVVV LAGTNRPDIL
510 520 530 540 550
DKALLRPGRF DRHINIDKPE LEGRKAIFAV HLHHLKLAGE IFDLKNRLAA
560 570 580 590 600
LTPGFSGADI ANVCNEAALI AARSDEDAVK LNHFEQAIER VIGGVERKSK
610 620 630 640 650
LLSPEEKKVV AYHEAGHAVC GWYLKYADPL LKVSIIPRGQ GALGYAQYLP
660 670 680 690 700
GDIFLLTEQQ LKDRMTMSLG GRVSEELHFP SVTSGASDDF KKVTSMATAM
710 720 730 740 750
VTELGMSDKI GWVNYQKRDD SDLTKPFSDE TGDIIDSEVY RIVQECHDRC
760 770 780 790 800
TKLLKEKAED VEKIAQVLLK KEVLTREDMI DLLGKRPFPE RNDAFDKYLN
810 820
DYETEKIRKE EEKNEKRNEP KPSTN
Length:825
Mass (Da):93,276
Last modified:October 1, 1996 - v2
Checksum:i63CEBB9EF11B3DFC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti195 – 1951D → G in AAT93118 (PubMed:17322287).Curated
Sequence conflicti349 – 3502DV → EL in CAA56955 (PubMed:7754704).Curated
Sequence conflicti653 – 6531I → V in AAA62606 (PubMed:7929327).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09358 Genomic DNA. Translation: AAA62606.1.
X81068 Genomic DNA. Translation: CAA56955.1.
Z49259 Genomic DNA. Translation: CAA89236.1.
AY693099 Genomic DNA. Translation: AAT93118.1.
BK006946 Genomic DNA. Translation: DAA09986.1.
PIRiS54465.
RefSeqiNP_013807.1. NM_001182589.1.

Genome annotation databases

EnsemblFungiiYMR089C; YMR089C; YMR089C.
GeneIDi855114.
KEGGisce:YMR089C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09358 Genomic DNA. Translation: AAA62606.1.
X81068 Genomic DNA. Translation: CAA56955.1.
Z49259 Genomic DNA. Translation: CAA89236.1.
AY693099 Genomic DNA. Translation: AAT93118.1.
BK006946 Genomic DNA. Translation: DAA09986.1.
PIRiS54465.
RefSeqiNP_013807.1. NM_001182589.1.

3D structure databases

ProteinModelPortaliP40341.
SMRiP40341. Positions 205-290, 344-778.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35264. 122 interactions.
DIPiDIP-889N.
IntActiP40341. 9 interactions.
MINTiMINT-596896.

Protein family/group databases

MEROPSiM41.003.

Proteomic databases

MaxQBiP40341.
PaxDbiP40341.
PeptideAtlasiP40341.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR089C; YMR089C; YMR089C.
GeneIDi855114.
KEGGisce:YMR089C.

Organism-specific databases

CYGDiYMR089c.
EuPathDBiFungiDB:YMR089C.
SGDiS000004695. YTA12.

Phylogenomic databases

eggNOGiCOG0465.
GeneTreeiENSGT00800000124192.
InParanoidiP40341.
KOiK08956.
OMAiVCGWYLK.
OrthoDBiEOG73V6TS.

Enzyme and pathway databases

BioCyciYEAST:G3O-32789-MONOMER.
BRENDAi3.4.24.B18. 984.

Miscellaneous databases

NextBioi978455.
PROiP40341.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_01458. FtsH.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR005936. FtsH.
IPR027417. P-loop_NTPase.
IPR011546. Pept_M41_FtsH_extracell.
IPR000642. Peptidase_M41.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF06480. FtsH_ext. 1 hit.
PF01434. Peptidase_M41. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01241. FtsH_fam. 1 hit.
PROSITEiPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A new member of a family of ATPases is essential for assembly of mitochondrial respiratory chain and ATP synthetase complexes in Saccharomyces cerevisiae."
    Tzagoloff A., Yue J., Jang J., Paul M.-F.
    J. Biol. Chem. 269:26144-26151(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Identification of a set of yeast genes coding for a novel family of putative ATPases with high similarity to constituents of the 26S protease complex."
    Schnall R., Mannhaupt G., Stucka R., Tauer R., Ehnle S., Schwarzlose C., Vetter I., Feldmann H.
    Yeast 10:1141-1155(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "The YTA10-12 complex, an AAA protease with chaperone-like activity in the inner membrane of mitochondria."
    Arlt H., Tauer R., Feldmann H., Neupert W., Langer T.
    Cell 85:875-885(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE M-AAA PROTEASE COMPLEX, FUNCTION OF THE M-AAA PROTEASE COMPLEX, SUBCELLULAR LOCATION.
  7. "The formation of respiratory chain complexes in mitochondria is under the proteolytic control of the m-AAA protease."
    Arlt H., Steglich G., Perryman R., Guiard B., Neupert W., Langer T.
    EMBO J. 17:4837-4847(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION OF THE M-AAA PROTEASE COMPLEX, MUTAGENESIS OF GLU-614.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiYTA12_YEAST
AccessioniPrimary (citable) accession number: P40341
Secondary accession number(s): D6VZR2, E9P917
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: July 22, 2015
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 11500 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.