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P40341

- YTA12_YEAST

UniProt

P40341 - YTA12_YEAST

Protein

Mitochondrial respiratory chain complexes assembly protein YTA12

Gene

YTA12

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 2 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Acts as a component of the m-AAA protease complex which is a ATP-dependent metalloprotease mediating degradation of non-assembled mitochondrial inner membrane proteins. The complex is necessary for the assembly of mitochondrial respiratory chain and ATPase complexes. Function both in post-translational assembly and in the turnover of mistranslated or misfolded polypeptides.2 Publications

    Cofactori

    Binds 1 zinc ion per subunit.Curated

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi613 – 6131Zinc; catalyticBy similarity
    Active sitei614 – 6141By similarity
    Metal bindingi617 – 6171Zinc; catalyticBy similarity
    Metal bindingi689 – 6891Zinc; catalyticBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi388 – 3958ATPSequence Analysis

    GO - Molecular functioni

    1. ATPase activity Source: SGD
    2. ATP binding Source: SGD
    3. metalloendopeptidase activity Source: InterPro
    4. metallopeptidase activity Source: SGD
    5. protein binding Source: IntAct
    6. zinc ion binding Source: InterPro

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. protein complex assembly Source: SGD
    3. protein import into mitochondrial intermembrane space Source: SGD
    4. proteolysis Source: SGD
    5. signal peptide processing Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32789-MONOMER.

    Protein family/group databases

    MEROPSiM41.003.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial respiratory chain complexes assembly protein YTA12 (EC:3.4.24.-)
    Alternative name(s):
    Tat-binding homolog 12
    Gene namesi
    Name:YTA12
    Synonyms:RCA1
    Ordered Locus Names:YMR089C
    ORF Names:YM9582.14C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR089c.
    SGDiS000004695. YTA12.

    Subcellular locationi

    Mitochondrion membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. m-AAA complex Source: SGD
    2. mitochondrial inner boundary membrane Source: SGD
    3. mitochondrial inner membrane Source: SGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi614 – 6141E → Q: Abolishes proteolytic activity; impairs synthesis of respiratory chain proteins COB and COX1. No effect on m-AAA protease assembly. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 825825Mitochondrial respiratory chain complexes assembly protein YTA12PRO_0000084676Add
    BLAST

    Proteomic databases

    MaxQBiP40341.
    PaxDbiP40341.
    PeptideAtlasiP40341.

    Expressioni

    Gene expression databases

    GenevestigatoriP40341.

    Interactioni

    Subunit structurei

    Component of the 850 kDa m-AAA protease complex (YTA10-12) which consists of multiple copies of RCA1 AND AFG3.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    AFG3P399253EBI-14858,EBI-2317

    Protein-protein interaction databases

    BioGridi35264. 122 interactions.
    DIPiDIP-889N.
    IntActiP40341. 9 interactions.
    MINTiMINT-596896.
    STRINGi4932.YMR089C.

    Structurei

    3D structure databases

    ProteinModelPortaliP40341.
    SMRiP40341. Positions 205-290, 344-778.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei178 – 19417HelicalSequence AnalysisAdd
    BLAST
    Transmembranei294 – 31118HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    In the N-terminal section; belongs to the AAA ATPase family.Curated
    In the C-terminal section; belongs to the peptidase M41 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0465.
    GeneTreeiENSGT00730000110911.
    KOiK08956.
    OMAiVCGWYLK.
    OrthoDBiEOG73V6TS.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    HAMAPiMF_01458. FtsH.
    InterProiIPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR005936. FtsH.
    IPR027417. P-loop_NTPase.
    IPR011546. Pept_M41_FtsH_extracell.
    IPR000642. Peptidase_M41.
    [Graphical view]
    PfamiPF00004. AAA. 1 hit.
    PF06480. FtsH_ext. 1 hit.
    PF01434. Peptidase_M41. 1 hit.
    [Graphical view]
    SMARTiSM00382. AAA. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR01241. FtsH_fam. 1 hit.
    PROSITEiPS00674. AAA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40341-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLLLSWSRIA TKVVRRPVRF RSYYGLTHIK SLHTQYRLLN RLQENKSGNK    50
    NEDNNEDAKL NKEIPTDEEV EAIRKQVEKY IEQTKNNTIP ANWKEQKRKI 100
    DESIRRLEDA VLKQESNRIQ EERKEKEEEN GPSKAKSNRT KEQGYFEGNN 150
    SRNIPPPPPP PPPKPPLNDP SNPVSKNVNL FQIGLTFFLL SFLLDLLNSL 200
    EEQSEITWQD FREKLLAKGY VAKLIVVNKS MVKVMLNDNG KNQADNYGRN 250
    FYYFTIGSID SFEHKLQKAQ DELDIDKDFR IPVLYVQEGN WAKAMFQILP 300
    TVLMIAGIIW LTRRSAQAAG GSRGGIFGLS RSKAKKFNTE TDVKIKFKDV 350
    AGCDEAKEEI MEFVSFLKEP SRYEKMGAKI PRGAILSGPP GTGKTLLAKA 400
    TAGEAGVPFY FVSGSEFVEM FVGVGAARVR DLFKTARENA PSIVFIDEID 450
    AIGKARQKGN FSGANDEREN TLNQMLVEMD GFTPADHVVV LAGTNRPDIL 500
    DKALLRPGRF DRHINIDKPE LEGRKAIFAV HLHHLKLAGE IFDLKNRLAA 550
    LTPGFSGADI ANVCNEAALI AARSDEDAVK LNHFEQAIER VIGGVERKSK 600
    LLSPEEKKVV AYHEAGHAVC GWYLKYADPL LKVSIIPRGQ GALGYAQYLP 650
    GDIFLLTEQQ LKDRMTMSLG GRVSEELHFP SVTSGASDDF KKVTSMATAM 700
    VTELGMSDKI GWVNYQKRDD SDLTKPFSDE TGDIIDSEVY RIVQECHDRC 750
    TKLLKEKAED VEKIAQVLLK KEVLTREDMI DLLGKRPFPE RNDAFDKYLN 800
    DYETEKIRKE EEKNEKRNEP KPSTN 825
    Length:825
    Mass (Da):93,276
    Last modified:October 1, 1996 - v2
    Checksum:i63CEBB9EF11B3DFC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti195 – 1951D → G in AAT93118. (PubMed:17322287)Curated
    Sequence conflicti349 – 3502DV → EL in CAA56955. (PubMed:7754704)Curated
    Sequence conflicti653 – 6531I → V in AAA62606. (PubMed:7929327)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09358 Genomic DNA. Translation: AAA62606.1.
    X81068 Genomic DNA. Translation: CAA56955.1.
    Z49259 Genomic DNA. Translation: CAA89236.1.
    AY693099 Genomic DNA. Translation: AAT93118.1.
    BK006946 Genomic DNA. Translation: DAA09986.1.
    PIRiS54465.
    RefSeqiNP_013807.1. NM_001182589.1.

    Genome annotation databases

    EnsemblFungiiYMR089C; YMR089C; YMR089C.
    GeneIDi855114.
    KEGGisce:YMR089C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09358 Genomic DNA. Translation: AAA62606.1 .
    X81068 Genomic DNA. Translation: CAA56955.1 .
    Z49259 Genomic DNA. Translation: CAA89236.1 .
    AY693099 Genomic DNA. Translation: AAT93118.1 .
    BK006946 Genomic DNA. Translation: DAA09986.1 .
    PIRi S54465.
    RefSeqi NP_013807.1. NM_001182589.1.

    3D structure databases

    ProteinModelPortali P40341.
    SMRi P40341. Positions 205-290, 344-778.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35264. 122 interactions.
    DIPi DIP-889N.
    IntActi P40341. 9 interactions.
    MINTi MINT-596896.
    STRINGi 4932.YMR089C.

    Protein family/group databases

    MEROPSi M41.003.

    Proteomic databases

    MaxQBi P40341.
    PaxDbi P40341.
    PeptideAtlasi P40341.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR089C ; YMR089C ; YMR089C .
    GeneIDi 855114.
    KEGGi sce:YMR089C.

    Organism-specific databases

    CYGDi YMR089c.
    SGDi S000004695. YTA12.

    Phylogenomic databases

    eggNOGi COG0465.
    GeneTreei ENSGT00730000110911.
    KOi K08956.
    OMAi VCGWYLK.
    OrthoDBi EOG73V6TS.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32789-MONOMER.

    Miscellaneous databases

    NextBioi 978455.

    Gene expression databases

    Genevestigatori P40341.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    HAMAPi MF_01458. FtsH.
    InterProi IPR003593. AAA+_ATPase.
    IPR003959. ATPase_AAA_core.
    IPR003960. ATPase_AAA_CS.
    IPR005936. FtsH.
    IPR027417. P-loop_NTPase.
    IPR011546. Pept_M41_FtsH_extracell.
    IPR000642. Peptidase_M41.
    [Graphical view ]
    Pfami PF00004. AAA. 1 hit.
    PF06480. FtsH_ext. 1 hit.
    PF01434. Peptidase_M41. 1 hit.
    [Graphical view ]
    SMARTi SM00382. AAA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR01241. FtsH_fam. 1 hit.
    PROSITEi PS00674. AAA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A new member of a family of ATPases is essential for assembly of mitochondrial respiratory chain and ATP synthetase complexes in Saccharomyces cerevisiae."
      Tzagoloff A., Yue J., Jang J., Paul M.-F.
      J. Biol. Chem. 269:26144-26151(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Identification of a set of yeast genes coding for a novel family of putative ATPases with high similarity to constituents of the 26S protease complex."
      Schnall R., Mannhaupt G., Stucka R., Tauer R., Ehnle S., Schwarzlose C., Vetter I., Feldmann H.
      Yeast 10:1141-1155(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. "The YTA10-12 complex, an AAA protease with chaperone-like activity in the inner membrane of mitochondria."
      Arlt H., Tauer R., Feldmann H., Neupert W., Langer T.
      Cell 85:875-885(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE M-AAA PROTEASE COMPLEX, FUNCTION OF THE M-AAA PROTEASE COMPLEX, SUBCELLULAR LOCATION.
    7. "The formation of respiratory chain complexes in mitochondria is under the proteolytic control of the m-AAA protease."
      Arlt H., Steglich G., Perryman R., Guiard B., Neupert W., Langer T.
      EMBO J. 17:4837-4847(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION OF THE M-AAA PROTEASE COMPLEX, MUTAGENESIS OF GLU-614.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiYTA12_YEAST
    AccessioniPrimary (citable) accession number: P40341
    Secondary accession number(s): D6VZR2, E9P917
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 140 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 11500 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3