Mitochondrial respiratory chain complexes assembly protein YTA12 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P40341 (YTA12_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 129. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Mitochondrial respiratory chain complexes assembly protein YTA12
EC=3.4.24.-

Alternative name(s):
Tat-binding homolog 12

Gene names

Name:	YTA12
Synonyms:	RCA1
Ordered Locus Names:	YMR089C
ORF Names:	YM9582.14C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	825 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a component of the m-AAA protease complex which is a ATP-dependent metalloprotease mediating degradation of non-assembled mitochondrial inner membrane proteins. The complex is necessary for the assembly of mitochondrial respiratory chain and ATPase complexes. Function both in post-translational assembly and in the turnover of mistranslated or misfolded polypeptides. Ref.6 Ref.7
Cofactor	Binds 1 zinc ion per subunit Potential.
Subunit structure	Component of the 850 kDa m-AAA protease complex (YTA10-12) which consists of multiple copies of RCA1 AND AFG3. Ref.6
Subcellular location	Mitochondrion membrane; Multi-pass membrane protein Ref.6.
Miscellaneous	Present with 11500 molecules/cell in log phase SD medium.
Sequence similarities	In the N-terminal section; belongs to the AAA ATPase family. In the C-terminal section; belongs to the peptidase M41 family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein catabolic process Inferred from electronic annotation. Source: InterPro protein complex assembly Inferred from mutant phenotype Ref.6. Source: SGD protein import into mitochondrial intermembrane space Traceable author statement PubMed 12417197. Source: SGD proteolysis Inferred from mutant phenotype Ref.6. Source: SGD signal peptide processing Inferred from mutant phenotype PubMed 12417197. Source: SGD
Cellular_component	m-AAA complex Inferred from direct assay Ref.6. Source: SGD mitochondrial inner boundary membrane Inferred from direct assay PubMed 19019989. Source: SGD
Molecular_function	ATP binding Inferred from direct assay PubMed 19748354. Source: SGD ATPase activity Inferred from direct assay PubMed 19748354. Source: SGD metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro metallopeptidase activity Inferred from mutant phenotype Ref.6. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AFG3	P39925	3	EBI-14858,EBI-2317

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 825

825

Mitochondrial respiratory chain complexes assembly protein YTA12

PRO_0000084676

Regions

Transmembrane

178 – 194

Helical; Potential

Transmembrane

294 – 311

Helical; Potential

Nucleotide binding

388 – 395

ATP Potential

Sites

Active site

614

By similarity

Metal binding

613

Zinc; catalytic By similarity

Metal binding

617

Zinc; catalytic By similarity

Metal binding

689

Zinc; catalytic By similarity

Experimental info

Mutagenesis

614

E → Q: Abolishes proteolytic activity; impairs synthesis of respiratory chain proteins COB and COX1. No effect on m-AAA protease assembly. Ref.7

Sequence conflict

195

D → G in AAT93118. Ref.5

Sequence conflict

349 – 350

DV → EL in CAA56955. Ref.2

Sequence conflict

653

I → V in AAA62606. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P40341 [UniParc].

Last modified October 1, 1996. Version 2.
Checksum: 63CEBB9EF11B3DFC
Show »

FASTA

825

93,276

        10         20         30         40         50         60 
MLLLSWSRIA TKVVRRPVRF RSYYGLTHIK SLHTQYRLLN RLQENKSGNK NEDNNEDAKL 

        70         80         90        100        110        120 
NKEIPTDEEV EAIRKQVEKY IEQTKNNTIP ANWKEQKRKI DESIRRLEDA VLKQESNRIQ 

       130        140        150        160        170        180 
EERKEKEEEN GPSKAKSNRT KEQGYFEGNN SRNIPPPPPP PPPKPPLNDP SNPVSKNVNL 

       190        200        210        220        230        240 
FQIGLTFFLL SFLLDLLNSL EEQSEITWQD FREKLLAKGY VAKLIVVNKS MVKVMLNDNG 

       250        260        270        280        290        300 
KNQADNYGRN FYYFTIGSID SFEHKLQKAQ DELDIDKDFR IPVLYVQEGN WAKAMFQILP 

       310        320        330        340        350        360 
TVLMIAGIIW LTRRSAQAAG GSRGGIFGLS RSKAKKFNTE TDVKIKFKDV AGCDEAKEEI 

       370        380        390        400        410        420 
MEFVSFLKEP SRYEKMGAKI PRGAILSGPP GTGKTLLAKA TAGEAGVPFY FVSGSEFVEM 

       430        440        450        460        470        480 
FVGVGAARVR DLFKTARENA PSIVFIDEID AIGKARQKGN FSGANDEREN TLNQMLVEMD 

       490        500        510        520        530        540 
GFTPADHVVV LAGTNRPDIL DKALLRPGRF DRHINIDKPE LEGRKAIFAV HLHHLKLAGE 

       550        560        570        580        590        600 
IFDLKNRLAA LTPGFSGADI ANVCNEAALI AARSDEDAVK LNHFEQAIER VIGGVERKSK 

       610        620        630        640        650        660 
LLSPEEKKVV AYHEAGHAVC GWYLKYADPL LKVSIIPRGQ GALGYAQYLP GDIFLLTEQQ 

       670        680        690        700        710        720 
LKDRMTMSLG GRVSEELHFP SVTSGASDDF KKVTSMATAM VTELGMSDKI GWVNYQKRDD 

       730        740        750        760        770        780 
SDLTKPFSDE TGDIIDSEVY RIVQECHDRC TKLLKEKAED VEKIAQVLLK KEVLTREDMI 

       790        800        810        820 
DLLGKRPFPE RNDAFDKYLN DYETEKIRKE EEKNEKRNEP KPSTN

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A new member of a family of ATPases is essential for assembly of mitochondrial respiratory chain and ATP synthetase complexes in Saccharomyces cerevisiae." Tzagoloff A., Yue J., Jang J., Paul M.-F. J. Biol. Chem. 269:26144-26151(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Identification of a set of yeast genes coding for a novel family of putative ATPases with high similarity to constituents of the 26S protease complex." Schnall R., Mannhaupt G., Stucka R., Tauer R., Ehnle S., Schwarzlose C., Vetter I., Feldmann H. Yeast 10:1141-1155(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"The YTA10-12 complex, an AAA protease with chaperone-like activity in the inner membrane of mitochondria." Arlt H., Tauer R., Feldmann H., Neupert W., Langer T. Cell 85:875-885(1996) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE M-AAA PROTEASE COMPLEX, FUNCTION OF THE M-AAA PROTEASE COMPLEX, SUBCELLULAR LOCATION.
[7]	"The formation of respiratory chain complexes in mitochondria is under the proteolytic control of the m-AAA protease." Arlt H., Steglich G., Perryman R., Guiard B., Neupert W., Langer T. EMBO J. 17:4837-4847(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION OF THE M-AAA PROTEASE COMPLEX, MUTAGENESIS OF GLU-614.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U09358 Genomic DNA. Translation: AAA62606.1. X81068 Genomic DNA. Translation: CAA56955.1. Z49259 Genomic DNA. Translation: CAA89236.1. AY693099 Genomic DNA. Translation: AAT93118.1. BK006946 Genomic DNA. Translation: DAA09986.1.
PIR	S54465.
RefSeq	NP_013807.1. NM_001182589.1.
3D structure databases
ProteinModelPortal	P40341.
SMR	P40341. Positions 205-290, 344-778.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-889N.
IntAct	P40341. 8 interactions.
MINT	MINT-596896.
STRING	4932.YMR089C.
Protein family/group databases
MEROPS	M41.003.
Proteomic databases
PaxDb	P40341.
PeptideAtlas	P40341.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR089C; YMR089C; YMR089C.
GeneID	855114.
KEGG	sce:YMR089C.
Organism-specific databases
CYGD	YMR089c.
SGD	S000004695. YTA12.
Phylogenomic databases
eggNOG	COG0465.
GeneTree	ENSGT00530000063070.
KO	K08956.
OMA	NFSGAND.
OrthoDB	EOG4J14HG.
Enzyme and pathway databases
BioCyc	YEAST:G3O-32789-MONOMER.
Gene expression databases
Genevestigator	P40341.
GermOnline	YMR089C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR005936. FtsH. IPR027417. P-loop_NTPase. IPR011546. Pept_M41_FtsH_extracell. IPR000642. Peptidase_M41. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF06480. FtsH_ext. 1 hit. PF01434. Peptidase_M41. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit.
TIGRFAMs	TIGR01241. FtsH_fam. 1 hit.
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978455.

Entry information

Entry name

YTA12_YEAST

Accession

Primary (citable) accession number: P40341
Secondary accession number(s): D6VZR2, E9P917

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 129 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents