Reviewed, UniProtKB/Swiss-Prot P40341 (RCA1_YEAST)

Names and origin

Protein names

Recommended name:
    Mitochondrial respiratory chain complexes assembly protein RCA1
    EC=3.4.24.-
Alternative name(s):
    TAT-binding homolog 12

Gene names

Name:	RCA1
Synonyms:	YTA12
Ordered Locus Names:	YMR089C
ORF Names:	YM9582.14C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	825 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts as a component of the m-AAA protease complex which is a ATP-dependent metalloprotease mediating degradation of non-assembled mitochondrial inner membrane proteins. The complex is necessary for the assembly of mitochondrial respiratory chain and ATPase complexes. Function both in post-translational assembly and in the turnover of mistranslated or misfolded polypeptides. Ref.4 Ref.5
Cofactor	Binds 1 zinc ion per subunit Potential.
Subunit structure	Component of the 850 kDa m-AAA protease complex (YTA10-12) which consists of multiple copies of RCA1 AND AFG3.
Subcellular location	Mitochondrion membrane; Multi-pass membrane protein.
Miscellaneous	Present with 11500 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	In the N-terminal section; belongs to the AAA ATPase family. In the C-terminal section; belongs to the peptidase M41 family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion
Domain	Transmembrane
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein complex assembly Ref.4 Inferred from mutant phenotype. Source: SGD protein import into mitochondrial intermembrane space Traceable author statement. Source: SGD proteolysis Ref.4 Inferred from mutant phenotype. Source: SGD signal peptide processing Inferred from mutant phenotype. Source: SGD
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell m-AAA complex Ref.4 Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
AFG3	P39925	1	EBI-14858,EBI-2317

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 825	825	Mitochondrial respiratory chain complexes assembly protein RCA1		PRO_0000084676
Regions
	Transmembrane	178 – 194	17	Potential
	Transmembrane	294 – 311	18	Potential
	Nucleotide binding	388 – 395	8	ATP Potential
Sites
	Active site	614	1	By similarity
	Metal binding	613	1	Zinc; catalytic By similarity
	Metal binding	617	1	Zinc; catalytic By similarity
Experimental info
	Mutagenesis	614	1	E → Q: Abolishes proteolytic activity; impairs synthesis of respiratory chain proteins COB and COX1. No effect on m-AAA protease assembly. Ref.5
	Sequence conflict	349 – 350	2	DV → EL in CAA56955. Ref.2
	Sequence conflict	653	1	I → V in AAA62606. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P40341-1 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 63CEBB9EF11B3DFC	FASTA	825	93,276
	10 20 30 40 50 60 MLLLSWSRIA TKVVRRPVRF RSYYGLTHIK SLHTQYRLLN RLQENKSGNK NEDNNEDAKL 70 80 90 100 110 120 NKEIPTDEEV EAIRKQVEKY IEQTKNNTIP ANWKEQKRKI DESIRRLEDA VLKQESNRIQ 130 140 150 160 170 180 EERKEKEEEN GPSKAKSNRT KEQGYFEGNN SRNIPPPPPP PPPKPPLNDP SNPVSKNVNL 190 200 210 220 230 240 FQIGLTFFLL SFLLDLLNSL EEQSEITWQD FREKLLAKGY VAKLIVVNKS MVKVMLNDNG 250 260 270 280 290 300 KNQADNYGRN FYYFTIGSID SFEHKLQKAQ DELDIDKDFR IPVLYVQEGN WAKAMFQILP 310 320 330 340 350 360 TVLMIAGIIW LTRRSAQAAG GSRGGIFGLS RSKAKKFNTE TDVKIKFKDV AGCDEAKEEI 370 380 390 400 410 420 MEFVSFLKEP SRYEKMGAKI PRGAILSGPP GTGKTLLAKA TAGEAGVPFY FVSGSEFVEM 430 440 450 460 470 480 FVGVGAARVR DLFKTARENA PSIVFIDEID AIGKARQKGN FSGANDEREN TLNQMLVEMD 490 500 510 520 530 540 GFTPADHVVV LAGTNRPDIL DKALLRPGRF DRHINIDKPE LEGRKAIFAV HLHHLKLAGE 550 560 570 580 590 600 IFDLKNRLAA LTPGFSGADI ANVCNEAALI AARSDEDAVK LNHFEQAIER VIGGVERKSK 610 620 630 640 650 660 LLSPEEKKVV AYHEAGHAVC GWYLKYADPL LKVSIIPRGQ GALGYAQYLP GDIFLLTEQQ 670 680 690 700 710 720 LKDRMTMSLG GRVSEELHFP SVTSGASDDF KKVTSMATAM VTELGMSDKI GWVNYQKRDD 730 740 750 760 770 780 SDLTKPFSDE TGDIIDSEVY RIVQECHDRC TKLLKEKAED VEKIAQVLLK KEVLTREDMI 790 800 810 820 DLLGKRPFPE RNDAFDKYLN DYETEKIRKE EEKNEKRNEP KPSTN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A new member of a family of ATPases is essential for assembly of mitochondrial respiratory chain and ATP synthetase complexes in Saccharomyces cerevisiae." Tzagoloff A., Yue J., Jang J., Paul M.-F. J. Biol. Chem. 269:26144-26151(1994) [PubMed: 7929327] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Identification of a set of yeast genes coding for a novel family of putative ATPases with high similarity to constituents of the 26S protease complex." Schnall R., Mannhaupt G., Stucka R., Tauer R., Ehnle S., Schwarzlose C., Vetter I., Feldmann H. Yeast 10:1141-1155(1994) [PubMed: 7754704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed: 9169872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	"The YTA10-12 complex, an AAA protease with chaperone-like activity in the inner membrane of mitochondria." Arlt H., Tauer R., Feldmann H., Neupert W., Langer T. Cell 85:875-885(1996) [PubMed: 8681382] [Abstract] Cited for: IDENTIFICATION IN THE M-AAA PROTEASE COMPLEX, FUNCTION OF THE M-AAA PROTEASE COMPLEX, SUBCELLULAR LOCATION.
[5]	"The formation of respiratory chain complexes in mitochondria is under the proteolytic control of the m-AAA protease." Arlt H., Steglich G., Perryman R., Guiard B., Neupert W., Langer T. EMBO J. 17:4837-4847(1998) [PubMed: 9707443] [Abstract] Cited for: FUNCTION OF THE M-AAA PROTEASE COMPLEX, MUTAGENESIS OF GLU-614.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U09358 Genomic DNA. Translation: AAA62606.1. X81068 Genomic DNA. Translation: CAA56955.1. Z49259 Genomic DNA. Translation: CAA89236.1.
PIR	S54465.
RefSeq	NP_013807.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:889N.
IntAct	P40341. 8 interactions.
STRING	P40341.
Protein family/group databases
MEROPS	M41.003.
Proteomic databases
PeptideAtlas	P40341.
Genome annotation databases
Ensembl	YMR089C; YMR089C; YMR089C; Saccharomyces cerevisiae. [Genome view]
GeneID	855114.
KEGG	sce:YMR089C.
NMPDR	fig|4932.3.peg.4854.
Organism-specific databases
CYGD	YMR089c.
SGD	S000004695. YTA12.
Phylogenomic databases
HOGENOM	P40341.
OMA	AVCGWYL
OrthoDB	EOG9Z6452
Gene expression databases
ArrayExpress	P40341.
Genevestigator	P40341.
GermOnline	YMR089C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR005936. Pept_M41_FtsH. IPR011546. Pept_M41_FtsH_extracell. IPR000642. Peptidase_M41. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF06480. FtsH_ext. 1 hit. PF01434. Peptidase_M41. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01241. FtsH_fam. 1 hit.
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	978455.

Entry information

Entry name

RCA1_YEAST

Accession

Primary (citable) accession number: P40341

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	October 1, 1996
Last modified:	November 24, 2009
This is version 94 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)