P40336 (VP26A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vacuolar protein sorting-associated protein 26A

Alternative name(s):
H<beta>58 protein
Short name=H beta 58
Vesicle protein sorting 26A
Short name=mVPS26

Gene names

Name:	Vps26a
Synonyms:	Vps26

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Ref.4
Subunit structure	Component of the retromer complex composed of VPS26 (VPS26A or VPS26B), VPS29, VPS35, SNX1 and SNX2. Interacts directly with VPS35. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN By similarity. Interacts with ECM29 By similarity.
Subcellular location	Cytoplasm. Endosome membrane; Peripheral membrane protein Ref.4.
Sequence similarities	Belongs to the VPS26 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Endosome Membrane
Coding sequence diversity	Alternative splicing
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW retrograde transport, endosome to Golgi Inferred from direct assay Ref.4. Source: MGI vacuolar transport Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from sequence or structural similarity. Source: UniProtKB endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell retromer complex Inferred from electronic annotation. Source: InterPro vesicle Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P40336-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P40336-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MSEPLPAFLDRLWGPWLGTRSPPSRSSAASPSK

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Vacuolar protein sorting-associated protein 26A		PRO_0000073008
Natural variations
	Alternative sequence	1	1	M → MSEPLPAFLDRLWGPWLGTR SPPSRSSAASPSK in isoform 2.		VSP_019926
Experimental info
	Sequence conflict	37	1	E → Q in BAC25745. Ref.2
	Sequence conflict	71	1	E → G in BAE35367. Ref.2
	Sequence conflict	79	1	F → L in BAE40171. Ref.2
	Sequence conflict	188	1	K → R in BAE40415. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 419DAED54264AC13	FASTA	327	38,114
	10 20 30 40 50 60 MSFLGGFFGP ICEIDVALND GETRKMAEMK TEDGKVEKHY LFYDGESVSG KVNLAFKQPG 70 80 90 100 110 120 KRLEHQGIRI EFVGQIELFN DKSNTHEFVN LVKELALPGE LTQSRSYDFE FMQVEKPYES 130 140 150 160 170 180 YIGANVRLRY FLKVTIVRRL TDLVKEYDLI VHQLATYPDV NNSIKMEVGI EDCLHIEFEY 190 200 210 220 230 240 NKSKYHLKDV IVGKIYFLLV RIKIQHMELQ LIKKEITGIG PSTTTETETI AKYEIMDGAP 250 260 270 280 290 300 VKGESIPIRL FLAGYDPTPT MRDVNKKFSV RYFLNLVLVD EEDRRYFKQQ EIILWRKAPE 310 320 KLRKQRTNFH QRFESPDSQA SAEQPEM
	Isoform 2 [UniParc]. Checksum: E09FDBE565B7E20B	FASTA	359	41,548
	10 20 30 40 50 60 MSEPLPAFLD RLWGPWLGTR SPPSRSSAAS PSKSFLGGFF GPICEIDVAL NDGETRKMAE 70 80 90 100 110 120 MKTEDGKVEK HYLFYDGESV SGKVNLAFKQ PGKRLEHQGI RIEFVGQIEL FNDKSNTHEF 130 140 150 160 170 180 VNLVKELALP GELTQSRSYD FEFMQVEKPY ESYIGANVRL RYFLKVTIVR RLTDLVKEYD 190 200 210 220 230 240 LIVHQLATYP DVNNSIKMEV GIEDCLHIEF EYNKSKYHLK DVIVGKIYFL LVRIKIQHME 250 260 270 280 290 300 LQLIKKEITG IGPSTTTETE TIAKYEIMDG APVKGESIPI RLFLAGYDPT PTMRDVNKKF 310 320 330 340 350 SVRYFLNLVL VDEEDRRYFK QQEIILWRKA PEKLRKQRTN FHQRFESPDS QASAEQPEM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a novel, evolutionarily conserved gene disrupted by the murine H beta 58 embryonic lethal transgene insertion." Lee J.J., Radice G., Perkins C.P., Costantini F. Development 115:277-288(1992) [PubMed: 1638986] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: C57BL/6J and DBA/2. Tissue: Amnion, Bone marrow, Lung, Placenta and Testis.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Mammary tumor.
[4]	"Cargo-selective endosomal sorting for retrieval to the Golgi requires retromer." Seaman M.N.J. J. Cell Biol. 165:111-122(2004) [PubMed: 15078902] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S41204 mRNA. Translation: AAB22718.1. AK028096 mRNA. Translation: BAC25745.1. AK150244 mRNA. Translation: BAE29408.1. AK147989 mRNA. Translation: BAE28271.1. AK151360 mRNA. Translation: BAE30335.1. AK159783 mRNA. Translation: BAE35367.1. AK167592 mRNA. Translation: BAE39650.1. AK165853 mRNA. Translation: BAE38415.1. AK168213 mRNA. Translation: BAE40171.1. AK168537 mRNA. Translation: BAE40415.1. BC007148 mRNA. Translation: AAH07148.1.
IPI	IPI00329942. IPI00776216.
PIR	A44882. B44882.
RefSeq	NP_001106826.1. NM_001113355.1. NP_598433.1. NM_133672.3.
UniGene	Mm.260703. Mm.473468.
3D structure databases
ProteinModelPortal	P40336.
SMR	P40336. Positions 6-299.
ModBase	Search...
Protein-protein interaction databases
IntAct	P40336. 1 interaction.
STRING	P40336.
PTM databases
PhosphoSite	P40336.
Proteomic databases
PRIDE	P40336.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000092473; ENSMUSP00000090130; ENSMUSG00000020078. ENSMUST00000105447; ENSMUSP00000101087; ENSMUSG00000020078.
GeneID	30930.
KEGG	mmu:30930.
UCSC	uc007fhf.2. mouse. uc007fhg.2. mouse.
Organism-specific databases
CTD	9559.
MGI	MGI:1353654. Vps26a.
Phylogenomic databases
eggNOG	roNOG07380.
GeneTree	ENSGT00390000002588.
HOVERGEN	HBG082914.
OMA	PVCEIDV.
OrthoDB	EOG470THM.
PhylomeDB	P40336.
Gene expression databases
ArrayExpress	P40336.
Bgee	P40336.
CleanEx	MM_VPS26A.
Genevestigator	P40336.
GermOnline	ENSMUSG00000020078. Mus musculus.
Family and domain databases
InterPro	IPR005377. VPS26. [Graphical view]
Pfam	PF03643. Vps26. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	307338.
SOURCE	Search...

Entry information

Entry name

VP26A_MOUSE

Accession

Primary (citable) accession number: P40336
Secondary accession number(s): Q3TGY3 , Q3THM5, Q3TW99, Q3UD54, Q8C1E9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	September 21, 2011
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program