P40335 (PEP8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carboxypeptidase Y-deficient protein 8

Alternative name(s):
Vacuolar protein sorting-associated protein 26
Vacuolar protein-targeting protein 4

Gene names

Name:	PEP8
Synonyms:	GRD6, VPS26, VPT4
Ordered Locus Names:	YJL053W
ORF Names:	J1152

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in vesicular protein sorting. Required for the endosome-to-Golgi retrieval of the vacuolar protein sorting receptor VPS10. Component of the membrane-associated retromer complex which is essential in endosome-to-Golgi retrograde transport. The VPS29-VPS26-VPS35 subcomplex may be involved in cargo selection.
Subunit structure	Component of the retromer complex which consists of VPS29, VPS26, VPS35, VPS5 and VPS17. Component of a retromer subcomplex consisting of VPS29, VPS26 and VPS35.
Sequence similarities	Belongs to the VPS26 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein retention in Golgi apparatus Inferred from mutant phenotype. Source: SGD protein transport Inferred from mutant phenotype Ref.5. Source: GOC retrograde transport, endosome to Golgi Inferred from physical interaction Ref.5. Source: SGD vacuolar transport Inferred from electronic annotation. Source: InterPro
Cellular component	endosome Inferred from physical interaction Ref.5. Source: SGD retromer complex, inner shell Inferred from mutant phenotype Ref.5. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
VPS5	Q92331	3	EBI-20373,EBI-20483

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	Carboxypeptidase Y-deficient protein 8		PRO_0000073015
Experimental info
	Sequence conflict	189	1	A → E in AAA34853. Ref.1
	Sequence conflict	286	1	R → K in AAT93026. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P40335 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 40D15594E70A9B58	FASTA	379	42,547
	10 20 30 40 50 60 MSIFFKPPID IEILFDNEES RKHVDIATRS SNSSYKSMKE SLPVYEDGES LGGIVTLRVR 70 80 90 100 110 120 DSKKVDHLGI KVSVIGSIDM LKSHGSGNSS SKKVTSSTSS SSSNGSVDVR KNSVDQFLCQ 130 140 150 160 170 180 SYDLCPAGEL QHSQSFPFLF RDLSKRYESY KGKNVDVAYY VKVTVMRKST DISKIKRFWV 190 200 210 220 230 240 YLYNSVTTAP NTLSANETKA TTNDIAGGNY AADNASDNTQ TKSTQGEAAD VNQVLPISHS 250 260 270 280 290 300 NNEPKPVRLD IGIENCLHIE FEYAKSQYSL KEVIVGRIYF LLTRLRIKHM ELSLITRESS 310 320 330 340 350 360 GLQTSNVMTD STAIRYEIMD GSSVKGETIP IRLFLSGYDL TPNMSCNYFN VKNYLSLVII 370 DEDGRRYFKQ SEITLYRTR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The yeast homolog of H<beta>58, a mouse gene essential for embryogenesis, performs a role in the delivery of proteins to the vacuole." Bachhawat A.K., Suhan J., Jones E.W. Genes Dev. 8:1379-1387(1994) [PubMed: 7926738] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 26109 / X2180.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"A membrane coat complex essential for endosome-to-Golgi retrograde transport in yeast." Seaman M.N., McCaffery J.M., Emr S.D. J. Cell Biol. 142:665-681(1998) [PubMed: 9700157] [Abstract] Cited for: IDENTIFICATION IN THE RETROMER COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L11623 Genomic DNA. Translation: AAA34853.1. Z49328 Genomic DNA. Translation: CAA89344.1. AY693007 Genomic DNA. Translation: AAT93026.1. BK006943 Genomic DNA. Translation: DAA08745.1.
PIR	S56825.
RefSeq	NP_012482.1. NM_001181486.1.
3D structure databases
ProteinModelPortal	P40335.
SMR	P40335. Positions 8-378.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4572N.
IntAct	P40335. 9 interactions.
MINT	MINT-491288.
STRING	P40335.
Proteomic databases
PeptideAtlas	P40335.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL053W; YJL053W; YJL053W.
GeneID	853393.
KEGG	sce:YJL053W.
NMPDR	fig|4932.3.peg.3454.
Organism-specific databases
CYGD	YJL053w.
SGD	S000003589. PEP8.
Phylogenomic databases
eggNOG	fuNOG04777.
HOGENOM	HBG525567.
OMA	SLIRRET.
OrthoDB	EOG40CMRZ.
Gene expression databases
ArrayExpress	P40335.
Genevestigator	P40335.
GermOnline	YJL053W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005377. VPS26. [Graphical view]
Pfam	PF03643. Vps26. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973870.

Entry information

Entry name

PEP8_YEAST

Accession

Primary (citable) accession number: P40335
Secondary accession number(s): D6VWC9, E9P910

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 14, 2011
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program