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P40329

- SYRC_RAT

UniProt

P40329 - SYRC_RAT

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Protein
Arginine--tRNA ligase, cytoplasmic
Gene
Rars
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1 By similarity.UniRule annotation

Catalytic activityi

ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).UniRule annotation

GO - Molecular functioni

  1. ATP binding Source: RGD
  2. arginine binding Source: RGD
  3. arginine-tRNA ligase activity Source: RGD
  4. tRNA binding Source: RGD
Complete GO annotation...

GO - Biological processi

  1. arginyl-tRNA aminoacylation Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_212517. Cytosolic tRNA aminoacylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Arginine--tRNA ligase, cytoplasmic (EC:6.1.1.19)
Alternative name(s):
Arginyl-tRNA synthetase
Short name:
ArgRS
Gene namesi
Name:Rars
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 10

Organism-specific databases

RGDi1309215. Rars.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. aminoacyl-tRNA synthetase multienzyme complex Source: RGD
  2. mitochondrion Source: Ensembl
  3. nucleus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 660660Arginine--tRNA ligase, cytoplasmicUniRule annotation
PRO_0000151660Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiP40329.

Expressioni

Gene expression databases

GenevestigatoriP40329.

Interactioni

Subunit structurei

Interacts (via N-terminus) with AIMP1 (via N-terminus); this stimulates its catalytic activity. Interacts with EPRS (via N-terminus). Interacts with LARS2. Monomer; also part of a multisubunit complex that groups tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro By similarity.

Protein-protein interaction databases

BioGridi252058. 1 interaction.
STRINGi10116.ENSRNOP00000010252.

Structurei

3D structure databases

ProteinModelPortaliP40329.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 7272Could be involved in the assembly of the multisynthetase complex By similarity
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi201 – 21212"HIGH" region By similarity
Add
BLAST

Domaini

The N-terminus (AA 1-72) has two regions predicted to be alpha-helical that might be involved in the multisynthetase complex assembly By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00530000063407.
HOGENOMiHOG000247212.
HOVERGENiHBG029238.
KOiK01887.
OMAiKCFDILG.
OrthoDBiEOG764725.
PhylomeDBiP40329.
TreeFamiTF106111.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.30.1360.70. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00123. Arg_tRNA_synth.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR001278. Arg-tRNA-ligase.
IPR005148. Arg-tRNA-synth_N.
IPR008909. DALR_anticod-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PANTHERiPTHR11956. PTHR11956. 1 hit.
PfamiPF03485. Arg_tRNA_synt_N. 1 hit.
PF05746. DALR_1. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
[Graphical view]
PRINTSiPR01038. TRNASYNTHARG.
SMARTiSM01016. Arg_tRNA_synt_N. 1 hit.
SM00836. DALR_1. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF55190. SSF55190. 1 hit.
TIGRFAMsiTIGR00456. argS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40329-1 [UniParc]FASTAAdd to Basket

« Hide

MDGLVAQCSA RLLQQEKEIK SLTAEIDRLK NCSYLEASPS LEQLREENLK    50
LKYRLNILRR SLQAERKRPT KNMININSRL QELFGCAIKA AYPDLENPPL 100
VVTPSQQPKF GDYQCNSAMG ISQMLKAKEQ KVSPREIAEN ITKHLPNNEY 150
IDRVEIAGPG FINVHLRKDF VSEQLTNLLV NGVQLPVLGE NEKVIVDFSS 200
PNIAKEMHVG HLRSTIIGES MSRLFEFAGY NVLRLNHVGD WGTQFGMLIA 250
HLQDKFPDYL TVSPPIGDLQ AFYKESKKRF DTEEEFKKRA YECVVLLQSK 300
NPDIMKAWNL ICDVSREEFN KIYDALDITL IERGESFYQD RMKDIVKEFE 350
DKGFVQVDDG RKIVFVPGCS VPLTIVKSDG GYTYDTSDLA AIKQRLFEEK 400
ANKIIYVVDN GQATHFQTVF AAAQMIGWYD PKVTRVAHVG FGVVLGEDKK 450
KFKTRSGETV RLMDLLEEGL RRSMDKLKEK ERDKVLTEEE LKAAQTSVAY 500
GCIKYADLSH NRLNDYVFSF DKMLDDRGNT AAYLLYAFTR IRSIARLANI 550
DEEMLQRAAR ETKIILDHEK EWKLGRCILR FPEILQKILD DLFLHTLCDY 600
IYELATTFTE FYDSCYCVEK DRQTGKVLKV NMWRMLLCEA VAAVMAKGFD 650
ILGIKPVQRM 660
Length:660
Mass (Da):75,810
Last modified:July 13, 2010 - v2
Checksum:i3C2D718AECDC4655
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC161929 mRNA. Translation: AAI61929.1.
PIRiA35857.
RefSeqiNP_001099247.2. NM_001105777.2.
UniGeneiRn.18711.

Genome annotation databases

EnsembliENSRNOT00000010252; ENSRNOP00000010252; ENSRNOG00000007739.
GeneIDi287191.
KEGGirno:287191.
UCSCiRGD:1309215. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC161929 mRNA. Translation: AAI61929.1 .
PIRi A35857.
RefSeqi NP_001099247.2. NM_001105777.2.
UniGenei Rn.18711.

3D structure databases

ProteinModelPortali P40329.
ModBasei Search...

Protein-protein interaction databases

BioGridi 252058. 1 interaction.
STRINGi 10116.ENSRNOP00000010252.

Proteomic databases

PRIDEi P40329.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000010252 ; ENSRNOP00000010252 ; ENSRNOG00000007739 .
GeneIDi 287191.
KEGGi rno:287191.
UCSCi RGD:1309215. rat.

Organism-specific databases

CTDi 5917.
RGDi 1309215. Rars.

Phylogenomic databases

GeneTreei ENSGT00530000063407.
HOGENOMi HOG000247212.
HOVERGENi HBG029238.
KOi K01887.
OMAi KCFDILG.
OrthoDBi EOG764725.
PhylomeDBi P40329.
TreeFami TF106111.

Enzyme and pathway databases

Reactomei REACT_212517. Cytosolic tRNA aminoacylation.

Miscellaneous databases

NextBioi 625686.

Gene expression databases

Genevestigatori P40329.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.30.1360.70. 1 hit.
3.40.50.620. 1 hit.
HAMAPi MF_00123. Arg_tRNA_synth.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR001278. Arg-tRNA-ligase.
IPR005148. Arg-tRNA-synth_N.
IPR008909. DALR_anticod-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view ]
PANTHERi PTHR11956. PTHR11956. 1 hit.
Pfami PF03485. Arg_tRNA_synt_N. 1 hit.
PF05746. DALR_1. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
[Graphical view ]
PRINTSi PR01038. TRNASYNTHARG.
SMARTi SM01016. Arg_tRNA_synt_N. 1 hit.
SM00836. DALR_1. 1 hit.
[Graphical view ]
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF55190. SSF55190. 1 hit.
TIGRFAMsi TIGR00456. argS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Brown Norway/NHsdMcwi.
    Tissue: Liver.
  2. "Existence of two forms of rat liver arginyl-tRNA synthetase suggests channeling of aminoacyl-tRNA for protein synthesis."
    Sivaram P., Deutscher M.P.
    Proc. Natl. Acad. Sci. U.S.A. 87:3665-3669(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 73-92.

Entry informationi

Entry nameiSYRC_RAT
AccessioniPrimary (citable) accession number: P40329
Secondary accession number(s): B1WBX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 13, 2010
Last modified: September 3, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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